CD8A (NM_001768) Human Untagged Clone

CAT#: SC111602

CD8A (untagged)-Human CD8a molecule (CD8A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD8A
• Synonyms: CD8; Leu2; p32
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111602 sequence for NM_001768 edited (data generated by NextGen Sequencing)
  ATGGCCTTACCAGTGACCGCCTTGCTCCTGCCGCTGGCCTTGCTGCTCCACGCCGCCAGG
  CCGAGCCAGTTCCGGGTGTCGCCGCTGGATCGGACCTGGAACCTGGGCGAGACAGTGGAG
  CTGAAGTGCCAGGTGCTGCTGTCCAACCCGACGTCGGGCTGCTCGTGGCTCTTCCAGCCG
  CGCGGCGCCGCCGCCAGTCCCACCTTCCTCCTATACCTCTCCCAAAACAAGCCCAAGGCG
  GCCGAGGGGCTGGACACCCAGCGGTTCTCGGGCAAGAGGTTGGGGGACACCTTCGTCCTC
  ACCCTGAGCGACTTCCGCCGAGAGAACGAGGGCTACTATTTCTGCTCGGCCCTGAGCAAC
  TCCATCATGTACTTCAGCCACTTCGTGCCGGTCTTCCTGCCAGCGAAGCCCACCACGACG
  CCAGCGCCGCGACCACCAACACCGGCGCCCACCATCGCGTCGCAGCCCCTGTCCCTGCGC
  CCAGAGGCGTGCCGGCCAGCGGCGGGGGGCGCAGTGCACACGAGGGGGCTGGACTTCGCC
  TGTGATATCTACATCTGGGCGCCCTTGGCCGGGACTTGTGGGGTCCTTCTCCTGTCACTG
  GTTATCACCCTTTACTGCAACCACAGGAACCGAAGACGTGTTTGCAAATGTCCCCGGCCT
  GTGGTCAAATCGGGAGACAAGCCCAGCCTTTCGGCGAGATACGTCTAA
  
  Clone variation with respect to NM_001768.6
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001768 unedited
  AGTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGCTCCCGCG
  CGCCTCCCCTCGCGCCCGAGCTTCGAGCCAAGCAGCGTCCTGGGGAGCGCGTCATGGCCT
  TACCAGTGACCGCCTTGCTCCTGCCGCTGGCCTTGCTGCTCCACGCCGCCAGGCCGAGCC
  AGTTCCGGGTGTCGCCGCTGGATCGGACCTGGAACCTGGGCGAGACAGTGGAGCTGAAGT
  GCCAGGTGCTGCTGTCCAACCCGACGTCGGGCTGCTCGTGGCTCTTCCAGCCGCGCGGCG
  CCGCCGCCAGTCCCACCTTCCTCCTATACCTCTCCCAAAACAAGCCCAAGGCGGCCGAGG
  GGCTGGACACCCAGCGGTTCTCGGGCAAGAGGTTGGGGGACACCTTCGTCCTCACCCTGA
  GCGACTTCCGCCGAGAGAACGAGGGCTACTATTTCTGCTCGGCCCTGAGCAACTCCATCA
  TGTACTTCAGCCACTTCGTGCCGGTCTTCCTGCCAGCGAAGCCCACCACGACGCCAGCGC
  CGCGACCACCAACACCGGCGCCCACCATCGCGTCGCAGCCCCTGTCCCTGCGCCCAGAGG
  CGTGCCGGCCAGCGGCGGGGGGCGCAGTGCACACGAAGGGGCTGGACTTCGCCTGTGATA
  TCTACATCTGGGCGCCCTTGGCCGGGACTTNGTGGGTCCTTCTCCTGTCACTGGNTATCA
  CCCTTTACTGNCACCACAGGNAACCCGAGACGTGTTTGCAAATGTCCCCCGGCCTGTGGT
  CAAATCGGNAGACAAGCCCAGCCTTTCNGCGAGATACGTCTAACCCTGTGCACAGCCACT
  ACATTACTTTCAACTGAGATCCTTCCCTTTTGAGGGGAGCAGTNCNTTCCCTTTCATTNT
  TTNCCAGGCTNCCTCCTGGGNATNCATTCTCATGAATATTAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001768 unedited
  ACCGCGGCCGCATTTTANAGTCGAGTTTTTTTTTTTTTTTTTTTGCTGTTTACCCATTAA
  AGAGGATGCTACAAATGTTTATTCATTAAAGCCACTCATAACAGCATAGTACAACCCTAT
  TTAAAAAAGAAAGACATATTTTACATGTATGTGTAGAAAATAATCAGGAAGAATGTACAC
  CATATTAACAGGAGATTATTCCTAAGGTGAAGAATTATGAGTGGTTCTTATTTCCTTATC
  TACTTAAGTTTTTTCTCTCCAATGGGCAGGCATTGCTTGTACCATACCTTAAGCAAGGAA
  GTGCATGTTTGGGACAGCAGTTTGGGCTCTCAGCCTCCTTAAGAGAGTCAGGTCTGCCTC
  ATCCCTGTATCTGCTAGTTGAGCAGAGGCCAGGGCTGTGTGAGGGGCTCTCCAACAATTG
  TCTTTACAAAGTATAAAAAGTCATCAGTGATCCCAGGAACATGTTTGTATCTCAAATTCA
  GAGATTCAAGAGGGCCTTAGTTTAACCTCACTGATGCTCAAATTCTATTTGTAAAGGGGT
  AGCCTGTCCTCTTTCATGGGCCCCTCTGCAATGCAAGGGCTGGGAGAGCAATTCCGCCTC
  CACATAGGGGTTTCACAGAGATTTTCTTTAGAGATAGAGGGATTCATTTTCCAGGGTTAA
  GCTCACCACTTCATTTTATTTTAGGTCTATACAATTNTAAGCTCGATTATTAAAAAAAAA
  AGTCTGATATTGTTTACATTATAGAACTTTGCCAAAGGCAGCNCTCATTCTTTATTCATT
  ACCTCCTTGAGGCTTTGGCACAGTATCCCAAGTATTAAGAAGGACTTGTTCCCTTGCCGT
  TGGGACTTAAGCCCCTCACCTGAAACAGGGCCTCTGAAAGAAGCACCGAATGTGTGGAGG
  AAGAACCCTGACTTGGAAGACAAGTCCCTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001768
• Insert Size: 2210 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001768.4, NP_001759.3
• RefSeq Size: 2261 bp
• RefSeq ORF: 708 bp
• Locus ID: 925
• Cytogenetics: 2p11.2
• Domains: ig, IGv, IG
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Antigen processing and presentation, Cell adhesion molecules (CAMs), Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway
• Gene Summary: 'The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020]'
  Transcript Variant: This variant (1) encodes the longer, membrane associated isoform (1). Both variants 1 and 3 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.