JNK3 (MAPK10) (NM_138982) Human Untagged Clone

CAT#: SC109627

MAPK10 (untagged)-Human mitogen-activated protein kinase 10 (MAPK10), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAPK10
• Synonyms: JNK3; JNK3A; p54bSAPK; p493F12; PRKM10; SAPK1b
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_138982 edited
  ATGAGCCTCCATTTCTTATACTACTGCAGTGAACCAACATTGGATGTGAAAATTGCCTTT
  TGTCAGGGATTCGATAAACAAGTGGATGTGTCATATATTGCCAAACATTACAACATGAGC
  AAAAGCAAAGTTGACAACCAGTTCTACAGTGTGGAAGTGGGAGACTCAACCTTCACAGTT
  CTCAAGCGCTACCAGAATCTAAAGCCTATTGGCTCTGGGGCTCAGGGCATAGTTTGTGCC
  GCGTATGATGCTGTCCTTGACAGAAATGTGGCCATTAAGAAGCTCAGCAGACCCTTTCAG
  AACCAAACACATGCCAAGAGAGCGTACCGGGAGCTGGTCCTCATGAAGTGTGTGAACCAT
  AAAAACATTATTAGTTTATTAAATGTCTTCACACCCCAGAAAACGCTGGAGGAGTTCCAA
  GATGTTTACTTAGTAATGGAACTGATGGATGCCAACTTATGTCAAGTGATTCAGATGGAA
  TTAGACCATGAGCGAATGTCTTACCTGCTGTACCAAATGTTGTGTGGCATTAAGCACCTC
  CATTCTGCTGGAATTATTCACAGGGATTTAAAACCAAGTAACATTGTAGTCAAGTCTGAT
  TGCACATTGAAAATCCTGGACTTTGGACTGGCCAGGACAGCAGGCACAAGCTTCATGATG
  ACTCCATATGTGGTGACACGTTATTACAGAGCCCCTGAGGTCATCCTGGGGATGGGCTAC
  AAGGAGAACGTGGATATATGGTCTGTGGGATGCATTATGGGAGAAATGGTTCGCCACAAA
  ATCCTCTTTCCAGGAAGGGACTATATTGACCAGTGGAATAAGGTAATTGAACAACTAGGA
  ACACCATGTCCAGAATTCATGAAGAAATTGCAACCCACAGTAAGAAACTATGTGGAGAAT
  CGGCCCAAGTATGCGGGACTCACCTTCCCCAAACTCTTCCCAGATTCCCTCTTCCCAGCG
  GACTCCGAGCACAATAAACTCAAAGCCAGCCAAGCCAGGGACTTGTTGTCAAAGATGCTA
  GTGATTGACCCAGCAAAAAGAATATCAGTGGACGACGCCTTACAGCATCCCTACATCAAC
  GTCTGGTATGACCCAGCCGAAGTGGAGGCGCCTCCACCTCAGATATATGACAAGCAGTTG
  GATGAAAGAGAACACACAATTGAAGAATGGAAAGAACTTATCTACAAGGAAGTAATGAAT
  TCAGAAGAAAAGACTAAAAATGGTGTAGTAAAAGGACAGCCTTCTCCTTCAGGTGCAGCA
  GTGAACAGCAGTGAGAGTCTCCCTCCATCCTCGTCTGTCAATGACATCTCCTCCATGTCC
  ACCGACCAGACCCTGGCATCTGACACTGACAGCAGCCTGGAAGCCTCGGCAGGACCCCTG
  GGTTGTTGCAGGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_138982 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTTCTTGGAAATCAC
  CTGTCCATCGTTAATTTTAAACAATCTCCATATCTCCAAAGAATCTCTTCCATGTTAGTC
  TGGAATGTGGTTAATGAAAAACAAGTAGGGAGGATTTCTGGGGCAAACACTGCCGGATCA
  GGATCGTAGTTCTCAGGCACGGAATGGCTAGTGTGAGAAACACCAACAGCAGGCCCATCT
  CAGATCTTCACTATGGCAACTTATGCAAGAAACTGTTGAATTAGACCCGTTTCCTATAGA
  TGAGAAACCATACAAGCTGTGGTATTTATGAGCCTCCATTTCTTATACTACTGCAGTGAA
  CCAACATTGGATGTGAAAATTGCCTTTTGTCAGGGATTCGATAAACAAGTGGATGTGTCA
  TATATTGCCAAACATTACAACATGAGCAAAAGCAAAGTTGACAACCAGTTCTACAGTGTG
  GAAGTGGGAGACTCAACCTTCACAGTTCTCAAGCGCTACCAGAATCTAAAGCCTATTGGC
  TCTGGGGCTCAGGGCATAGTTTGTGCCGCGTATGATGCTGTCCTTGACAGAAATGTGGCC
  ATTAAGAAGCTCAGCAGACCCTTTCAGAACCAAACACATGCCAAGAGAGCGTACCGGGAG
  CTGGTCCTCATGAAGTGTGTGAACCATAAAAACATTATTAGTTTATTAAATGTCTTCACA
  CCCCAGAAAACGCTGGNAGGAGTTCCAAAGATGTTACTTAGTNATGGAACTGATGGATGC
  CAACTTTATGTCAGTGATTCAGATGGAATTAGACCATGAGCGAATGTCTTACCTGCTGTA
  CAAATGGTTGTGTGCATTAAGCACCTNCATCTGCTGGNATATCACAGGATTAAACCAGTA
  CATGNAGTCAGTCTGATGCCATTGAAANCTGACTTTGACTGGCAGAAGANGACAGCTCAT
  GTACTCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_138982 unedited
  AGGTGCCATTTATNCCTTCAAATTGGAGCTACATCTTCAAGGTCTGAGAGAGCTCACTCC
  CCCCTATATTCCCCCTTTACATGTTTTCTTATAAGACATACAGTTTAATCAATTAACAAA
  CTAAACAGCTTATATACTGGCAATATATTACAGATGGGTTTATGTCAGAGTAATAGATCA
  CATGAAATGGACCATGTGGTACCCCAGTGCATTATGTCTTGGTAGAGCCCTGAGGACACT
  GACAGTAGCATCTCTAAGTAAGTAGTGCTGTATGAATACAGACACATGCGGATCTGTATC
  TACATCCATCTGACTAGGCCAAGGAGCAGGTAGATGCAAGATAGAGACACACACATGCTG
  GATGGGGCCACTGCACACCTTGTCATGCCATTTAAAAGGGCAGTTACAGGTTGCCGGTTT
  TGCAGCCATTAAAATTACACTTTATGGAAAAGGCTTCTCTAATTGTGTCTGATTTGCTTT
  CTGTAGTAAGCATCATTGGAAGAAGACCAAAGCAAGAGCAACTAGAAGTTAAATATACAT
  TTGAGCTTAGCTGCAATACAGAACCCTGGGGAAGAAGCAGGCTGAAAGATTTATTTAATT
  TTAGGCTTGGATTCTCTCCCTTGCTGTTNTCTTGATGTTGTGTGTCTGCATTTGTGTGTG
  TGTGTGTGTCTGCGTGTGTGTGTGTTCCATCACATCATCTNCTGAAGAACGCTGGGTTTC
  GCAGGCAGGCGGCTAGTCACCTGGCACAACCCAGGGGTCCTGCCGAGGCTTCCAGGCTGC
  TGTCAGTGTAAAATGCCCAGGTTCTGGGCCGGTGACATGGAGGAGATGTTATTGACCGAC
  GAGGATGGAGGGGGACTTTCACTGCTGGTCACTGCTGCACCTGAAGAAAAAGCTGTCCTT
  TTACTACACCAATTTAGNTCTTTTTTCTGAAATAATACTTCCTCGGAGAAAAAGTCTTTC
  CTTCTTCAAACGGGGGGCCCCCTAATCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_138982
• Insert Size: 2620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138982.1, NP_620448.1
• RefSeq Size: 2211 bp
• RefSeq ORF: 1395 bp
• Locus ID: 5602
• Cytogenetics: 4q21.3
• Domains: pkinase
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, GnRH signaling pathway, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, Wnt signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system, and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017]'
  Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1, also known as JNK3 alpha2) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.