UBE2I (NM_003345) Human Untagged Clone

CAT#: SC109867

UBE2I (untagged)-Human ubiquitin-conjugating enzyme E2I (UBE2I), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBE2I
• Synonyms: C358B7.1; P18; UBC9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109867 sequence for NM_003345 edited (data generated by NextGen Sequencing)
  ATGTCGGGGATCGCCCTCAGCAGACTCGCCCAGGAGAGGAAAGCATGGAGGAAAGACCAC
  CCATTTGGTTTCGTGGCTGTCCCAACAAAAAATCCCGATGGCACGATGAACCTCATGAAC
  TGGGAGTGCGCCATTCCAGGAAAGAAAGGGACTCCGTGGGAAGGAGGCTTGTTTAAACTA
  CGGATGCTTTTCAAAGATGATTATCCATCTTCGCCACCAAAATGTAAATTCGAACCACCA
  TTATTTCACCCGAATGTGTACCCTTCGGGGACAGTGTGCCTGTCCATCTTAGAGGAGGAC
  AAGGACTGGAGGCCAGCCATCACAATCAAACAGATCCTATTAGGAATACAGGAACTTCTA
  AATGAACCAAATATCCAAGACCCAGCTCAAGCAGAGGCCTACACGATTTACTGCCAAAAC
  AGAGTGGAGTACGAGAAAAGGGTCCGAGCACAAGCCAAGAAGTTTGCGCCCTCATAA
  
  Clone variation with respect to NM_003345.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003345 unedited
  GTTCGAATTTGTATACGACTCCTATAGGCGGCCCGCGATTCGGCACGAGGGCGGGCTCCG
  GAGGGAAGTCCCGAGACAAAGGGAAGCGCCGCCGCCGCCGCCCCGCTCGGTCCTCCACCT
  GTCCGCTACGCTCGCCGGGGCTGCGGCCGCCCGAGGGACTTTGAACATGTCGGGGATCGC
  CCTCAGCAGACTCGCCCAGGAGAGGAAAGCATGGAGGAAAGACCACCCATTTGGTTTCGT
  GGCTGTCCCAACAAAAAATCCCGATGGCACGATGAACCTCATGAACTGGGAGTGCGCCAT
  TCCGGGAAAGAAAGGGACTCCGTGGGAAGGAGGCTTGTTTAAACTACGGATGCTTTTCAA
  AGATGATTATCCATCTTCGCCACCAAAATGTAAATTCGAACCACCATTATTTCACCCGAA
  TGTGTACCCTTCGGGGACAGTGTGCCTGTCCATCTTAGAGGAGGACAAGGACTGGAGGCC
  AGCCATCACAATCAAACAGATCCTATTAGGAATACAGGAACTTCTAAATGAACCAAATAT
  CCAAGACCCAGCTCAAGCAGAGGCCTACACGATTTACTGCCAAAACAGAGTGGAGTACGA
  GAAAAGGGTCCGAGCACAAGCCAAGAAGTTTGCGCCCTCATAAGCAGCGACCTTGTGGCA
  TCGTCAAAAGGAAGGGATTGGTTTGGCAAGAACTTGTTTACAACATTNTTGCANATCTAA
  AGTTGCTCCATACAATGACTAGTCACCTGGGGGGGGNTGGGGCGGGGCGCCATCTTCCAT
  TGCCGCCGCGGNNTGTGCGGTCTCGATTCGCTGAATTGCCCGTTTCCATACAGGGNNCTC
  TCCTTCGGNCTTNGNATTTTGGATGGGTATGGNAAACTCGCTNNTATTTATTAATGGATG
  GCAGNATTTCAACTGCTGTANAATATAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003345 unedited
  AATCCTGNNACCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTACCTTTAT
  ATAAACAGTTTATTTACTCTAAACAGCAGCGCAGACAAAACGCCATATAAACACAAAGGA
  AGTGGCGCAGAACCGAGATGCTGCCCTGGGGTCTGAGGCTCAAAAGCAGCACAGCCCAGG
  TTCTAGAAGGTTCTAGAGAGGAGCGGCAGGAGGGAGGGTGCATTTCCATACAGCCAGCTG
  AGGCCAAGTGCAGCTCTGCACGGTGAGAAGCATGCCTGCATCCCAGAGCGAGGAACTCGC
  CCCTGGGGGACTTACCCAAGTATAAAAGTTTATAATTTTACAGCAGTTGAAATACTGACA
  TCAATATTAAAATAAAAGCGAGTTTTACATAACAATCAAAAATACAAAAGACCGAAGGAA
  GAGACCCTGTATGGAAACGGGCAATTCAGCGAATCGAGACCGCACACCCGCGGCGGCAAT
  GGAAGATGGCGCCCGCCCAACCCCCCCAGGTGACTAGTCATTGTATGGAGCAACTTTAGA
  TTTGCAAAAATGTTGTAAACAAGTTCTTGCCAAACCAATCCCTTCCTTTTGACGATGCCA
  CAAGGGTCGCTGCTTATGAGGGCGCAAACTTCTTGGCTTGTGCTCGGACCCTTTTCTCGT
  ACTCCACTCTGNTTGGGAGTAAATCGTGTAAGCCTCTGCTTGACCTGGGTCTTGGATATT
  TGGTTCATTTAAAAGTCCTGGATCCCAATAAGATCCGTTTGAATGGAATGGCTGCCCTCC
  AGTCCTTGGCCTCCTCTTAAAGGACCAGGCCCCTGTCCCCCAGGTTACACATTCGGGGGA
  AAAAAGGTGGGGTCACATTTACCTTTGGGGGCCAAGATAGGATATTATTTTGAAAAGCCT
  CGCGTTTAACCAACCCCCTTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003345
• Insert Size: 1080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003345.3, NP_003336.1
• RefSeq Size: 1221 bp
• RefSeq ORF: 477 bp
• Locus ID: 7329
• Cytogenetics: 16p13.3
• Domains: UBCc
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the predominant transcript. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.