PLAT (NM_000931) Human Untagged Clone

CAT#: SC109577

PLAT (untagged)-Human plasminogen activator, tissue (PLAT), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLAT
• Synonyms: T-PA; TPA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000931 edited
  GAATTCGGCACGAGGCATGGATGCAATGAAGAGAGGGCTCTGCTGTGTGCTGCTGCTGTG
  TGGAGCAGTCTTCGTTTCGCCCAGCCAGGAAATCCATGCCCGATTCAGAAGAGGAGCCAG
  ATCTTACCAAGTGATCTGCAGAGATGAAAAAACGCAGATGATATACCAGCAACATCAGTC
  ATGGCTGCGCCCTGTGCTCAGAAGCAACCGGGTGGAATATTGCTGGTGCAACAGTGGCAG
  GGCACAGTGCCACTCAGTGCCTGTCAAAAGTTGCAGCGAGCCAAGGTGTTTCAACGGGGG
  CACCTGCCAGCAGGCCCTGTACTTCTCAGATTTCGTGTGCCAGTGCCCCGAAGGATTTGC
  TGGGAAGTGCTGTGAAATAGATACCAGGGCCACGTGCTACGAGGACCAGGGCATCAGCTA
  CAGGGGCACGTGGAGCACAGCGGAGAGTGGCGCCGAGTGCACCAACTGGAACAGCAGCGC
  GTTGGCCCAGAAGCCCTACAGCGGGCGGAGGCCAGACGCCATCAGGCTGGGCCTGGGGAA
  CCACAACTACTGCAGAAACCCAGATCGAGACTCAAAGCCCTGGTGCTACGTCTTTAAGGC
  GGGGAAGTACAGCTCAGAGTTCTGCAGCACCCCTGCCTGCTCTGAGGGAAACAGTGACTG
  CTACTTTGGGAATGGGTCAGCCTACCGTGGCACGCACAGCCTCACCGAGTCGGGTGCCTC
  CTGCCTCCCGTGGAATTCCATGATCCTGATAGGCAAGGTTTACACAGCACAGAACCCCAG
  TGCCCAGGCACTGGGCCTGGGCAAACATAATTACTGCCGGAATCCTGATGGGGATGCCAA
  GCCCTGGTGCCACGTGCTGAAGAACCGCAGGCTGACGTGGGAGTACTGTGATGTGCCCTC
  CTGCTCCACCTGCGGCCTGAGACAGTACAGCCAGCCTCAGTTTCGCATCAAAGGAGGGCT
  CTTCGCCGACATCGCCTCCCACCCCTGGCAGGCTGCCATCTTTGCCAAGCACAGGAGGTC
  GCCCGGAGAGCGGTTCCTGTGCGGGGGCATACTCATCAGCTCCTGCTGGATTCTCTCTGC
  CGCCCACTGCTTCCAGGAGAGGTTTCCGCCCCACCACCTGACGGTGATCTTGGGCAGAAC
  ATACCGGGTGGTCCCTGGCGAGGAGGAGCAGAAATTTGAAGTCGAAAAATACATTGTCCA
  TAAGGAATTCGATGATGACACTTACGACAATGACATTGCGCTGCTGCAGCTGAAATCGGA
  TTCGTCCCGCTGTGCCCAGGAGAGCAGCGTGGTCCGCACTGTGTGCCTTCCCCCGGCGGA
  CCTGCAGCTGCCGGACTGGACGGAGTGTGAGCTCTCCGGCTACGGCAAGCATGAGGCCTT
  GTCTCCTTTCTATTCGGAGCGGCTGAAGGAGGCTCATGTCAGACTGTACCCATCCAGCCG
  CTGCACATCACAACATTTACTTAACAGAACAGTCACCGACAACATGCTGTGTGCTGGAGA
  CACTCGGAGCGGCGGGCCCCAGGCAAACTTGCACGACGCCTGCCAGGGCGATTCGGGAGG
  CCCCCTGGTGTGTCTGAACGATGGCCGCATGACTTTGGTGGGCATCATCAGCTGGGGCCT
  GGGCTGTGGACAGAAGGATGTCCCGGGTGTGTACACCAAGGTTACCAACTACCTAGACTG
  GATTCGTGACAACATGCGACCGTGACCAGGAACACCCGACTCCTCAAAAGCAAATGAGAT
  CCCGCCTCTTCTTCTTCAGAAGACACTGCAAAGGCGCAGTGCTTCTCTACAGACTTCTCC
  AGACCCACCACACCGCAGAAGCGGGACGAGACCCTACAGGAGAGGGAAGAGTGCATTTTC
  CCAGATACTTCCCATTTTGGAAGTTTTCAGGACTTGGTCTGATTTCAGGATACTCTGTCA
  GATGGGAAGACATGAATGCACACTGGCCTCTCCAGGAATGCCTCCTCCCTGGGCAGAAAG
  TGGCCATGCCACCCTGTTTTCAGCTAAAGCCCAACCTCCTGACCTGTCACCGTGAGCAGC
  TTTGGAAACAGGACCACAAAAATGAAAGCATGTCTCAATAGTAAAAGATAACAAGATCTT
  TCAGGAAAGACGGATTGCATTAGAAATAGACAGTATATTTATAGTCACAAGAGCCCAGCA
  GGGCCTCAAAGTTGGGGCAGGCTGGCTGGCCCGTCATGTTCCTCAAAAGCACCCTTGACG
  TCAAGTCTCCTTCCCCTTTCCCCACTCCCTGGCTCTCAGAAGGTATTCCTTTTGTGTACA
  GTGTGTAAAGTGTAAATCCTTTTTCTTTATAAACTTTAGAGTAGCATGAGAGAATTGTAT
  CATTTGAACAACTAGGCTTCAGCATATTTATAGCAATCCATGTTAGTTTTTACTTTCTGT
  TGCCACAACCCTGTTTTATACTGTACTTAATAAATTCAGATATAAAAAAAAAAAAAAAAA
  AAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000931 unedited
  TGTTCGAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCATGGATGC
  AATGAAGAGAGGGCTCTGCTGTGTGCTGCTGCTGTGTGGAGCAGTCTTCGTTTCGCCCAG
  CCAGGAAATCCATGCCCGATTCAGAAGAGGAGCCAGATCTTACCAAGTGATCTGCAGAGA
  TGAAAAAACGCAGATGATATACCAGCAACATCAGTCATGGCTGCGCCCTGTGCTCAGAAG
  CAACCGGGTGGAATATTGCTGGTGCAACAGTGGCAGGGCACAGTGCCACTCAGTGCCTGT
  CAAAAGTTGCAGCGAGCCAAGGTGTTTCAACGGGGGCACCTGCCAGCAGGCCCTGTACTT
  CTCAGATTTCGTGTGCCAGTGCCCCGAAGGATTTGCTGGGAAGTGCTGTGAAATAGATAC
  CAGGGCCACGTGCTACGAGGACCAGGGCATCAGCTACAGGGGCACGTGGAGCACAGCGGA
  GAGTGGCGCCGAGTGCACCAACTGGAACAGCAGCGCGTTGGCCCAGAAGCCCTACAGCGG
  GCGGAGGCCAGACGCCATCAGGCTGGGCCTGGGGAACCACAACTACTGCAGAAACCCAGA
  TCGAGACTCANAGCCCTGGTGCTACGTCTTTAAGGCGGNGAAGTACAGCTCAGAGTTCTG
  CAGCACCCCTGCCTGCTCTGNAGGAAACAGTGACTGCTACTTTGNGAATGGGTCAGCCTA
  CCGTGGCACGCACAGCCTCACCGAGTCGGGTGCCTCCTGCCTCCCGTGGAATNCATGATC
  CTGATAGGGCAGGGTTACACAGCACAGAACCCCAGTGCCCCAGCACTGGGCCTGGGGCAN
  ACATATTACTGCCGGNATNCTGATGGGGATGCCCAGCCCTGGTGCCACGTGCTGAAAAAC
  CGCAGCTGACGTGGNAGTACTGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000931 unedited
  GTACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTATATCT
  GAATTTATTAAGTACAGTATAAAACAGGGTTGTGGCAACAGAAAGTAAAAACTAACATGG
  ATTGCTATAAATATGCTGAAGCCTAGTTGTTCAAATGATACAATTCTCTCATGCTACTCT
  AAAGTTTATAAAGAAAAAGGATTTACACTTTACACACTGTACACAAAAGGAATACCTTCT
  GAGAGCCAGGGAGTGGGGAAAGGGGAAGGAGACTTGACGTCAAGGGTGCTTTTGAGGAAC
  ATGACGGGCCAGCCAGCCTGCCCCAACTTTGAGGCCCTGCTGGGCTCTTGTGACTATAAA
  TATACTGTCTATTTCTAATGCAATCCGTCTTTCCTGAAAGATCTTGTTATCTTTTACTAT
  TGAGACATGCTTTCATTTTTGTGGTCCTGTTTCCAAAGCTGCTCACGGTGACAGGTCAGG
  AGGTTGGGCTTTAGCTGAAAACAGGGTGGCATGGCCACTTTCTGCCCAGGGAGGAGGCAT
  TCCTGGAGAGGCCAGTGTGCATTCATGTCTTCCCATCTGACAGAGTATCCTGAAATCAGA
  CCAAGTCCTGAAAACTTCCCAAATGGGAAGTATCTGGGAAAATGCCCTCTTCCCTTTCCT
  GTAAGGGCTCGCCCCGCTTTTGCGGTGTGGTGGGCTTGAAGAATCCTGTTACAAAACCTC
  GCCCCTTTGCCATGCTTTCTGAACAACAAAAGGCGGATCTCCATTGCTTTTGAAGAGCTC
  GGCGTTCCCGGCAACGGCCCCTCGTCCCACCATCCCTCTAAGGACTTGGTCACCTTGGGC
  CCCCCCCGGACACCTTCTGCCACACTCAGCTCACCTGTCGTAGCCCCCCAACCTGCGCTC
  TCGTCCAACCACCCGGGCTCCATCCACAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000931
• Insert Size: 2600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000931.1, NP_000922.1
• RefSeq Size: 2461 bp
• RefSeq ORF: 2160 bp
• Locus ID: 5327
• Cytogenetics: 8p11.21
• Domains: KR, FN1, EGF
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Complement and coagulation cascades
• Gene Summary: 'This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (2) lacks exons 9, 10, and 11, and uses an alternate splice acceptor site in exon 12 resulting in a frameshift, as compared to variant 1. It encodes isoform 2 which has a distinct C-terminus as compared to the other isoforms and likely does not form a two chain disulfide linked protein like isoform 1.