Papillary renal cell carcinoma (PRCC) (NM_005973) Human Untagged Clone

CAT#: SC109612

PRCC (untagged)-Human papillary renal cell carcinoma (translocation-associated) (PRCC)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRCC
• Synonyms: RCCP1; TPRC
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109612 sequence for NM_005973 edited (data generated by NextGen Sequencing)
  ATGTCGCTGGTTGCTTACGCCAGCAGCGATGAGAGCGAGCCGGATGAGGCTGAGCCCGAG
  CCGGAGGAAGAGGAGGCGGTGGCTCCTACATCTGGGCCCGCTTTAGGGGGCTTGTTCGCT
  TCTCTCCCTGCGCCCAAGGGTCCGGCCTTGCTGCCTCCGCCCCCTCAGATGCTGGCGCCA
  GCCTTTCCCCCGCCGCTGTTGCTTCCCCCACCCACCGGAGACCCCAGGCTTCAGCCTCCT
  CCCCCCTTGCCCTTCGGCCTGGGAGGCTTCCCCCCACCTCCAGGCGTGAGCCCGGCTGAA
  GCGGCGGGAGTTGGGGAGGGACTGGGATTGGGGTTGCCCTCGCCCCGAGGCCCTGGCCTC
  AATCTGCCCCCTCCAATTGGCGGTGCCGGTCCCCCGCTGGGGCTTTCCAAGCCAAAGAAG
  AGGAAAGAGCCCGTGAAGATCGCGGCGCCGGAGTTGCATAAGGGAGATTCAGATTCTGAG
  GAAGATGAACCCACAAAGAAGAAAACTATCCTTCAGGGATCCAGTGAGGGGACTGGTTTG
  TCTGCCTTGCTTCCCCAACCTAAAAACCTGACTGTGAAAGAGACTAACAGGTTGCTCCTG
  CCCCATGCCTTCTCCCGCAAACCCTCGGATGGCTCCCCTGATACTAAGCCCTCCAGACTG
  GCTTCTAAGACCAAGACTTCCTCTCTTGCCCCTGTTGTGGGCACCACAACCACCACTCCG
  TCGCCCTCTGCTATCAAGGCTGCTGCCAAGAGTGCTGCCCTGCAGGTGACAAAGCAGATC
  ACGCAGGAAGAAGACGACAGTGATGAGGAAGTAGCCCCCGAAAACTTTTTCTCCCTCCCT
  GAAAAGGCTGAGCCACCTGGAGTTGAGCCATACCCTTACCCCATCCCCACTGTCCCTGAA
  GAGCTGCCTCCAGGCACGGAACCAGAGCCGGCTTTCCAGGACGATGCAGCCAATGCCCCC
  CTTGAATTCAAGATGGCAGCAGGTTCAAGTGGGGCCCCTTGGATGCCTAAGCCTGGGGAC
  GACTACAGCTACAATCAGTTTTCCACATATGGCGATGCCAATGCCGCTGGTGCTTATTAT
  CAGGATTATTACAGTGGTGGCTACTATCCTGCACAGGACCCGGCCCTGGTCCCCCCCCAG
  GAAATTGCCCCAGATGCCTCCTTCATCGATGACGAAGCATTTAAGCGGCTGCAGGGCAAG
  AGGAACCGAGGGAGAGAAGAAATCAACTTTGTGGAGATCAAAGGTGATGACCAGCTCAGT
  GGGGCCCAGCAATGGATGACTAAGTCATTGACAGAAGAGAAAACCATGAAGTCATTCAGC
  AAAAAGAAAGGTGAGCAGCCAACAGGCCAGCAGCGGCGGAAACACCAGATCACATATCTT
  ATTCATCAGGCCAAGGAGCGGGAGCTGGAACTGAAGAACACCTGGTCAGAGAACAAGCTC
  AGCCGCCGTCAGACCCAAGCCAAATATGGATTCTAG
  
  Clone variation with respect to NM_005973.4
  406 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005973 unedited
  CCCGCCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAG
  CTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATT
  CGGCACGAGGGGGACTAGGAGCTTAAGTGAAGAGGTACGCCTTGTTCGGTGGAAATCAGC
  CGTAGCCATGAGTTTCTGCCGGGGCTAGCCCTAGAGTACGGAGCAGGCGGACTTTTCGGT
  TCCCCGCCCCGCCAGGTGGCGGGGCCTACTAGGCCTCCGGGCATCCCCGGTCTCAAGTAG
  GCCTCATCTGCCGGCAAGGGCGCCCGAAACGCGGGAGGCGCCATGTCGCTGGTTGCTTAC
  GCCAGCAGCGATGAGAGCGAGCCGGATGAGGCTGAGCCCGAGCCGGAGGAAGAGGAGGCG
  GTGGCTCCTACATCTGGGCCCGCTTTAGGGGGCTTGTTCGCTTCTCTCCCTGCGCCCAAG
  GGTCCGGCCTTGCTGCCTCCGCCCCCTCAGATGCTGGCGCCAGCCTTTCCCCCGCCGCTG
  TTGCTTCCCCCACCCACCGGAGACCCCAGGCTTCAGCCTCCTCCCCCCTTGCCCTTCGGC
  CTGGGAGGCTTCCCCCCACCTCCAGGCGTGAGCCCGGCTGAAGCGGCGGGAGTTGGGGAG
  GGACTGGGATTGGGGTTGCCCTCGCCCCGAGGCCCTGGGCCTCATCTGCCCCCTCCATTG
  GCGGTGCCGGTCCCCCGCTGGGGCTTNTCCAGCCAAAGAAGAAGAAAGAGCCCGTGNAAG
  ATCGCGCGCCCGAGTTGCATAAGGGAGATTCAGATTTCTGAGAAGATGAACCCACAAGAA
  GAAACTATCCTTCAGGNATCAGTGAGGGGACTGGTTNNGTCTGCTTNNGCTCCCACCTAA
  AAACTGACTGTGAAGAGACTAACAGTTTGCTCTGCCCATGCCTTCTCCGCAACCCTCGGA
  TGCTCCCTGAACTAAGCCCTCGACTGGCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005973 unedited
  NAGTTTTAAAACCCTTTTTTTTGGCAAACTGAATGTTTTATTTCAACAGACATCCCTGGT
  TTAAAAAAAAAAAGTGGTGGTGGGGAGGGTTCAACATTTTATCACACCTGACAGAGAGCA
  AAACTCCCACCCCAGAACTCAGAGCCCCTTCGGAGGCATCACACAAGTCTTTGCTAGCTC
  CAGGACATTTAGGAGCTGAATTTTCTAACTTGGGAAGAAAAATCTATCAAATATGTTCAT
  TCTCGCAGAGGCGAGGGCTGGGTCCTTGGGGAAAGAGATCCTGGGCTTAGAGCAGCTGGG
  GTCCCAGAGGTGAAGCTGGGGGCCAGGCCAGCTGGGCTGGCAGGAGATCCTGGGAGCAAT
  CAGTTCCAGAGCCCTAGAATCCATATTTGGCTTGGGTCTGACGGCGGCTGAGCTTGTTCT
  CTGACCAGGTGTTCTTCAGTTCCAGCTCCCGCTCCTTGGCCTGATGAATAAGATATGTGA
  TCTGGTGTTTCCGCCGCTGCTGGCCTGTTGGCTGCTCACCTTTCTTTTTGCTGAATGACT
  TCATGGTTTTCTCTTCTGTCAATGACTTAGTCATCCATTGCTGGGCCCCACTGAGCTGGT
  CATCACCTTTGATCTCCACAAAGTTGATTTCTTCTCTCCCTCGGTTCCTCTTGCCCTGCA
  GCCGCTTAAATGGCTCGTCATCGATGAAGGAGGCATCTGGGGGCAATTTCCTGGGGGGGG
  GACCAGGGGCCGGGTCCCTGTGCANGATAGTAGCCCACACTGTTATAATCCTGATNATTA
  AGCACAGCGGCATTGGCATCGCCATATGTGGAATACTGATTGTAGCTGTAGTCCGTCCCC
  AGGCTAAGGATCCAAGGGGCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005973
• Insert Size: 2150 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005973.4, NP_005964.3
• RefSeq Size: 2142 bp
• RefSeq ORF: 1476 bp
• Locus ID: 5546
• Cytogenetics: 1q23.1
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a protein that may play a role in pre-mRNA splicing. Chromosomal translocations (X;1)(p11;q21) that result in fusion of this gene to TFE3 (GeneID 7030) have been associated with papillary renal cell carcinoma. A PRCC-TFE3 fusion protein is expressed in affected carcinomas and is likely associated with altered gene transactivation. This fusion protein has also been associated with disruption of the cell cycle.[provided by RefSeq, Aug 2010]'