GFM1 (NM_024996) Human Untagged Clone

CAT#: SC324018

GFM1 (untagged)-Human G elongation factor, mitochondrial 1 (GFM1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GFM1
• Synonyms: COXPD1; EFG; EFG1; EFGM; EGF1; GFM; hEFG1; mtEF-G1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_024996.5 CTTCCCGGTGCGTTACCGGCAGCTGAACCCACCCGGCGCCACGGGACTTTGACGCGTGCT
  CTGCGCTTGCCATGAGACTCCTGGGAGCTGCAGCCGTCGCGGCTCTGGGGCGCGGAAGGG
  CCCCCGCCTCCCTAGGCTGGCAGAGGAAGCAGGTTAATTGGAAGGCCTGCCGATGGTCTT
  CATCAGGGGTGATTCCTAATGAAAAAATACGAAATATTGGAATCTCAGCTCACATTGATT
  CTGGGAAAACTACATTAACAGAACGAGTCCTTTACTACACTGGCAGAATTGCAAAGATGC
  ATGAGGTGAAAGGTAAAGATGGAGTTGGTGCTGTCATGGATTCCATGGAACTAGAGAGAC
  AAAGAGGAATCACTATTCAGTCAGCAGCCACTTACACCATGTGGAAAGATGTCAATATTA
  ACATTATAGATACTCCTGGGCATGTGGACTTCACAATAGAAGTGGAAAGGGCCCTGAGAG
  TGTTGGATGGTGCAGTCCTTGTTCTCTGTGCTGTTGGAGGGGTACAGTGCCAGACCATGA
  CTGTCAATCGTCAGATGAAGCGCTACAACGTTCCGTTTCTAACTTTTATTAACAAATTGG
  ACCGAATGGGCTCCAACCCAGCCAGGGCCCTGCAGCAAATGAGGTCTAAACTAAATCATA
  ATGCAGCGTTTATGCAGATACCCATGGGTTTGGAGGGTAATTTTAAAGGTATTGTAGATC
  TTATTGAGGAACGAGCCATCTATTTTGATGGAGACTTTGGTCAGATTGTTCGATATGGTG
  AGATTCCAGCTGAATTAAGGGCGGCGGCCACTGACCACCGGCAGGAGCTAATTGAATGTG
  TTGCCAATTCAGATGAACAGCTTGGTGAGATGTTTCTGGAAGAAAAAATCCCCTCGATTT
  CTGATTTAAAGCTAGCAATTCGAAGAGCTACTCTGAAAAGATCATTTACTCCTGTATTTT
  TGGGAAGCGCCTTGAAGAACAAAGGAGTTCAGCCTCTTTTAGATGCTGTTTTAGAATACC
  TCCCAAATCCATCTGAAGTCCAGAACTATGCTATTCTCAATAAAGAGGATGACTCAAAAG
  AGAAAACCAAAATCCTAATGAACTCCAGTAGAGACAATTCCCACCCATTTGTAGGCCTGG
  CTTTTAAACTGGAGGTAGGTCGATTTGGACAATTAACTTATGTTCGCAGTTATCAGGGAG
  AGCTAAAGAAGGGTGACACCATCTATAACACAAGGACAAGAAAGAAAGTACGGTTGCAAC
  GGCTGGCTCGCATGCATGCCGACATGATGGAGGATGTTGAGGAAGTATATGCCGGAGACA
  TCTGTGCATTGTTTGGCATTGACTGTGCTAGTGGAGACACATTCACAGACAAAGCCAACA
  GCGGCCTTTCTATGGAGTCAATTCATGTTCCTGATCCTGTCATTTCAATAGCAATGAAGC
  CTTCTAACAAGAACGATCTGGAAAAATTTTCAAAAGGTATTGGCAGGTTTACAAGAGAAG
  ATCCCACATTTAAAGTATACTTTGACACTGAGAACAAAGAGACAGTTATATCTGGAATGG
  GAGAATTACACCTGGAAATCTATGCTCAGAGGCTGGAAAGAGAGTATGGCTGTCCTTGTA
  TCACAGGAAAGCCAAAAGTTGCCTTTCGAGAGACCATTACTGCCCCTGTCCCGTTTGACT
  TTACACATAAAAAACAATCAGGTGGTGCAGGCCAGTATGGAAAAGTAATAGGTGTCCTGG
  AGCCTCTGGACCCAGAGGACTACACTAAATTGGAATTTTCAGATGAAACATTCGGATCAA
  ATATTCCAAAGCAGTTTGTGCCTGCTGTAGAAAAGGGGTTTTTAGATGCCTGCGAGAAGG
  GCCCTCTTTCTGGTCACAAGCTCTCTGGGCTCCGGTTTGTCCTGCAAGATGGAGCACACC
  ACATGGTTGATTCTAATGAAATCTCTTTCATCCGAGCAGGAGAAGGTGCTCTTAAACAAG
  CCTTGGCAAATGCAACATTATGTATTCTTGAACCTATTATGGCTGTGGAAGTTGTAGCTC
  CAAATGAATTTCAGGGACAAGTAATTGCAGGAATTAACCGACGCCATGGGGTAATCACTG
  GGCAAGATGGAGTTGAGGACTATTTTACACTGTATGCAGATGTCCCTCTAAATGATATGT
  TTGGTTATTCCACTGAACTTAGGTCATGCACAGAGGGAAAGGGAGAATACACAATGGAGT
  ATAGCAGGTATCAGCCATGTTTACCATCCACACAAGAAGACGTCATTAATAAGTATTTGG
  AAGCTACAGGTCAACTTCCTGTTAAAAAAGGAAAAGCCAAGAACTAACTTTGCTTACTGT
  GAGTTGACTGACTCTAATTGAATCTGCGTGGTTTTGATACTTTGATGGATTCCAGTGGAA
  TAAATTCAGGCTGCTGAAACAAGAAATTCTGAGCCCAGGAAGCGGGCTCTTCTTTCTTCA
  AAAGAAGCCCTTCTTGTTCATATTCAGGAGCTTCTGTTATATTCAAAGGTAATTCTATGT
  CTATCTCAACTCTATTGATTGGTTTTATAGTTCATTGAAAATCCTCAAATAAAATATAAT
  TATTACTGAAATATGTTTAATATTTAAGGGGAAAAGAGACTAATTTCAGTTATACTTTTA
  AGCTTAGAATGTATGTTCATTTCCAAATTTTGTATCATAAGAGTTTTCAACATAGAGAAA
  AGCTGAAAAAATGCAAAGAATAACCACATACTTTCCATCTACCTTCCTTTGTTAACGGGT
  TGTTTATCATATAATAATTTGTTTTGTCATATTTGCTTTCACTGTCTATTATCTGTTTAA
  GTCTCATAACTCTATTTTTAGTTTGCTGAAGACTTGAAAGTGAATCGCATATATCATGAC
  ACTTCTTGGAGTGTCATTAATGGGCAGGCTTTTCTGTTGAAGAGTGGATTCCGTATGTTC
  TTCATAGAGAGTGTTTTTCAGATTCTTCATTGGGATATTAAAATATTAGCCAAAAAAAAA
  AAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024996.5 unedited
  CTCCATAGCGGTACACGAACCTACTACTAGGGCGGCAGGCGACTCGCGGCGCGCCGGTAC
  CGGTCCGGAATTCCCGGGATATCGTCGACCCACGCGTCCGCTTCCCGGTGCGTTACCGGC
  AGCTGAACCCACCCGGCGCCACGGGACTTTGACGCGTGCTCTGCGCTTGCCATGAGACTC
  CTGGGAGCTGCAGCCGTCGCGGCTCTGGGGCGCGGAAGGGCCCCCGCCTCCCTAGGCTGG
  CAGAGGAAGCAGGTTAATTGAAAACCCTGTTTCTTGTCTAGTTGATGATGACCCATGCTC
  TGGAGTGTCCCAGCTTAACTGACCACCGGTTTCCAGAATTAGACAAACGATAGGGGGATG
  ATTTCAGCCTTCACCCACTTGTCCCGGGGACATACACATCTTATGAACAAAACACACCTT
  GATATATTTAGTGAGCCTCTCCCTAATGATGATTCTCACCTTGATGCGGCCAACTATTAC
  GGAGACATCAGAAAAATTCCCCTAGTTTCCCCAACTTA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_024996
• ORF Size: 2256 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_024996.5, NP_079272.4
• RefSeq Size: 3468
• RefSeq ORF: 2256
• Locus ID: 85476
• Domains: EFG_C, GTP_EFTU, GTP_EFTU_D2, EFG_IV
• Gene Summary: Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter, compared to isoform 1.