Decorin (DCN) (NM_001920) Human Untagged Clone

CAT#: SC110867

DCN (untagged)-Human decorin (DCN), transcript variant A1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCN
• Synonyms: CSCD; DSPG2; PG40; PGII; PGS2; SLRR1B
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001920, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGGCCACTATCATCCTCCTTCTGCTTGCACAAGTTTCCTGGGCTGGACCGTTTCAACAGAGAGGCT
  TATTTGACTTTATGCTAGAAGATGAGGCTTCTGGGATAGGCCCAGAAGTTCCTGATGACCGCGACTTCGA
  GCCCTCCCTAGGCCCAGTGTGCCCCTTCCGCTGTCAATGCCATCTTCGAGTGGTCCAGTGTTCTGATTTG
  GGTCTGGACAAAGTGCCAAAGGATCTTCCCCCTGACACAACTCTGCTAGACCTGCAAAACAACAAAATAA
  CCGAAATCAAAGATGGAGACTTTAAGAACCTGAAGAACCTTCACGCATTGATTCTTGTCAACAATAAAAT
  TAGCAAAGTTAGTCCTGGAGCATTTACACCTTTGGTGAAGTTGGAACGACTTTATCTGTCCAAGAATCAG
  CTGAAGGAATTGCCAGAAAAAATGCCCAAAACTCTTCAGGAGCTGCGTGCCCATGAGAATGAGATCACCA
  AAGTGCGAAAAGTTACTTTCAATGGACTGAACCAGATGATTGTCATAGAACTGGGCACCAATCCGCTGAA
  GAGCTCAGGAATTGAAAATGGGGCTTTCCAGGGAATGAAGAAGCTCTCCTACATCCGCATTGCTGATACC
  AATATCACCAGCATTCCTCAAGGTCTTCCTCCTTCCCTTACGGAATTACATCTTGATGGCAACAAAATCA
  GCAGAGTTGATGCAGCTAGCCTGAAAGGACTGAATAATTTGGCTAAGTTGGGATTGAGTTTCAACAGCAT
  CTCTGCTGTTGACAATGGCTCTCTGGCCAACACGCCTCATCTGAGGGAGCTTCACTTGGACAACAACAAG
  CTTACCAGAGTACCTGGTGGGCTGGCAGAGCATAAGTACATCCAGGTTGTCTACCTTCATAACAACAATA
  TCTCTGTAGTTGGATCAAGTGACTTCTGCCCACCTGGACACAACACCAAAAAGGCTTCTTATTCGGGTGT
  GAGTCTTTTCAGCAACCCGGTCCAGTACTGGGAGATACAGCCATCCACCTTCAGATGTGTCTACGTGCGC
  TCTGCCATTCAACTCGGAAACTATAAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001920 unedited
  CGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAG
  GGAAGCAGGAGGTTTTCAACCTAGTCACAGAGCAGCACCTACCCCCTCCTCCTTTCCACA
  CCTGCAAACTCTTTTACTTGGGCTGAATATTTAGTGTAATTACATCTCAGCTTTGAGGGC
  TCCTGTGGCAAATTCCCGGATTAAAAGGTTCCCTGGTTGTGAAAATACATGAGATAAATC
  ATGAAGGCCACTATCATCCTCCTTCTGCTTGCACAAGTTTCCTGGGCTGGACCGTTTCAA
  CAGAACAGGCTTATTTGACTTTATGCTAGAAGATGAGGCTTCTGGGATAGGCCCAGAAGT
  TCCTGATGACCGCGACTTCGAGCCCTCCCTAGGCCCAGTGTGCCCCTTCCGCTGTCAATG
  CCATCTTCGAGTGGTCCAGTGTTCTGATTTGGGTCTGGACAAAGTGCCAAAGGATCTTCC
  CCCTGACACAACTCTGCTAGACCTGCAAAACAACAAAATAACCGAAATCAAAGATGGAGA
  CTTTAAGAACCTGAAGAACCTTCACGCATTGATTCTTGTCAACAATAAAATTAGCAAAGT
  TAGTCCTGGAGCATTTACACCTTTGGTGAAGTTGGAACGACTNTATCTGTCCAAGAATCA
  GCTGAAGGAATTGCCAGAAAAAATGCCCANNAACTCTTCANGAGCTGCGTGCCCATGAGA
  ATGAGATCACCAAAGTGCGAAAAGTTACTTTCAATGGACTGAACCAGATGATTGTCATAG
  AACTGGGCACCAATCCGCTGAAGAGCTCANGAATTGANAATGGGGGCTTTCCAGNGAATG
  AAGAAGNCTCTCCTACATTCCGCATTGCTGATACCAATATCACCAGCATTCCTCAAGGNN
  CTTCTCCTTNCCTTAGGNNNATACATCTGATGGGCACAATCANCANAGTGATGCANCTAG
  CTGAAGGACTNNGATATTNGCTAAGTGGGATGAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001920 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATTTTGAATGTATTTTT
  AAATTTATTTTTTCAAAATAATGACATTAGTAAAAATTTTACATAGCCTGTATTGAATTC
  ACACATTCAAATGAGGCTTTACCAGTAATGATGGGGATTAATACAGAGCTAGTGTTTGGC
  ATTTGACTTTATCTCAAATGAGCTAACTGCTCAATGAATTACAGAAGACTCATACTCTTT
  TTATTTTTTCCTGGAAATTAAAAAAGAAAAGCTTTACTAAATATTGACATATATATTTAC
  TCCAAATTTTACATTTAGTGAAATAAGAATATCTCTAGTAGCTCAGTTAACATCAACAGA
  AAGCTTCAAAAGATGATTCTGAAAATGGCAGGCAAAATTTCTTTTTATTGTAGGCAATTA
  CTTAAACTGGAAATTTGGCTTTATGCATAATAAGTCATGTGGGTAAAACATCCACATTGC
  AGTTAGGTTTCCAGTATCTAGCTTTTATTTATTTTTTAGCAATGACATTAACAAGATTTT
  GCCAGGTTATAAAAATGAGGGCTTTCTTGAGAATTACTTATAGTTTCCGAGTTGAATGGC
  AGAGCGCACGTAGACACATCTGAAGGTGGATGGCTGTATCTCCCAGTACTGGACCGGGTT
  GCTGAAAAGACTCACACCCGAATAAGAAGCCTTTNTGGNTGTTGTGTCCCAGTGGGCAGA
  AGTCACTTGATCCCACTACAGAGATATTGNTGNTATGAANGTAGACAACCTGNATGTACT
  TATGCTCTGCCAGCCCACCCAGTACTCTGGTAAAGCTGTTGTGTCCAAGTGAAGCTCCTC
  AATGAAGCGTGTTGCCAGNAAGCATNGTCACAGCAGAGATGCTGTGAACTCATCCNACTA
  GCCAANTATCAGTCCTTCAGCTACTGCATCACTTGCTGATTTGTGCATCAGATGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001920
• Insert Size: 1770 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001920.3, NP_001911.1
• RefSeq Size: 2305 bp
• RefSeq ORF: 1080 bp
• Locus ID: 1634
• Cytogenetics: 12q21.33
• Domains: LRRNT, LRR, LRR_TYP, LRR_BAC, LRR_PS
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: 'This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients. [provided by RefSeq, Nov 2015]'
  Transcript Variant: This variant (A1) represents the longest transcript and encodes the longest isoform. Both variants A1 and A2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.