VPS54 (NM_016516) Human Untagged Clone

CAT#: SC108738

VPS54 (untagged)-Human vacuolar protein sorting 54 homolog (S. cerevisiae) (VPS54), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VPS54
• Synonyms: HCC8; hVps54L; PPP1R164; SLP-8p; VPS54L; WR
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108738 sequence for NM_016516 edited (data generated by NextGen Sequencing)
  ATGGCTTCAAGCCACAGTTCTTCACCAGTGCCTCAAGGAAGCAGCAGTGATGTTTTCTTT
  AAAATAGAGGTAGATCCGTCAAAACACATTCGACCTGTGCCATCACTGCCAGATGTGTGT
  CCCAAGGAACCCACAGGTGATTCACATAGTTTATATGTTGCCCCATCTCTAGTTACAGAT
  CAACATAGATGGACTGTATATCATTCCAAAGTAAATCTCCCAGCAGCATTAAACGATCCT
  AGATTAGCAAAAAGAGAATCTGACTTCTTCACAAAAACATGGGGATTGGACTTTGTGGAC
  ACTGAAGTCATACCTTCATTCTACCTCCCACAGATCAGCAAGGAACATTTTACAGTATAT
  CAACAGGAAATCTCTCAGAGAGAGAAGATTCATGAGAGATGCAAGAATATTTGTCCTCCT
  AAAGATACCTTCGAAAGGACTCTTTTACATACTCATGATAAATCCAGGACAGATCTGGAG
  CAAGTACCTAAGATTTTTATGAAACCAGATTTTGCCTTGGATGATTCCTTAACTTTTAAT
  TCAGTTTTACCATGGTCTCATTTTAATACTGCTGGTGGAAAAGGAAATCGTGATGCAGCT
  TCCTCAAAGTTGCTTCAAGAAAAGCTGAGCCATTATCTGGATATTGTGGAAGTAAACATT
  GCTCACCAGATCTCTCTACGTTCAGAAGCATTTTTTCATGCAATGACCTCTCAACACGAG
  TTGCAGGACTACCTCAGGAAAACTTCCCAGGCTGTAAAAATGCTTCGAGATAAAATTGCA
  CAGATTGATAAAGTAATGTGTGAAGGATCACTCCACATTTTAAGACTGGCACTTACCAGA
  AATAATTGTGTTAAAGTATACAATAAGCTGAAGTTAATGGCCACTGTACACCAGACTCAG
  CCTACAGTACAGGTGTTATTATCTACTTCTGAATTTGTTGGAGCATTGGACTTAATAGCA
  ACAACACAAGAGGTTCTACAGCAGGAACTTCAGGGCATTCACAGTTTCCGGCATTTGGGA
  TCACAGCTTTGTGAATTAGAAAAACTGATAGATAAAATGATGATTGCAGAATTTTCTACT
  TATTCTCACAGTGACTTAAATAGACCACTGGAAGATGACTGTCAAGTTTTAGAAGAGGAA
  AGACTAATATCTCTTGTATTTGGACTTTTAAAACAAAGAAAGCTTAATTTTTTAGAAATC
  TATGGTGAAAAAATGGTTATTACAGCAAAGAATATCATTAAACAGTGTGTGATTAATAAA
  GTTTCACAAACAGAAGAAATAGACACAGATGTTGTTGTGAAGCTTGCAGATCAGATGAGA
  ATGTTGAATTTTCCCCAGTGGTTTGATCTGCTCAAGGATATTTTCTCTAAGTTTACAATT
  TTCCTACAGAGAGTGAAGGCAACATTAAATATCATTCACAGTGTTGTTCTCTCAGTTCTT
  GACAAAAACCAAAGGACTAGAGAATTGGAAGAGATTTCACAACAGAAGAATGCTGCAAAA
  GATAATTCACTGGACACAGAGGTGGCTTATTTAATCCATGAAGGCATGTTTATAAGTGAT
  GCATTCGGTGAGGGTGAGCTAACACCTATAGCAGTTGACACTACCTCTCAAAGAAATGCA
  TCTCCAAATAGTGAGCCCTGCAGCAGTGATTCTGTATCCGAGCCAGAATGTACTACTGAT
  TCTTCATCCAGCAAAGAGCACACATCATCATCTGCTATTCCAGGAGGTGTGGATATTATG
  GTCAGTGAAGATATGAAATTAACTGACTCAGAGCTAGGAAAGCTGGCAAATAATATCCAG
  GAATTATTATATAGTGCCTCAGATATATGCCATGATCGAGCTGTCAAATTTCTCATGTCA
  AGAGCAAAGGATGGTTTTCTTGAGAAGCTAAATTCCATGGAATTCATAACACTTTCTAGA
  TTAATGGAAACATTCATTTTAGACACCGAACAGATCTGTGGAAGAAAAAGCACGTCATTA
  CTTGGAGCACTTCAGAGCCAAGCTATTAAGTTTGTAAATAGGTTTCATGAAGAGAGAAAA
  ACCAAGCTCAGCCTCCTCTTAGACAATGAGCGCTGGAAGCAAGCAGATGTTCCTGCAGAA
  TTTCAGGATCTTGTTGATTCTCTGTCAGATGGGAAGATTGCTTTACCTGAAAAAAAATCA
  GGAGCCACAGAAGAAAGGAAACCAGCTGAAGTTCTTATTGTCGAGGGACAACAGTATGCA
  GTTGTTGGAACCGTATTGCTGTTAATAAGAATTATCCTTGAATATTGCCAGTGTGTGGAT
  AACATCCCATCTGTTACTACTGACATGCTTACTCGTCTGTCAGATTTATTGAAGTACTTC
  AATTCAAGAAGTTGCCAGTTAGTTCTTGGAGCTGGTGCACTGCAAGTTGTTGGACTAAAA
  ACGATAACTACAAAAAATTTGGCTCTTTCTTCACGATGTTTGCAGTTAATTGTGCACTAC
  ATTCCTGTGATCCGGGCTCATTTTGAAGCTCGACTACCACCTAAGCAATATAGCATGCTT
  AGGCATTTTGATCATATCACTAAGGACTACCATGATCACATAGCTGAAATATCAGCTAAG
  CTTGTAGCGATAATGGATAGCTTATTTGACAAGCTGTTATCTAAGTATGAAGTGAAGGCT
  CCTGTTCCTTCTGCCTGTTTCAGGAATATTTGTAAGCAAATGACAAAAATGCACGAAGCT
  ATATTTGATCTCCTTCCAGAAGAACAAACACAGATGTTATTTTTAAGAATTAATGCAAGT
  TATAAACTCCACTTGAAAAAGCAGTTATCTCACTTAAATGTGATAAATGATGGAGGACCT
  CAAAATGGGTTGGTCACAGCAGATGTAGCTTTTTACACTGGAAATCTTCAAGCCTTAAAA
  GGCCTTAAAGATTTGGACCTAAATATGGCCGAAATTTGGGAGCAGAAGAGGTGA
  
  Clone variation with respect to NM_016516.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016516 unedited
  NGGTTTTCAGGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGGCCACC
  TCTCACCCCTGAGTGAAGCTGGGCTCGAGAGGGCGGCCCTGTGCTCCCCGGGCCGACTGG
  CCAGCGGGCGCGGGGCGGGGGCGGGAACCCGGGCTCGGGCCCGGCCGGGCGCCGGGCGGC
  GGCGGCCGTGGAGCAGCAGCCTCGGTGCGACGTGGAGGGCTGGAGGCGGCGGCGATGCAC
  TAGGCCTCGCTCAGGGCGGCTGCCCCGGGACCCGCAGTTGAGTGGTGATTTTATGCAATG
  GCTTCAAGCCACAGTTCTTCACCAGTGCCTCAAGGAAGCAGCAGTGATGTTTTCTTTAAA
  ATAGAGGTAGATCCGTCAAAACACATTCGACCTGTGCCATCACTGCCAGATGTGTGTCCC
  AAGGAACCCACAGGTGATTCACATAGTTTATATGTTGCCCCATCTCTAGTTACAGATCAA
  CATAGATGGACTGTATATCATTCCAAAGTAAATCTCCCAGCAGCATTAAACGATCCTAGA
  TTAGCAAAAAGAGAATCTGACTTCTTCACAAAAACATGGGGATTGGACTTTGTGGACACT
  GAAGTCATACCTTCATTCTACCTCCCACAGATCAGCAAGGAACATTTTACAGTATATCAA
  CAGGAAATCTCTCAGAGAGAGAAGATTCATGAGAGATGCAAGAATATTTGTCCTCCTAAA
  GATACCTTCGAAGGACTCTTTTCATACTCATGATAAATCCAGGACAGATCTGGAGCCAGT
  ACCTAAGATTTTATGAAACCAGATTTGCCTCGGATGATCTCTTAACTTTAATNCAGTTTT
  CCATGGNCTCATTTTATACTGCTGGNGGNAAAGGAATTCGGAGCAGCTCCTTAAGTTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016516 unedited
  CTTTGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTAATTTT
  TTACAGCTTTATTTTAGACAGATAGTTTAAGAACCAAAGACATACCTCTGTAATGATAAA
  GGAAAGAAAACAAGCTTTCCTTTTAAGAAACCAAAGAGCACAAAATAAGACTGTTTCATT
  ATACATAATCACCACAGGATATTAGGCACTCTGACAGGGTTAGGCAAGATTCTTGGTGTG
  AGGTGAAGCACAGGCACTTTATTTGTACAGTGCTGCTGATTCTAATTTTGAAGGTAGGTA
  TTATAAAAGTCTTTACTTGTCACCTTATTTCTGGCCCCAACACAGCAGCCTATAGTTTTA
  AAAGTTCTGTTTCTCCCTGGTCTTTGTTCGTATACACATCGAAAGTAACTTAAAAACAAG
  GATCCAAGGGGGCCATACTTCATATGTTATCTAAATGTTAATATGAGAACTCAAAAGTAG
  GCAGATTATATGAATACATATTCTTACCTCTGCTACAAATAAAAACACCCCAAACCCTTC
  ATCATACTTTTATAAAAATACAGATATTAAACTGTAGCCTTTTATATACCATTTCTGAAA
  AGTATTTAGTTGCATAAATAGATGCCAGGATCTTTTTTTTTAAGTATTAATTACTTAAAA
  AAAGGCTTAAGTCTTTCACGTATTTAGAGAGCTCTGGAAGGCTTTACCCAGTTGACATAC
  CGTGGTAGTAAAAATATTAAATATGGCACACACAAGCCCTGCAACTTGACACTGGCTCAC
  TGAAACAAACATTGAATTCCTAAATCCCAACCATCCAAAGATGTTTATTTTTTATAGAAA
  GATCTGTAAACAAAATATAAATTTCTCAACCGCTTTTACTTTCATAGAAAAAACTTCTAC
  AGAAGGTGACTAACACTTTAAATGTACCATCATTAATAGGGCGGGACACCTTTGAATTTC
  TTGCCCTGAAAAATGCAAACATTTTAATTTAAATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016516
• ORF Size: 2934 bp
• Insert Size: 4640
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_016516.2, NP_057600.2
• RefSeq Size: 3994
• RefSeq ORF: 2934
• Locus ID: 51542
• Gene Summary: This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longer isoform (1).