Glutathione Reductase (GSR) (NM_000637) Human Untagged Clone

CAT#: SC108586

GSR (untagged)-Human glutathione reductase (GSR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GSR
• Synonyms: GR; GSRD; HEL-75; HEL-S-122m
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000637, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCCTGCTGCCCCGAGCCCTGAGCGCCGGCGCGGGACCGAGCTGGCGGCGGGCGGCGCGCGCCTTCC
  GAGGCTTCCTGCTGCTTCTGCCCGAGCCCGCGGCCCTCACGCGCGCCCTCTCCCGTGCCATGGCCTGCAG
  GCAGGAGCCGCAGCCGCAGGGCCCGCCGCCCGCTGCTGGCGCCGTGGCCTCCTATGACTACCTGGTGATC
  GGGGGCGGCTCGGGCGGGCTGGCCAGCGCGCGCAGGGCGGCCGAGCTGGGTGCCAGGGCCGCCGTGGTGG
  AGAGCCACAAGCTGGGTGGCACTTGCGTGAATGTTGGATGTGTACCCAAAAAGGTAATGTGGAACACAGC
  TGTCCACTCTGAATTCATGCATGATCATGCTGATTATGGCTTTCCAAGTTGTGAGGGTAAATTCAATTGG
  CGTGTTATTAAGGAAAAGCGGGATGCCTATGTGAGCCGCCTGAATGCCATCTATCAAAACAATCTCACCA
  AGTCCCATATAGAAATCATCCGTGGCCATGCAGCCTTCACGAGTGATCCCAAGCCCACAATAGAGGTCAG
  TGGGAAAAAGTACACCGCCCCACACATCCTGATCGCCACAGGTGGTATGCCCTCCACCCCTCATGAGAGC
  CAGATCCCCGGTGCCAGCTTAGGAATAACCAGCGATGGATTTTTTCAGCTGGAAGAATTGCCCGGCCGCA
  GCGTCATTGTTGGTGCAGGTTACATTGCTGTGGAGATGGCAGGGATCCTGTCAGCCCTGGGTTCTAAGAC
  ATCACTGATGATACGGCATGATAAGGTACTTAGAAGTTTTGATTCAATGATCAGCACCAACTGCACGGAG
  GAGCTGGAGAACGCTGGCGTGGAGGTGCTGAAGTTCTCCCAGGTCAAGGAGGTTAAAAAGACTTTGTCGG
  GCTTGGAAGTCAGCATGGTTACTGCAGTTCCCGGTAGGCTACCAGTCATGACCATGATTCCAGATGTTGA
  CTGCCTGCTCTGGGCCATTGGGCGGGTCCCGAATACCAAGGACCTGAGTTTAAACAAACTGGGGATTCAA
  ACCGATGACAAGGGTCATATCATCGTAGACGAATTCCAGAATACCAACGTCAAAGGCATCTATGCAGTTG
  GGGATGTATGTGGAAAAGCTCTTCTTACTCCAGTTGCAATAGCTGCTGGCCGAAAACTTGCCCATCGACT
  TTTTGAATATAAGGAAGATTCCAAATTAGATTATAACAACATCCCAACTGTGGTCTTCAGCCACCCCCCT
  ATTGGGACAGTGGGACTCACGGAAGATGAAGCCATTCATAAATATGGAATAGAAAATGTGAAGACCTATT
  CAACGAGCTTTACCCCGATGTATCACGCAGTTACCAAAAGGAAAACAAAATGTGTGATGAAAATGGTCTG
  TGCTAACAAGGAAGAAAAGGTGGTTGGGATCCATATGCAGGGACTTGGGTGTGATGAAATGCTGCAGGGT
  TTTGCTGTTGCAGTGAAGATGGGAGCAACGAAGGCAGACTTTGACAACACAGTCGCCATTCACCCTACCT
  CTTCAGAAGAGCTGGTCACACTTCGTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000637 unedited
  GATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACCAGGCGGCGCGCGCC
  TTCCGAGGCTTCCTGCTGCTTCTGCCCGAGCCCGCGGCCCTCACGCGCGCCCTCTCCCGT
  GCCATGGCCTGCAGGCAGGAGCCGCAGCCGCAGGGCCCGCCGCCCGCTGCTGGCGCCGTG
  GCCTCCTATGACTACCTGGTGATCGGGGGCGGCTCGGGCGGGCTGGCCAGCGCGCGCAGG
  GCGGCCGAGCTGGGTGCCAGGGCCGCCGTGGTGGAGAGCCACAAGCTGGGTGGCACTTGC
  GTGAATGTTGGATGTGTACCCAAAAAGGTAATGTGGAACACAGCTGTCCACTCTGAATTC
  ATGCATGATCATGCTGATTATGGCTTTCCAAGTTGTGAGGGTAAATTCAATTGGCGTGTT
  ATTAAGGAAAAGCGGGATGCCTATGTGAGCCGCCTGAATGCCATCTATCAAAACAATCTC
  ACCAAGTCCCATATAGAAATCATCCGTGGCCATGCAGCCTTCACGAGTGATCCCAAGCCC
  ACAATAGAGGTCAGTGGGAAAAAGTACACCGCCCCACACATCCTGATCGCCACAGGTGGT
  ATGCCCTCCACCCCTCATGAGAGCCAGATCCCCGGTGCCAGCTTAGGAATAACCAGCGAT
  GGATTTTTTCAGCTGGAAGAATTGCCCGGCCGCAGCGTCATTGTTGGTGCANGTTACATT
  GCTGTGGAGATGGCAGGNATCCTGTCAGCCCTGNGTTCTAAGACATCACTGATGATACCG
  CATGATAAGGTACTTTAGAGTTTTGATTCANTGATCAGCACCAACTGCACGNGAGAGCTG
  GAGAACGCNNTGCGTGNAGTGCTGAAGTTCTNCCAGGTCAAGGAGGTAAAAAAGACTTGT
  CGGNCTTNGAAGTCANCATGGNTACTGCAGTTTCCGGGTAGCTACCAGTCATGACCCATG
  ATCCCAGTGTTGACTGCCTGCTCTGGGCCATTGGCGGNTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000637 unedited
  GCCCATTTAGNACGCGCCGCATTTANGATCGGTTTTTTTTTTTTTTTTTTGTAAGTCAAT
  GTGATTATTTGTTTCATTTCAGAAGATCTATGGGTCCCACTGCCCGCCACACGTGTCTCC
  TGGTTCTCAACGAAGTGTGACCAGCTCTTCTGAAGAGGTAGGGTGAATGGCGACTGTGTT
  GTCAAAGTCTGCCTTCGTTGCTCCCATCTTCACTGCAACAGCAAAACCCTGCAGCATTTC
  ATCACACCCAAGTCCCTGCATATGGATCCCAACCACCTTTTCTTCCTTGTTAGCACAGAC
  CATTTTCATCACACATTTTGTTTTCCTTTTGGTAACTGCGTGATACATCGGGGTAAAGCT
  CGTTGAATAGGTCTTCACATTTTCTATTCCATATTTATGAATGGCTTCATCTTCCGTGAG
  TCCCACTGTCCCAATAGGGGGGTGGCTGAAGACCACAGTTGGGATGTTGTTATAATCTAA
  TTTGGAATCTTCCTTATATTCAAAAAGTCGATGGGCAAGTTTTCGGCCAGCAGCTATTGC
  AACTGGAGTAAGAAGAGCTTTTCCACATACATCCCCAACTGCATAGATGCCTTTGACGTT
  GGTATTCTGGAATTCGTCTACGATGATATGACCCTTGTCATCGGTTTGAATCCCCAGTTT
  GTTTAAACTCANGTCCTTGGTATTCGGGACCCGCCCAATGGCCCAGAGCANGCAGTCAAC
  ATCTGGAATCATGGTCATGACTGGTAGCCTACCGGGAACTGCAGTAACCATGCTGACTTC
  CNAGCCCGACAAAAGTCTTTTAAACTCCTTGACCTGGGAGAACTTCAGCACCTCCACGCC
  AGCGTTCTCCAGCTCCTNCGTGCAGTTGGTGCTGATCATTGAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000637
• Insert Size: 1900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000637.1, NP_000628.1
• RefSeq Size: 3174 bp
• RefSeq ORF: 3174 bp
• Locus ID: 2936
• Cytogenetics: 8p12
• Domains: pyr_redox, pyr_redox_dim
• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism
• Gene Summary: 'This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. This enzyme is a homodimeric flavoprotein. It is a central enzyme of cellular antioxidant defense, and reduces oxidized glutathione disulfide (GSSG) to the sulfhydryl form GSH, which is an important cellular antioxidant. Rare mutations in this gene result in hereditary glutathione reductase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2010]'
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.