BHMT (NM_001713) Human Untagged Clone

CAT#: SC110837

BHMT (untagged)-Human betaine--homocysteine S-methyltransferase (BHMT)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BHMT
• Synonyms: BHMT1; HEL-S-61p
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001713, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCACCCGTTGGGGGCAAAAAGGCCAAGAAGGGCATCCTAGAACGTTTAAATGCTGGAGAGATTGTGA
  TTGGAGATGGAGGGTTTGTCTTTGCACTGGAGAAGAGGGGCTACGTAAAGGCAGGACCCTGGACTCCTGA
  AGCTGCTGTGGAGCACCCAGAAGCAGTTCGCCAGCTTCATCGAGAGTTCCTCAGAGCTGGCTCAAACGTC
  ATGCAGACCTTCACCTTCTATGCGAGTGAAGACAAGCTGGAGAACAGGGGCAACTATGTCTTAGAGAAGA
  TATCTGGGCAGGAAGTCAATGAAGCTGCTTGCGACATCGCCCGACAAGTGGCTGATGAAGGAGATGCTTT
  GGTAGCAGGAGGAGTGAGTCAGACACCTTCATACCTTAGCTGCAAGAGTGAAACTGAAGTCAAAAAAGTA
  TTTCTGCAACAGTTAGAGGTCTTTATGAAGAAGAACGTGGACTTCTTGATTGCAGAGTATTTTGAACACG
  TTGAAGAAGCTGTGTGGGCAGTTGAAACCTTGATAGCATCCGGTAAACCTGTGGCAGCAACCATGTGCAT
  TGGCCCAGAAGGAGATTTGCATGGCGTGCCCCCCGGCGAGTGTGCAGTGCGCCTGGTGAAAGCAGGAGCA
  TCCATCATTGGTGTGAACTGCCACTTTGACCCCACCATTAGTTTAAAAACAGTGAAGCTCATGAAGGAGG
  GCTTGGAGGCTGCCCGACTGAAAGCTCACCTGATGAGCCAGCCCTTGGCTTACCACACTCCTGACTGCAA
  CAAGCAGGGATTCATCGATCTCCCAGAATTCCCATTTGGACTGGAACCCAGAGTTGCCACCAGATGGGAT
  ATTCAAAAATACGCCAGAGAGGCCTACAACCTGGGGGTCAGGTACATTGGCGGGTGCTGTGGATTTGAGC
  CCTACCACATCAGGGCAATTGCAGAGGAGCTGGCCCCAGAAAGGGGCTTTTTGCCACCAGCTTCAGAAAA
  ACATGGCAGCTGGGGAAGTGGTTTGGACATGCACACCAAACCCTGGGTTAGAGCAAGGGCCAGGAAGGAA
  TACTGGGAGAATCTTCGGATAGCCTCAGGCCGGCCATACAACCCTTCAATGTCAAAGCCAGATGGCTGGG
  GAGTGACCAAAGGAACAGCCGAGCTGATGCAGCAGAAAGAAGCCACAACTGAGCAGCAGCTGAAAGAGCT
  CTTTGAAAAACAAAAATTCAAATCACAGTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001713 unedited
  GTTCACATTTGTATACGACTCCTATAGGCGGCNCGCGAATTCGCACCAGGGGAAGGCTCG
  CCTAGTCGGTCCGCATCCGTGTCGACCACCTGTCTGGACACCACGAAGATGCCACCCGTT
  GGGGGCAAAAAGGCCAAGAAGGGCATCCTAGAACGTTTAAATGCTGGAGAGATTGTGATT
  GGAGATGGAGGGTTTGTCTTTGCACTGGAGAAGAGGGGCTACGTAAAGGCAGGACCCTGG
  ACTCCTGAAGCTGCTGTGGAGCACCCAGAAGCAGTTCGCCAGCTTCATCGAGAGTTCCTC
  AGAGCTGGCTCAAACGTCATGCAGACCTTCACCTTCTATGCGAGTGAAGACAAGCTGGAG
  AACAGGGGCAACTATGTCTTAGAGAAGATATCTGGGCAGGAAGTCAATGAAGCTGCTTGC
  GACATCGCCCGACAAGTGGCTGATGAAGGAGATGCTTTGGTAGCAGGAGGAGTGAGTCAG
  ACACCTTCATACCTTAGCTGCAAGAGTGAAACTGAAGTCAAAAAAGTATTTCTGCAACAG
  TTAGAGGTCTTTATGAAGAAGAACGTGGACTTCTTGATTGCAGAGTATTTTGAACACGTT
  GAAGAAGCTGTGTGGGCAGTTGAAACCTTGATAGCATCCGGTAAACCTGTGGCAGCAACC
  ATGTGCATTGGCCCAGAAGGAGATTTGCATGGCGTGCCCCCCGGCGAGTGTGCAGTGCGC
  CTGGTGAAAGCANGAGCATTCATCATTGGTGTGAACTGCCACTTTGACCCCACATTAGTT
  TANAACCAGTGAACTCATGAAAGGAGGCTTGGNAGCTTCCCCACTGAAAGCTCACCTGAT
  GAGCCAGCCCTTGGCTCACACACTTCTGACTGCACAAGCAGGGATTCATCGATCTCCCAA
  ATTCCCATTTGGACTGGAACCCAGAGATG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001713 unedited
  NTTATCTTGCCCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTCTCATTG
  CTCTTCTTTTTAATAGCTGAAAAGATTACAATTTCAAAACAGCTCAAACAATTCAATAAT
  GTTTAGAAAGCTGCATGACCTCTTTTCTAATACACTGAGGGTAGTCATGTTTCTTCTAAT
  AGTCTTGTATCTTCAGGCTTGAGCTTTTGTAGGAGAGTAGATATTAAATTATCAGAGAAA
  AATATCATAGTTCTCAATCTAGGAAACCAGGCTAGAGTTTGGAGAATCTCTACTTTTTTC
  TTATTATATTTTTTAAAAGCTACCAACTAATACAAGCTCACTCTGCTCAGCCTTATGGCC
  CAGAGGCACTAGGTGGATCCAGTGTTGACTAGGATGGAGAGGGCTGAAATATCCATCTCT
  CCTCCCCTCCTCCCCATTGGTCTATGTCTTTTACTGGTAGAAAAGTGGGTCACGAAACAG
  AAGAACTCTGAGAGCAGGACTCTGGGTATTGGATATTTATTGATCAGGTCTCATATGACT
  GACCTACTTATAAGAATAAACTTAAACAGGATGGTCTCCTTAAGTGTCTTGTAGCAATAT
  TTTTCAAACTGTGGATCCAAACCCAGTAGTGGGTCATAAAAATCCATTTGAAAAGTCTCA
  ACTAGCATGTTTTATAAATGAAATAGAATGAAAAGGAATGGAACAGAAAATACTAGGATG
  GATCACACATTTAATTTCGACAGTAAGATGTGATGTGTTTTGCTGGGTCATTATATAAAT
  GNNGTTGTACTAANGGGTCATGTCAAAGAACTTGGAGCCCCTTNCATAGCGCTTATCTGT
  CCTGGCTGGTGCTGAGTCTTAAGACAGTCATGTCTATCCTCTTGCTAGCTACCACTTCTT
  CAAGTGCATTACATCTGAATACTTTTCCTGTGCTGCAGNGATTTCATATACTTTCCAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001713
• Insert Size: 2570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001713.1, NP_001704.1
• RefSeq Size: 2436 bp
• RefSeq ORF: 1221 bp
• Locus ID: 635
• Cytogenetics: 5q14.1
• Domains: S-methyl_trans
• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]'