NEK2 (NM_002497) Human Untagged Clone

CAT#: SC323443

NEK2 (untagged)-Kinase deficient mutant (K37M) of Human NIMA (never in mitosis gene a)-related kinase 2 (NEK2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEK2
• Synonyms: HsPK21; NEK2A; NLK1; PPP1R111; RP67
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323443 sequence for NM_002497 edited (data generated by NextGen Sequencing)
  ATGCCTTCCCGGGCGGAGGACTATGAAGTGTTGTACACCATTGGCACAGGCTCCTACGGC
  CGCTGCCAGAAGATCCGGAGGAAGAGTGATGGCAAGATATTAGTTTGGATGGAACTTGAC
  TATGGCTCCATGACAGAAGCTGAGAAACAGATGCTTGTTTCTGAAGTGAATTTGCTTCGT
  GAACTGAAACATCCAAACATCGTTCGTTACTATGATCGGATTATTGACCGGACCAATACA
  ACACTGTACATTGTAATGGAATATTGTGAAGGAGGGGATCTGGCTAGTGTAATTACAAAG
  GGAACCAAGGAAAGGCAATACTTAGATGAAGAGTTTGTTCTTCGAGTGATGACTCAGTTG
  ACTCTGGCCCTGAAGGAATGCCACAGACGAAGTGATGGTGGTCATACCGTATTGCATCGG
  GATCTGAAACCAGCCAATGTTTTCCTGGATGGCAAGCAAAACGTCAAGCTTGGAGACTTT
  GGGCTAGCTAGAATATTAAACCACGACACGAGTTTTGCAAAAACATTTGTTGGCACACCT
  TATTACATGTCTCCTGAACAAATGAATCGCATGTCCTACAATGAGAAATCAGATATCTGG
  TCATTGGGCTGCTTGCTGTATGAGTTATGTGCATTAATGCCTCCATTTACAGCTTTTAGC
  CAGAAAGAACTCGCTGGGAAAATCAGAGAAGGCAAATTCAGGCGAATTCCATACCGTTAC
  TCTGATGAATTGAATGAAATTATTACGAGGATGTTAAACTTAAAGGATTACCATCGACCT
  TCTGTTGAAGAAATTCTTGAGAACCCTTTAATAGCAGATTTGGTTGCAGACGAGCAAAGA
  AGAAATCTTGAGAGAAGAGGGCGACAATTAGGAGAGCCAGAAAAATCGCAGGATTCCAGC
  CCTGTATTGAGTGAGCTGAAACTGAAGGAAATTCAGTTACAGGAGCGAGAGCGAGCTCTC
  AAAGCAAGAGAAGAAAGATTGGAGCAGAAAGAACAGGAGCTTTGTGTTCGTGAGAGACTA
  GCAGAGGACAAACTGGCTAGAGCAGAAAATCTGTTGAAGAACTACAGCTTGCTAAAGGAA
  CGGAAGTTCCTGTCTCTGGCAAGTAATCCAGAACTTCTTAATCTTCCATCCTCAGTAATT
  AAGAAGAAAGTTCATTTCAGTGGGGAAAGTAAAGAGAACATCATGAGGAGTGAGAATTCT
  GAGAGTCAGCTCACATCTAAGTCCAAGTGCAAGGACCTGAAGAAAAGGCTTCACGCTGCC
  CAGCTGCGTGCTCAAGCCCTGTCAGATATTGAGAAAAATTACCAACTGAAAAGCAGGCAG
  ATCCTGGGCATGCGCTAG
  
  Clone variation with respect to NM_002497.3
  15 t=>g;110 a=>t;111 a=>g;504 t=>c;1269 g=>t;1317 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002497 unedited
  ACCGCCGTTCAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGCGGGTCAGTGCTG
  CTCGGGGGCTTCTCCATCCAGGTCCCTGGAGTTCCTGGTCCCTGGAGCTCCGCACTTGGCGGCGCAACCT
  GCGTGAGGCAGCGCGACTCTGGCGACTGGCCGGCCATGCCTTCCCGGGCGGAGGACTATGAAGTGTTGTA
  CACCATTTGGCACAGGCTCCTACGGCCGCTGCCAGAAAGATCCCGGAGGAAAGAGTGATGGGCAAGATAT
  TAGTTTGGATGGGAACTTTGACTATGGGCTCCCATGACAGAAAGCTGAAGAAAACAGAATGCCTTTGTTT
  CCTGAAAGTGATTTTGCCTCCTTGACCTGAACCATCCAAACCATCCGTTCCGTTACTATGATCCGGATTA
  TGGACCGGACAATATAAACACATGGTCCATTGAAAGGAAAAATTGAAAGGAGGGGATCTGGCTATGGAAT
  TTACAGGGGAACCAAGGAAAGGATTATTTATAAAAAGATTTTGTTTTTCGAGTATAACTCTTGACTCTGG
  GCCCGAGAGAGAGCCCCAGCGAAAATGTTAGTGGGCCACCCCTATTGCGCTGGTCTCTGACCCGCGACTG
  TGTTCTCTAGATGAGCACAACGTCTAGCGCGTGGACACTTGGCGCCTACTATATAACCCGAGCGAGTTTG
  TCAAACACTTGTGTCCACACATAATACTCTGTCAATGATACATATGCTCACTGAATCATATCTGAATGGC
  GCTCGGTGATATGGCTAAGCCATAGCTTGACAGAACTCTGGATAAGAGACGCAATTACGTTCTGAGTATG
  GATTCAG
  
  Kinase domain sequence

  >SC323443 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CCCTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGGCGGGTCAGTGCTGCTCGG
  GGGCTTCTCCATCCAGGTCCCTGGAGTTCCTGGTCCCTGGAGCTCCGCACTTGGCGGCGCAACCTGCGTG
  AGGCAGCGCGACTCTGGCGACTGGCCGGCCATGCCTTCCCGGGCG
  
• Restriction Sites: Please inquire
• ACCN: NM_002497
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002497.2, NP_002488.1
• RefSeq Size: 2130 bp
• RefSeq ORF: 1338 bp
• Locus ID: 4751
• Cytogenetics: 1q32.3
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: 'This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longest isoform (1, also known as NEK2A). The C-terminus (aa 399-445) of this isoform was shown to be responsible for its nucleolar localization (PMID:15161910). Isoforms 1 and 2 (which differ at the C-terminus) have also been reported to exhibit distinct pattern of expression during mitosis (PMID:11742531).