POLK (NM_016218) Human Untagged Clone

CAT#: SC111277

POLK (untagged)-Human polymerase (DNA directed) kappa (POLK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLK
• Synonyms: DINB1; DINP; POLQ
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111277 sequence for NM_016218 edited (data generated by NextGen Sequencing)
  ATGGATAGCACAAAGGAGAAGTGTGACAGTTACAAAGATGATCTTCTGCTTAGGATGGGA
  CTTAATGATAATAAAGCAGGAATGGAAGGATTAGATAAAGAGAAAATTAACAAAATTATA
  ATGGAAGCCACGAAGGGGTCCAGATTTTATGGAAATGAGCTCAAGAAAGAAAAGCAAGTC
  AACCAACGAATTGAAAATATGATGCAACAAAAAGCTCAAATCACCAGCCAACAGCTAAGA
  AAAGCACAATTACAGGTTGACAGATTTGCAATGGAATTAGAACAAAGCCGAAATTTGAGC
  AATACCATAGTGCACATTGACATGGATGCTTTCTATGCAGCTGTAGAAATGAGGGACAAT
  CCAGAATTGAAGGATAAACCCATTGCTGTAGGATCAATGAGTATGCTGTCTACTTCAAAT
  TACCATGCAAGGAGATTTGGTGTTCGTGCAGCCATGCCAGGATTTATTGCTAAGAGGCTG
  TGCCCACAACTTATAATAGTGCCCCCCAACTTTGACAAATACCGAGCTGTGAGTAAAGAG
  GTTAAGGAAATACTTGCTGATTATGATCCCAATTTTATGGCCATGAGTCTTGATGAAGCC
  TACTTGAATATAACAAAGCACTTAGAAGAAAGACAAAATTGGCCTGAGGATAAAAGAAGG
  TATTTCATCAAAATGGGAAGCTCTGTAGAAAATGATAATCCAGGAAAGGAAGTTAATAAA
  CTGAGTGAGCATGAACGATCCATCTCTCCACTACTTTTTGAAGAGAGTCCTTCTGATGTG
  CAGCCCCCAGGAGATCCTTTCCAAGTGAACTTTGAAGAACAAAACAATCCTCAAATACTC
  CAAAACTCAGTTGTTTTTGGAACATCAGCCCAGGAAGTGGTAAAGGAAATTCGTTTCAGA
  ATTGAGCAGAAAACAACACTGACAGCTAGTGCAGGCATTGCCCCAAATACAATGTTAGCA
  AAAGTATGCAGTGATAAGAATAAACCAAATGGACAATACCAAATTCTTCCCAATAGACAA
  GCTGTGATGGACTTCATCAAGGATTTACCCATTAGAAAGGTTTCTGGAATAGGAAAAGTT
  ACAGAGAAAATGTTAAAGGCCCTTGGAATTATTACATGTACAGAACTTTACCAACAGAGG
  GCATTGCTTTCTCTCCTTTTCTCTGAAACATCTTGGCATTATTTCCTTCATATCTCCTTG
  GGTCTAGGTTCAACACACCTGACGAGGGATGGAGAGAGGAAAAGTATGAGCGTTGAGAGG
  ACATTCAGTGAGATAAATAAAGCGGAAGAGCAATACAGCCTATGTCAAGAACTTTGCAGT
  GAGCTTGCTCAGGATCTACAGAAAGAAAGACTTAAGGGTAGAACTGTTACCATTAAGTTG
  AAGAATGTGAATTTTGAAGTAAAAACTCGTGCATCTACAGTTTCATCTGTTGTTTCTACT
  GCAGAAGAAATATTTGCCATTGCTAAGGAATTGCTAAAAACAGAAATTGATGCTGATTTT
  CCACATCCCTTGAGATTAAGGCTTATGGGTGTTCGGATATCTAGTTTTCCCAATGAAGAG
  GACAGGAAACACCAACAAAGGAGCATTATTGGCTTTTTACAGGCTGGAAACCAAGCCCTG
  TCAGCCACTGAGTGTACATTAGAGAAAACTGACAAAGATAAGTTTGTAAAACCTCTAGAA
  ATGTCTCATAAGAAGAGTTTCTTTGATAAAAAACGATCAGAAAGGAAATGGAGTCACCAA
  GATACATTTAAATGTGAAGCCGTGAATAAACAAAGTTTCCAGACATCACAACCATTCCAA
  GTTTTAAAGAAGAAGATGAATGAGAATTTGGAAATATCAGAGAATTCAGATGACTGTCAG
  ATACTTACCTGTCCTGTTTGCTTTAGGGCTCAAGGGTGCATCAGTCTGGAAGCCTTGAAT
  AAACATGTAGATGAATGTCTTGATGGACCTTCAATCAGTGAAAACTTTAAAATGTTCTCG
  TGTTCACATGTTTCTGCTACCAAAGTTAACAAGAAAGAAAATGTTCCTGCTTCTTCACTT
  TGTGAGAAGCAAGATTATGAAGCCCATCCAAAAATTAAAGAAATATCTTCAGTAGATTGT
  ATAGCTTTAGTAGATACTATAGATAACTCATCTAAAGCAGAAAGCATAGATGCTTTAAGT
  AATAAGCATAGCAAGGAAGAATGTTCTAGTCTCCCAAGCAAGTCTTTTAATATTGAACAC
  TGTCATCAGAATTCTTCTTCTACTGTTTCATTGGAAAACGAAGATGTTGGATCATTTAGA
  CAAGAATACCGCCAGCCTTACTTATGTGAAGTGAAAACAGGCCAAGCTCTAGTTTGTCCT
  GTTTGTAACGTAGAACAAAAGACTTCAGATCTAACCCTGTTCAATGTGCATGTGGATGTT
  TGCTTAAATAAAAGTTTTATCCAAGAATTAAGAAAGGATAAATTTAACCCAGTTAATCAA
  CCCAAAGAAAGCTCCAGAAGTACTGGTAGCTCAAGTGGAGTACAGAAGGCTGTAACAAGA
  ACAAAAAGGCCAGGATTGATGACAAAGTACTCAACATCAAAGAAAATAAAACCAAACAAT
  CCCAAACATACCCTTGATATATTTTTTAAGTAA
  
  Clone variation with respect to NM_016218.2
  927 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016218 unedited
  TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAACGGATAAGTTTATACC
  ATGGATAGCACAAAGGAGAAGTGTGACAGTTACAAAGATGATCTTCTGCTTAGGATGGGA
  CTTAATGATAATAAAGCAGGAATGGAAGGATTAGATAAAGAGAAAATTAACAAAATTATA
  ATGGAAGCCACGAAGGGGTCCAGATTTTATGGAAATGAGCTCAAGAAAGAAAAGCAAGTC
  AACCAACGAATTGAAAATATGATGCAACAAAAAGCTCAAATCACCAGCCAACAGCTAAGA
  AAAGCACAATTACAGGTTGACAGATTTGCAATGGAATTAGAACAAAGCCGAAATTTGAGC
  AATACCATAGTGCACATTGACATGGATGCTTTCTATGCAGCTGTAGAAATGAGGGACAAT
  CCAGAATTGAAGGATAAACCCATTGCTGTAGGATCAATGAGTATGCTGTCTACTTCAAAT
  TACCATGCAAGGAGATTTGGTGTTCGTGCAGCCATGCCAGGATTTATTGCTAAGAGGCTG
  TGCCCACAACTTATAATAGTGCCCCCCAACTTTGACAAATACCGAGCTGTGAGTAAAGAG
  GTTAAGGAAATACTTGCTGATTATGATCCCAATTTTATGGCCATGAGTCTTGATGAAGCC
  TACTTGAATATAACAAAGCACTTAGAAGAAAGAACAAATTGGCCTGAGGATAAAAGAAGG
  TATTTCATCAAAATGGGAAGCTCTGTAGAAAATGATAATCCAGGAAAGGAAGTAATANAC
  TGAGTGAGCATGAACGATCCATCTCTCCACTACTTTTTGAAGAGAGTCCTTCTGATGTGC
  AGCCCCCAGNAGATCCTTTTCCAGTGACTTTGAAGAACAAACATNCTCAATACTCAAACT
  CAGTGTTTTTGACATAGCCAGGAGTGGTAAGGAAATCGTTTAGAATGACAAAACACCTGA
  CGCTGTGCAGCATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016218 unedited
  GGCCGCATTTCTANATCGAGTTTTTTTTTTTTTTTTTTATCGTTTTAGTAGAGACAGGGT
  CTCACCATGTTGGCGAGGCTGGTCTCAAACTCCTGACCTCAAGTGATCTGCCCATTCGGC
  CTCCCAAAGGGCTGGCATTACAGGTGTGAGCCATTGTGCCTGGCCTAACTAATTATTTTT
  TGAGAAGAACTCATTGATAGCACAAAAGTACTTGGAGAAACTACTTAAGCTAACTACTAT
  TAAGCTGTCTTCTTTCACAAATATTAATAAAAGTACTGATAGAAATGTAACTAAGATACA
  TAATGTGTTTTAATACACATTAATCTTTTGAATTACACATTTTCTCTTGAGGATTATAAT
  AAAATGATGGTACAATTTATCCTTCTTTATAGATTTGTGGAAAGTACCAACATAATTGAC
  AAGAAATGAAATACTGCCATTTAAAAGTCCTGTATTAAAATAAAACTACTTTGAATCATC
  TTAGACTAAATATTTTAAAAACTGATTCAAGTGCTTATTCATAAGATAATGGAATTTTAA
  ATAAGAAAAGCCAAATTAAAAATTATATTTTAGGCAAAAACCATTTTAACACAGATAATA
  CTGTGATATTTATTTTCCTGAAGAAAACCACTTATGCTAGTTACATTAATACTCTTCATA
  GAACTTAAGATAAATTCTATTATGAATGCTCATGAAGTATCTAAAGACACTAGGATCAAT
  GCCAACANAGTAGTTAAGGGCTGAAAATTCTTATGATATATAGGCATGTGAGAATCTTTG
  AGAGCCCTACAAAGTTTTTATTTCAGTTAAAATTATAAAAAATCACTTTCTTTCCCATTT
  CTAATTCCCCTTTAAGAATTTATCCTTAACAGATTGATTTCCATTGATGTTTATAGAAGG
  GAAATTCTCATGGCTATAAAAGAACCCCGAATCCGTTTGTTTCTTAAAAAGCACCTCTCT
  GGGAAGGTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016218
• ORF Size: 2613 bp
• Insert Size: 3900
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_016218.1, NP_057302.1
• RefSeq Size: 4074
• RefSeq ORF: 2613
• Locus ID: 51426
• Domains: IMS, ZnF_Rad18
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the DNA polymerase type-Y family of proteins. The encoded protein is a specialized DNA polymerase that catalyzes translesion DNA synthesis, which allows DNA replication in the presence of DNA lesions. Human cell lines lacking a functional copy of this gene exhibit impaired genome integrity and enhanced susceptibility to oxidative damage. Mutations in this gene that impair enzyme activity may be associated with prostate cancer in human patients. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.