HIF 2 alpha (EPAS1) (NM_001430) Human Untagged Clone

CAT#: SC320566

EPAS1 (untagged)-Human endothelial PAS domain protein 1 (EPAS1)


  "NM_001430" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol EPAS1
Synonyms bHLHe73; ECYT4; HIF2A; HLF; MOP2; PASD2
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_001430.3 CTTTTCCAGGGAAAAAGGAACTTGGGTTCCCTTCTCTCCGTCCTCTTTTCGGGTCTGACA
GCCTCCACCCACTCCTTCCCCGGACCCCGCCTCCGCGCGCAGGTTCCTCCCAGTCACCTT
TCTCCACCCCCGCCCCCGCACCTAGCCCGCCGCGCGCCACCTTCCACCTGACTGCGCGGG
GCGCTCGGGACCTGCGCGCACCTCGGACCTTCACCACCCGCCCGGGCCGCGGGGAGCGGA
CGAGGGCCACAGCCCCCCACCCGCCAGGGAGCCCAGGTGCTCGGCGTCTGAACGTCTCAA
AGGGCCACAGCGACAATGACAGCTGACAAGGAGAAGAAAAGGAGTAGCTCGGAGAGGAGG
AAGGAGAAGTCCCGGGATGCTGCGCGGTGCCGGCGGAGCAAGGAGACGGAGGTGTTCTAT
GAGCTGGCCCATGAGCTGCCTCTGCCCCACAGTGTGAGCTCCCATCTGGACAAGGCCTCC
ATCATGCGACTGGCAATCAGCTTCCTGCGAACACACAAGCTCCTCTCCTCAGTTTGCTCT
GAAAACGAGTCCGAAGCCGAAGCTGACCAGCAGATGGACAACTTGTACCTGAAAGCCTTG
GAGGGTTTCATTGCCGTGGTGACCCAAGATGGCGACATGATCTTTCTGTCAGAAAACATC
AGCAAGTTCATGGGACTTACACAGGTGGAGCTAACAGGACATAGTATCTTTGACTTCACT
CATCCCTGCGACCATGAGGAGATTCGTGAGAACCTGAGTCTCAAAAATGGCTCTGGTTTT
GGGAAAAAAAGCAAAGACATGTCCACAGAGCGGGACTTCTTCATGAGGATGAAGTGCACG
GTCACCAACAGAGGCCGTACTGTCAACCTCAAGTCAGCCACCTGGAAGGTCTTGCACTGC
ACGGGCCAGGTGAAAGTCTACAACAACTGCCCTCCTCACAATAGTCTGTGTGGCTACAAG
GAGCCCCTGCTGTCCTGCCTCATCATCATGTGTGAACCAATCCAGCACCCATCCCACATG
GACATCCCCCTGGATAGCAAGACCTTCCTGAGCCGCCACAGCATGGACATGAAGTTCACC
TACTGTGATGACAGAATCACAGAACTGATTGGTTACCACCCTGAGGAGCTGCTTGGCCGC
TCAGCCTATGAATTCTACCATGCGCTAGACTCCGAGAACATGACCAAGAGTCACCAGAAC
TTGTGCACCAAGGGTCAGGTAGTAAGTGGCCAGTACCGGATGCTCGCAAAGCATGGGGGC
TACGTGTGGCTGGAGACCCAGGGGACGGTCATCTACAACCCTCGCAACCTGCAGCCCCAG
TGCATCATGTGTGTCAACTACGTCCTGAGTGAGATTGAGAAGAATGACGTGGTGTTCTCC
ATGGACCAGACTGAATCCCTGTTCAAGCCCCACCTGATGGCCATGAACAGCATCTTTGAT
AGCAGTGGCAAGGGGGCTGTGTCTGAGAAGAGTAACTTCCTATTCACCAAGCTAAAGGAG
GAGCCCGAGGAGCTGGCCCAGCTGGCTCCCACCCCAGGAGACGCCATCATCTCTCTGGAT
TTCGGGAATCAGAACTTCGAGGAGTCCTCAGCCTATGGCAAGGCCATCCTGCCCCCGAGC
CAGCCATGGGCCACGGAGTTGAGGAGCCACAGCACCCAGAGCGAGGCTGGGAGCCTGCCT
GCCTTCACCGTGCCCCAGGCAGCTGCCCCGGGCAGCACCACCCCCAGTGCCACCAGCAGC
AGCAGCAGCTGCTCCACGCCCAATAGCCCTGAAGACTATTACACATCTTTGGATAACGAC
CTGAAGATTGAAGTGATTGAGAAGCTCTTCGCCATGGACACAGAGGCCAAGGACCAATGC
AGTACCCAGACGGATTTCAATGAGCTGGACTTGGAGACACTGGCACCCTATATCCCCATG
GACGGGGAAGACTTCCAGCTAAGCCCCATCTGCCCCGAGGAGCGGCTCTTGGCGGAGAAC
CCACAGTCCACCCCCCAGCACTGCTTCAGTGCCATGACAAACATCTTCCAGCCACTGGCC
CCTGTAGCCCCGCACAGTCCCTTCCTCCTGGACAAGTTTCAGCAGCAGCTGGAGAGCAAG
AAGACAGAGCCCGAGCACCGGCCCATGTCCTCCATCTTCTTTGATGCCGGAAGCAAAGCA
TCCCTGCCACCGTGCTGTGGCCAGGCCAGCACCCCTCTCTCTTCCATGGGGGGCAGATCC
AATACCCAGTGGCCCCCAGATCCACCATTACATTTTGGGCCCACAAAGTGGGCCGTCGGG
GATCAGCGCACAGAGTTCTTGGGAGCAGCGCCGTTGGGGCCCCCTGTCTCTCCACCCCAT
GTCTCCACCTTCAAGACAAGGTCTGCAAAGGGTTTTGGGGCTCGAGGCCCAGACGTGCTG
AGTCCGGCCATGGTAGCCCTCTCCAACAAGCTGAAGCTGAAGCGACAGCTGGAGTATGAA
GAGCAAGCCTTCCAGGACCTGAGCGGGGGGGACCCACCTGGTGGCAGCACCTCACATTTG
ATGTGGAAACGGATGAAGAACCTCAGGGGTGGGAGCTGCCCTTTGATGCCGGACAAGCCA
CTGAGCGCAAATGTACCCAATGATAAGTTCACCCAAAACCCCATGAGGGGCCTGGGCCAT
CCCCTGAGACATCTGCCGCTGCCACAGCCTCCATCTGCCATCAGTCCCGGGGAGAACAGC
AAGAGCAGGTTCCCCCCACAGTGCTACGCCACCCAGTACCAGGACTACAGCCTGTCGTCA
GCCCACAAGGTGTCAGGCATGGCAAGCCGGCTGCTCGGGCCCTCATTTGAGTCCTACCTG
CTGCCCGAACTGACCAGATATGACTGTGAGGTGAACGTGCCCGTGCTGGGAAGCTCCACG
CTCCTGCAAGGAGGGGACCTCCTCAGAGCCCTGGACCAGGCCACCTGAGCCAGGCCTTCT
ACCTGGGCAGCACCTCTGCCGACGCCGTCCCACCAGCTTCACTCTCTCCGTCTGTTTTTG
CAACTAGGTATTTCTAACGCCAGCACACTATTTACAAGATGGACTTACCTGGCAGACTTG
CCCAGGTCACCAAGCAGTGGCCTTTTTCTGAGATGCTCACTTTATTATCCCTATTTTTAA
AGTACACAATTGTTTTACCTGTTCTGAAATGTTCTTAAATTTTGTAGGATTTTTTTCCTC
CCCACCTTCAATGACTTCTAATTTATATTATCCATAGGTTTCTCTCCCTCCTTCTCCTTC
TCACACACAACTGTCCATACTAACAAGTTTGGTGCATGTCTGTTCTTCTGTAGGGAGAAG
CTTTAGCTTCATTTTACTAAAAAGATTCCTCGTTATTGTTGTTGCCAAAGAGAAACAAAA
ATGATTTTGCTTTCCAAGCTTGGTTTGTGGCGTCTCCCTCGCAGAGCCCTTCTCGTTTCT
TTTTTAAACTAATCACCATATTGTAAATTTCAGGGTTTTTTTTTTTGTTTAAGCTGACTC
TTTGCTCTAATTTTGGAAAAAAAGAAATGTGAAGGGTCAACTCCAACGTATGTGGTTATC
TGTGAAAGTTGCACAGCGTGGCTTTTCCTAAACTGGTGTTTTTCCCCCGCATTTGGTGGA
TTTTTTATTATTATTCAAAAACATAACTGAGTTTTTTAAAAGAGGAGAAAATTTATATCT
GGGTTAAGTGTTTATCATATATATGGGTACTTTGTAATATCTAAAAACTTAGAAACGGAA
ATGGAATCCTGCTCACAAAATCACTTTAAGATCTTTTCGAAGCTGTTAATTTTTCTTAGT
GTTGTGGACACTGCAGACTTGTCCAGTGCTCCCACGGCCTGTACGGACACTGTGGAAGGC
CTCCCTCTGTCGGCTTTTTGCCATCTGTGATATGCCATAGGTGTGACAATCCGAGCAGTG
GAGTCATTCAGCGGGAGCACTGCGCGCTATCCCCTCACATTCTCTATGTACTATGTATGT
ATGTATTATTATTATTGCTGCCAAGAGGGTCTGATGGCACGTTGTGGGGTCGGGGGGTGG
GGCGGGGAAGTGCTCTAACTTTTCTTAAGGTTTTGTTGCTAGCCCTTCAAGTGCACTGAG
CTATGTGACTCGGATGGTCTTTCACACGGCACATTTGGACATTTCCAGAACTACCATGAG
ATGGTTTAGACGGGAATTCATGCAAATGAGGGGTCAAAAATGGTATAGTGACCCCGTCCA
CGTCCTCCAAGCTCACGACCTTGGAGCCCCGTGGAGCTGGACTGAGGAGGAGGCTGCACA
GCGGGAGAGCAGCTGGTCCAGACCAGCCCTGCAGCCCCCACTCAGCCGGCAGCCAGATGG
CCCCGCAAGGCCTCCAGGGATGGCCCCTAGCCACAGGCCCTGGCTGAGGTCTCTGGGTCG
GTCAGTGACATGTAGGTAGGAAGCACTGAAAATAGTGTTCCCAGAGCACTTTGCAACTCC
CTGGGTAAGAGGGACGACACCTCTGGTTTTTCAATACCAATTACATGGAACTTTTCTGTA
ATGGGTACAATGAAGAAGTTTCTAAAAACACACACAAAGCACATTGGGCCAACTATTTAG
TAAGCCCGGATAGACTTATTGCCAAAAACAAAAAATAGCTTTCAAAAGAAATTTAAGTTC
TATGAGAAATTCCTTAGTCATGGTGTTGCGTAAATCATATTTTAGCTGCACGGCATTACC
CCACACAGGGTGGCAGAACTTGAAGGGTTACTGACGTGTAAATGCTGGTATTTGATTTCC
TGTGTGTGTTGCCCTGGCATTAAGGGCATTTTACCCTTGCAGTTTTACTAAAACACTGAA
AAATATTCCAAGCTTCATATTAACCCTACCTGTCAACGTAACGATTTCATGAACGTTATT
ATATTGTCGAATTCCTACTGACAACATTATAACTGTATGGGAGCTTAACTTTATAAGGAA
ATGTATTTTGACACTGGTATCTTATTAAAGTATTCTGATCCTAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_001430
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001430.3, NP_001421.2
RefSeq Size 5186 bp
RefSeq ORF 2613 bp
Locus ID 2034
Cytogenetics 2p21
Domains PAS, HLH, PAC
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Pathways in cancer, Renal cell carcinoma
Gene Summary 'This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]'

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