PLAT (NM_000931) Human Untagged Clone

CAT#: SC109577

PLAT (untagged)-Human plasminogen activator, tissue (PLAT), transcript variant 2


Reconstitution Protocol

USD 660.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "PLAT"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PLAT
Synonyms T-PA; TPA
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000931 edited
GAATTCGGCACGAGGCATGGATGCAATGAAGAGAGGGCTCTGCTGTGTGCTGCTGCTGTG
TGGAGCAGTCTTCGTTTCGCCCAGCCAGGAAATCCATGCCCGATTCAGAAGAGGAGCCAG
ATCTTACCAAGTGATCTGCAGAGATGAAAAAACGCAGATGATATACCAGCAACATCAGTC
ATGGCTGCGCCCTGTGCTCAGAAGCAACCGGGTGGAATATTGCTGGTGCAACAGTGGCAG
GGCACAGTGCCACTCAGTGCCTGTCAAAAGTTGCAGCGAGCCAAGGTGTTTCAACGGGGG
CACCTGCCAGCAGGCCCTGTACTTCTCAGATTTCGTGTGCCAGTGCCCCGAAGGATTTGC
TGGGAAGTGCTGTGAAATAGATACCAGGGCCACGTGCTACGAGGACCAGGGCATCAGCTA
CAGGGGCACGTGGAGCACAGCGGAGAGTGGCGCCGAGTGCACCAACTGGAACAGCAGCGC
GTTGGCCCAGAAGCCCTACAGCGGGCGGAGGCCAGACGCCATCAGGCTGGGCCTGGGGAA
CCACAACTACTGCAGAAACCCAGATCGAGACTCAAAGCCCTGGTGCTACGTCTTTAAGGC
GGGGAAGTACAGCTCAGAGTTCTGCAGCACCCCTGCCTGCTCTGAGGGAAACAGTGACTG
CTACTTTGGGAATGGGTCAGCCTACCGTGGCACGCACAGCCTCACCGAGTCGGGTGCCTC
CTGCCTCCCGTGGAATTCCATGATCCTGATAGGCAAGGTTTACACAGCACAGAACCCCAG
TGCCCAGGCACTGGGCCTGGGCAAACATAATTACTGCCGGAATCCTGATGGGGATGCCAA
GCCCTGGTGCCACGTGCTGAAGAACCGCAGGCTGACGTGGGAGTACTGTGATGTGCCCTC
CTGCTCCACCTGCGGCCTGAGACAGTACAGCCAGCCTCAGTTTCGCATCAAAGGAGGGCT
CTTCGCCGACATCGCCTCCCACCCCTGGCAGGCTGCCATCTTTGCCAAGCACAGGAGGTC
GCCCGGAGAGCGGTTCCTGTGCGGGGGCATACTCATCAGCTCCTGCTGGATTCTCTCTGC
CGCCCACTGCTTCCAGGAGAGGTTTCCGCCCCACCACCTGACGGTGATCTTGGGCAGAAC
ATACCGGGTGGTCCCTGGCGAGGAGGAGCAGAAATTTGAAGTCGAAAAATACATTGTCCA
TAAGGAATTCGATGATGACACTTACGACAATGACATTGCGCTGCTGCAGCTGAAATCGGA
TTCGTCCCGCTGTGCCCAGGAGAGCAGCGTGGTCCGCACTGTGTGCCTTCCCCCGGCGGA
CCTGCAGCTGCCGGACTGGACGGAGTGTGAGCTCTCCGGCTACGGCAAGCATGAGGCCTT
GTCTCCTTTCTATTCGGAGCGGCTGAAGGAGGCTCATGTCAGACTGTACCCATCCAGCCG
CTGCACATCACAACATTTACTTAACAGAACAGTCACCGACAACATGCTGTGTGCTGGAGA
CACTCGGAGCGGCGGGCCCCAGGCAAACTTGCACGACGCCTGCCAGGGCGATTCGGGAGG
CCCCCTGGTGTGTCTGAACGATGGCCGCATGACTTTGGTGGGCATCATCAGCTGGGGCCT
GGGCTGTGGACAGAAGGATGTCCCGGGTGTGTACACCAAGGTTACCAACTACCTAGACTG
GATTCGTGACAACATGCGACCGTGACCAGGAACACCCGACTCCTCAAAAGCAAATGAGAT
CCCGCCTCTTCTTCTTCAGAAGACACTGCAAAGGCGCAGTGCTTCTCTACAGACTTCTCC
AGACCCACCACACCGCAGAAGCGGGACGAGACCCTACAGGAGAGGGAAGAGTGCATTTTC
CCAGATACTTCCCATTTTGGAAGTTTTCAGGACTTGGTCTGATTTCAGGATACTCTGTCA
GATGGGAAGACATGAATGCACACTGGCCTCTCCAGGAATGCCTCCTCCCTGGGCAGAAAG
TGGCCATGCCACCCTGTTTTCAGCTAAAGCCCAACCTCCTGACCTGTCACCGTGAGCAGC
TTTGGAAACAGGACCACAAAAATGAAAGCATGTCTCAATAGTAAAAGATAACAAGATCTT
TCAGGAAAGACGGATTGCATTAGAAATAGACAGTATATTTATAGTCACAAGAGCCCAGCA
GGGCCTCAAAGTTGGGGCAGGCTGGCTGGCCCGTCATGTTCCTCAAAAGCACCCTTGACG
TCAAGTCTCCTTCCCCTTTCCCCACTCCCTGGCTCTCAGAAGGTATTCCTTTTGTGTACA
GTGTGTAAAGTGTAAATCCTTTTTCTTTATAAACTTTAGAGTAGCATGAGAGAATTGTAT
CATTTGAACAACTAGGCTTCAGCATATTTATAGCAATCCATGTTAGTTTTTACTTTCTGT
TGCCACAACCCTGTTTTATACTGTACTTAATAAATTCAGATATAAAAAAAAAAAAAAAAA
AAAAAAAAACTCGAC
>OriGene 5' read for NM_000931 unedited
TGTTCGAATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCATGGATGC
AATGAAGAGAGGGCTCTGCTGTGTGCTGCTGCTGTGTGGAGCAGTCTTCGTTTCGCCCAG
CCAGGAAATCCATGCCCGATTCAGAAGAGGAGCCAGATCTTACCAAGTGATCTGCAGAGA
TGAAAAAACGCAGATGATATACCAGCAACATCAGTCATGGCTGCGCCCTGTGCTCAGAAG
CAACCGGGTGGAATATTGCTGGTGCAACAGTGGCAGGGCACAGTGCCACTCAGTGCCTGT
CAAAAGTTGCAGCGAGCCAAGGTGTTTCAACGGGGGCACCTGCCAGCAGGCCCTGTACTT
CTCAGATTTCGTGTGCCAGTGCCCCGAAGGATTTGCTGGGAAGTGCTGTGAAATAGATAC
CAGGGCCACGTGCTACGAGGACCAGGGCATCAGCTACAGGGGCACGTGGAGCACAGCGGA
GAGTGGCGCCGAGTGCACCAACTGGAACAGCAGCGCGTTGGCCCAGAAGCCCTACAGCGG
GCGGAGGCCAGACGCCATCAGGCTGGGCCTGGGGAACCACAACTACTGCAGAAACCCAGA
TCGAGACTCANAGCCCTGGTGCTACGTCTTTAAGGCGGNGAAGTACAGCTCAGAGTTCTG
CAGCACCCCTGCCTGCTCTGNAGGAAACAGTGACTGCTACTTTGNGAATGGGTCAGCCTA
CCGTGGCACGCACAGCCTCACCGAGTCGGGTGCCTCCTGCCTCCCGTGGAATNCATGATC
CTGATAGGGCAGGGTTACACAGCACAGAACCCCAGTGCCCCAGCACTGGGCCTGGGGCAN
ACATATTACTGCCGGNATNCTGATGGGGATGCCCAGCCCTGGTGCCACGTGCTGAAAAAC
CGCAGCTGACGTGGNAGTACTGG
>OriGene 3' read for NM_000931 unedited
GTACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTATATCT
GAATTTATTAAGTACAGTATAAAACAGGGTTGTGGCAACAGAAAGTAAAAACTAACATGG
ATTGCTATAAATATGCTGAAGCCTAGTTGTTCAAATGATACAATTCTCTCATGCTACTCT
AAAGTTTATAAAGAAAAAGGATTTACACTTTACACACTGTACACAAAAGGAATACCTTCT
GAGAGCCAGGGAGTGGGGAAAGGGGAAGGAGACTTGACGTCAAGGGTGCTTTTGAGGAAC
ATGACGGGCCAGCCAGCCTGCCCCAACTTTGAGGCCCTGCTGGGCTCTTGTGACTATAAA
TATACTGTCTATTTCTAATGCAATCCGTCTTTCCTGAAAGATCTTGTTATCTTTTACTAT
TGAGACATGCTTTCATTTTTGTGGTCCTGTTTCCAAAGCTGCTCACGGTGACAGGTCAGG
AGGTTGGGCTTTAGCTGAAAACAGGGTGGCATGGCCACTTTCTGCCCAGGGAGGAGGCAT
TCCTGGAGAGGCCAGTGTGCATTCATGTCTTCCCATCTGACAGAGTATCCTGAAATCAGA
CCAAGTCCTGAAAACTTCCCAAATGGGAAGTATCTGGGAAAATGCCCTCTTCCCTTTCCT
GTAAGGGCTCGCCCCGCTTTTGCGGTGTGGTGGGCTTGAAGAATCCTGTTACAAAACCTC
GCCCCTTTGCCATGCTTTCTGAACAACAAAAGGCGGATCTCCATTGCTTTTGAAGAGCTC
GGCGTTCCCGGCAACGGCCCCTCGTCCCACCATCCCTCTAAGGACTTGGTCACCTTGGGC
CCCCCCCGGACACCTTCTGCCACACTCAGCTCACCTGTCGTAGCCCCCCAACCTGCGCTC
TCGTCCAACCACCCGGGCTCCATCCACAC
Restriction Sites NotI-NotI     
ACCN NM_000931
Insert Size 2600 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000931.1, NP_000922.1
RefSeq Size 2461 bp
RefSeq ORF 2160 bp
Locus ID 5327
Cytogenetics 8p11.21
Domains KR, FN1, EGF
Protein Families Druggable Genome, Protease, Secreted Protein
Protein Pathways Complement and coagulation cascades
Gene Summary 'This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]'
Transcript Variant: This variant (2) lacks exons 9, 10, and 11, and uses an alternate splice acceptor site in exon 12 resulting in a frameshift, as compared to variant 1. It encodes isoform 2 which has a distinct C-terminus as compared to the other isoforms and likely does not form a two chain disulfide linked protein like isoform 1.

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.