BAD (NM_032989) Human Untagged Clone

CAT#: SC108970

BAD (untagged)-Human BCL2-associated agonist of cell death (BAD), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BAD
• Synonyms: BBC2; BCL2L8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032989 edited
  GAATTCGGCACGAGGGGCAGGCCCGGGTCAGGGGCCTCGAGATCGGGCTTGGGCCCAGAG
  CATGTTCCAGATCCCAGAGTTTGAGCCGAGTGAGCAGGAAGACTCCAGCTCTGCAGAGAG
  GGGCCTGGGCCCCAGCCCCGCAGGGGACGGGCCCTCAGGCTCCGGCAAGCATCATCGCCA
  GGCCCCAGGCCTCCTGTGGGACGCCAGTCACCAGCAGGAGCAGCCAACCAGCAGCAGCCA
  TCATGGAGGCGCTGGGGCTGTGGAGATCCGGAGTCGCCACAGCTCCTACCCCGCGGGGAC
  GGAGGACGACGAAGGGATGGGGGAGGAGCCCAGCCCCTTTCGGGGCCGCTCGCGCTCGGC
  GCCCCCCAACCTCTGGGCAGCACAGCGCTATGGCCGCGAGCTCCGGAGGATGAGTGACGA
  GTTTGTGGACTCCTTTAAGAAGGGACTTCCTCGCCCGAAGAGCGCGGGCACAGCAACGCA
  GATGCGGCAAAGCTCCAGCTGGACGCGAGTCTTCCAGTCCTGGTGGGATCGGAACTTGGG
  CAGGGGAAGCTCCGCCCCCTCCCAGTGACCTTCGCTCCACATCCCGAAACTCCACCCGTT
  CCCACTGCCCTGGGCAGCCATCTTGAATATGGGCGGAAGTACTTCCCTCAGGCCTATGCA
  AAAAGAGGATCCGTGCTGTCTCCTTTGGAGGGAGGGCTGACCCAGATTCCCTTCCGGTGC
  GTGTGAAGCCACGGAAGGCTTGGTCCCATCGGAAGTTTTGGGTTTTCCGCCCACAGCCGC
  CGGAAGTGGCTCCGTGGCCCCGCCCTCAGGCTCCGGGCTTTCCCCCAGGCGCCTGCGCTA
  AGTCGCGAGCCAGGTTTAACCGTTGCGTCACCGGGACCCGAGCCCCCGCGATGCCCTGGG
  GGCCGTGCTCACTACCAAATGTTAATAAAGCCCGCGTCTGTGCCGCCAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032989 unedited
  GGTCANAATATGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGGCAGGCCC
  GGGTCAGGGGCCTCGAGATCGGGCTTGGGCCCAGAGCATGTTCCAGATCCCAGAGTTTGA
  GCCGAGTGAGCAGGAAGACTCCAGCTCTGCAGAGAGGGGCCTGGGCCCCAGCCCCGCAGG
  GGACGGGCCCTCAGGCTCCGGCAAGCATCATCGCCAGGCCCCAGGCCTCCTGTGGGACGC
  CAGTCACCAGCAGGAGCAGCCAACCAGCAGCAGCCATCATGGAGGCGCTGGGGCTGTGGA
  GATCCGGAGTCGCCACAGCTCCTACCCCGCGGGGACGGAGGACGACGAAGGGATGGGGGA
  GGAGCCCAGCCCCTTTCGGGGCCGCTCGCGCTCGGCGCCCCCCAACCTCTGGGCAGCACA
  GCGCTATGGCCGCGAGCTCCGGAGGATGAGTGACGAGTTTGTGGACTCCTTTAAGAAGGG
  ACTTCCTCGCCCGAAGAGCGCGGGCACAGCAACGCAGATGCGGCAAAGCTCCAGCTGGAC
  GCGAGTCTTCCAGTCCTGGTGGGATCGGAACTTGGGCAGGGGAAGCTCCGCCCCCTCCCA
  GTGACCTTCGCTCCACATCCCGAAACTCCACCCGTTCCCACTGCCCTGGGCAGCCATCTT
  GAATATGGGCGGAAGTACTTCCCTCAGGCCTATGCAAAAAGAGGATCCGTGCTGTCTCCT
  TTGGAGGGAGGGCTGACCCAGATTCCCTTCCGGTGCGTGTGAAGCCACGGAAGGCTTGGT
  CCCATCGGNAAGTTTGGGTTTTCCGCCCCAGCCGCCGAAGTGGCTNCGTGGCCCCCCCTC
  AGCTCCGGCTTTCCCCCAGCGCTGCGCTAGTCGCGAGCCCAGTTACCGTTGCGTCACGGG
  ACCCGACCCCGCGAGCCCTGGGGCGTGCTACTACCAATGTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032989 unedited
  TTACCGCGGCCGCATTCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTGGGGGGACAAACCCCGGCTTTATTAACATTTGGGAGTGAGCACCGGCCCCAA
  GGCATTGCGGGGGCTCGGGTCCCGGGGACCCAACGGTTAAACCTGGCTTGCGACTTAACG
  CAAGCGCCTGGGGGAAAACCCGGAACCTGAGGGCGGGGCCACGGAGCCACTTTCGGGGGG
  TGGGGGCGGAAAACCCAAAACTTCCGATGGGACCAAACCTTCCGTGGGTTTACACCCCCC
  GGAAGGGAATCTGGGTCAACCCTCCCTTCAAAGGAGACAGCACGGATCCTTTTTTTGCAT
  AAGCCTGAGGGAAATACTTCCGCCCATATTCAAAAAGGCTGCCCAAGGCAATGGGAACGG
  GTGGAGTTTCGGGATGTGGAACCAAAGTCACTGGGAGGGGGCGGAGCTTCCCCTGCCCAA
  GTTCCGATCCCCCCAGGACTGGAAGACTCCCGTCCCGCTGGAGCCTTTCCGCATTTGCGT
  TGTTGTGCCCCCGCTCTTTGGGCGAAGAAGTCCCTTTTTAAAGGAGGCCACAAAATCGTC
  ACTTATTCCTCCGAACCTCCGGGCATATCGCCTTTGTTTCCCCAAGGTGGGGGGCCCCCA
  CCCCCAACCGCCCCAAAGGGGCCTGGCTCTCCCCCCCTCCCTTCCGCTTCCCCTCTCCCC
  CCCGGGCAGAACCTGCGCCAACTTCGATTCCCACTACCCAGGCCCCCATTACGGCCCCTC
  CTGATTGTCCCCCTTCCTGCGCCCGCGCTTCACCGCGGCCCCGCGGCCCCCCATACCTCT
  CTCCTAACCTTAAGGCTCCTCACCCTTGGGTTTTGACCTCCCCCTCTCTTCAACCTAACC
  G
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032989
• Insert Size: 1090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032989.1, NP_116784.1
• RefSeq Size: 956 bp
• RefSeq ORF: 507 bp
• Locus ID: 572
• Cytogenetics: 11q13.1
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Insulin signaling pathway, Melanoma, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, VEGF signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Dec 2019]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein.