Calretinin (CALB2) (NM_001740) Human Untagged Clone

CAT#: SC108994

CALB2 (untagged)-Human calbindin 2 (CALB2), transcript variant CALB2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CALB2
• Synonyms: CAB29; CAL2; CR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108994 sequence for NM_001740 edited (data generated by NextGen Sequencing)
  ATGGCTGGCCCGCAGCAGCAGCCCCCTTACCTGCACCTGGCCGAGCTGACGGCGTCCCAG
  TTCCTGGAAATATGGAAGCACTTTGACGCAGACGGAAATGGGTATATTGAAGGTAAAGAG
  CTAGAAAACTTTTTCCAAGAGCTGGAGAAGGCAAGGAAAGGCTCTGGCATGATGTCAAAG
  AGTGACAACTTTGGAGAAAAGATGAAGGAGTTCATGCAGAAGTATGATAAAAACTCAGAT
  GGGAAAATCGAGATGGCAGAGCTGGCGCAGATCCTGCCAACCGAAGAGAACTTCCTTCTG
  TGCTTCAGGCAGCACGTGGGCTCCAGCGCCGAGTTTATGGAGGCTYGGCGGAAGTACGAC
  ACAGACAGGAGTGGCTACATCGAAGCCAATGAGCTCAAGGGATTCCTGTCAGACCTGCTG
  AAGAAGGCGAACCGGCCGTACGATGAGCCCAAGCTCCAGGAATACACCCAAACCATACTA
  CGGATGTTTGACTTGAACGGGGATGGCAAATTGGGCCTCTCAGAGATGTCCCGACTCCTG
  CCTGTCCAGGAAAACTTCCTGCTTAAATTTCAGGGCATGAAGCTGACCTCAGAGGAGTTT
  AACGCGATCTTCACATTTTACGACAAGGATAGAAGCGGCTACATTGACGAGCATGAGCTG
  GATGCCCTTTTGAAGGATCTGTACGAGAAAAACAAAAAGGAAATGAATATTCAACAGCTC
  ACCAACTACAGAAAGAGCGTCATGTCCTTGGCAGAGGCAGGGAAGCTCTACCGCAAGGAC
  CTGGAGATTGTGCTCTGCAGCGAGCCCCCCATGTAA
  
  Clone variation with respect to NM_001740.4
  346 t=>y
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001740 unedited
  GTCAGGATATGTATACGACTCCTATAGGCGGCCGCGCAATTCGCACGAGGGCGGAGCGGG
  AGCGGTGCAGGCTGAGGTCTCCGAGCGGCTCGCCATGGCTGGCCCGCAGCAGCAGCCCCC
  TTACCTGCACCTGGCCGAGCTGACGGCGTCCCAGTTCCTGGAAATATGGAAGCACTTTGA
  CGCAGACGGAAATGGGTATATTGAAGGTAAAGAGCTAGAAAACTTTTTCCAAGAGCTGGA
  GAAGGCAAGGAAAGGCTCTGGCATGATGTCAAAGAGTGACAACTTTGGAGAAAAGATGAA
  GGAGTTCATGCAGAAGTATGATAAAAACTCAGATGGGAAAATCGAGATGGCAGAGCTGGC
  GCAGATCCTGCCAACCGAAGAGAACTTCCTTCTGTGCTTCAGGCAGCACGTGGGCTCCAG
  CGCCGAGTTTATGGAGGCTTGGCGGAAGTACGACACAGACAGGAGTGGCTACATCGAAGC
  CAATGAGCTCAAGGGATTCCTGTCAGACCTGCTGAAGAAGGCGAACCGGCCGTACGATGA
  GCCCAAGCTCCAGGAATACACCCAAACCATACTACGGATGTTTGACTTGAACGGGGATGG
  CAAATTGGGCCTCTCAGAGATGTCCCGACTCCTGCCTGTCCAGGAAAACTTCCTGCTTAA
  ATTTCAGGGCATGAAGCTGACCTCAGAGGAGTTTAACGCGATCTTCACATTTTACGACAA
  GGATAGAAGCGGCTACATTGACGAGCATGAGCTGGATGCCCTTTTGAAGGATCTGTACGA
  GAAAAACANAAAGGAATGAATATTCAACAGCTCACCAACTACAGAAAGAGCGTCATGTCC
  TTGGNCAGAGCACGGNAAGCTCTACCGCAAGGACCTGGAGTATGTGCTCTGCAGCGAGCC
  CCCATGTAAGTGGGGGACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001740 unedited
  CGCGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTTCCACAGCAGGACCTTTCCATTTTATTG
  GCTGGAAACTTTTTTTTGGGAAACAAAAAACCCAAAAAAAAAAGGGCCCCCCCCAGTAAA
  AAAGGCAAGGAAAGGGCGGGTGCCTTCCACACACAGGAGTTTTGAAAGGACACCTCCCCC
  ATTGGCAAGGGGGGGGATGGAACCTGAAAATTTGGGGGGGGTGGGGGCATGGGGGACCCG
  CAAAGGGGACCCAAAGGGAAAAATCCCCTTTGTTGGACATAAAACCCTAAATCATACAGC
  GAGGGAGCTCATGCCTCACAGCGAAAAAAATTAGGGACCCTCCACCCATGCCCCCATAAC
  CTTTCATGTGGGGGAACCACTGAACAAAAACTTCCTGGGCAGGGGTGGGTGCAAGGGGCT
  TAAGACAACCCCCCAATTGCCCATCCTTTATTTTTTGTTTCCTGCTTTTCAGGCAAGTGT
  GTGCAAGCTTTAAGGGTGGGGGCGGGGCCCTCACGGTCTTTGAGTCTGGGTAAACCCATC
  AGGGCAACAAAACCAGGGTTTGGGGGAGGGGGAAAAACAACCCCCGTCCCCACTTTAATG
  GGGGGCTCGCTGCAAACACAATTTCCAGGTCCTTGCGGGAAAACTTCCCTGCCTCTGCCA
  AGGACATGACACTTTTTTTGTAANTGGGGGAGCCGGGAGAAATTCATTTCCCTTTTGGTT
  TTCTCGAACAGAACCTTCAAAGGGCATTCAGCTCATGCTTGNAAATGANCCGCTTTTATC
  CTTGTCGAAAAAGGGAAGATCCCGTAAACTCCTTGGGTCCACTTATGCCCGAAATTAACA
  GGAAGTTCCTGGAAGGCAGAGTCGGGATCTTGA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001740
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001740.2, NP_001731.1
• RefSeq Size: 1453 bp
• RefSeq ORF: 816 bp
• Locus ID: 794
• Cytogenetics: 16q22.2
• Domains: EFh
• Gene Summary: 'This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (1) encodes the longer isoform (isoform 1), which has also been designated as the full-length isoform.