CD97 (ADGRE5) (NM_001784) Human Untagged Clone

CAT#: SC109039

CD97 (untagged)-ORIGENE UNIQUE VARIANT 1 of Human CD97 molecule (CD97)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADGRE5
• Synonyms: CD97; TM7LN1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001784, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGAGGCCGCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCA
  GGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTGCAATCCAGG
  GTTCAGCTCTTTTTCTGAGATCATCACCACCCCGACGGAGACTTGTGACGACATCAACGAGTGTGCAACA
  CCGTCGAAAGTGTCATGCGGAAAATTCTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCA
  GCCCGGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGT
  GGACGAGTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATAC
  AGCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGACACTGTCTGTG
  AAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCGATTCTTCGA
  CAAAGTCCAGGACCTGGGCAGAGACTCCAAGACAAGCTCAGCCGAGGTCACCATCCAGAATGTCATCAAA
  TTGGTGGATGAACTGATGGAAGCTCCTGGAGACGTAGAGGCCCTGGCGCCACCTGTCCGGCACCTCATAG
  CCACCCAGCTGCTCTCAAACCTTGAAGATATCATGAGGATCCTGGCCAAGAGCCTGCCTAAAGGCCCCTT
  CACCTACATTTCCCCTTCGAACACAGAGCTGACCCTGATGATCCAGGAGCGGGGGGACAAGAACGTCACT
  ATGGGTCAGAGCAGCGCACGCATGAAGCTGAATTGGGCTGTGGCAGCTGGAGCCGAGGATCCAGGCCCCG
  CCGTGGCGGGCATCCTCTCCATCCAGAACATGACGACATTGCTGGCCAATGCCTCCTTGAACCTGCATTC
  CAAGAAGCAAGCCGAACTGGAGGAGATATATGAAAGCAGCATCCGTGGTGTCCAACTCAGACGCCTCTCT
  GCCGTCAACTCCATCTTTCTGAGCCACAACAACACCAAGGAACTCAACTCCCCCATCCTTTTCGCCTTCT
  CCCACCTTGAGTCCTCCGATGGGGAGGCGGGAAGAGACCCTCCTGCCAAGGACGTGATGCCTGGGCCACG
  GCAGGAGCTGCTCTGTGCCTTCTGGAAGAGTGACAGCGACAGGGGAGGGCACTGGGCCACCGAGGGCTGC
  CAGGTGCTGGGCAGCAAGAACGGCAGCACCACCTGCCAATGCAGCCACCTGAGCAGCTTTGCGATCCTTA
  TGGCTCATTATGACGTGGAGGACTGGAAGCTGACCCTGATCACCAGGGTGGGACTGGCGCTGTCACTCTT
  CTGCCTGCTGCTGTGCATCCTCACTTTCCTGCTGGTGCGGCCCATCCAGGGCTCGCGCACCACCATACAC
  CTGCACCTCTGCATCTGCCTCTTCGTGGGCTCCACCATCTTCCTGGCCGGCATCGAGAACGAAGGCGGCC
  AGGTGGGGCTGCGCTGCCGCCTGGTGGCCGGGCTGCTGCACTACTGTTTCCTGGCCGCCTTCTGCTGGAT
  GAGCCTCGAAGGCCTGGAGCTCTACTTTCTTGTGGTGCGCGTGTTCCAAGGCCAGGGCCTGAGTACGCGC
  TGGCTCTGCCTGATCGGCTATGGCGTGCCCCTGCTCATCGTGGGCGTCTCGGCTGCCATCTACAGCAAGG
  GCTACGGCCGCCCCAGATACTGCTGGTTGGACTTTGAGCAGGGCTTCCTCTGGAGCTTCTTGGGACCTGT
  GACCTTCATCATTTTGTGCAATGCTGTCATTTTCGTGACTACCGTCTGGAAGCTCACTCAGAAGTTTTCT
  GAAATCAATCCAGACATGAAGAAATTAAAGAAGGCGAGGGCGCTGACCATCACGGCCATCGCGCAGCTCT
  TCCTGTTGGGCTGCACCTGGGTCTTTGGCCTGTTCATCTTCGACGATCGGAGCTTGGTGCTGACCTATGT
  GTTTACCATCCTCAACTGCCTGCAGGGCGCCTTCCTCTACCTGCTGCACTGCCTGCTCAACAAGAAGGTT
  CGGGAAGAATACCGGAAGTGGGCCTGCCTAGTTGCTGGGGGGAGCAAGTACTCAGAATTCACCTCCACCA
  CGTCTGGCACTGGCCACAATCAGACCCGGGCCCTCAGGGCATCAGAGTCCGGCATATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001784 unedited
  GCATCACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGTGGAGACGGGACAG
  CCCTGTCCCACTCACTCTTTCCCCTGCCGCTCCTGCCGGCAGCTCCAACCATGGGAGGCC
  GCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCA
  GGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGCCACCGCCTGTCGCT
  GCAATCCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCGACGGAGACTTGTGACG
  ACATCAACGAGTGTGCAACACCGTCGAAAGTGTCATGCGGAAAATTCTCGGACTGCTGGA
  ACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCGGGATATGAGCCTGTTTCTGGGGCAA
  AAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGTGGACGAGTGCAGCTCCGGGC
  AGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATACAGCTGCCGCT
  GCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGATAACCAAAAGGACACTGTCTGTGA
  AGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCG
  ATTCTTCGAACAAGTCAGGACCTNGGCAGAGACTCCAAGACCAGCTCAGCCGAGGTCACC
  ATCCAGAATGTCATCAAATTGGTGGATGAACTGATGGAAGCTNCTGGAGACGTANAGGCC
  CTGCCCCACCTGTCCGCACCTCTAGCCACCAGCTGTCTCAAACCTGAGATTCATGAGGAT
  CTGCCCAAACCTGCCTAAGCCCCTTACTACATTCCCTTGAAACANACTGCCCTGAGATCC
  AGACGGGGGAAGCACTCCTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001784 unedited
  CGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTCTGTATGCATGTGTTTAAT
  TTTAAGTGTCAACACTGAAAAAATTTTAACAGAATAAAAACACATTACACAACGTTCTAC
  AACCTTTCACAACAGTGAGAAACCTCCCTGCCCGGCCAGGCAGGGCAGGCCTTCTGTACC
  GTGAGCCTCAGGCAGTGGCCCCAAGGGGCCTCGCCATGGGGCCTCTGACATCAGTCTTAG
  CTTAAGAGGGAAGCTGAGCCCCAGCGCCTAGTCTCTTCAGTTCTGCAGCAAAGACATGAG
  AGGAAAAGTCCAGGATGGGACAAGCGCCCTTAGTCTGTCCCGAGTACTGGCCACAGGTGC
  CAGGGCAACATGCTGCATTGCAGCCCTGGGCCAGCCCCTGTCCCCACCCTGGGTCACAAG
  GTGGAGCAGAGAGGCAGCCAGCAGCAGGACCAGTGGCTCCGACTCCACCCCACTGGGTGT
  CCTGGACTTTGGTGCCAGACTATAGCTGCCACTCCCTCCTGGTGGCACACGGGATCAGGG
  AGGGAGGGTGGAGGGATANAAGTGGTGGACGAAGGAAGGATTGGATGNTCTTNNNNTNCC
  AAGCCGTTCGGGCCCCTCCGCTTTGCCGCGTCCTCCCCCNTTTCTCCCCCCTTTCCATTC
  TCCTCTTCTTTTCCCTCTTCCGCTCTCCCTATTATATTCTTTTCCCGTCACCCTACTCCG
  CCGTCTCCTCTTTTTCTCTTCGTTCACCCTTCTCCCACGCCCCGCCCCCGTCTTCCGCAT
  CTCCTTTCCACTCCATTCCTTCGTATCTCCCCTATCCCTCCACTCTCCCACTCCCTTCAC
  TCTACTCCTTCCCCCCCTTCCCCCATTCCCCCCCCTTTTCCCTCCCGTTCTTTCCCCTCA
  TTATCCCTCCCCTTCCTCCCCCCACTTTTTTTCCTCCCACCCCCATTTCATCTTTCTTTA
  TCCCCTTCTCTTTCTCTTTTTCTTTCATTTTCCCTCCATCCTNTTCCCTCTCTTCTCCTC
  GTACGTCACTTCTCTCATCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001784
• Insert Size: 3000 bp
• OTI Disclaimer: The sequence of an 'OriGene Unique Variant' differs significantly from the associated reference. It represents a novel splice variant from the same gene locus of the reference. Although such variants are true transcripts and present opportunity for discoveries, they are not yet curated by NCBI and should not be used if the exact reference accession sequence is required.
• OTI Annotation: This TrueClone was found to represent an alternative form of the specific reference to which it is associated. Its Open Reading Frame (ORF) may represent a novel form or alternative splice variant. By virtue of it being a true transcript (cDNA clone not PCR product), it provides a biologically relevant copy of its mRNA template. For more details, please evaluate the sequence information provided on this website or contact our customer care specialists.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001784.3, NP_001775.2
• RefSeq Size: 2968 bp
• RefSeq ORF: 2229 bp
• Locus ID: 976
• Cytogenetics: 19p13.12
• Domains: GPS, 7tm_2, EGF_CA, EGF, EGF
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, GPCR, Secreted Protein, Transmembrane
• Gene Summary: 'This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]'
  Transcript Variant: This variant (2) lacks two consecutive exons in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2, also known as CD97(EGF 1,2,5)) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.