COMT (NM_007310) Human Untagged Clone

CAT#: SC109094

COMT (untagged)-Human catechol-O-methyltransferase (COMT), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COMT
• Synonyms: HEL-S-98n
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109094 sequence for NM_007310 edited (data generated by NextGen Sequencing)
  ATGGGTGACACCAAGGAGCAGCGCATCCTGAACCACGTGCTGCAGCATGCGGAGCCCGGG
  AACGCACAGAGCGTGCTGGAGGCCATTGACACCTACTGCGAGCAGAAGGAGTGGGCCATG
  AACGTGGGCGACAAGAAAGGCAAGATCGTGGACGCCGTGATTCAGGAGCACCAGCCCTCC
  GTGCTGCTGGAGCTGGGGGCCTACTGTGGCTACTCAGCTGTGCGCATGGCCCGCCTGCTG
  TCACCAGGGGCGAGGCTGATCACCATCGAGATCAACCCCGACTGTGCCGCCATCACCCAG
  CGGATGGTGGATTTCGCTGGCGTGAAGGACAAGGTCACCCTTGTGGTTGGAGCGTCCCAG
  GACATCATCCCCCAGCTGAAGAAGAAGTATGATGTGGACACACTGGACATGGTCTTCCTC
  GACCACTGGAAGGACCGGTACCTGCCGGACACGCTTCTCTTGGAGGAATGTGGCCTGCTG
  CGGAAGGGGACAGTGCTACTGGCTGACAACGTGATCTGCCCAGGTGCGCCAGACTTCCTA
  GCACACGTGCGCGGGAGCAGCTGCTTTGAGTGCACACACTACCAATCGTTCCTGGAATAC
  AGGGAGGTGGTGGACGGCCTGGAGAAGGCCATCTACAAGGGCCCAGGCAGCGAAGCAGGG
  CCCTGA
  
  Clone variation with respect to NM_007310.2
  258 c=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007310 unedited
  TATTTTGTAATACGACTCACTATAGGGCGGCCCGCGAATTCGCACGAGGGCACAAGGCTG
  GCATTTCTGAACCTTGCCCCTCTGCGAACACAAGGGGGCGATGGTGGCACTCCAAGCAAA
  GGGGCGTGTGGGTGCTGCAGGAGGAGCACAGAGCACTGGCGCCCCTCCCCTCCCGCCCTG
  CAGATGCCGGAGGCCCCGCCTCTGCTGTTGGCAGCTGTGTTGCTGGGCCTGGTGCTGCTG
  GTGGTGCTGCTGCTGCTTCTGAGGCACTGGGGCTGGGGCCTGTGCCTTATCGGCTGGAAC
  GAGTTCATCCTGCAGCCCATCCACAACCTGCTCATGGGTGACACCAAGGAGCAGCGCATC
  CTGAACCACGTGCTGCAGCATGCGGAGCCCGGGAACGCACAGAGCGTGCTGGAGGCCATT
  GACACCTACTGCGAGCAGAAGGAGTGGGCCATGAACGTGGGCGACAAGAAAGGCAAGATC
  GTGGACGCCGTGATTCAGGAGCACCAGCCCTCCGTGCTGCTGGAGCTGGGGGCCTACTGT
  GGCTACTCAGCTGTGCGCATGGCCCGCCTGCTGTCACCAGGGGCGAGGCTGATCACCATC
  GAGATCAACCCCGACTGTGCCGCCATCACCCAGCGGATGGTGGATTTCGCTGGCGTGAAG
  GACAGGTCACCCTTGTGGTTGGAGCGTCCCAGGACATCATCCCCCAGCTGAAGAAGAAGT
  ATGATGTGGACACACTGGACATGGTCTTCCTCGACCCTGGAAGGACCGTACCTGCCGNAC
  ACGCTTCTCTTGGNAGAATGTGNCTGCTGCNGAGGNGACAGTGCTACTGGCTGANCACGT
  GATCTGCCCCAGTGCGCCAGACTTCCTAGCACACGTGCGCGGGAGCAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007310 unedited
  GGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTCTATT
  TTATATTTTTAAAACATGATATTAGTATATAAGATAATATAGCTAGCCAGTGTTAGTAAA
  GAAGTCATGATTGAGTCTTAAAAAAGAACAATCCAGTGTTGCAGTTCAAAGAGGTTAGCA
  TGTCAGGGCGCAGGCCTCGGCCGAGGTGTGCTTTGCATTTAGGACACAGCCCGGAGCCGC
  AAAAGGTCAGCAGGAGCACGTCTGGCACCTTCAGTACCAGGCTGGGTGAAAGAGCCCGAG
  AGGGGGGCCGGGGGGGGCAGTCAGGGCCCTGCTTCGCTGCCTGGGCCCTTGTAAATGGCC
  TTCTCCAGGCCGTCCACCACCTCCCTGTATTCCAGGAACGATTGGTAGTGTGTGCACTCA
  AAGCAGCTGCTCCCGCGCACGTGTGCTAGGAAGTCTGGCGCACCTGGGCAGATCACGTTG
  TCAGCCAGTAGCACTGTCCCCTTCCGCAGCAGGCCACATTCCTCCAAGAGAAGCGTGTCC
  GGCAGGTACCGGTCCTTCCAGTGGTCGAGGAAGACCATGTCCAGTGTGTCCACATCATAC
  TTCTTCTTCAGCTGGGGGATGATGTCCTGNGACGCTCCAACCACAAGGTGACCTTGTCCT
  TCACGCCAGCGAAATCCACCATCCGCTGGGTGATGGCGGCACAGTCGGGGGTTGATCTCG
  ATGGTGATCAACCTCGCCCCCTGGGACAGCAAGCGGGCCATGCGCACAGCTGAGTACCCA
  CATAGGCCCCCACTCCAGCACCCGGAAGGCTGTGCCTCTGAATACAGAGTCCACATCTTG
  CTTTTTGTCGCCACGTTCATGGCCATTCTTCTGCTCCAGTAGGTGTAAGGCCTTCACACC
  CTTGGGCGTCCGGCCTCCCATGCTGAAACGTGTTCAGGACN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007310
• Insert Size: 1280 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007310.1, NP_009294.1
• RefSeq Size: 1067 bp
• RefSeq ORF: 666 bp
• Locus ID: 1312
• Cytogenetics: 22q11.21
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Tyrosine metabolism
• Gene Summary: 'Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]'
  Transcript Variant: This variant (4, also known as S-COMT) contains a shorter 5' UTR and a translation start site which lies 50 codons downstream compared to that of variant 1. The resulting isoform (S-COMT) is shorter at the N-terminus compared to isoform MB-COMT. S-COMT is a soluble protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.