CD75 (ST6GAL1) (NM_003032) Human Untagged Clone

CAT#: SC109756

ST6GAL1 (untagged)-Human ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 (ST6GAL1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ST6GAL1
• Synonyms: SIAT1; ST6GalI; ST6N
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003032, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATTCACACCAACCTGAAGAAAAAGTTCAGCTGCTGCGTCCTGGTCTTTCTTCTGTTTGCAGTCATCT
  GTGTGTGGAAGGAAAAGAAGAAAGGGAGTTACTATGATTCCTTTAAATTGCAAACCAAGGAATTCCAGGT
  GTTAAAGAGTCTGGGGAAATTGGCCATGGGGTCTGATTCCCAGTCTGTATCCTCAAGCAGCACCCAGGAC
  CCCCACAGGGGCCGCCAGACCCTCGGCAGTCTCAGAGGCCTAGCCAAGGCCAAACCAGAGGCCTCCTTCC
  AGGTGTGGAACAAGGACAGCTCTTCCAAAAACCTTATCCCTAGGCTGCAAAAGATCTGGAAGAATTACCT
  AAGCATGAACAAGTACAAAGTGTCCTACAAGGGGCCAGGACCAGGCATCAAGTTCAGTGCAGAGGCCCTG
  CGCTGCCACCTCCGGGACCATGTGAATGTATCCATGGTAGAGGTCACAGATTTTCCCTTCAATACCTCTG
  AATGGGAGGGTTATCTGCCCAAGGAGAGCATTAGGACCAAGGCTGGGCCTTGGGGCAGGTGTGCTGTTGT
  GTCGTCAGCGGGATCTCTGAAGTCCTCCCAACTAGGCAGAGAAATCGATGATCATGACGCAGTCCTGAGG
  TTTAATGGGGCACCCACAGCCAACTTCCAACAAGATGTGGGCACAAAAACTACCATTCGCCTGATGAACT
  CTCAGTTGGTTACCACAGAGAAGCGCTTCCTCAAAGACAGTTTGTACAATGAAGGAATCCTAATTGTATG
  GGACCCATCTGTATACCACTCAGATATCCCAAAGTGGTACCAGAATCCGGATTATAATTTCTTTAACAAC
  TACAAGACTTATCGTAAGCTGCACCCCAATCAGCCCTTTTACATCCTCAAGCCCCAGATGCCTTGGGAGC
  TATGGGACATTCTTCAAGAAATCTCCCCAGAAGAGATTCAGCCAAACCCCCCATCCTCTGGGATGCTTGG
  TATCATCATCATGATGACGCTGTGTGACCAGGTGGATATTTATGAGTTCCTCCCATCCAAGCGCAAGACT
  GACGTGTGCTACTACTACCAGAAGTTCTTCGATAGTGCCTGCACGATGGGTGCCTACCACCCGCTGCTCT
  ATGAGAAGAATTTGGTGAAGCATCTCAACCAGGGCACAGATGAGGACATCTACCTGCTTGGAAAAGCCAC
  ACTGCCTGGCTTCCGGACCATTCACTGCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003032 unedited
  CCCCCATTTCCCCCCCGTTGCCGCTTTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATAT
  AAGCAGTTTTCATTTACGTGACACTATAGGGGACAGGCTACTTGTTCTTTATGCAGCGGC
  CGCGAATTCGGCACGAGGATAAACCTTCCCTCCCATGGATAATAGTGCTAATTCCTGAGG
  ACCTGAAGGGCCTGCCGCCCCTGGGGGATTAGCCAGAAGCAGGCTTGTTTTCCTGCTCAG
  AACAAAGGGAGTTCCCTGAACACATCTTCATTATGATTCACACCAACCTGAAGAAAAAGT
  TCAGCTGCTGCGTCCTGGTCTTTCTTCTGTTTGCAGTCATCTGTGTGTGGAAGGAAAAGA
  AGAAAGGGAGTTACTATGATTCCTTTAAATTGCAAACCAAGGAATTCCAGGTGTTAAAGA
  GTCTGGGGAAATTGGCCATGGGGTCTGATTCCCAGTCTGTATCCTCAAGCAGCACCCAGG
  ACCCCCACAGGGGCCGCCAGACCCTCGGCAGTCTCAGAGGCCTAGCCAAGGCCAAACCAG
  AGGCCTCCTTCCAGGTGTGGAACAAGGACAGCTCTTCCAAAAACCTTATCCCTAGGCTGC
  AAAAGATCTGGAAGAATTACCTAAGCATGAACAAGTACAAAGTGTCCTACAAGGGGCCAG
  GACCAGGCATCAAGTTCAGTGCAGAGGCCCTGCGCTGCCACCTCCGGGACCATGTGAATG
  TATCCATGGTAGAGGTCACAGATTTTCCCTTCAATACCTCTGCATGGGAGGGTTATCTGC
  TCAAGGAGAGCATTAGGACCAAGGCTGGGCCTTGGGGCACGTGTGCTGTTGTGTCGTCAG
  CGGGATCTCTTGAAGTCTNCCTACTAGGCAGAGACATCGATGATCTGGACGCAGTCCTGA
  GGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003032 unedited
  GTTTACCAGAAATACTATGNACCGCGCCGCTTTCTANNGATCGGTTTTTTTTTTTTTTTT
  TTAAAGAACACACACACATTTATTTTATAATTTGACATAATCCTTGGGTAATTATTAGCA
  TTTTTCAGAAGTTTCCGAGACTGATATCTCAACTCCAAACCAATAGCAATAGCTTATTTG
  ATTGGTGGAAGGATTAGAGAGGGTCCCATGATCCAGATGAATAGATGGCTATCAAAGAAG
  ACAGAACCCCATAGACACAGGCAGCATTAAGTCATAATTTTTAAATTAACTGTCACAAAT
  ATGAAACATCTCACCAGACCAATGATAACGTCCATATCATAATTTGCATTTTACCAAAGT
  GTCCACATAGACAGCACAGAACTAAAAGTTCTGTAGACTTCAGAATCATATTTAAATTAA
  TTTGATCCTTTATATTAATAATAATTAAAAACTAGATTCTACCCAGTGTCGTCCCAGTCT
  TCACACCAACATGTATGTCCTGCTGTCGGAATGCCACCTCCTCTGCACTCAGTGTCATGA
  TTCGAAGATTAAAATCAGTGTGTCAAATCAGAACATAAACCAAGCCAGGAATTCATGGGC
  CCAGAGAGGGGTAAGGGGCTTGTCCTATACAGCAGTTAAGCAGCCCACGTGCTCCGCCCA
  TTCCCAAGCAGAATCAGATTGGAGCTGCANGCANCGAGGAGGAGGGCGTGTTCTGTGTTG
  TCCACTGCTTTGCATGGTTTCTTGGGTGTTCCAAGGAGGACAGTTGTTGTCTCCCATAAG
  ACAGTTAATTCCCTGAGGACGGACCTCTTNCAACTAGCCTNCGTCACAGCAGGGTGCATG
  CAGNCCGAGTGTGAGCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003032
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003032.2, NP_003023.1
• RefSeq Size: 4295 bp
• RefSeq ORF: 1221 bp
• Locus ID: 6480
• Cytogenetics: 3q27.3
• Domains: Glyco_transf_29
• Protein Families: Secreted Protein
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. The promoter and 5' terminal exon sequence is from an endogenous retroviral LTR (PMID: 8724135). Variant 2 is expressed in mature B lymphocytes and variants 1, 2 and 4 encode the same protein (isoform a).