ATF3 (NM_001674) Human Untagged Clone

CAT#: SC108959

ATF3 (untagged)-Human activating transcription factor 3 (ATF3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATF3
• Synonyms: FLJ41705
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001674 edited
  GAATTCGGCACGAGGGCGCACTGCACAGCTCTCTTCTCTCGCCGCCGCCCGAGCGCACCC
  TTCAGCCCGCGCGCCGGCCGTGAGTCCTCGGTGCTCGCCCGCCGGCCAGACAAACAGCCC
  GCCCGACCCCGTCCCGACCCTGGCCGCCCCGAGCGGAGCCTGGAGCAAAATGATGCTTCA
  ACACCCAGGCCAGGTCTCTGCCTCGGAAGTGAGTGCTTCTGCCATCGTCCCCTGCCTGTC
  CCCTCCTGGGTCACTGGTGTTTGAGGATTTTGCTAACCTGACGCCCTTTGTCAAGGAAGA
  GCTGAGGTTTGCCATCCAGAACAAGCACCTCTGCCACCGGATGTCCTCTGCGCTGGAATC
  AGTCACTGTCAGCGACAGACCCCTCGGGGTGTCCATCACAAAAGCCGAGGTAGCCCCTGA
  AGAAGATGAAAGGAAAAAGAGGCGACGAGAAAGAAATAAGATTGCAGCTGCAAAGTGCCG
  AAACAAGAAGAAGGAGAAGACGGAGTGCCTGCAGAAAGAGTCGGAGAAGCTGGAAAGTGT
  GAATGCTGAACTGAAGGCTCAGATTGAGGAGCTCAAGAACGAGAAGCAGCATTTGATATA
  CATGCTCAACCTTCATCGGCCCACGTGTATTGTCCGGGCTCAGAATGGGAGGACTCCAGA
  AGATGAGAGAAACCTCTTTATCCAACAGATAAAAGAAGGAACATTGCAGAGCTAAGCAGT
  CGTGGTATGGGGGCGACTGGGGAGTCCTCATTGAATCCTCATTTTATACCCAAAACCCTG
  AAGCCATTGGAGAGCTGTCTTCCTGTGTACCTCTAGAATCCCAGCAGCAGAGAACCATCA
  AGGCGGGAGGGCCTGCAGTGATTCAGCAGGCCCTTCCCATTCTGCCCCAGAGTGGGTCTT
  GGACCAGGGCAAGTGCATCTTTGCCTCAACTCCAGGATTTAGGCCTTAACACACTGGCCA
  TTCTTATGTTCCAGATGGCCCCCAGCTGGTGTCCTGCCCGCCTTTCATCTGGATTCTACA
  AAAAACCAGGATGCCCACCGTTAGGATTCAGGCAGCAGTGTCTGTACCTCGGGTGGGAGG
  GATGGGGCCATCTCCTTCACCGTGGCTACCATTGTCACTCGTAGGGGATGTGGAGTGAGA
  ACAGCATTTAGTGAAGTTGTGCAACGGCCAGGGTTGTGCTTTCTAGCAAATATGCTGTTA
  TGTCCAGAAATTGTGTGTGCAAGAAAACTAGGCAATGTACTCTTCCGATGTTTGTGTCAC
  ACAACACTGATGTGACTTTTATATGCTTTTTCTCAGATCTGGTTTCTAAGAGTTTTGGGG
  GGCGGGGCTGTCACCACGTGCAGTATCTCAAGATATTCAGGTGGCCAGAAGAGCTTGTCA
  GCAAGAGGAGGACAGAATTCTCCCAGCGTTAACACAAAATCCATGGGCAGCATGATGGCA
  GGTCCTCTGTTGCAAACTCAGTTCCAAAGTCACAGGAAGAAAGCAGAAAGTTCAACTTCC
  AAAGGGTTAGGACTCTCCACTCAATGTCTTAGGTCAGGAGTTGTGTCTAGGCTGGAAGAG
  CCAAAGAATATTCCATTTTCCTTTCCTTGTGGTTGAAAACCACAGTCAGTGGAGAGATGT
  TTGGAAACCACAGTCAGTGGAGCCTGGGTGGTACCCAGGCTTTAGCATTATTGGATGTCA
  ATAGCATTGTTTTTGTCATGTAGCTGTTTTAAGAAATCTGGCCCAGGGTGTTTGCAGCTG
  TGAGAAGTCACTCACACTGGCCACAAGGACGCTGGCTACTGTCTATTAAAATTCTGATGT
  TTCTGTGAAATTCTCAGAGTGTTTAATTGTACTCAATGGTATCATTACAATTTTCTGTAA
  GAGAAAATATTACTTATTTATCCTAGTATTCCTAACCTGTCAGAATAATAAATATTGGAA
  CCAAGACATGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001674 unedited
  NNGGAACCGTTCAAAATTTGTAAACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGG
  GCGCACTGCACAGCTCTCTTCTCTCGCCGCCGCCCGAGCGCACCCTTCAGCCCGCGCGCC
  GGCCGTGAGTCCTCGGTGCTCGCCCGCCGGCCAGACAAACAGCCCGCCCGACCCCGTCCC
  GACCCTGGCCGCCCCGAGCGGAGCCTGGAGCAAAATGATGCTTCAACACCCAGGCCAGGT
  CTCTGCCTCGGAAGTGAGTGCTTCTGCCATCGTCCCCTGCCTGTCCCCTCCTGGGTCACT
  GGTGTTTGAGGATTTTGCTAACCTGACGCCCTTTGTCAAGGAAGAGCTGAGGTTTGCCAT
  CCAGAACAAGCACCTCTGCCACCGGATGTCCTCTGCGCTGGAATCAGTCACTGTCAGCGA
  CAGACCCCTCGGGGTGTCCATCACAAAAGCCGAGGTAGCCCCTGAAGAAGATGAAAGGAA
  AAAGAGGCGACGAGAAAGAAATAAGATTGCAGCTGCAAAGTGCCGAAACAAGAAGAAGGA
  GAAGACGGAGTGCCTGCAGAAAGAGTCGGAGAAGCTGGAAAGTGTGAATGCTGAACTGAA
  GGCTCAGATTGAGGAGCTCAAGAACGAGAAGCAGCATTTGATATACATGCTCAACCTTCA
  TCGGCCCACGTGTATTGTCCGGGCTCAGAATGGGAGGACTCCAGAAGATGAGAGAAACCT
  CTTTATCCAACAGATAAAAGAAGGAACATTGCAGAGCTAAGCAGTCGTGGTATGGGGGCG
  ACTGGGGAGTCCTCATTGAATCCTCATTTTATACCCAAAACCCTGAAGCCATTGGAGAGC
  TGTCTTCCTGTGTACCTCTAGAATCCCGCAGCAGAGACCTAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001674 unedited
  TGTATAAAAAAACCGTTTTTTAGAGAAGAGAAATAAAATATTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTAACAATGACTGGGCTCCAATATTTATTATTCG
  GACGGGTTAGGAATACTAGGATAAATAAGTAATTTTTTCTTTTACAAAAAATGGCAAGGA
  TACCATTGAGCACAATTAAACCCTCTGAGAATTCCCCAGAAACATCCAATTTTTAATAAA
  CAGTATCCAGCGCCCTTGGGGCCAGGGGGAGGGACTTACAAAAGGTGAAAACACCCTGGG
  CCAGATTTCTCAAAACAGCTCCCCGACTACCCCCCGGCTTTGGCAACCCCAATAATGCTA
  AAACTGGAGACCCACCTCGCCACCTCTGACTGGGACACACCCCAAAACACCACAAACGCC
  TGAGACCACCCCCTTCACGACAAGGACCTCGTCCCCCCCCCTACCCATACCCACCCCCCC
  ATCTCTCCCTTCCTTATCTCACCCCTCCCCCCCTCCCCCCCTCCCTCCCTACACCTTCCC
  CACCCCACTCTGCCCCACCCCCCTTTGTCTGCTCCACCGCCATCCCTTTTACACATCCCC
  CCCCACTTTCACTCCCACCCGCACTCTTCCTCTCCAATCACCTCTCTCACCGCCTCTCTT
  CTCCCCCCCCCCCCCCCCCCCCGCCACCTCCCCCTCCTCCCCCTTTCTCTCTCCTTCCTC
  CCCCCCTCCCTCTTCTTACCCCCTTCCATGTCTCCCCCCACCACCCTCCACTTCCTCACC
  CGCCCCCTTTTTTCCCCCGCTCTCTGTATCCCCTGATAATCTCCCATTTTTTCTATTCAC
  CCTCACTATCCACCCATTCCACCCCACGCCCTCTTATTATATCTACTCCCTCATCTCTCC
  ACTCCTCTCCTCCCTCCCTCTACGTCCGCACGTACTCACCACACTCTACACTGTTCTCAC
  ATACTACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001674
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001674.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001674.2, NP_001665.1
• RefSeq Size: 2049 bp
• RefSeq ORF: 546 bp
• Locus ID: 467
• Cytogenetics: 1q32.3
• Domains: BRLZ
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]'
  Transcript Variant: This variant (1) encodes the longest isoform (1), which represses rather than activates transcription from promoters with ATF-binding elements. Both variants 1 and 3 encode isoform 1.