HIF 2 alpha (EPAS1) (NM_001430) Human Untagged Clone

CAT#: SC110883

EPAS1 (untagged)-Human endothelial PAS domain protein 1 (EPAS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EPAS1
• Synonyms: bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001430 edited
  CCCGCCCGGGCCGCGGGGAGCGGACGAGGGCCACAGCCCCCCACCCGCCAGGGAGCCCAG
  GTGCTCGGCGTCTGAACGTCTCAAAGGGCCACAGCGACAATGACAGCTGACAAGGAGAAG
  AAAAGGAGTAGCTCGGAGAGGAGGAAGGAGAAGTCCCGGGATGCTGCGCGGTGCCGGCGG
  AGCAAGGAGACGGAGGTGTTCTATGAGCTGGCCCATGAGCTGCCTCTGCCCCACAGTGTG
  AGCTCCCATCTGGACAAGGCCTCCATCATGCGACTGGCAATCAGCTTCCTGCGAACACAC
  AAGCTCCTCTCCTCAGTTTGCTCTGAAAACGAGTCCGAAGCCGAAGCTGACCAGCAGATG
  GATAACTTGTACCTGAAAGCCTTGGAGGGTTTCATTGCCGTGGTGACCCAAGATGGCGAC
  ATGATCTTTCTGTCAGAAAACATCAGCAAGTTCATGGGACTTACACAGGTGGAGCTAACA
  GGACATAGTATCTTTGACTTCACTCATCCCTGCGACCATGAGGAGATTCGTGAGAACCTG
  AGTCTCAAAAATGGCTCTGGTTTTGGGAAAAAAAGCAAAGACATGTCCACAGAGCGGGAC
  TTCTTCATGAGGATGAAGTGCACGGTCACCAACAGAGGCCGTACTGTCAACCTCAAGTCA
  GCCACCTGGAAGGTCTTGCACTGCACGGGCCAGGTGAAAGTCTACAACAACTGCCCTCCT
  CACAATAGTCTGTGTGGCTACAAGGAGCCCCTGCTGTCCTGCCTCATCATCATGTGTGAA
  CCAATCCAGCACCCATCCCACATGGACATCCCCCTGGATAGCAAGACCTTCCTGAGCCGC
  CACAGCATGGACATGAAGTTCACCTACTGTGATGACAGAATCACAGAACTGATTGGTTAC
  CACCCTGAGGAGCTGCTTGGCCGCTCAGCCTATGAATTCTACCATGCGCTAGACTCCGAG
  AACATGACCAAGAGTCACCAGAACTTGTGCACCAAGGGTCAGGTAGTAAGTGGCCAGTAC
  CGGATGCTCGCAAAGCATGGGGGCTACGTGTGGCTGGAGACCCAGGGGACGGTCATCTAC
  AACCCTCGCAACCTGCAGCCCCAGTGCATCATGTGTGTCAACTACGTCCTGAGTGAGATT
  GAGAAGAATGACGTGGTGTTCTCCATGGACCAGACTGAATCCCTGTTCAAGCCCCACCTG
  ATGGCCATGAACAGCATCTTTGATAGCAGTGGCAAGGGGGCTGTGTCTGAGAAGAGTAAC
  TTCCTATTCACCAAGCTAAAGGAGGAGCCCGAGGAGCTGGCCCAGCTGGCTCCCACCCCA
  GGAGACGCCATCATCTCTCTGGATTTCGGGAATCAGAACTTCGAGGAGTCCTCAGCCTAT
  GGCAAGGCCATCCTGCCCCCGAGCCAGCCATGGGCCACGGAGTTGAGGAGCCACAGCACC
  CAGAGCGAGGCTGGGAGCCTGCCTGCCTTCACCGTGCCCCAGGCAGCTGCCCCGGGCAGC
  ACCACCCCCAGTGCCACCAGCAGCAGCAGCAGCTGCTCCACGCCCAATAGCCCTGAAGAC
  TATTACACATCTTTGGATAACGACCTGAAGATTGAAGTGATTGAGAAGCTCTTCGCCATG
  GACACAGAGGCCAAGGACCAATGCAGTACCCAGACGGATTTCAATGAGCTGGACTTGGAG
  ACACTGGCACCCTATATCCCCATGGACGGGGAAGACTTCCAGCTAAGCCCCATCTGCCCC
  GAGGAGCGGCTCTTGGCGGAGAACCCACAGTCCACCCCCCAGCACTGCTTCAGTGCCATG
  ACAAACATCTTCCAGCCACTGGCCCCTGTAGCCCCGCACAGTCCCTTCCTCCTGGACAAG
  TTTCAGCAGCAGCTGGAGAGCAAGAAGACAGAGCCCGAGCACCGGCCCATGTCCTCCATC
  TTCTTTGATGCCGGAAGCAAAGCATCCCTGCCACCGTGCTGTGGCCAGGCCAGCACCCCT
  CTCTCTTCCATGGGGGGCAGATCCAATACCCAGTGGCCCCCAGATCCACCATTACATTTT
  GGGCCCACAAAGTGGGCCGTCGGGGATCAGCGCACAGAGTTCTTGGGAGCAGCGCCGTTG
  GGGCCCCCTGTCTCTCCACCCCATGTCTCCACCTTCAAGACAAGGTCTGCAAAGGGTTTT
  GGGGCTCGAGGCCCAGACGTGCTGAGTCCGGCCATGGTAGCCCTCTCCAACAAGCTGAAG
  CTGAAGCGACAGCTGGAGTATGAAGAGCAAGCCTTCCAGGACCTGAGCGGGGGGGACCCA
  CCTGGTGGCAGCACCTCACATTTGATGTGGAAACGGATGAAGAACCTCAGGGGTGGGAGC
  TGCCCTTTGATGCCGGACAAGCCACTGAGCGCAAATGTACCCAATGATAAGTTCACCCAA
  AACCCCATGAGGGGCCTGGGCCATCCCCTGAGACATCTGCCGCTGCCACAGCCTCCATCT
  GCCATCAGTCCCGGGGAGAACAGCAAGAGCAGGTTCCCCCCACAGTGCTACGCCACCCAG
  TACCAGGACTACAGCCTGTCGTCAGCCCACAAGGTGTCAGGCATGGCAAGCCGGCTGCTC
  GGGCCCTCATTTGAGTCCTACCTGCTGCCCGAACTGACCAGATATGACTGTGAGGTGAAC
  GTGCCCGTGCTGGGAAGCTCCACGCTCCTGCAAGGAGGGGACCTCCTCAGAGCCCTGGAC
  CAGGCCACCTGAGCCAGGCCTTCTACCTGGGCAGCACCTCTGCCGACGCCGTCCCACCAG
  CTTCACTCTCTCCGTCTGTTTTTGCAACTAGGTATTTCTAACGCCAGCACAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001430 unedited
  CCCAATCGCCGCCCGTTGCCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA
  GCAGAGTTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG
  CGAATTCGGCACGAGGGCCACACGGGTCCGGTGCCCGCTGCGCTTCCGCCCCAGCGCTCC
  TGAGGCGGCCGTACAATCCTCGGCAGTGTCCTGAGACTGTATGGTCAGCTCAGCCCGGCC
  TCCGACTCCTTCCGACTCCCAGCATTCGAGCCACTTTTTTTTTCTTTGAAAACTCAGAAA
  AGTGACTCCTTTTCCAGGGAAAAAGGAACTTGGGTTCCCTTCTCTCCGTCCTCTTTTCGG
  GTCTGACAGCCTCCACCCACTCCTTCCCCGGACCCCGCCTCCGCGCGCAGGTTCCTCCCA
  GTCACCTTTCTCCACCCCCGCCCCCGCACCTAGCCCGCCGCGCGCCACCTTCCACCTGAC
  TGCGCGGGGCGCTCGGGACCTGCGCGCACCTCGGACCTTCACCACCCGCCCGGGCCGCGG
  GGAGCGGACGAGGGCCACAGCCCCCCACCCGCCAGGGAGCCCAGGTGCTCGGCGTCTGAA
  CGTCTCAAAGGGCCACAGCGACAATGACAGCTGACCAGGAGAAGACAAGGAGTAGCTCGG
  AGAGGTAGAAGGAGAAGTCCCTGGATGCTGCGCGGTGCCTGCCGGAGCAGGAGACGGAGG
  TGTTCTATGAGCTGGCCCATGAGCTGCCTCTGCCCCACAGTGTGAGCTCCCATCTGGACC
  AGGTCTCCATCATGCGACTGGCAATCAGTTTCCTGGGACACACAGCTCCTCTCCTCAGAT
  TGCTCTGAAAACGAGTCCGAAGCCCCAGCTGACCACCAGAGGGATAACTTGTACCTGAAG
  CCTTTGAAGGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001430 unedited
  CACTGGGGGCAGGGGTACACAGGGCATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGA
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGGATCAAGATACTTTAA
  TAAGATACCAGTGTCAAAATACATTTCCTTATAAAGTTAAGCTCCCATACAGTTATAATG
  TTGTCTGTTGGAATTCGACAATATAATAACGTTCATGAAATCGTTACGTTGACAGGTAGG
  GTTAATATGAAGCTTGGAATATTTTTCAGTGTTTTAGTAAAACTGCAAGGGTAAAATGCC
  CTTAATGCCAGGGCAACACACACAGGAAATCAAATACCAGCATTTACACGTCAGTAACCC
  TTCAAGTTCTGCCACCCTGTGTGGGGTAATGCCGTGCAGCTAAAATATGATTTACGCAAC
  ACCATGACTAAGGAATTTCTCATAGAACTTAAATTTCTTTTGAAAGCTATTTTTTGTTTT
  TGGCAATAAGTCTATCCGGGCTTACTAAATAGTTGGCCCAATGTGCTTTGTGTGTGTTTT
  TAGAAACTTCTTCATTGTACCCATTACAGAAAAGTTCCATGTTATTGGTATTGAAAAACC
  AGAGGTGTCGTCCCTCTTACCCAGGGAGTTGCAAAGTGCTCTGGGAACACTATTTTCAGT
  GCTTCCTACCTACATGTCACTGACCGACCCATAGACCTCAGCCAGGGCCTGTGGCTAGGG
  GCCATCCCTGGAAGCCTTGGGGGCCATCTGCCTGCCGGCTGAATGGGGGCTGCAGGGCTG
  GTCTTGGACAGCTGCTCTCCCGTTGTGCAGCCTTCTTCTAAGTCCACCTCCAGGGGCTCC
  AAGGTCTGAACTTGAAAGN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001430
• Insert Size: 5500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001430.3, NP_001421.2
• RefSeq Size: 5186 bp
• RefSeq ORF: 2613 bp
• Locus ID: 2034
• Cytogenetics: 2p21
• Domains: PAS, HLH, PAC
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma
• Gene Summary: 'This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]'