CNN2 (NM_004368) Human Untagged Clone

CAT#: SC111610

CNN2 (untagged)-Human calponin 2 (CNN2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CNN2
• Synonyms: calponin 2; calponin H2, smooth muscle; neutral calponin
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111610 sequence for NM_004368 edited (data generated by NextGen Sequencing)
  ATGAGCTCCACGCAGTTCAACAAGGGCCCCTCGTACGGGCTGTCGGCCGAGGTCAAGAAC
  CGGCTCCTGTCCAAATATGACCCCCAGAAGGAGGCAGAGCTCCGCACCTGGATCGAGGGA
  CTCACCGGCCTCTCCATCGGCCCCGACTTCCAGAAGGGCCTGAAGGATGGAACTATCTTA
  TGCACACTCATGAACAAGCTACAGCCGGGCTCCGTCCCCAAGATCAACCGCTCCATGCAG
  AACTGGCACCAGCTAGAAAACCTGTCCAACTTCATCAAGGCCATGGTCAGCTACGGCATG
  AACCCTGTGGACCTGTTCGAGGCCAACGACCTGTTTGAGAGTGGGAACATGACGCAGGTG
  CAGGTGTCTCTTCTCGCCCTGGCGGGGAAGGCCAAGACTAAGGGGCTGCAGAGCGGGGTG
  GACATTGGCGTCAAGTACTCGGAGAAGCAGGAGCGGAATTTCGACGATGCCACCATGAAG
  GCTGGCCAGTGCGTCATCGGGCTGCAGATGGGCACCAACAAATGCGCCAGCCAGTCGGGC
  ATGACTGCCTACGGCACGAGAAGGCATCTCTATGACCCCAAGAACCATATCCTGCCCCCC
  ATGGACCACTCGACCATCAGCCTCCAGATGGGCACGAACAAGTGCGCCAGCCAGGTGGGC
  ATGACGGCTCCCGGGACCCGGCGGCACATCTATGATACCAAGCTGGGAACCGACAAGTGT
  GACAACTCCTCCATGTCCCTGCAGATGGGCTACACGCAGGGCGCCAACCAGAGCGGCCAG
  GTCTTCGGCCTGGGCCGGCAGATATATGACCCCAAGTACTGCCCGCAAGGCACAGTGGCC
  GATGGGGCTCCCTCGGGCACCGGCGACTGCCCGGACCCGGGGGAGGTCCCTGAATATCCC
  CCTTACTACCAGGAGGAGGCCGGCTACTGA
  
  Clone variation with respect to NM_004368.2
  645 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004368 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGCGGCCCCGTCCCG
  CCGCCCGCCCGCCAGCCATGAGCTCCACGCAGTTCAACAAGGGCCCCTCGTACGGGCTGT
  CGGCCGAGGTCAAGAACCGGCTCCTGTCCAAATATGACCCCCAGAAGGAGGCAGAGCTCC
  GCACCTGGATCGAGGGACTCACCGGCCTCTCCATCGGCCCCGACTTCCAGAAGGGCCTGA
  AGGATGGAACTATCTTATGCACACTCATGAACAAGCTACAGCCGGGCTCCGTCCCCAAGA
  TCAACCGCTCCATGCAGAACTGGCACCAGCTAGAAAACCTGTCCAACTTCATCAAGGCCA
  TGGTCAGCTACGGCATGAACCCTGTGGACCTGTTCGAGGCCAACGACCTGTTTGAGAGTG
  GGAACATGACGCAGGTGCAGGTGTCTCTTCTCGCCCTGGCGGGGAAGGCCAAGACTAAGG
  GGCTGCAGAGCGGGGTGGACATTGGCGTCAAGTACTCGGAGAAGCAGGAGCGGAATTTCG
  ACGATGCCACCATGAAGGCTGGCCAGTGCGTCATCGGGCTGCAGATGGGCACCAACAAAT
  GCGCCAGCCAGTCGGGCATGACTGCCTACNGCACGAGAAGGCATCTCTATGACCCCAAGA
  ACCATATCCTGCCCCCCATGGACCACTCGACCATCAGCCTCCAGATGGGCACGAACAAGT
  GCGCCAGCCANGTGGGCATGACGGCTCCCCGGACCCGCGGCACATCTATGATACCAAGCT
  GGAACCNNGACAGTGTGACAACTCCTTCATGTCCCTGCAGATGGGCTACACGCAGGGNCG
  CCAACCAGAGCGGCCCAGTCTTTCGCCTGGGCCCGGAAGATTATGACCCCAGTACTGGCC
  GCAGGCCCAGTGGCCGATGGGGCTCCCTTCGGCACCGGGACTGCCCGGACGGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004368 unedited
  CGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCTTCGCCAAACAGAGTTTTA
  TTGCGTTTGGTCCACAGTCGTATTTCACATTATCTCACGGAGCAGGGCCCCTGGGTGGGG
  GGTCCCTGTGCAGTACTTGGCGGGGCATGTGGCCGGGGAAGATGGAGCAGTATAAGCTGG
  TCGGGCCCAGAAGGGAGGAGGGCTGGGGACTCCTTAAAACCTACTGAGGGCCTGGGCACG
  GTGGCTCACACCTGTAATCTCAGCGCTTGGGGAGGCGGAGGCGGGCGGATCACACCTGAG
  GCCAGGAGTTCGAGACCAGCCTGGCCAACATGGGGAAACCCCATCTTTACTAAAAATACA
  AAAATTAGCCGGGCGTGGTGGAGCACCTGCAGTCCTAGCTACTCAGGAGGCTGAGGCTGG
  AGAATCGCTTGAGCCCGGAAGGTGGAGGTGCAGTGAGCCAAGATGGCGCCACTGCACTCC
  AGCCTGGGCAACAACCACAACTGAAAAAAAAATCTACTGAGGCCAGAGTGAGTTGGGGCG
  GGGAGCATGGAGAGGGGGTCAGGCTTATCCCCAACAACTCAGAATTTCACTTTCTTGTAC
  GGACCTCACTTTCTCCACCTGGCCCCGGGGCCAGGTCTGAAGGTCTGAAGTCTGTTGACC
  CGAAAGTCCTAGGCAAATCCACCCCCTCGGGAGCCGGAATATTTAACATACGGCCCGTCC
  CCTTTCCAGGGACTCATTCCTAAACTCTCTCACTGCCTTGCCCACCCTGGGGAAAAAAGA
  TTTTTCTTTGGAAAACTTCTGAACCTGCGGGCTAAAACCCAACTGCCAGCTGACAAAGGG
  GAAAAGCGAGGAACATAAACCGCGGGCCCGGTNCTCCACCACATCTTTTTCCACCAACCG
  AAGTGCACAAGACCTCCCTAACTTGGGATAGCCTACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004368
• Insert Size: 2150 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004368.2, NP_004359.1
• RefSeq Size: 2478 bp
• RefSeq ORF: 930 bp
• Locus ID: 1265
• Cytogenetics: 19p13.3
• Domains: calponin, CH
• Gene Summary: 'The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Several pseudogenes of this gene have been identified, and are present on chromosomes 1, 2, 3, 6, 9, 11, 13, 15, 16, 21 and 22. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]'
  Transcript Variant: This variant (1) uses an alternate in-frame splice site in the central coding region, compared to variant 4. The encoded isoform (a) is shorter than isoform d.