MGMT (NM_002412) Human Untagged Clone

CAT#: SC108494

MGMT (untagged)-Human O-6-methylguanine-DNA methyltransferase (MGMT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MGMT
• Synonyms: methylguanine-DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; OTTHUMP00000020741
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002412, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGGGACAGCCCGCGCCCCTAGAACGCTTTGCGTCCCGACGCCCGCAGGTCCTCGCGGTGCGCACCG
  TTTGCGACTTGGTACTTGGAAAAATGGACAAGGATTGTGAAATGAAACGCACCACACTGGACAGCCCTTT
  GGGGAAGCTGGAGCTGTCTGGTTGTGAGCAGGGTCTGCACGAAATAAAGCTCCTGGGCAAGGGGACGTCT
  GCAGCTGATGCCGTGGAGGTCCCAGCCCCCGCTGCGGTTCTCGGAGGTCCGGAGCCCCTGATGCAGTGCA
  CAGCCTGGCTGAATGCCTATTTCCACCAGCCCGAGGCTATCGAAGAGTTCCCCGTGCCGGCTCTTCACCA
  TCCCGTTTTCCAGCAAGAGTCGTTCACCAGACAGGTGTTATGGAAGCTGCTGAAGGTTGTGAAATTCGGA
  GAAGTGATTTCTTACCAGCAATTAGCAGCCCTGGCAGGCAACCCCAAAGCCGCGCGAGCAGTGGGAGGAG
  CAATGAGAGGCAATCCTGTCCCCATCCTCATCCCGTGCCACAGAGTGGTCTGCAGCAGCGGAGCCGTGGG
  CAACTACTCCGGAGGACTGGCCGTGAAGGAATGGCTTCTGGCCCATGAAGGCCACCGGTTGGGGAAGCCA
  GGCTTGGGAGGGAGCTCAGGTCTGGCAGGGGCCTGGCTCAAGGGAGCGGGAGCTACCTCGGGCTCCCCGC
  CTGCTGGCCGAAACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002412 unedited
  CGTCAACATTTGTATACGACTCATATAGGCGGCCGCGCCTCGTGCCGAATTCGGCACGAG
  GCTGGGACAGCCCGCGCCCCTAGAACGCTTTGCGTCCCGACGCCCGCAGGTCCTCGCGGT
  GCGCACCGTTTGCGACTTGGTACTTGGAAAAATGGACAAGGATTGTGAAATGAAACGCAC
  CACACTGGACAGCCCTTTGGGGAAGCTGGAGCTGTCTGGTTGTGAGCAGGGTCTGCACGA
  AATAAAGCTCCTGGGCAAGGGGACGTCTGCAGCTGATGCCGTGGAGGTCCCAGCCCCCGC
  TGCGGTTCTCGGAGGTCCGGAGCCCCTGATGCAGTGCACAGCCTGGCTGAATGCCTATTT
  CCACCAGCCCGAGGCTATCGAAGAGTTCCCCGTGCCGGCTCTTCACCATCCCGTTTTCCA
  GCAAGAGTCGTTCACCAGACAGGTGTTATGGAAGCTGCTGAAGGTTGTGAAATTCGGAGA
  AGTGATTTCTTACCAGCAATTAGCAGCCCTGGCAGGCAACCCCAAAGCCGCGCGAGCAGT
  GGGAGGAGCAATGAGAGGCAATCCTGTCCCCATCCTCATCCCGTGCCACAGAGTGGTCTG
  CAGCAGCGGAGCCGTGGGCAACTACTCCGGAGGACTGGCCGTGAAGGAATGGCTTCTGGC
  CCATGAAGGCCACCGGTTGCGGAAGCCAGGCTTGGGAGGGAGCTCANGTCTGGCAGGNGC
  CTGGCTCAAGGGAGCGGGAGCTACCTCGGGCTTCCCGCCTGCTGGCCGAACTGAGTATGT
  GCAGTCAGATGGATGTTTGAGCGACACACACGTGTTACACTGCATCGNATGCGGGGCGTG
  GNAGCCACCGCTGTATTAAAGGAAGGGGCCAGTGTCCTTGGGAAAAAAAAAAAAAAAAAA
  CTCGATTCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002412 unedited
  CGGCCGCAATCTANAATCGAGTTTTTTTTTTTTTTTTTTCCCAGGACACTGCCACTTCCT
  TTAATACAGCGGTGCCTCCACGCCCCGCATCCGATGCAGTGTTACACGTGTGTGTCGCTC
  AAACATCCATCCTACTGCACATACTCAGTTTCGGCCAGCAGGCGGGGAGCCCGAGGTAGC
  TCCCGCTCCCTTGAGCCAGGCCCCTGCCAGACCTGAGCTCCCTCCCAAGCCTGGCTTCCC
  CAACCGGTGGCCTTCATGGGCCAGAAGCCATTCCTTCACGGCCAGTCCTCCGGAGTAGTT
  GCCCACGGCTCCGCTGCTGCAGACCACTCTGTGGCACGGGATGAGGATGGGGACAGGATT
  GCCTCTCATTGCTCCTCCCACTGCTCGCGCGGCTTTGGGGTTGCCTGCCAGGGCTGCTAA
  TTGCTGGTAAGAAATCACTTCTCCGAATTTCACAACCTTCAGCAGCTTCCATAACACCTG
  TCTGGTGAACGACTCTTGCTGGAAAACGGGATGGTGAAGAGCCGGCACGGGGAACTCTTC
  GATAGCCTCGGGCTGGTGGAAATAGGCATTCAGCCAGGCTGTGCACTGCATCAGGGGCTC
  CGGACCTCCGAGAACCGCAGCGGGGGCTGGGACCTCCACGGCATCAGCTGCAGACGTCCC
  CTTGCCCAGGAGCTTTATTTCGTGCAGACCCTGCTCACAACCAGACAGCTCCAGCTTCCC
  CAAAGGGCTGTCCAGTGTGGTGCGTTTCATTTCACACTCCTTGTGCATTCTTCCAAGTAC
  CAAGTCGNAAACGGTGCGCACCGAGAGGACCTGCGGGCGTCGGGGCGCAAAGCGTTCTAG
  GGGCGCGGGCTGTCCCACCCTCGGGCCGATNCGGCACGAGCGCGGCCGGCCTTAAGGGAG
  TCGGATTAAAAATTCTGACGGTTCACTAACGAGCTCTGGCTATATAGACTCCCCCGGACA
  ACCTACCGCCATTGCGTAAAGGGCCGGGCTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002412
• Insert Size: 870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002412.2, NP_002403.1
• RefSeq Size: 1265 bp
• RefSeq ORF: 624 bp
• Locus ID: 4255
• Cytogenetics: 10q26.3
• Domains: Methyltransf_1
• Protein Families: Druggable Genome
• Gene Summary: 'Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]'