JNK3 (MAPK10) (NM_138982) Human Untagged Clone

CAT#: SC323665

MAPK10 (untagged)-Kinase deficient mutant (K93M) of Human mitogen-activated protein kinase 10 (MAPK10), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAPK10
• Synonyms: JNK3; JNK3A; p54bSAPK; p493F12; PRKM10; SAPK1b
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_138982, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCCTCCATTTCTTATACTACTGCAGTGAACCAACATTGGATGTGAAAATTGCCTTTTGTCAGGGAT
  TCGATAAACAAGTGGATGTGTCATATATTGCCAAACATTACAACATGAGCAAAAGCAAAGTTGACAACCA
  GTTCTACAGTGTGGAAGTGGGAGACTCAACCTTCACAGTTCTCAAGCGCTACCAGAATCTAAAGCCTATT
  GGCTCTGGGGCTCAGGGCATAGTTTGTGCCGCGTATGATGCTGTCCTTGACAGAAATGTGGCCATTAAGA
  AGCTCAGCAGACCCTTTCAGAACCAAACACATGCCAAGAGAGCGTACCGGGAGCTGGTCCTCATGAAGTG
  TGTGAACCATAAAAACATTATTAGTTTATTAAATGTCTTCACACCCCAGAAAACGCTGGAGGAGTTCCAA
  GATGTTTACTTAGTAATGGAACTGATGGATGCCAACTTATGTCAAGTGATTCAGATGGAATTAGACCATG
  AGCGAATGTCTTACCTGCTGTACCAAATGTTGTGTGGCATTAAGCACCTCCATTCTGCTGGAATTATTCA
  CAGGGATTTAAAACCAAGTAACATTGTAGTCAAGTCTGATTGCACATTGAAAATCCTGGACTTTGGACTG
  GCCAGGACAGCAGGCACAAGCTTCATGATGACTCCATATGTGGTGACACGTTATTACAGAGCCCCTGAGG
  TCATCCTGGGGATGGGCTACAAGGAGAACGTGGATATATGGTCTGTGGGATGCATTATGGGAGAAATGGT
  TCGCCACAAAATCCTCTTTCCAGGAAGGGACTATATTGACCAGTGGAATAAGGTAATTGAACAACTAGGA
  ACACCATGTCCAGAATTCATGAAGAAATTGCAACCCACAGTAAGAAACTATGTGGAGAATCGGCCCAAGT
  ATGCGGGACTCACCTTCCCCAAACTCTTCCCAGATTCCCTCTTCCCAGCGGACTCCGAGCACAATAAACT
  CAAAGCCAGCCAAGCCAGGGACTTGTTGTCAAAGATGCTAGTGATTGACCCAGCAAAAAGAATATCAGTG
  GACGACGCCTTACAGCATCCCTACATCAACGTCTGGTATGACCCAGCCGAAGTGGAGGCGCCTCCACCTC
  AGATATATGACAAGCAGTTGGATGAAAGAGAACACACAATTGAAGAATGGAAAGAACTTATCTACAAGGA
  AGTAATGAATTCAGAAGAAAAGACTAAAAATGGTGTAGTAAAAGGACAGCCTTCTCCTTCAGGTGCAGCA
  GTGAACAGCAGTGAGAGTCTCCCTCCATCCTCGTCTGTCAATGACATCTCCTCCATGTCCACCGACCAGA
  CCCTGGCATCTGACACTGACAGCAGCCTGGAAGCCTCGGCAGGACCCCTGGGTTGTTGCAGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_138982 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGCGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTTTCTTGGAAAT
  CACCTGTCCATCGTTAATTTTAAACAATCTCCATATCTCCAAAGAATCTCTTCCATGTTAGTCTGGAATG
  TGGTTAATGAAAAACAAGTAGGGAGGATTTCTGGGGCAAACACTGCCGGATCAGGATCGTAGTTCTCAGG
  CACGGAATGGCTAGTGTGAGAAACACCAACAGCAGGCCCCATCTCAGATCTTCACTATGGCAACTTATGC
  AAGAAACTGTTGAATTAGACCCGTTTCCTATAGATGAAAAACCATACCAAGCTGTGGTATTAATGAGCCT
  CCATTTCTTATACTACTGCAGTTGACCAACATTGATGGTGAAAATTGCCTTTTGTCAGGAATCGATAACA
  GTGGAGGTGTCATATATTGCAACATAAAACATGAGCAAAAGCAAAGTGACACCAGTTCCCGGGGGGAGGG
  GAAATCAACCTTCCCGTCCTAAGCCCTACCAGATCTAAGCCTATTGCTTCGGGCTCAAGGCATAAGTTGC
  GCGTATATATCCTCTTGAACAAATGGGCCATTAGAGCTAAGACCTTTCGACCACATGCGAGACGTACCGG
  ACGTGTCTTAGATGGGACCTAACGTAAGTTTAAGGTCCAACAGAACCGGATTCGAGTCATATAGACGAAG
  GACCATCCCGATCCCAGATTAACAGAAGATACCGACAGTGTTGGTGA
  
  Kinase domain sequence

  >SC323665 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  TGTATCTAGCCTATTGGCTCTGGGGCTCAGGGCATAGTTTGTGCCGCGTATGATGCTGTCCTTGACAGAA
  ATGTGGCCATTATGAAGCTCAGCAGACCCTTTCAGAACCAAACACATGCCAAGAGAGCGTACCGGGAGCT
  GGTCCTCATGAAGTGTGTGAACCATAAAAACATTATTAGTTTATTAAATGTCTTCACACCCCAGAAAACG
  CTGGAGGAGTTCCAAGATGTTTACTTAGTAATGGAACTGATGGAT
  
• Restriction Sites: Please inquire
• ACCN: NM_138982
• Insert Size: 2620 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138982.1, NP_620448.1
• RefSeq Size: 2211 bp
• RefSeq ORF: 1395 bp
• Locus ID: 5602
• Cytogenetics: 4q21.3
• Domains: pkinase
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Colorectal cancer, Epithelial cell signaling in Helicobacter pylori infection, ErbB signaling pathway, Fc epsilon RI signaling pathway, Focal adhesion, GnRH signaling pathway, Insulin signaling pathway, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, Wnt signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system, and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017]'
  Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1, also known as JNK3 alpha2) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.