Calretinin (CALB2) (NM_001740) Human Untagged Clone

CAT#: SC324074

CALB2 (untagged)-Human calbindin 2 (CALB2), transcript variant CALB2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CALB2
• Synonyms: CAB29; CAL2; CR
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001740.2 GCCCAGCCGGCGCGGAGCGGGAGCGGTGCAGGCTGAGGTCTCCGAGCGGCTCGCCATGGC
  TGGCCCGCAGCAGCAGCCCCCTTACCTGCACCTGGCCGAGCTGACGGCGTCCCAGTTCCT
  GGAAATATGGAAGCACTTTGACGCAGACGGAAATGGGTATATTGAAGGTAAAGAGCTAGA
  AAACTTTTTCCAAGAGCTGGAGAAGGCAAGGAAAGGCTCTGGCATGATGTCAAAGAGTGA
  CAACTTTGGAGAAAAGATGAAGGAGTTCATGCAGAAGTATGATAAAAACTCAGATGGGAA
  AATCGAGATGGCAGAGCTGGCGCAGATCCTGCCAACCGAAGAGAACTTCCTTCTGTGCTT
  CAGGCAGCACGTGGGCTCCAGCACCGAGTTTATGGAGGCTTGGCGGAAGTACGACACAGA
  CAGGAGTGGCTACATCGAAGCCAATGAGCTCAAGGGATTCCTGTCAGACCTGCTGAAGAA
  GGCGAACCGGCCGTACGATGAGCCCAAGCTCCAGGAATACACCCAAACCATACTACGGAT
  GTTTGACTTGAACGGGGATGGCAAATTGGGCCTCTCAGAGATGTCCCGACTCCTGCCTGT
  CCAGGAAAACTTCCTGCTTAAATTTCAGGGCATGAAGCTGACCTCAGAGGAGTTTAACGC
  GATCTTCACATTTTACGACAAGGATAGAAGCGGCTACATTGACGAGCATGAGCTGGATGC
  CCTTTTGAAGGATCTGTACGAGAAAAACAAAAAGGAAATGAATATTCAACAGCTCACCAA
  CTACAGAAAGAGCGTCATGTCCTTGGCAGAGGCAGGGAAGCTCTACCGCAAGGACCTGGA
  GATTGTGCTCTGCAGCGAGCCCCCCATGTAAAGTGGGGACGGGGGCTGCTTCTCCACCTC
  CCCCAAACCCTGCTTCTGCTGCCCTGATGCGTCTACCCAGACTCAGAGACCGTGAGCGCC
  CCGCCCCCACCCCTACAGCCTGCACACACCTGCCTGCAGAGCAGGAAACGAGAGATAGAG
  GATGGGCAGCTGGGGGGCTGTCCTGAGCCCCCTGCACCCACCCCTGCCCAGGCAGTCTTT
  GCTCAGTGGATCACACACATGGAAGGTGATGGGGGCATGGGTGGAGGGTCCCTAATTCTC
  TTCGCTGTGATGCATGAGCTCCCTCGCTGTATGATTTAGGCTTCTATGTCCAACAGAGTG
  GACTCTTCCCTCTCGCTCCCCTCTGCCGGTCCCCCATGCCACCACCCACCCCAAACTTCC
  AGGTTCCATCCACCACCTTGCCAATGGTGTAGCTGTCCTCTCAGAACTCCTGTGTGTGGA
  AGGCACCCGCCCTTTCCTTGCCTTCTTTACTCGGCGTGCTCCTTTTCTCTTTGGGTTTCT
  TGTTTACCAAAGAAGAGTTTACAGACAATAAAATGGAAAGGTCCTGCTGTGGAAACTTAA
  AAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001740
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001740.2, NP_001731.1
• RefSeq Size: 1453 bp
• RefSeq ORF: 816 bp
• Locus ID: 794
• Cytogenetics: 16q22.2
• Domains: EFh
• Gene Summary: 'This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (1) encodes the longer isoform (isoform 1), which has also been designated as the full-length isoform.