USP1 (NM_003368) Human Untagged Clone

CAT#: SC111053

USP1 (untagged)-Human ubiquitin specific peptidase 1 (USP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP1
• Synonyms: UBP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003368 edited
  CCTCGGTGGCGGAGTGCTAAAGACCCTAGCGGTTCAGGCGTTCGGCGAGCGGGGCCGCTG
  CTTGTTGCGCTCCTGGCTCTCCCGGGGCGGGCGCAGATGGGCGCCGCTCCCGGGATGTAG
  TTGGTGTTGGTGCAAGACGGGAGCGAGCGGCGGTCGGGGTTCCCGCTCTTGGGAGCGGAT
  GGTCACTCCCCCGCGGGGAGGGCGAGCCGACCAGATTTTCCTGGGGCCGGGGACCCGGCG
  GGCTCGGGGCAGGGACTCACCTGTCGCACCCACACTCATTCGGGTTGGACTTGCCGGCGT
  CACCGCCGCGGACTTCGCTTTGGGCCATGACCAGATATAATTGGTGATTACAACTTTCCT
  CTATAAATTAACTCTTGACACTCCTTGGGATTTGAAGAAAAAAATGCCTGGTGTCATACC
  TAGTGAAAGTAATGGACTTTCAAGAGGTAGCCCTTCAAAGAAAAACAGACTTTCCTTAAA
  GTTTTTTCAGAAAAAGGAAACTAAGAGAGCTTTGGATTTCACAGATTCTCAAGAAAATGA
  AGAAAAAGCTTCTGAATATAGAGCATCTGAAATTGATCAAGTTGTTCCTGCAGCACAGTC
  TTCACCTATAAACTGTGAGAAGAGAGAAAACTTGTTACCATTTGTGGGACTGAATAATCT
  CGGCAATACTTGCTATCTTAATAGTATACTTCAGGTATTATATTTTTGTCCCGGTTTTAA
  ATCTGGAGTAAAGCACTTATTTAATATTATTTCAAGGAAGAAAGAAGCTCTAAAGGATGA
  AGCCAATCAAAAAGACAAGGGAAATTGCAAAGAAGATTCTTTGGCAAGTTATGAATTGAT
  ATGCAGTTTACAGTCCTTAATCATTTCGGTTGAACAGCTCCAGGCTAGTTTTCTCTTAAA
  TCCAGAGAAATATACTGATGAACTTGCCACTCAGCCAAGGCGACTGCTTAACACACTGAG
  GGAACTCAACCCTATGTATGAAGGATATCTACAGCATGATGCACAGGAAGTATTACAATG
  TATTTTGGGAAACATTCAAGAAACATGCCAACTCCTAAAAAAA:GAAGAAGTAAAAAATG
  TGGCAGAATTACCTACTAAGGTAGAAGAAATACCTCATCCGAAAGAGGAAATGAATGGTA
  TTAACAGCATAGAGATGGACAGTATGAGGCATTCTGAAGACTTTAAAGAGAAACTCCCAA
  AAGGAAATGGGAAAAGAAAAAGTGACACTGAATTTGGTAACATGAAGAAAAAAGTTAAAT
  TATCCAAGGAACACCAGTCATTGGAAGAGAACCAGAGACAAACTAGATCAAAAAGAAAAG
  CTACAAGTGATACATTAGAGAGTCCTCCTAAAATAATTCCCAAGTATATTTCTGAAAATG
  AGAGTCCAAGACCCTCACAAAAGAAATCAAGAGTTAAAATAAATTGGTTAAAGTCTGCAA
  CTAAGCAACCCAGCATTCTTTCTAAATTTTGTAGTCTGGGAAAAATAACAACAAACCAAG
  GAGTCAAAGGACAATCTAAAGAAAATGAATGTGATCCTGAAGAGGACTTGGGGAAGTGTG
  AAAGTGATAACACAACTAATGGTTGTGGACTTGAATCTCCAGGAAATACTGTTACACCTG
  TAAATGTTAATGAAGTTAAACCCATAAACAAAGGTGAAGAACAAATTGGTTTTGAGCTAG
  TGGAGAAATTATTTCAAGGTCAGCTGGTATTAAGGACGCGTTGCTTGGAATGTGAAAGTT
  TAACAGAAAGAAGAGAAGATTTTCAAGACATCAGTGTGCCAGTACAAGAAGATGAGCTTT
  CCAAAGTAGAGGAGAGTTCTGAAATTTCTCCAGAGCCAAAAACAGAAATGAAGACCCTGA
  GATGGGCAATTTCACAATTTGCTTCAGTAGAAAGGATTGTAGGAGAAGATAAATATTTCT
  GTGAAAACTGCCATCATTATACTGAAGCTGAACGAAGTCTTTTGTTTGACAAAATGCCTG
  AAGTTATAACTATTCATTTGAAGTGCTTTGCTGCTAGTGGTTTGGAGTTTGATTGTTATG
  GTGGTGGACTTTCCAAGATCAACACTCCTTTATTGACACCTCTTAAATTGTCACTAGAAG
  AATGGAGCACAAAGCCAACTAACGACAGCTATGGATTATTTGCGGTTGTGATGCATAGTG
  GCATTACAATTAGTAGTGGGCATTACACTGCTTCTGTTAAAGTCACTGACCTTAACAGTT
  TAGAACTAGATAAAGGAAATTTTGTGGTTGACCAAATGTGTGAAATAGGTAAGCCAGAAC
  CATTGAATGAGGAGGAAGCAAGGGGTGTGGTTGAGAATTATAATGATGAAGAAGTGTCAA
  TTAGAGTTGGTGGAAATACACAGCCAAGTAAAGTTTTGAACAAAAAAAATGTAGAAGCTA
  TTGGACTTCTTGGAGGACAAAAGAGCAAAGCAGATTATGAGCTATACAACAAAGCCTCTA
  ATCCTGATAAGGTTGCTAGTACAGCGTTTGCTGAAAATAGAAATTCTGAGACTAGTGATA
  CTACTGGGACCCATGAATCTGATAGAAACAAGGAATCCAGTGACCAAACAGGCATTAATA
  TTAGTGGATTTGAGAACAAAATTTCATACGTAGTGCAAAGCTTAAAGGAGTATGAGGGGA
  AGTGGTTGCTTTTTGATGATTCTGAAGTCAAAGTTACTGAAGAGAAGGACTTTCTGAATT
  CTCTTTCCCCTTCTACATCTCCTACTTCTACTCCTTACTTGCTATTTTATAAGAAATTAT
  AGAGTGAGTGTATTTTCCTTGTGTATATATTAAACACACCCATACAAACATTGGTAAAGT
  TGATTACATCAAAGAATCTTTAGCTTATCTTTTGAAGCTACTGGATATTATTGGTCTCTC
  TAGGTTTTTATATAAATAGTGAAATTTGAATTACTGAAAACCATGTTAATTTTTAGAACT
  CATTTTCCTCAGTAGAGACTAGTGATGCATTAGCTTCTGGGAACAAACTTGTATCGGTTC
  TTAATTAAATTATCCAAAACGGAGGCATTTAAACACTTGGATTTACACCAGTCTTTTGTG
  TTTGCTTTTTAAAATAAAGTGCTCGTATTTGTATTCTCCATATTTTGGAGTAATTATCTA
  CATGATGTTTATAGTTCCTGTGGTTTTTCACCCAAGAAGCAGAATCTCATTCAGTACATT
  TAGTTTTATAAGAGTCATGAAGCTAAATCCTTGGGCTATGTCAGAGGCACAAAGTCTAGA
  ATGTGTGTATTCACAATGGTGTATGTACATTTTGTGCCTTGATTCACTTAGAAGTGTCTC
  AGAAAACCTGGACAGTTCGCTTCTACACAAGAATTTTATATGTATTTATGAAGATGATTC
  TGTACCCTAGTATATCTTTTTGGGCATGGACTAATTTGTATCTGTTTAACTCATATTCTG
  CACGATCTGTATATAGTACATCAAACTTAGAGGTGTGACCTTAAATTTAACTTTTTTTAA
  AAACTGGGAGGTCAATAAAATTTAAACTGCTTAACTATGAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_003368
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_003368.4.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003368.4, NP_003359.3
• RefSeq Size: 3949 bp
• RefSeq ORF: 2358 bp
• Locus ID: 7398
• Cytogenetics: 1p31.3
• Domains: UCH
• Protein Families: Protease
• Gene Summary: 'This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.