NEK2 (NM_002497) Human Untagged Clone

CAT#: SC323443

NEK2 (untagged)-Kinase deficient mutant (K37M) of Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2)


  "NM_002497" in other vectors (7)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "NEK2"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NEK2
Synonyms HsPK21; NEK2A; NLK1; PPP1R111; RP67
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323443 sequence for NM_002497 edited (data generated by NextGen Sequencing)
ATGCCTTCCCGGGCGGAGGACTATGAAGTGTTGTACACCATTGGCACAGGCTCCTACGGC
CGCTGCCAGAAGATCCGGAGGAAGAGTGATGGCAAGATATTAGTTTGGATGGAACTTGAC
TATGGCTCCATGACAGAAGCTGAGAAACAGATGCTTGTTTCTGAAGTGAATTTGCTTCGT
GAACTGAAACATCCAAACATCGTTCGTTACTATGATCGGATTATTGACCGGACCAATACA
ACACTGTACATTGTAATGGAATATTGTGAAGGAGGGGATCTGGCTAGTGTAATTACAAAG
GGAACCAAGGAAAGGCAATACTTAGATGAAGAGTTTGTTCTTCGAGTGATGACTCAGTTG
ACTCTGGCCCTGAAGGAATGCCACAGACGAAGTGATGGTGGTCATACCGTATTGCATCGG
GATCTGAAACCAGCCAATGTTTTCCTGGATGGCAAGCAAAACGTCAAGCTTGGAGACTTT
GGGCTAGCTAGAATATTAAACCACGACACGAGTTTTGCAAAAACATTTGTTGGCACACCT
TATTACATGTCTCCTGAACAAATGAATCGCATGTCCTACAATGAGAAATCAGATATCTGG
TCATTGGGCTGCTTGCTGTATGAGTTATGTGCATTAATGCCTCCATTTACAGCTTTTAGC
CAGAAAGAACTCGCTGGGAAAATCAGAGAAGGCAAATTCAGGCGAATTCCATACCGTTAC
TCTGATGAATTGAATGAAATTATTACGAGGATGTTAAACTTAAAGGATTACCATCGACCT
TCTGTTGAAGAAATTCTTGAGAACCCTTTAATAGCAGATTTGGTTGCAGACGAGCAAAGA
AGAAATCTTGAGAGAAGAGGGCGACAATTAGGAGAGCCAGAAAAATCGCAGGATTCCAGC
CCTGTATTGAGTGAGCTGAAACTGAAGGAAATTCAGTTACAGGAGCGAGAGCGAGCTCTC
AAAGCAAGAGAAGAAAGATTGGAGCAGAAAGAACAGGAGCTTTGTGTTCGTGAGAGACTA
GCAGAGGACAAACTGGCTAGAGCAGAAAATCTGTTGAAGAACTACAGCTTGCTAAAGGAA
CGGAAGTTCCTGTCTCTGGCAAGTAATCCAGAACTTCTTAATCTTCCATCCTCAGTAATT
AAGAAGAAAGTTCATTTCAGTGGGGAAAGTAAAGAGAACATCATGAGGAGTGAGAATTCT
GAGAGTCAGCTCACATCTAAGTCCAAGTGCAAGGACCTGAAGAAAAGGCTTCACGCTGCC
CAGCTGCGTGCTCAAGCCCTGTCAGATATTGAGAAAAATTACCAACTGAAAAGCAGGCAG
ATCCTGGGCATGCGCTAG

Clone variation with respect to NM_002497.3
15 t=>g;110 a=>t;111 a=>g;504 t=>c;1269 g=>t;1317 a=>g
>OriGene 5' read for mutant NM_002497 unedited
ACCGCCGTTCAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGCGGGTCAGTGCTG
CTCGGGGGCTTCTCCATCCAGGTCCCTGGAGTTCCTGGTCCCTGGAGCTCCGCACTTGGCGGCGCAACCT
GCGTGAGGCAGCGCGACTCTGGCGACTGGCCGGCCATGCCTTCCCGGGCGGAGGACTATGAAGTGTTGTA
CACCATTTGGCACAGGCTCCTACGGCCGCTGCCAGAAAGATCCCGGAGGAAAGAGTGATGGGCAAGATAT
TAGTTTGGATGGGAACTTTGACTATGGGCTCCCATGACAGAAAGCTGAAGAAAACAGAATGCCTTTGTTT
CCTGAAAGTGATTTTGCCTCCTTGACCTGAACCATCCAAACCATCCGTTCCGTTACTATGATCCGGATTA
TGGACCGGACAATATAAACACATGGTCCATTGAAAGGAAAAATTGAAAGGAGGGGATCTGGCTATGGAAT
TTACAGGGGAACCAAGGAAAGGATTATTTATAAAAAGATTTTGTTTTTCGAGTATAACTCTTGACTCTGG
GCCCGAGAGAGAGCCCCAGCGAAAATGTTAGTGGGCCACCCCTATTGCGCTGGTCTCTGACCCGCGACTG
TGTTCTCTAGATGAGCACAACGTCTAGCGCGTGGACACTTGGCGCCTACTATATAACCCGAGCGAGTTTG
TCAAACACTTGTGTCCACACATAATACTCTGTCAATGATACATATGCTCACTGAATCATATCTGAATGGC
GCTCGGTGATATGGCTAAGCCATAGCTTGACAGAACTCTGGATAAGAGACGCAATTACGTTCTGAGTATG
GATTCAG
>SC323443 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CCCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGCGGGTCAGTGCTGCTCGG
GGGCTTCTCCATCCAGGTCCCTGGAGTTCCTGGTCCCTGGAGCTCCGCACTTGGCGGCGCAACCTGCGTG
AGGCAGCGCGACTCTGGCGACTGGCCGGCCATGCCTTCCCGGGCG
Restriction Sites Please inquire     
ACCN NM_002497
Insert Size 2210 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002497.2, NP_002488.1
RefSeq Size 2130 bp
RefSeq ORF 1338 bp
Locus ID 4751
Cytogenetics 1q32.3
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Gene Summary 'This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]'
Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1, also known as NEK2A). The C-terminus (aa 399-445) of this isoform was shown to be responsible for its nucleolar localization (PMID:15161910). Isoforms 1 and 2 (which differ at the C-terminus) have also been reported to exhibit distinct pattern of expression during mitosis (PMID:11742531).

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.