PSMD10 (NM_170750) Human Untagged Clone

CAT#: SC109645

PSMD10 (untagged)-Human proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (PSMD10), transcript variant 2


  "NM_170750" in other vectors (4)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PSMD10
Synonyms dJ889N15.2; p28; p28(GANK)
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_170750, the custom clone sequence may differ by one or more nucleotides


ATGGAGGGGTGTGTGTCTAACCTAATGGTCTGCAACCTGGCCTACAGCGGGAAGCTGGAAGAGTTGAAGG
AGAGTATTCTGGCCGATAAATCCCTGGCTACTAGAACTGACCAGGACAGCAGAACTGCATTGCACTGGGC
ATGCTCAGCTGGACATACAGAAATTGTTGAATTTTTGTTGCAACTTGGAGTGCCAGTGAATGATAAAGAC
GATGCAGGTTGGTCTCCTCTTCATATTGCGGCTTCTGCTGGCCGGGATGAGATTGTAAAAGCCCTTCTGG
GAAAAGGTGCTCAAGTGAATGCTGTCAATCAAAATGGCTGTACTCCCTTACATTATGCAGCTTCGAAAAA
CAGGCATGAGATCGCTGTCATGTTACTGGAAGGCGGGGCTAATCCAGATGCTAAGGACCATTATGAGGCT
ACAGCAATGCACCGGGCAGCAGCCAAGGACACTTAG


>OriGene 5' read for NM_170750 unedited
AGTAATTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGAAAGGAGGGGT
GTGTGTCTAACCTAATGGTCTGCAACCTGGCCTACAGCGGGAAGCTGGAAGAGTTGAAGG
AGAGTATTCTGGCCGATAAATCCCTGGCTACTAGAACTGACCAGGACAGCAGAACTGCAT
TGCACTGGGCATGCTCAGCTGGACATACAGAAATTGTTGAATTTTTGTTGCAACTTGGAG
TGCCAGTGAATGATAAAGACGATGCAGGTTGGTCTCCTCTTCATATTGCGGCTTCTGCTG
GCCGGGATGAGATTGTAAAAGCCCTTCTGGGAAAAGGTGCTCAAGTGAATGCTGTCAATC
AAAATGGCTGTACTCCCTTACATTATGCAGCTTCGAAAAACAGGCATGAGATCGCTGTCA
TGTTACTGGAAGGCGGGGCTAATCCAGATGCTAAGGACCATTATGAGGCTACAGCAATGC
ACCGGGCAGCAGCCAAGGACACTTANCCTGTGATGAGGAGAGAGTGGAAGAAGCAAAACT
GCTGGTGTCCCAAGGAGCAAGTATTTACATTGAGAATAAAGAAGAAAAGACACCCCTGCA
AGTGGCCAAAGGTGGCCTGGGTTTAATACTCAAGAGAATGGTGNGAGGTTAAACAGCTTG
GATTTATTCTTACTNTGTATGTTGGGTTGTTGCCCCCAGTGTCCTACAAACTAATGTATT
TGTGCACAAGACATCATCTATGAATGATGAAAGTTTCTCACCTTCAAAGTCTTAAAACCA
TGTGACTCTTGTTCCTGCTGAGTTACTGGTTCGAGCCTACAGCTGTTTTCCAGCATCGAA
TAACTGTGAGATGTTCTCT
Restriction Sites NotI-NotI     
ACCN NM_170750
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_170750.1, NP_736606.1
RefSeq Size 1467 bp
RefSeq ORF 456 bp
Locus ID 5716
Cytogenetics Xq22.3
Gene Summary 'This gene encodes a subunit of the PA700/19S complex, which is the regulatory component of the 26S proteasome. The 26S proteosome complex is required for ubiquitin-dependent protein degradation. This protein is a non-ATPase subunit that may be involved in protein-protein interactions. Aberrant expression of this gene may paly a role in tumorigenesis. Two transcripts encoding different isoforms have been described. Pseudogenes have been identified on chromosomes 3 and 20.[provided by RefSeq, Mar 2011]'
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region which results in a frameshift, compared to variant 1. It encodes isoform 2 which has a shorter and distinct C-terminus, compared to isoform 1.

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