Superoxide Dismutase 1 (SOD1) (NM_000454) Human Untagged Clone

CAT#: SC111022

SOD1 (untagged)-Human superoxide dismutase 1, soluble (SOD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SOD1
• Synonyms: ALS; ALS1; HEL-S-44; homodimer; hSod1; IPOA; SOD; STAHP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111022 sequence for NM_000454 edited (data generated by NextGen Sequencing)
  ATGGCGACGAAGGCCGTGTGCGTGCTGAAGGGCGACGGCCCAGTGCAGGGCATCATCAAT
  TTCGAGCAGAAGGAAAGTAATGGACCAGTGAAGGTGTGGGGAAGCATTAAAGGACTGACT
  GAAGGCCTGCATGGATTCCATGTTCATGAGTTTGGAGATAATACAGCAGGCTGTACCAGT
  GCAGGTCCTCACTTTAATCCTCTATCCAGAAAACACGGTGGGCCAAAGGATGAAGAGAGG
  CATGTTGGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTCTATT
  GAAGATTCTGTGATCTCACTCTCAGGAGACCATTGCATCATTGGCCGCACACTGGTGGTC
  CATGAAAAAGCAGATGACTTGGGCAAAGGTGGAAATGAAGAAAGTACAAAGACAGGAAAC
  GCTGGAAGTCGTTTGGCTTGTGGTGTAATTGGGATCGCCCAATAA
  
  Clone variation with respect to NM_000454.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000454 unedited
  ACCGGTTTTGAAACCGACTCATATAGGCGGCAACCAATTCGCACGAGGTGCTGGTTTGCG
  TCGTAGTCTCCTGCAGCGTCTGGGGTTTCCGTTGCAGTCCTCGGAACCAGGACCTCGGCG
  TGGCCTATCGAGTTATGGCGACGAAGGCCGTGTGCGTGCTGAAGGGCGACGGCCCAGTGC
  AGGGCATCATCAATTTCGAGCAGAATGTTTTTTATGGACCAGTGAAGGTGTGGGGAAGCA
  TTAAAGGACTGACTGAAGGCCTGCATGGATTCCATGTTCATGAGTTTGGAGATAATACAG
  CAGGCTGTACCAGTGCAGGTCCTCACTTTAATCCTCTATCCAGAAAACACGGTGGGCCAA
  AGGATGAAGAGAGGCATGTTGGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGG
  CCGATGTGTCTATTGAAGATTCTGTGATCTCACTCTCAGGAGACCATTGCATCATTGGCC
  GCACACTGGTGGTCCATGAAAAAGCAGATGACTTGGGCAAAGGTGGAAATGAAGAAAGTA
  CAAAGACAGGAAACGCTGGAAGTCGTTTGGCTTGTGGTGTAATTGGGATCGCCCAATAAA
  CATTCCCTTGGATGTAGTCTGAGGCCCCTTAACTCATCTGTTATCCTGCTAGCTGTAGAA
  ATGTATCCTGATAAACATTAAACACTGTAATCTTAAAAGTGTAATTGTGTGACTTTTTCA
  GAGTTGCTTTAAAGTACCTGTAGTGAGAAACTGATTTATGATCACTTGGAAGATCTGTAT
  AGNTTTATAAAACTCAGTTAAAATGTCTGTTTCAATGACCTGTATTTTGCCACGACTTAA
  TCACAGATGGGTATTAAACCTGTCAGAATTTCTTGTCATTCAAGCCTTGAATAAAAAACC
  TGTATGGCACTTTTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000454 unedited
  GTCTGCCCGCGGCCGCATCTAGGTCGGCTTTTTTTTTTTTTTTTTGAATTTGGATTCTTT
  TAATAGCCTCATAATAAGTGCCATACAGGGTTTTTATTCACAGGCTTGAATGACAAAGAA
  ATTCTGACAAGTTTAATACCCATCTGTGATTTAAGTCTGGCAAAATACAGGTCATTGAAA
  CAGACATTTTAACTGAGTTTTATAAAACTATACAAATCTTCCAAGTGATCATAAATCAGT
  TTCTCACTACAGGTACTTTAAAGCAACTCTGAAAAAGTCACACAATTACACTTTTAAGAT
  TACAGTGTTTAATGTTTATCAGGATACATTTCTACAGCTAGCAGGATAACAGATGAGTTA
  AGGGGCCTCAGACTACCTCCACGGGAATGTTTATCGGGCGATCCCAATTACACCACAAGC
  CAAACGACTTCCAGCGTTTCCTGTCTTTGCACTTTCTTCATTTTCACCTTTGCCCAAGTC
  ATCTGCTTTTTCATGGACCACCAGTGTGCGGCCAATGATGCAATGGTCTCCTGAGAGTGA
  GATCACAGAATCTTCACTAGACACATCGGCCACACCATCTTTGTCAGCAGCCACATTGCC
  CAAGTCTCCAACATGCCTCTNTTTATCCTTTGGCCCACCGTGTTTTCTGGATAGAGGATC
  AAAGTGATGACCCTGTCCTGTCACCGCCTGCTGTATTATCTCCAAACTCATGAACATGGC
  ATCCACTGCGGCCCTAACTCACCTCTTTAATGCTTCCCCAACCCTTACTGGCCATTCACT
  TCCTTTTTGCTCGACAATGATGAATGCCCTGCCCTGAGCCCGTCGTCCTTTTTCCCCCCC
  AACGCACTTTGTTCCCCTCACCTCCGTTTAGCCCCGCCCCAGTTCCTGGCTTCCAGGGAC
  TGCAACCGAAACCCCACAACCCTGGAGGAGAATACGAGCCAAACCATATACCGCCGCGCG
  CATTCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000454
• Insert Size: 940 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000454.4, NP_000445.1
• RefSeq Size: 981 bp
• RefSeq ORF: 465 bp
• Locus ID: 6647
• Cytogenetics: 21q22.11
• Domains: sodcu
• Protein Families: Druggable Genome
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), Huntington's disease, Prion diseases
• Gene Summary: 'The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. In addition, this protein contains an antimicrobial peptide that displays antibacterial, antifungal, and anti-MRSA activity against E. coli, E. faecalis, S. aureus, S. aureus MRSA LPV+, S. agalactiae, and yeast C. krusei. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2020]'