CD97 (ADGRE5) (NM_001784) Human Untagged Clone

CAT#: SC109040

CD97 (untagged)-Human CD97 molecule (CD97), transcript variant 2


  "NM_001784" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ADGRE5
Synonyms CD97; TM7LN1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC109040 sequence for NM_001784 edited (data generated by NextGen Sequencing)
ATGGGAGGCCGCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACC
CAGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGCCACC
GCCTGTCGCTGCAATCCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCGACGGAG
ACTTGTGACGACATCAACGAGTGTGCAACACCGTCGAAAGTGTCATGCGGAAAATTCTCG
GACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCGGGATATGAGCCTGTT
TCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGTGGACGAGTGC
AGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATAC
AGCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGAC
ACTGTCTGTGAAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAG
ACGCTTTCCCGATTCTTCGACAAAGTCCAGGACCTGGGCAGAGACTCCAAGACAAGCTCA
GCCGAGGTCACCATCCAGAATGTCATCAAATTGGTGGATGAACTGATGGAAGCTCCTGGA
GACGTAGAGGCCCTGGCGCCACCTGTCCGGCACCTCATAGCCACCCAGCTGCTCTCAAAC
CTTGAAGATATCATGAGGATCCTGGCCAAGAGCCTGCCTAAAGGCCCCTTCACCTACATT
TCCCCTTCGAACACAGAGCTGACCCTGATGATCCAGGAGCGGGGGGACAAGAACGTCACT
ATGGGTCAGAGCAGCGCACGCATGAAGCTGAATTGGGCTGTGGCAGCTGGAGCCGAGGAT
CCAGGCCCCGCCGTGGCGGGCATCCTCTCCATCCAGAACATGACGACATTGCTGGCCAAT
GCCTCCTTGAACCTGCATTCCAAGAAGCAAGCCGAACTGGAGGAGATATATGAAAGCAGC
ATCCGTGGTGTCCAACTCAGACGCCTCTCTGCCGTCAACTCCATCTTTCTGAGCCACAAC
AACACCAAGGAACTCAACTCCCCCATCCTTTTCGCCTTCTCCCACCTTGAGTCCTCCGAT
GGGGAGGCGGGAAGAGACCCTCCTGCCAAGGACGTGATGCCTGGGCCACGGCAGGAGCTG
CTCTGTGCCTTCTGGAAGAGTGACAGCGACAGGGGAGGGCACTGGGCCACCGAGGGCTGC
CAGGTGCTGGGCAGCAAGAACGGCAGCACCACCTGCCAATGCAGCCACCTGAGCAGCTTT
GCGATCCTTATGGCTCATTATGACGTGGAGGACTGGAAGCTGACCCTGATCACCAGGGTG
GGACTGGCGCTGTCACTCTTCTGCCTGCTGCTGTGCATCCTCACTTTCCTGCTGGTGCGG
CCCATCCAGGGCTCGCGCACCACCATACACCTGCACCTCTGCATCTGCCTCTTCGTGGGC
TCCACCATCTTCCTGGCCGGCATCGAGAACGAAGGCGGCCAGGTGGGGCTGCGCTGCCGC
CTGGTGGCCGGGCTGCTGCACTACTGTTTCCTGGCCGCCTTCTGCTGGATGAGCCTCGAA
GGCCTGGAGCTCTACTTTCTTGTGGTGCGCGTGTTCCAAGGCCAGGGCCTGAGTACGCGC
TGGCTCTGCCTGATCGGCTATGGCGTGCCCCTGCTCATCGTGGGCGTCTCGGCTGCCATC
TACAGCAAGGGCTACGGCCGCCCCAGATACTGCTGGTTGGACTTTGAGCAGGGCTTCCTC
TGGAGCTTCTTGGGACCTGTGACCTTCATCATTTTGTGCAATGCTGTCATTTTCGTGACT
ACCGTCTGGAAGCTCACTCAGAAGTTTTCTGAAATCAATCCAGACATGAAGAAATTAAAG
AAGGCGAGGGCGCTGACCATCACGGCCATCGCGCAGCTCTTCCTGTTGGGCTGCACCTGG
GTCTTTGGCCTGTTCATCTTCGACGATCGGAGCTTGGTGCTGACCTATGTGTTTACCATC
CTCAACTGCCTGCAGGGCGCCTTCCTCTACCTGCTGCACTGCCTGCTCAACAAGAAGGTT
CGGGAAGAATACCGGAAGTGGGCCTGCCTAGTTGCTGGGGGGAGCAAGTACTCAGAATTC
ACCTCCACCACGTCTGGCACTGGCCACAATCAGACCCGGGCCCTCAGGGCATCAGAGTCC
GGCAYATGA

Clone variation with respect to NM_001784.4
2225 t=>y
>OriGene 5' read for NM_001784 unedited
GAACCGTCAAATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGAGACG
GGACAGCCCTGTCCCACTCACTCTTTCCCCTGCCGCTCCTGCCGGCAGCTCCAACCATGG
GAGGCCGCGTCTTTCTCGCATTCTGTGTCTGGCTGACTCTGCCGGGAGCTGAAACCCAGG
ACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGAACTCCTCGTGTGTCAATGCCACCGCCT
GTCGCTGCAATCCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCGACGGAGACTT
GTGACGACATCAACGAGTGTGACACACCGTCGAAAGTGTCATGCGGAAAATTCTCGGACT
GCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCGGGATATGAGCCTGTTTCTG
GGGCAAAAACATTCAAGAATGAGAGCGAGAACACCTGTCAAGATGTGGACGAGTGCAGCT
CCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATACAGCT
GCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGACACTG
TCTGTGAAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGC
TTTCCCGATTCTTCGACAAAGTCCAGGACCTGGGCAGAGACTCCAAGACAAGCTCAGCCG
AGGTCACCATCCAGAATGGTCATCAATTGGGTGGATGAACTGATGGAAGCTCCTGGAAAC
GTATAGGCCCTGGCGCCACCTGTCCGGCACCTTATAGCCCCCAAGCTGCTCTCAAACCTT
TGAGAAATCATGAGGATCCCGGCCAAGAACCTGCTTAAGGCCCCCTCCCC
Restriction Sites NotI-NotI     
ACCN NM_001784
Insert Size 3000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001784.3, NP_001775.2
RefSeq Size 2968 bp
RefSeq ORF 2229 bp
Locus ID 976
Cytogenetics 19p13.12
Domains GPS, 7tm_2, EGF_CA, EGF, EGF
Protein Families Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, GPCR, Secreted Protein, Transmembrane
Gene Summary 'This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19. [provided by RefSeq, Jun 2011]'
Transcript Variant: This variant (2) lacks two consecutive exons in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2, also known as CD97(EGF 1,2,5)) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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