FXYD1 (NM_021902) Human Untagged Clone

CAT#: SC109579

FXYD1 (untagged)-Human FXYD domain containing ion transport regulator 1 (FXYD1), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FXYD1
• Synonyms: PLM
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109579 sequence for NM_021902 edited (data generated by NextGen Sequencing)
  ATGGCGTCTCTTGGCCACATCTTGGTTTTCTGTGTGGGTCTCCTCACCATGGCCAAGGCA
  GAAAGTCCAAAGGAACACGACCCGTTCACTTACGACTACCAGTCCCTGCAGATCGGAGGC
  CTCGTCATCGCCGGGATCCTCTTCATCCTGGGCATCCTCATCGTGCTGAGCAGAAGATGC
  CGGTGCAAGTTCAACCAGCAGCAGAGGACTGGGGAACCCGATGAAGAGGAGGGAACTTTC
  CGCAGCTCCATCCGCCGTCTGTCCACCCGCAGGCGGTAG
  
  Clone variation with respect to NM_021902.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021902 unedited
  TTCGGCACGAGGCTCAGCCCCCGGGGCACAGCAGGACGTTTGGGGGCCTTCTTTCAGCAG
  GGGACAGCCCGATTGGGGACAATGGCGTCTCTTGGCCACATCTTGGTTTTCTGTGTGGGT
  CTCCTCACCATGGCCAAGGCAGAAAGTCCAAAGGAACACGACCCGTTCACTTACGACTAC
  CAGTCCCTGCATATCGGAGGCCTCGTCATCGCCGGGATCCTCTTCATCCTGGGCATCCTC
  ATCGTGCTGAGCAGAAGATGCCGGTGCAAGTTCAACCAGCAGCAGAGGACTGGGGAACCC
  GATGAAGAGGAGGGAACTTTCCGCAGCTCCATCCGCCGTCTGTCCACCCGCAGGCGGTAG
  AAACACCTGGAGCGATGGAATCCGGCCAGGACTCCCCTGGCACCTGACATCTCCCACGCT
  CACACTGCGGGCNCCACCGGCCCCTTCCGCGGCCCCTTTCCCAGGCCCTGCCCCCGCAGA
  CTCCCCCTGCCGCCAAGACTTCCAATAAAACGTGCGTTCCTCTCGACAAAAAAAAAAAAA
  AAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGGTNCCTGAACAGATCCCGGGTG
  GCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCC
  CACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTA
  TATATTATGGGNGGAGGGGGGGGGGGGTTTNNACCNAAGGGGGAATTTGGGAGAAAAAAC
  CCTGTAGGGCCCGGGNGGTCTTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021902 unedited
  CCCCCCCCCCTTAGGTTTGAACCGCGGCTCGCATCCTACGATCGAGTTTTTTTTTTTTTT
  TTTTGTCGAGAGGACACGCACGTTTTATTGGAAGTCTTGGCGGCAGGGGGAGTCTGCGGG
  GGCAGGGCTGGGGAAGGGGCGGCGGAGGGGGCGGTGGGCGCGCAGGTGGAGCGTGGGAGA
  TGTCAGGTGCCAGGGGAGTCCTGGCCGGATTCCATCGCTCCAGGTGTTTCTACCGCCTGC
  GGGTGGACAGACGGCGGATGGAGCTGCGGAAAGTTCCCTCCTCTTCATCGGGTTCCCCAG
  TCCTCTGCTGCTGGTTGAACTTGCACCGGCATCTTCTGCTCAGCACGATGACGATGCCCA
  CGATGAAGAGGATCCCGGCCTTGACTAGGTCTCCGATCTGTTGGGACTGTTTAACTTACG
  TGCACGCGCCCGGGCCACTCTTTCCCTCTCGTCCTGTCCCCTTCGTTCGCCACTTTCTCC
  TCTCCCCCTCCGCTCCCTCCTCCTCCTTTCTTCTCTTCTCTTTCTTCCTTTTCCCCTCTT
  CCCTTTCCTCTCCTCATCATTTCTTCCCCTTCTACTCCCTTTCTCGNCCCCTCCACTCCT
  TCTTCTCCCCTCCTTTTTCTCCTTCTCCTCACCTTCTCTATCCTCACCCCTCACTCATCC
  CCCCCTCACCCTCTCCTCTAACTCCCCTCTTCTCCTCCCCCTTCCTCTCCCTTTTCCCTT
  TCTCACCCTCTTCCTCTCCCTTTTCCATCCCCCTCCCCCTCCCTCCCCTTCTCTCTTCTC
  CTTTTCTCTTTCCCCCCCTCTCCTCTCCTATCCCCCCCTTCCATATTCTCCTCTTCTCCC
  CTTCCTTTTTTTTTCCCTCTACCCCTTCCCCCTCTTCTCTAATTCCCCTTCTTCTTTTCC
  TCTCCCCCCTTATTATCTCTTAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021902
• Insert Size: 580 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021902.2, NP_068702.1
• RefSeq Size: 558 bp
• RefSeq ORF: 279 bp
• Locus ID: 5348
• Cytogenetics: 19q13.12
• Domains: ATP1G1_PLM_MAT8
• Protein Families: Ion Channels: Other, Transmembrane
• Gene Summary: 'This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. The protein encoded by this gene is a plasma membrane substrate for several kinases, including protein kinase A, protein kinase C, NIMA kinase, and myotonic dystrophy kinase. It is thought to form an ion channel or regulate ion channel activity. Transcript variants with different 5' UTR sequences have been described in the literature. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (b) differs in the 5' UTR compared to variant a. Variants a, b, c and d encode the same protein.