ATP6V1G2 (NM_130463) Human Untagged Clone

CAT#: SC111594

ATP6V1G2 (untagged)-Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2 (ATP6V1G2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP6V1G2
• Synonyms: ATP6G; ATP6G2; NG38; VMA10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111594 sequence for NM_130463 edited (data generated by NextGen Sequencing)
  ATGGCCAGTCAGTCCCAAGGTATCCAGCAGCTTCTGCAAGCTGAGAAGCGGGCAGCTGAG
  AAGGTGGCAGATGCCAGAAAGAGGAAGGCCCGGCGACTGAAGCAGGCAAAGGAGGAGGCA
  CAGATGGAGGTGGAGCAATACCGCAGAGAGCGAGAGCACGAATTCCAGAGCAAGCAGCAG
  GCGGCCATGGGCTCCCAGGGGAACCTGTCTGCTGAGGTGGAGCAGGCTACAAGGCGCCAG
  GTGCAGGGCATGCAGAGCTCCCAGCAGAGAAACCGAGAGCGTGTCCTGGCCCAGCTTCTT
  GGCATGGTCTGCGACGTCAGGCCCCAGGTCCACCCCAACTACCGGATTTCTGCCTAG
  
  Clone variation with respect to NM_130463.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_130463 unedited
  GTAACGTCAAATTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCAGA
  AATGGCCAGTCAGTCCCAAGTATCCAGCAGCTTCTGCAAGCTGAGAAGCGGGCAGCTGAG
  AAGGTGGCAGATGCCAGAAAGAGGAAGGCCCGGCGACTGAAGCAGGCAAAGGAGGAGGCA
  CAGATGGAGGTGGAGCAATACCGCAGAGAGCGAGAGCACGAATTCCAGAGCAAGCAGCAG
  GCGGCCATGGGCTCCCAGGGGAACCTGTCTGCTGAGGTGGAGCAGGCTACAAGGCGCCAG
  GTGCAGGGCATGCAGAGCTCCCAGCAGAGAAACCGAGAGCGTGTCCTGGCCCAGCTTCTT
  GGCATGGTCTGCGACGTCAGGCCCCAGGTCCACCCCAACTACCGGATTTCTGCCTAGGGC
  CACCGTAGGGCCTGACTCCTTCTGCCAGTTCCCTCCCTCAAAGAAATCCTCCAATCAAAA
  TCACCTCCCACCATAATCCCTGTCTTCTTTCCATCCCCTAGAAATCCTGGGAGGCAGGAT
  CCAATAATTTTCCTGTGACACTTATAAATATCCTGCTCACATCTGAATCTCCTTGTTGTT
  CTTTAACCCTCACTGGGACTTTGTAAACTTCCAAGTCATTCTCACCTAAACCCTCTGTGA
  AATTTGTAATATGGGGAAGTAGGAATGTGGAAAACATCCTGACTTCAGTGTCTGGCCGAT
  GTGGGTCCCTCTCTTGACCCTGTCACTTTGCTGCTGTGAAACCAGGACAAGCTACTTAAC
  TTGGTAGCCTCGATGTCCTCCTTTTGTGAAACTGGGGATGATTATAATGCCTACCTTGTG
  GAGGGTTGCTTCAATGATTTAGGATCATCCTGTAAAGTCTAACACAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_130463 unedited
  TTCAACTATGNNACCGCGGCCGCATGCTAGNGATCGATTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCACCTCCCCAATTTTTTTGCC
  CAATAATCATTTTATTGATCCTTTTTTTATTGTTCCAAGGGGAAAAGTAAGGGGTCAAGG
  ATTTAAAAAGGAAGGGCTTCCTTTTAAGGGAACCCAGTTTCTTTTTAAATTTTAAAAAGC
  AATGACCTTTTGATAATTTTTTGGGGAAATAGGAAAGGGGGAAAATTTTTTAAAGCCAAA
  TTTCACCAAAAAAAAAACAAAGGGGGGCTCCAAAATGCCTTTTTTTCCCTGGAATTATAA
  AAAAGGGGCTAAATGTAGCCATCTCCTTTTTTTGGAAATCAAAAGGTCTTTGGAAAAAAA
  AAAAAACCAATTTTTCAAAAAATAACTGGGGTCACAAAATGAACAATTGGAATTAAAAAC
  CCCTTGATGGACTTGAGAAAACACCTTTTTAGGTTTTGACCATTGACCAGGTGGGGGTAA
  TAGTATCACAGGGTTGCTACTTACTGAATCACTGCTACAACATGCAAGGAACTGTGCTAA
  ACTTTACAAAATGATTCCTAATCATTGAACCAACCCTCACAAGGTAGGCTTTATTATCAT
  CCCAGTTTCACAAAGGAGGACATCGGGGCTACCAAGTTAAGTAGCTTGTCCTGGTTTCCA
  GCCACCAAGTGACAGGGTCAAAAGAGGGACCCCAATGGGCCAGACACTGAATCAGGATGT
  TTTCCACATCCTACTTCCCCTTTTTACAATTTTCACAAAGGTTTTAAGTGAAAAAGACTT
  GGAAATTTACAAAGTCCCGTGGGGGTTAAAGAAACACAGGAAATTCCATTGTGACCGGGT
  TTTTTTAAGTTCCCAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_130463
• Insert Size: 1250 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130463.2, NP_569730.1
• RefSeq Size: 1594 bp
• RefSeq ORF: 357 bp
• Locus ID: 534
• Cytogenetics: 6p21.33
• Domains: V-ATPase_G
• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
• Gene Summary: 'This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of three V1 domain G subunit proteins. This gene had previous gene symbols of ATP6G and ATP6G2. Alternatively spliced transcript variants encoding different isoforms have been described. Read-through transcription also exists between this gene and the downstream DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B (DDX39B) gene. [provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).