SLP76 (LCP2) (NM_005565) Human Untagged Clone

CAT#: SC110940

LCP2 (untagged)-Human lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa) (LCP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LCP2
• Synonyms: SLP-76; SLP76
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110940 sequence for NM_005565 edited (data generated by NextGen Sequencing)
  ATGGCACTGAGGAATGTGCCCTTTCGCTCAGAGGTCCTGGGCTGGGACCCCGACAGCCTT
  GCTGACTATTTCAAGAAGCTCAACTATAAGGACTGTGAGAAGGCAGTGAAGAAGTACCAC
  ATCGATGGGGCTCGCTTCTTGAACCTGACAGAAAATGACATCCAGAAGTTCCCCAAGCTC
  CGGGTGCCGATTCTCAGTAAGTTAAGTCAGGAAATCAACAAGAACGAAGAGAGGAGGAGC
  ATCTTCACACGCAAACCCCAAGTCCCGCGGTTTCCTGAAGAGACAGAAAGCCACGAAGAG
  GACAATGGGGGCTGGTCGTCCTTTGAAGAAGACGATTATGAAAGTCCCAATGATGACCAG
  GATGGGGAGGATGATGGAGACTATGAGTCCCCCAATGAGGAGGAAGAGGCACCCGTGGAA
  GATGACGCGGATTATGAGCCGCCACCCTCCAATGACGAGGAAGCTCTGCAGAACTCCATC
  CTGCCTGCCAAGCCTTTCCCCAACTCCAACTCCATGTACATCGACCGGCCCCCCTCTGGG
  AAAACCCCCCAGCAGCCTCCTGTGCCCCCCCAGAGACCGATGGCCGCCCTCCCGCCCCCA
  CCAGCCGGCCGGAATCACTCGCCACTGCCCCCACCCCAGACCAACCACGAAGAACCCAGC
  AGAAGCAGAAACCACAAAACGGCAAAGCTCCCTGCTCCTTCAATAGACAGAAGCACGAAA
  CCTCCTCTAGATCGTTCATTAGCTCCGTTTGATAGAGAACCCTTCACACTAGGAAAGAAA
  CCACCATTTTCTGACAAGCCCTCGATTCCAGCGGGAAGGTCACTCGGGGAGCATTTACCC
  AAGATTCAAAAGCCTCCTTTACCACCGACCACGGAAAGACATGAAAGGAGCAGCCCCCTG
  CCAGGGAAGAAGCCACCTGTGCCAAAGCATGGATGGGGACCAGACAGAAGAGAGAATGAT
  GAAGATGATGTGCATCAGAGACCTTTGCCCCAGCCAGCACTACTTCCTATGAGCTCCAAC
  ACTTTCCCTTCAAGATCTACTAAGCCAAGTCCCATGAACCCTCTCCCATCCTCTCACATG
  CCTGGAGCATTCTCAGAAAGTAACAGCAGTTTTCCACAGAGTGCCTCCCTGCCACCATAC
  TTCTCTCAAGGCCCTAGCAACAGACCACCTATCAGAGCCGAAGGCAGAAACTTCCCCTTG
  CCACTTCCAAACAAACCTCGGCCCCCATCCCCCGCGGAGGAAGAGAATTCATTAAATGAA
  GAGTGGTACGTTTCTTATATTACCCGACCAGAGGCAGAAGCTGCTCTTAGAAAGATAAAC
  CAGGATGGCACATTTCTGGTCAGAGACAGCTCTAAAAAAACAACAACCAATCCATATGTC
  CTCATGGTGTTGTACAAAGATAAAGTTTACAACATCCAGATCCGTTATCAGAAGGAAAGT
  CAAGTTTACTTGTTGGGAACTGGACTCCGAGGGAAAGAGGACTTTCTGTCTGTGTCAGAT
  ATTATTGACTACTTCAGGAAAATGCCACTTCTGCTCATTGATGGGAAAAACCGAGGTTCC
  AGATACCAGTGCACATTAACGCATGCTGCAGGGTACCCATAG
  
  Clone variation with respect to NM_005565.3
  726 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005565 unedited
  GGGTGCACATTAGGNATACGATTCCTATAGGCGGCCCGCGAATTCGCACGAGAAAACTCG
  GCTCCAACAGGGGTGTTCCACAGGGTAGCTGGGAGTTGGAAGAGCCAAGAACGCCTCCGA
  GCTCTGGATTTGAGCTTCTCTGCCCATGGGTGAAGCGCCCATGCTCAGCTTGTGAGCTTC
  TTCCCGGGAGAGCAGCCATGGCACTGAGGAATGTGCCCTTTCGCTCAGAGGTCCTGGGCT
  GGGACCCCGACAGCCTTGCTGACTATTTCAAGAAGCTCAACTATAAGGACTGTGAGAAGG
  CAGTGAAGAAGTACCACATCGATGGGGCTCGCTTCTTGAACCTGACAGAAAATGACATCC
  AGAAGTTCCCCAAGCTCCGGGTGCCGATTCTCAGTAAGTTAAGTCAGGAAATCAACAAGA
  ACGAAGAGAGGAGGAGCATCTTCACACGCAAACCCCAAGTCCCGCGGTTTCCTGAAGAGA
  CAGAAAGCCACGAAGAGGACAATGGGGGCTGGTCGTCCTTTGAAGAAGACGATTATGAAA
  GTCCCAATGATGACCAGGATGGGGAGGATGATGGAGACTATGAGTCCCCCAATGAGGAGG
  AAGAGGCACCCGTGGAAGATGACGCGGATTATGACCCGCCACCCTCCAATGACCAGGAAG
  CTCTGCAGAACTCCATCCTGCCTGCCAAGCCTTTCCCCAACTTCAACTCCATGTCCATTC
  GACCGGCCCCCCTCTTGGGAAAACCCCCCAAAAGCCTCTCTGCGCCCCACCCACAGACCC
  GAGGGCCCGCCCTCCCCCCCCCCACCATCCGGCCGGAAAACACTCGGCCCTTGCCTCCAC
  TCCCATCCACCCACGAACAATCCCCCTCCAACATCAACCCCCCAACGCGTCAGCCCCCCT
  CGCCCTTCCACTCCTCACAAATCCCCCCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005565 unedited
  GCGGCCGCTTTCTAGTATCGAGTTTTTTTTTTTTTTTTTTAAAGCAAAAAACTATTTTAA
  TCCCAGAAGTTATCTCAACATGCATTTGTCTTTTCATAAAAAAATTACGTAAAAATCCCC
  CCAGGAAAAAATACTAAATAAGAGTGAAATTATATTTTTGAAGCCTCTTAAATTATTGCT
  GCTGAGAAAACGAGGTTTAGTGATTTTCAAATTACATGTATAAAACTAATGAATGCCTCT
  GACTGACAGAGGTTTGGCTGTAAAAAACAAATCAGTTTTATGTGACACCAATAACTATTA
  CTGTTTCAGTGTTTTAAAACTTGCCCCAAATTGTTTCTGTAGCTGTGTAAACTACTGTAT
  TTTGAAATGGGCATTAAATAATCTTTTAAAAAATGAATTATTACAGTGCACTACTAGACT
  TCAAGTGTGAACATGACTCATGCACTTTACAAACATTGAAGAAGATTAAATAAATTATGG
  GATAGTCGACAGTCTTCATTTCAAACACTGTTTTTAAAGGAAAGGATAAAACCCTTGTGT
  TCATGGGGAGGGGTTCAGTTCATTCCTAAGTGTTTGTCCATTGACCAAAGCTGATGGATC
  TCGTGTCGGCAACAGAGGCAGGAGGACGGTTCATTTGCTCGGCTAAACTTGCTATGGGTA
  CCCTGCATCACGCGTCATTGTGCCCTGGTATTCGGGAACCTCGGGTGTTCCCCTTATAGA
  TCAAAAGTGGCCTTTTCCTGAATCTGGCATTATTTCTGACCCAGACAAAAACTCTTCTTC
  TTTGTATCCACTCGCAATATCTAACCCTGTCTTCCTCTTAAACCGATCTTTATGCTTATT
  CCTTATTTTCTCCCTCCCGTAGGCATTGTTCCGTCGCCCTCTCTTACACTCTCCTCCCTA
  TATCGCCTCTCTCGACCCTTCTAATATCCACTTCTCTCGACGCCTCTTATGTACATTCCT
  TTCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005565
• Insert Size: 2390 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005565.3, NP_005556.1
• RefSeq Size: 2472 bp
• RefSeq ORF: 1602 bp
• Locus ID: 3937
• Cytogenetics: 5q35.1
• Domains: SH2, SAM
• Protein Pathways: Fc epsilon RI signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway
• Gene Summary: 'This gene encodes an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. The encoded protein associates with growth factor receptor bound protein 2, and is thought to play a role TCR-mediated intracellular signal transduction. A similar protein in mouse plays a role in normal T-cell development and activation. Mice lacking this gene show subcutaneous and intraperitoneal fetal hemorrhaging, dysfunctional platelets and impaired viability. [provided by RefSeq, Nov 2016]'