GFM1 (NM_024996) Human Untagged Clone

CAT#: SC111592

GFM1 (untagged)-Human G elongation factor, mitochondrial 1 (GFM1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GFM1
• Synonyms: COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1; mtEF-G1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111592 sequence for NM_024996 edited (data generated by NextGen Sequencing)
  ATGAGACTCCTGGGAGCTGCAGCCGTCGCGGCTCTGGGGCGCGGAAGGGCCCCCGCCTCC
  CTAGGCTGGCAGAGGAAGCAGGTTAATTGGAAGGCCTGCCGATGGTCTTCATCAGGGGTG
  ATTCCTAATGAAAAAATACGAAATATTGGAATCTCAGCTCACATTGATTCTGGGAAAACT
  ACATTAACAGAACGAGTCCTTTACTACACTGGCAGAATTGCAAAGATGCATGAGGTGAAA
  GGTAAAGATGGAGTTGGTGCTGTCATGGATTCCATGGAACTAGAGAGACAAAGAGGAATC
  ACTATTCAGTCAGCAGCCACTTACACCATGTGGAAAGATGTCAATATTAACATTATAGAT
  ACTCCTGGGCATGTGGACTTCACAATAGAAGTGGAAAGGGCCCTGAGAGTGTTGGATGGT
  GCAGTCCTTGTTCTCTGTGCTGTTGGAGGGGTACAGTGCCAGACCATGACTGTCAATCGT
  CAGATGAAGCGCTACAACGTTCCGTTTCTAACTTTTATTAACAAATTGGACCGAATGGGC
  TCCAACCCAGCCAGGGCCCTGCAGCAAATGAGGTCTAAACTAAATCATAATGCAGCGTTT
  ATGCAGATACCCATGGGTTTGGAGGGTAATTTTAAAGGTATTGTAGATCTTATTGAGGAA
  CGAGCCATCTATTTTGATGGAGACTTTGGTCAGATTGTTCGATATGGTGAGATTCCAGCT
  GAATTAAGGGCGGCGGCCACTGACCACCGGCAGGAGCTAATTGAATGTGTTGCCAATTCA
  GATGAACAGCTTGGTGAGATGTTTCTGGAAGNNAAAATCCCCTCGATTTCTGATTTAAAG
  CTAGCAATTCGAAGAGCTACTCTGAAAAGATCATTTACTCCTGTATTTTTGGGAAGCGCC
  TTGAAGAACAAAGGAGTTCAGCCTCTTTTAGATGCTGTTTTAGAATACCTCCCAAATCCA
  TCTGAAGTCCAGAACTATGCTATTCTCAATAAAGAGGATGACTCAAAAGAGAAAACCAAA
  ATCCTAATGAACTCCAGTAGAGACAATTCCCACCCATTTGTAGGCCTGGCTTTTAAACTG
  GAGGTAGGTCGATTTGGACAATTAACTTATGTTCGCAGTTATCAGGGAGAGCTAAAGAAG
  GGTGACACCATCTATAACACAAGGACAAGAAAGAAAGTACGGTTGCAACGGCTGGCTCGC
  ATGCATGCCGACATGATGGAGGATGTTGAGGAAGTATATGCCGGAGACATCTGTGCATTG
  TTTGGCATTGACTGTGCTAGTGGAGACACATTCACAGACAAAGCCAACAGCGGCCTTTCT
  ATGGAGTCAATTCATGTTCCTGATCCTGTCATTTCAATAGCAATGAAGCCTTCTAACAAG
  AACGATCTGGAAAAATTTTCAAAAGGTATTGGCAGGTTTACAAGAGAAGATCCCACATTT
  AAAGTATACTTTGACACTGAGAACAAAGAGACAGTTATATCTGGAATGGGAGAATTACAC
  CTGGAAATCTATGCTCAGAGGCTGGAAAGAGAGTATGGCTGTCCTTGTATCACAGGAAAG
  CCAAAAGTTGCCTTTCGAGAGACCATTACTGCCCCTGTCCCGTTTGACTTTACACATAAA
  AAACAATCAGGTGGTGCAGGCCAGTATGGAAAAGTAATAGGTGTCCTGGAGCCTCTGGAC
  CCAGAGGACTACACTAAATTGGAATTTTCAGATGAAACATTCGGATCAAATATTCCAAAG
  CAGTTTGTGCCTGCTGTAGAAAAGGGGTTTTTAGATGCCTGCGAGAAGGGCCCTCTTTCT
  GGTCACAAGCTCTCTGGGCTCCGGTTTGTCCTGCAAGATGGAGCACACCACATGGTTGAT
  TCTAATGAAATCTCTTTCATCCGAGCAGGAGAAGGTGCTCTTAAACAAGCCTTGGCAAAT
  GCAACATTATGTATTCTTGAACCTATTATGGCTGTGGAAGTTGTAGCTCCAAATGAATTT
  CAGGGACAAGTAATTGCAGGAATTAACCGACGCCATGGGGTAATCACTGGGCAAGATGGA
  GTTGAGGACTATTTTACACTGTATGCAGATGTCCCTCTAAATGATATGTTTGGTTATTCC
  ACTGAACTTAGGTCATGCACAGAGGGAAAGGGAGAATACACAATGGAGTATAGCAGGTAT
  CAGCCATGTTTACCATCCACACAAGAAGACGTCATTAATAAGTATTTGGAAGCTACAGGT
  CAACTTCCTGTTAAAAAAGGAAAAGCCAAGAACTAA
  
  Clone variation with respect to NM_024996.5
  812 a=>n;813 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024996 unedited
  AGGCATATTTGTNAATACGCACCCACTATAGGGCGCGCCGCGAATTCGCACGAGGCGGCG
  CCACGGGACTTTGACGCGTGCTCTGCGCTTGCCATGAGACTCCTGGGAGCTGCAGCCGTC
  GCGGCTCTGGGGCGCGGAAGGGCCCCCGCCTCCCTAGGCTGGCAGAGGAAGCAGGTTAAT
  TGGAAGGCCTGCCGATGGTCTTCATCAGGGGTGATTCCTAATGAAAAAATACGAAATATG
  GGAATCTCAGCTCACATTGATTCTGGGAAAACTACATTAACAGAACGAGTCCTTTACTAC
  ACTGGCAGAATTGCAAAGATGCATGAGGTGAAAGGTAAAGATGGAGTTGGTGCTGTCATG
  GATTCCATGGAACTAGAGAGACAAAGAGGAATCACTATTCAGTCAGCAGCCACTTACACC
  ATGTGGAAAGATGTCAATATTAACATTATAGATACTCCTGGGCATGTGGACTTCACAATA
  GAAGTGGAAAGGGCCCTGAGAGTGTTGGATGGTGCAGTCCTTGTTCTCTGTGCTGTTGGA
  GGGGTACAGTGCCAGACCATGACTGTCAATCGTCAGATGAAGCGCTACAACGTTCCGTTT
  CTAACTTTTATTAACAAATTGGACCGAATGGGCTCCAACCCAGCCAGGGCCCTGCAGCAA
  ATGAGGTCTAAACTCAATCATAATGCAGCGTTTATGCAAATACCCATGGGTTTGGAGGGT
  AATTTTAAAGGTATTGTAAATCTTATTGAGGAACGAGCCATCTATTTTGATGGAGACCTT
  TGTCANATTGTTCGAATGGTGAGATCCACCTGAAATAGGGGCGCNGCCACTGACCACGGN
  CGGGGCTAATTGAATGGGTTGCCATTAAAAGACACCTTGTGAGATGTTCGGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024996 unedited
  CTTTGAACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTCCAAATTAAACTAGCATTCATTAGGTTGATGAAACTAACTCTATAA
  ATTACATTTGATTTACCTGTCCAAAAACTTTAAGATGAACACAAACAAGAATAGTTTATG
  CAAGGGTCTACTTTTTACTTCACAATAAACCACAGGGGAAGTNAATACTTATACTCCTAA
  AACAGAGGCAGTTACAAATTTGCAGAAACCACAAACTGAAATAAACAGACTTTTTTAAAA
  CAGAGAGAAATTGGGCTAATATTTTAATATCCCAATGAAGAATCTGAAAACACTCTCTAT
  GAAGAAATACGGAATCCACTCTTCAACAGAAAAGCCTGCCCATTAATGACACTCCAAGAA
  GTTCATGATATATGCGATTCACTTTCAAGTCTCAGCAAACTAAAAATAGAGTNTTGAGAC
  TTAAACAGATAATGGACGGTGAAAGCAAATNTGACAAAACAAATATTATTTGATAAACAA
  CCCGTTAACAAGGAAGTANNATGAAAGTTGTGGTATCCTTTGCATTTTTCAGCTTTCTCT
  ATGTGAAACTCTTATGATCAAAATTNGAAAATGACTACATTCTAAGCTAAAAGTTTACTG
  AAATAGTCTCTTTCCCCTAAATATTAACATTTTCAGNATAATATTTTTAATTGGGGATTT
  TCATGACTTTAAACCAATCATTGAGTGGAAATGACTAGAATACCTTTGAATTAACAACCC
  TCTGAATTAACAAAAAGGCTTTTTTGAAGAAAAAAACCCGTTTCTGGGCTAAAATTTTTG
  TTTAACACCCGAATTTTTCCCTGGATCCCTCAAGGTTAAACCCCCCATTCATTTAGGTCG
  CCACTCCATAGCAAGTTATTTTTGCTTTCCTTTTAAAAGAAAGGCCCGTACTCCAATCTT
  TAAAA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_024996
• ORF Size: 2256 bp
• Insert Size: 3110
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_024996.5, NP_079272.4
• RefSeq Size: 3468
• RefSeq ORF: 2256
• Locus ID: 85476
• Domains: EFG_C, GTP_EFTU, GTP_EFTU_D2, EFG_IV
• Gene Summary: Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter, compared to isoform 1.