CD97 (ADGRE5) (NM_001784) Human Untagged Clone

CAT#: SC109039

CD97 (untagged)-ORIGENE UNIQUE VARIANT 1 of Human CD97 molecule (CD97)


  "NM_001784" in other vectors (7)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ADGRE5
Synonyms CD97; TM7LN1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_001784, the custom clone sequence may differ by one or more nucleotides


ATGGGAGGCCGCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCA
GGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTGCAATCCAGG
GTTCAGCTCTTTTTCTGAGATCATCACCACCCCGACGGAGACTTGTGACGACATCAACGAGTGTGCAACA
CCGTCGAAAGTGTCATGCGGAAAATTCTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCA
GCCCGGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGT
GGACGAGTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATAC
AGCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGACACTGTCTGTG
AAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCGATTCTTCGA
CAAAGTCCAGGACCTGGGCAGAGACTCCAAGACAAGCTCAGCCGAGGTCACCATCCAGAATGTCATCAAA
TTGGTGGATGAACTGATGGAAGCTCCTGGAGACGTAGAGGCCCTGGCGCCACCTGTCCGGCACCTCATAG
CCACCCAGCTGCTCTCAAACCTTGAAGATATCATGAGGATCCTGGCCAAGAGCCTGCCTAAAGGCCCCTT
CACCTACATTTCCCCTTCGAACACAGAGCTGACCCTGATGATCCAGGAGCGGGGGGACAAGAACGTCACT
ATGGGTCAGAGCAGCGCACGCATGAAGCTGAATTGGGCTGTGGCAGCTGGAGCCGAGGATCCAGGCCCCG
CCGTGGCGGGCATCCTCTCCATCCAGAACATGACGACATTGCTGGCCAATGCCTCCTTGAACCTGCATTC
CAAGAAGCAAGCCGAACTGGAGGAGATATATGAAAGCAGCATCCGTGGTGTCCAACTCAGACGCCTCTCT
GCCGTCAACTCCATCTTTCTGAGCCACAACAACACCAAGGAACTCAACTCCCCCATCCTTTTCGCCTTCT
CCCACCTTGAGTCCTCCGATGGGGAGGCGGGAAGAGACCCTCCTGCCAAGGACGTGATGCCTGGGCCACG
GCAGGAGCTGCTCTGTGCCTTCTGGAAGAGTGACAGCGACAGGGGAGGGCACTGGGCCACCGAGGGCTGC
CAGGTGCTGGGCAGCAAGAACGGCAGCACCACCTGCCAATGCAGCCACCTGAGCAGCTTTGCGATCCTTA
TGGCTCATTATGACGTGGAGGACTGGAAGCTGACCCTGATCACCAGGGTGGGACTGGCGCTGTCACTCTT
CTGCCTGCTGCTGTGCATCCTCACTTTCCTGCTGGTGCGGCCCATCCAGGGCTCGCGCACCACCATACAC
CTGCACCTCTGCATCTGCCTCTTCGTGGGCTCCACCATCTTCCTGGCCGGCATCGAGAACGAAGGCGGCC
AGGTGGGGCTGCGCTGCCGCCTGGTGGCCGGGCTGCTGCACTACTGTTTCCTGGCCGCCTTCTGCTGGAT
GAGCCTCGAAGGCCTGGAGCTCTACTTTCTTGTGGTGCGCGTGTTCCAAGGCCAGGGCCTGAGTACGCGC
TGGCTCTGCCTGATCGGCTATGGCGTGCCCCTGCTCATCGTGGGCGTCTCGGCTGCCATCTACAGCAAGG
GCTACGGCCGCCCCAGATACTGCTGGTTGGACTTTGAGCAGGGCTTCCTCTGGAGCTTCTTGGGACCTGT
GACCTTCATCATTTTGTGCAATGCTGTCATTTTCGTGACTACCGTCTGGAAGCTCACTCAGAAGTTTTCT
GAAATCAATCCAGACATGAAGAAATTAAAGAAGGCGAGGGCGCTGACCATCACGGCCATCGCGCAGCTCT
TCCTGTTGGGCTGCACCTGGGTCTTTGGCCTGTTCATCTTCGACGATCGGAGCTTGGTGCTGACCTATGT
GTTTACCATCCTCAACTGCCTGCAGGGCGCCTTCCTCTACCTGCTGCACTGCCTGCTCAACAAGAAGGTT
CGGGAAGAATACCGGAAGTGGGCCTGCCTAGTTGCTGGGGGGAGCAAGTACTCAGAATTCACCTCCACCA
CGTCTGGCACTGGCCACAATCAGACCCGGGCCCTCAGGGCATCAGAGTCCGGCATATGA


>OriGene 5' read for NM_001784 unedited
GCATCACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGTGGAGACGGGACAG
CCCTGTCCCACTCACTCTTTCCCCTGCCGCTCCTGCCGGCAGCTCCAACCATGGGAGGCC
GCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCA
GGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGCCACCGCCTGTCGCT
GCAATCCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCGACGGAGACTTGTGACG
ACATCAACGAGTGTGCAACACCGTCGAAAGTGTCATGCGGAAAATTCTCGGACTGCTGGA
ACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCGGGATATGAGCCTGTTTCTGGGGCAA
AAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGTGGACGAGTGCAGCTCCGGGC
AGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATACAGCTGCCGCT
GCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGATAACCAAAAGGACACTGTCTGTGA
AGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCG
ATTCTTCGAACAAGTCAGGACCTNGGCAGAGACTCCAAGACCAGCTCAGCCGAGGTCACC
ATCCAGAATGTCATCAAATTGGTGGATGAACTGATGGAAGCTNCTGGAGACGTANAGGCC
CTGCCCCACCTGTCCGCACCTCTAGCCACCAGCTGTCTCAAACCTGAGATTCATGAGGAT
CTGCCCAAACCTGCCTAAGCCCCTTACTACATTCCCTTGAAACANACTGCCCTGAGATCC
AGACGGGGGAAGCACTCCTTT
>OriGene 3' read for NM_001784 unedited
CGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCTGTATGCATGTGTTTAAT
TTTAAGTGTCAACACTGAAAAAATTTTAACAGAATAAAAACACATTACACAACGTTCTAC
AACCTTTCACAACAGTGAGAAACCTCCCTGCCCGGCCAGGCAGGGCAGGCCTTCTGTACC
GTGAGCCTCAGGCAGTGGCCCCAAGGGGCCTCGCCATGGGGCCTCTGACATCAGTCTTAG
CTTAAGAGGGAAGCTGAGCCCCAGCGCCTAGTCTCTTCAGTTCTGCAGCAAAGACATGAG
AGGAAAAGTCCAGGATGGGACAAGCGCCCTTAGTCTGTCCCGAGTACTGGCCACAGGTGC
CAGGGCAACATGCTGCATTGCAGCCCTGGGCCAGCCCCTGTCCCCACCCTGGGTCACAAG
GTGGAGCAGAGAGGCAGCCAGCAGCAGGACCAGTGGCTCCGACTCCACCCCACTGGGTGT
CCTGGACTTTGGTGCCAGACTATAGCTGCCACTCCCTCCTGGTGGCACACGGGATCAGGG
AGGGAGGGTGGAGGGATANAAGTGGTGGACGAAGGAAGGATTGGATGNTCTTNNNNTNCC
AAGCCGTTCGGGCCCCTCCGCTTTGCCGCGTCCTCCCCCNTTTCTCCCCCCTTTCCATTC
TCCTCTTCTTTTCCCTCTTCCGCTCTCCCTATTATATTCTTTTCCCGTCACCCTACTCCG
CCGTCTCCTCTTTTTCTCTTCGTTCACCCTTCTCCCACGCCCCGCCCCCGTCTTCCGCAT
CTCCTTTCCACTCCATTCCTTCGTATCTCCCCTATCCCTCCACTCTCCCACTCCCTTCAC
TCTACTCCTTCCCCCCCTTCCCCCATTCCCCCCCCTTTTCCCTCCCGTTCTTTCCCCTCA
TTATCCCTCCCCTTCCTCCCCCCACTTTTTTTCCTCCCACCCCCATTTCATCTTTCTTTA
TCCCCTTCTCTTTCTCTTTTTCTTTCATTTTCCCTCCATCCTNTTCCCTCTCTTCTCCTC
GTACGTCACTTCTCTCATCCA
Restriction Sites NotI-NotI     
ACCN NM_001784
Insert Size 3000 bp
OTI Disclaimer The sequence of an 'OriGene Unique Variant' differs significantly from the associated reference. It represents a novel splice variant from the same gene locus of the reference. Although such variants are true transcripts and present opportunity for discoveries, they are not yet curated by NCBI and should not be used if the exact reference accession sequence is required.
OTI Annotation This TrueClone was found to represent an alternative form of the specific reference to which it is associated. Its Open Reading Frame (ORF) may represent a novel form or alternative splice variant. By virtue of it being a true transcript (cDNA clone not PCR product), it provides a biologically relevant copy of its mRNA template. For more details, please evaluate the sequence information provided on this website or contact our customer care specialists.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001784.3, NP_001775.2
RefSeq Size 2968 bp
RefSeq ORF 2229 bp
Locus ID 976
Cytogenetics 19p13.12
Domains GPS, 7tm_2, EGF_CA, EGF, EGF
Protein Families Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, GPCR, Secreted Protein, Transmembrane
Gene Summary 'This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]'
Transcript Variant: This variant (2) lacks two consecutive exons in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2, also known as CD97(EGF 1,2,5)) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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