PLAT (NM_000930) Human Untagged Clone

CAT#: SC109576

PLAT (untagged)-Human plasminogen activator, tissue (PLAT), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLAT
• Synonyms: T-PA; TPA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000930 edited
  GAATTCGGCACGAGGCAGGGCTGGAGAGAAAACCTCTGCGAGGAAAGGGAAGGAGCAAGC
  CGTGAATTTAAGGGACGCTGTGAAGCAATCATGGATGCAATGAAGAGAGGGCTCTGCTGT
  GTGCTGCTGCTGTGTGGAGCAGTCTTCGTTTCGCCCAGCCAGGAAATCCATGCCCGATTC
  AGAAGAGGAGCCAGATCTTACCAAGTGATCTGCAGAGATGAAAAAACGCAGATGATATAC
  CAGCAACATCAGTCATGGCTGCGCCCTGTGCTCAGAAGCAACCGGGTGGAATATTGCTGG
  TGCAACAGTGGCAGGGCACAGTGCCACTCAGTGCCTGTCAAAAGTTGCAGCGAGCCAAGG
  TGTTTCAACGGGGGCACCTGCCAGCAGGCCCTGTACTTCTCAGATTTCGTGTGCCAGTGC
  CCCGAAGGATTTGCTGGGAAGTGCTGTGAAATAGATACCAGGGCCACGTGCTACGAGGAC
  CAGGGCATCAGCTACAGGGGCACGTGGAGCACAGCGGAGAGTGGCGCCGAGTGCACCAAC
  TGGAACAGCAGCGCGTTGGCCCAGAAGCCCTACAGCGGGCGGAGGCCAGACGCCATCAGG
  CTGGGCCTGGGGAACCACAACTACTGCAGAAACCCAGATCGAGACTCAAAGCCCTGGTGC
  TACGTCTTTAAGGCGGGGAAGTACAGCTCAGAGTTCTGCAGCACCCCTGCCTGCTCTGAG
  GGAAACAGTGACTGCTACTTTGGGAATGGGTCAGCCTACCGTGGCACGCACAGCCTCACC
  GAGTCGGGTGCCTCCTGCCTCCCGTGGAATTCCATGATCCTGATAGGCAAGGTTTACACA
  GCACAGAACCCCAGTGCCCAGGCACTGGGCCTGGGCAAACATAATTACTGCCGGAATCCT
  GATGGGGATGCCAAGCCCTGGTGCCACGTGCTGAAGAACCGCAGGCTGACGTGGGAGTAC
  TGTGATGTGCCCTCCTGCTCCACCTGCGGCCTGAGACAGTACAGCCAGCCTCAGTTTCGC
  ATCAAAGGAGGGCTCTTCGCCGACATCGCCTCCCACCCCTGGCAGGCTGCCATCTTTGCC
  AAGCACAGGAGGTCGCCCGGAGAGCGGTTCCTGTGCGGGGGCATACTCATCAGCTCCTGC
  TGGATTCTCTCTGCCGCCCACTGCTTCCAGGAGAGGTTTCCGCCCCACCACCTGACGGTG
  ATCTTGGGCAGAACATACCGGGTGGTCCCTGGCGAGGAGGAGCAGAAATTTGAAGTCGAA
  AAATACATTGTCCATAAGGAATTCGATGATGACACTTACGACAATGACATTGCGCTGCTG
  CAGCTGAAATCGGATTCGTCCCGCTGTGCCCAGGAGAGCAGCGTGGTCCGCACTGTGTGC
  CTTCCCCCGGCGGACCTGCAGCTGCCGGACTGGACGGAGTGTGAGCTCTCCGGCTACGGC
  AAGCATGAGGCCTTGTCCCCTTTCTATTCGGAGCGGCTGAAGGAGGCTCATGTCAGACTG
  TACCCATCCAGCCGCTGCACATCACAACATTTACTTAACAGAACAGTCACCGACAACATG
  CTGTGTGCTGGAGACACTCGGAGCGGCGGGCCCCAGGCAAACTTGCACGACGCCTGCCAG
  GGCGATTCGGGAGGCCCCCTGGTGTGTCTGAACGATGGCCGCATGACTTTGGTGGGCATC
  ATCAGCTGGGGCCTGGGCTGTGGACAGAAGGATGTCCCGGGTGTGTACACCAAGGTTACC
  AACTACCTAGACTGGATTCGTGACAACATGCGACCGTGACCAGGAACACCCGACTCCTCA
  AAAGCAAATGAGATCCCGCCTCTTCTTCTTCAGAAGACACTGCAAAGGCGCAGTGCTTCT
  CTACAGACTTCTCCAGACCCACCACACCGCAGAAGCGGGACGAGACCCTACAGGAGAGGG
  AAGAGTGCATTTTCCCAGATACTTCCCATTTTGGAAGTTTTCAGGACTTGGTCTGATTTC
  AGGATACTCTGTCAGATGGGAAGACATGAATGCACACTAGCCTCTCCAGGAATGCCTCCT
  CCCTGGGCAGAAAGTGGCCATGCCACCCTGTTTTCAGCTAAAGCCCAACCTCCTGACCTG
  TCACCGTGAGCAGCTTTGGAAACAGGACCACAAAAATGAAAGCATGTCTCAATAGTAAAA
  GATAACAAGATCTTTCAGGAAAGACGGATTGCATTAGAAATAGACAGTATATTTATAGTC
  ACAAGAGCCCAGCAGGGCCTCAAAGTTGGGGCAGGCTGGCTGGCCCGTCATGTTCCTCAA
  AAGCACCCTTGACGTCAAGTCTCCTTCCCCTTTCCCCACTCCCTGGCTCTCAGAAGGTAT
  TCCTTTTGTGTACAGTGTGTAAAGTGTAAATCCTTTTTCTTTATAAACTTTAGAGTAGCA
  TGAGAGAATTGTATCATTTGAACAACTAGGCTTCAGCATATTTATAGCAATCCATGTTAG
  TTTTTACTTTCTGTTGCCACAACCCTGTTTTATACTGTACTTAATAAATTCAGATATATT
  TTTCACAGTTTTTCCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000930 unedited
  NGGGGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGGGC
  TGGAGAGAAAACCTCTGCGAGGAAAGGGAAGGAGCAAGCCGTGAATTTAAGGGACGCTGT
  GAAGCAATCATGGATGCAATGAAGAGAGGGCTCTGCTGTGTGCTGCTGCTGTGTGGAGCA
  GTCTTCGTTTCGCCCAGCCAGGAAATCCATGCCCGATTCAGAAGAGGAGCCAGATCTTAC
  CAAGTGATCTGCAGAGATGAAAAAACGCAGATGATATACCAGCAACATCAGTCATGGCTG
  CGCCCTGTGCTCAGAAGCAACCGGGTGGAATATTGCTGGTGCAACAGTGGCAGGGCACAG
  TGCCACTCAGTGCCTGTCAAAAGTTGCAGCGAGCCAAGGTGTTTCAACGGGGGCACCTGC
  CAGCAGGCCCTGTACTTCTCAGATTTCGTGTGCCAGTGCCCCGAAGGATTTGCTGGGAAG
  TGCTGTGAAATAGATACCAGGGCCACGTGCTACGAGGACCAGGGCATCAGCTACAGGGGC
  ACGTGGAGCACAGCGGAGAGTGGCGCCGAGTGCACCAACTGGAACAGCAGCGCGTTGGCC
  CAGAAGCCCTACAGCGGGCGGAGGCCAGACGCCATCAGGCTGGGCCTGGGGAACCACAAC
  TACTGCAGAAACCCAGATCGAGACTCANAGCCCTGGTGCTACGTCTTTAAGGCGGNGAAG
  TACAGCTCAGAGTTCTGCAGCACCCCTGCCTGCTCTGNAGGAAACAGTGACTGCTACTTT
  NGGAATGGGTCAGCCTACCGTGGCACGCACAGCCTCACCGAGTCGGGTGCCTCCTGCCTC
  CCGTGGNAATCCATGATCCTGATANNGCAGGTTACACAGCACAGAACCCCATGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000930 unedited
  CGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTGGAAAAACTGTGAAAAATATATCTG
  AATTTATTAAGTACAGTATAAAACAGGGTTGTGGCAACAGAAAGTAAAAACTAACATGGA
  TTGCTATAAATATGCTGAAGCCTAGTTGTTCAAATGATACAATTCTCTCATGCTACTCTA
  AAGTTTATAAAGAAAAAGGATTTACACTTTACACACTGTACACAAAAGGAATACCTTCTG
  AGAGCCAGGGAGTGGGGAAAGGGGAAAGAGACTTGACGTCAAGGGTGCTTTTGAGGAACA
  TGACGGGCCAGCCAGCCTGCCCCAACTTTGAAGCCCTGCTGGGCTCTTGTGACTATAAAT
  ATACTGTCTATTTCTAATGCAATCCGTCTTTCCTGAAAGATCTTGTTATCTTTTACTATT
  GAGACATGCTTTCATTTTTGTGGTCCTGTTTCCAAAGCTGCTCACGGTGACAGGTCAAGA
  AGTTGGGCTTTAGCTGAAAACAAGGTGGCATGGCCACTTTCTGCCCAGGGAGGAGGCATT
  CCTGGAGAGGCTAGTGTGCATTCATGTCTTCCCATCTGACAGAGTATCCTGAAATCAGAC
  CAAGTCCTGAAAACTTCCAAAATGGGAAGTATCTGGGAAAATGCACTCTTCCCTCTNCTG
  TANGGTCTCGTCCAGCTTCTGCAGTGTGGTGGGTCTGGAGAAATCTGTAAAGTAGCACTG
  CGCCTTTGCAGTGTCTTCTGAAGAGAATGAGCAGGATCTCATTTGCCTTTGAGGAGTCCG
  GTGTTCTCTGTTCACCGTCGCATGTTGTCCGATTCCAGTCTTAGTAGGTGGTAACCTTGG
  NGTACACACCCGGNACATNCTTTTGTCCCAGCCCATGCCCAGCTGATGATGCCAACAATT
  CTGCGGNCATCGTTATACCCACAAGGGGCCTCCCGATCGCCCTGCAAGCGTNCTGCATTT
  TGCCTGGGCCCGCCCTTCCAATGGTTCACACAAACATGTTGTCGTGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000930
• Insert Size: 2660 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000930.2, NP_000921.1
• RefSeq Size: 2653 bp
• RefSeq ORF: 1689 bp
• Locus ID: 5327
• Cytogenetics: 8p11.21
• Domains: KR, FN1, Tryp_SPc, EGF
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: 'This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).