SURF1 (NM_003172) Human Untagged Clone

CAT#: SC111028

SURF1 (untagged)-Human surfeit 1 (SURF1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SURF1
• Synonyms: CMT4K
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111028 sequence for NM_003172 edited (data generated by NextGen Sequencing)
  ATGGCGGCGGTGGCTGCGTTGCAGCTGGGGCTGCGGGCGGCGGGGCTGGGACGGGCCCCG
  GCCAGCGCCGCCTGGAGGAGCGTCCTCAGGGTCTCCCCGCGCCCAGGGGTGGCCTGGAGG
  CCAAGCAGATGTGGCAGTTCTGCAGCAGAAGCATCTGCCACAAAAGCGGAAGATGACTCC
  TTTCTTCAGTGGGTCCTGCTCCTCATCCCTGTGACTGCCTTTGGCTTGGGGACATGGCAG
  GTCCAGCGTCGGAAGTGGAAGCTGAACCTGATTGCAGAGTTGGAGTCCAGAGTTCTGGCT
  GAGCCTGTCCCTCTGCCAGCCGACCCAATGGAACTGAAAAATCTGGAGTATAGGCCAGTG
  AAGGTCAGGGGGTGCTTTGACCATTCCAAGGAGCTGTATATGATGCCCCGGACCATGGTG
  GACCCTGTCCGGGAGGCCCGGGAGGGCGGCCTCATCTCCTCCTCAACTCAGAGTGGGGCC
  TATGTGGTCACTCCCTTCCACTGCACCGACCTGGGAGTCACCATCCTGGTAAATAGAGGG
  TTCGTTCCCAGGAAGAAAGTGAATCCTGAAACCCGGCAGAAAGGCCAGATTGAGGGAGAA
  GTGGACCTCATTGGGATGGTGAGGCTGACAGAAACCAGGCAGCCTTTTGTCCCTGAGAAC
  AATCCAGAAAGGAACCACTGGCATTATCGAGACCTGGAAGCTATGGCCAGAATCACAGGC
  GCAGAGCCCATCTTCATTGATGCCAACTTCCAGAGCACAGTCCCTGGAGGACCCATTGGA
  GGGCAAACCAGAGTTACTCTGAGGAACGAGCATCTGCAGTACATCGTGACCTGGTATGGA
  CTCTCTGCAGCTACATCCTACCTGTGGTTTAAGAAATTCCTACGTGGGACACCTGGTGTG
  TGA
  
  Clone variation with respect to NM_003172.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003172 unedited
  GGTTCACCAATTTGTAATCCGCACTCACTATAGGGNNCGGCCGCGCAATTCGGCACGAGG
  GAAGCGCCCGCGGGGCCGGGTGCGATGGCGGCGGTGGCTGCGTTGCAGCTGGGGCTGCGG
  GCGGCGGGGCTGGGACGGGCCCCGGCCAGCGCCGCCTGGAGGAGCGTCCTCAGGGTCTCC
  CCGCGCCCAGGGGTGGCCTGGAGGCCAAGCAGATGTGGTAGTTCTGCAGCAGAAGCATCT
  GCCACAAAAGCGGAAGATGACTCCTTTCTTCAGTGGGTCCTGCTCCTCATCCCTGTGACT
  GCCTTTGGCTTGGGGACATGGCAGGTCCAGCGTCGGAAGTGGAAGCTGAACCTGATTGCA
  GAGTTGGAGTCCAGAGTTCTGGCTGAGCCTGTCCCTCTGCCAGCCGACCCAATGGAACTG
  AAAAATCTGGAGTATAGGCCAGTGAAGGTCAGGGGGTGCTTTGACCATTCCAAGGAGCTG
  TATATGATGCCCCGGACCATGGTGGACCCTGTCCCGGGAGGCCCGGGAGGGCGGCCTCAT
  CTCCTCCTCAACTCAGAGTGGGGCCTATGTGGTCACTCCCTTCCACTGCACCGACCTGCG
  AGTCACCATCCCTGGTAAATAGAGGGTTCGTTCCCAGGAAGAAAGTGAATCCTGAAACCC
  GGCAGAAAAGCCCGATTGAAGGAAAAGTGGACCTCATTGCGATGGTGAGGCTGACAGAAA
  CCACGCAGCCTTTTGCCCCTGATACAATCCAGAAAGGAACCACTGGCTTATCGAGACCTG
  GAAGCTTTGGCACAATCCCAGGCGCAGAAGCCATTTTCATTAATGCCACCTCCCAAGACC
  CGTCCCTGGAGGACCCATTGGAGGCCAAACCATGATTTCTTCTGAGGACACAGCATCTGC
  AGAACATTGCGACCTGGTATGGACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003172 unedited
  GGACCGCGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTAAGGGAAAAGGCCAAAACTTTATACCAGTAGCACATGATCCAGCATAAAGGCA
  GTCTTGAAATACTGCATTATCCAGGGACAGGGCTTCAGCAGCTGATCTGTCACACACCAG
  GTGTCCCACGTAGGAATTTCTTAAACCACAGGTAGGATGTAGCTGCAAAAAGTCCATACC
  AGGTCACGATGTACTGCAAATGCTCGTTCCTCAAAGTAACTCTGGTTTGCCCTCCAATGG
  GTCCTCCAGGGACTGTGCTCTGGAAGTTGGCATCAATGAAAATGGGCTCTGCGCCTGTGA
  TTCTGGCCATAGCTTCCAGGTCTCGATAATGCCAGGGGTTCCTTTCTGGATTGTTCTCAG
  GGACAAAAGGCTGCCTGGTTTCTGTCAGCCTCACCATCCCAATGAGGTCCACTTCTCCCT
  CAATCTGGCCTTTCTGCCGGGTTTCAGGATTCACTTTCTTCCTGGGAACGAACCCTCTAT
  TTACCAGGATGGTGACTCCCAGGTCGGTGCAGTGGAAGGGAGTGACCACATAGGCCCCAC
  TCTGAGTTGAGGAGGAGATGAGGCCGCCCTCCCGGGCCTCCCGGACAGGGTCCACCATGG
  TCCGGGGCATCATATACAGCTCCTTGGAATGGTCAAAGCACCCCCTGACCTTCACTGGCC
  TATACTCCAGATTTTTCANTTCCATTGGGTCGGCTGGCAAAGGGACAGGCTCACCCAAAC
  TCTGGACTTCACTCTGCATCAGGGTCAGCTTCCACTTCCGACGCTGGACCTGCCATGTCC
  CCAAGCCAAGGCAGTCACAGGGATGAGGGAGCAGGACCACTGAAAAAGGGAGTCATCTCC
  GCTTTGNGGCAAATGCTCTGCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003172
• Insert Size: 1070 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003172.2, NP_003163.1
• RefSeq Size: 1037 bp
• RefSeq ORF: 903 bp
• Locus ID: 6834
• Cytogenetics: 9q34.2
• Domains: SURF1
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a protein localized to the inner mitochondrial membrane and thought to be involved in the biogenesis of the cytochrome c oxidase complex. The protein is a member of the SURF1 family, which includes the related yeast protein SHY1 and rickettsial protein RP733. The gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity, where it shares a bidirectional promoter with SURF2 on the opposite strand. Defects in this gene are a cause of Leigh syndrome, a severe neurological disorder that is commonly associated with systemic cytochrome c oxidase deficiency. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).