GH2 (NM_022558) Human Untagged Clone

CAT#: SC109274

GH2 (untagged)-Human growth hormone 2 (GH2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GH2
• Synonyms: GH-V; GHB2; GHL; GHV; hGH-V
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_022558, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTGCAGGCTCCCGGACGTCCCTGCTCCTGGCTTTTGGCCTGCTCTGCCTGTCCTGGCTTCAAGAGG
  GCAGTGCCTTCCCAACCATTCCCTTATCCAGGCTTTTTGACAACGCTATGCTCCGCGCCCGTCGCCTGTA
  CCAGCTGGCATATGACACCTATCAGGAGTTTGAAGAAGCCTATATCCTGAAGGAGCAGAAGTATTCATTC
  CTGCAGAACCCCCAGACCTCCCTCTGCTTCTCAGAGTCTATTCCAACACCTTCCAACAGGGTGAAAACGC
  AGCAGAAATCTAACCTAGAGCTGCTCCGCATCTCCCTGCTGCTCATCCAGTCATGGCTGGAGCCCGTGCA
  GCTCCTCAGGAGCGTCTTCGCCAACAGCCTGGTGTATGGCGCCTCGGACAGCAACGTCTATCGCCACCTG
  AAGGACCTAGAGGAAGGCATCCAAACGCTGATAGGCTGGAAGATGGCAGCCCCCGGACTGGGCAGATCTT
  CAATCAGTCCTACAGCAAGTTTGACACAAAATCGCACAACGATGACGCACTGCTCAAGAACTACGGGCTG
  CTCTACTGCTTCAGGAAGGACATGGACAAGGTCGAGACATTCCTGCGCATCGTGCAGTGCCGCTCTGTGG
  AGGGCAGCTGTGGCTTCTAGCTGCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGTCGT
  GGAAGGTGCTACTCCAGTGCCCACCAGCCTTGTCCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022558 unedited
  TCAGAATTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGATAACAGCGAGA
  GAGAAGGGCCAGGTATAAAAAGGGCCCACAAGATACCAGCTCAAGGATCCCAAGGCCCAA
  CTCCCCGAACCACTCAAGGTCCTGTGGACAGCTCACCTATCGGCAATGGCTGCAGGCTCC
  CGGACGTCCCTGCTCCTGGCTTTTGGCCTGCTCTGCCTGTCCTGGCTTCAAGAGGGCAGT
  GCCTTCCCAACCATTCCCTTATCCAGGCTTTTTGACAACGCTATGCTCCGCGCCCGTCGC
  CTGTACCAGCTGGCATATGACACCTATCATGAGTTTGAAGAAGCCTATATCCTGAAGGAG
  CAGAAGTATTCATTCCTGCAGAACCCCCAGACCTCCCTCTGCTTCTCAGAGTCTATTCCA
  ACACCTTCCAACAGGGTGAAAACGCAGCATAAATCTAACCTATAGCTGCTCCGCATCTCC
  CTGCTGCTCATCCAGTCATGGCTGGAGCCCGTGCAGCTCCTCAAGAGCGTCTTCGCCAAC
  AGCCTGGTGTATGGCGCCTCGGACAGCAACGTCTATCGCCACCTGAAGGACCTATAGGAA
  GGCATCCAAACGCTGATAGGCTGGAAGATGGCAGCCCCCGGACTGGGCAGATCTTCAATC
  AGTCCTACAGCAAGTTTGACACATAATCGCACAACGATGACGCACTGCTCAAGAACTACN
  GGCTGCTCTACTGCTTCANGAAGACATGGACNANGTCGAGACATTCTGCGCATCGTGCAN
  TGCGCTCTGTAGAAGGCANCTGTGGCTTCTTCTGNCCCGGNTGCATNCCTGTGACCCNTN
  CCCATGCTCTNCTGGGCGGGGATGTGCTACNTCAGTGCCACCAGCCCTGTCCTATAAAAT
  TAAGTGGCTCAAAAAAAAAAAAAAACTCACCTAAATGNGGGCCGGNNATANCTGTCTGAA
  CAATACCCGGTGGGATCCTGGACCCTCCAGGGCTCGNGCCGCCGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022558
• Insert Size: 1000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022558.2, NP_072052.1
• RefSeq Size: 917 bp
• RefSeq ORF: 738 bp
• Locus ID: 2689
• Cytogenetics: 17q23.3
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, Neuroactive ligand-receptor interaction
• Gene Summary: 'The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. As in the case of its pituitary counterpart, growth hormone 1, the predominant isoform of this particular family member shows similar somatogenic activity, with reduced lactogenic activity. Mutations in this gene lead to placental growth hormone/lactogen deficiency. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) utilizes an alternative splice donor site in exon 4 causing a 4 nt deletion and a frameshift which generates an isoform (3) with a unique carboxy-terminus.