Doublecortin (DCX) (NM_000555) Human Untagged Clone

CAT#: SC111620

DCX (untagged)-Human doublecortin (DCX), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DCX
• Synonyms: DBCN; DC; LISX; SCLH; XLIS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000555, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAAACACTCCCCCTTCATAGTCATTGTACTGAAATGCAAAGACTGCTTCCTAAGCTGGAGATGCTAA
  CCTTGGGTAGCTCCTTCTGTTCTCTTCAAGGGGAATTTTGTCAGGCTATGGATTCATTTACAACTGTTAG
  TCATGTGGGCATGTGTGAGGAAACAGATGCCAGTTTTAATGTATTTAGCCCGAAGTTCCAATTTGATAGG
  AGCCACTGTCAGTCTCTGAGGTTCCACCAAAATATGGAACTTGATTTTGGACACTTTGACGAAAGAGATA
  AGACATCCAGGAACATGCGAGGCTCCCGGATGAATGGGTTGCCTAGCCCCACTCACAGCGCCCACTGTAG
  CTTCTACCGAACCAGAACCTTGCAGGCACTGAGTAATGAGAAGAAAGCCAAGAAGGTACGTTTCTACCGC
  AATGGGGACCGCTACTTCAAGGGGATTGTGTACGCTGTGTCCTCTGACCGTTTTCGCAGCTTTGACGCCT
  TGCTGGCTGACCTGACGCGATCTCTGTCTGACAACATCAACCTGCCTCAGGGAGTGCGTTACATTTACAC
  CATTGATGGATCCAGGAAGATCGGAAGCATGGATGAACTGGAGGAAGGGGAAAGCTATGTCTGTTCCTCA
  GACAACTTCTTTAAAAAGGTGGAGTACACCAAGAATGTCAATCCCAACTGGTCTGTCAACGTAAAAACAT
  CTGCCAATATGAAAGCCCCCCAGTCCTTGGCTAGCAGCAACAGTGCACAGGCCAGGGAGAACAAGGACTT
  TGTGCGCCCCAAGCTGGTTACCATCATCCGCAGTGGGGTGAAGCCTCGGAAGGCTGTGCGTGTGCTTCTG
  AACAAGAAGACAGCCCACTCTTTTGAGCAAGTCCTCACTGATATCACAGAAGCCATCAAACTGGAGACCG
  GGGTTGTCAAAAAACTCTACACTCTGGATGGAAAACAGGTAACTTGTCTCCATGATTTCTTTGGTGATGA
  TGATGTGTTTATTGCCTGTGGTCCTGAAAAATTTCGCTATGCTCAGGATGATTTTTCTCTGGATGAAAAT
  GAATGCCGAGTCATGAAGGGAAACCCATCAGCCACAGCTGGCCCAAAGGCATCCCCAACACCTCAGAAGA
  CTTCAGCCAAGAGCCCTGGTCCTATGCGCCGAAGCAAGTCTCCAGCTGACTCAGCAAACGGAACCTCCAG
  CAGCCAGCTCTCTACCCCCAAGTCTAAGCAGTCTCCCATCTCTACGCCCACCAGTCCTGGCAGCCTCCGG
  AAGCACAAGGACCTGTACCTGCCTCTGTCCTTGGATGACTCGGACTCGCTTGGTGATTCCATGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000555 unedited
  TTTTGTATACGACTCATATAGGCGGCNCGCGATTCGGCACGAGGGGCAAAGCCTGCTCTC
  TCTGTCTCTCTGTCTCCTCTTCTCCTTTTTTGCCTTATTCTATCCGATTTTTTCCCTAAG
  CTTCTACCTGGGATTTTCCTTTGGAAAAGTCTCTGAGGTTCCACCAAAATATGGAACTTG
  ATTTTGGACACTTTGACGAAAGAGATAAGACATCCAGGAACATGCGAGGCTCCCGGATGA
  ATGGGTTGCCTAGCCCCACTCACAGCGCCCACTGTAGCTTCTACCGAACCAGAACCTTGC
  AGGCACTGAGTAATGAGAAGAAAGCCAAGAAGGTACGTTTCTACCGCAATGGGGACCGCT
  ACTTCAAGGGGATTGTGTACGCTGTGTCCTCTGACCGTTTTCGCAGCTTTGACGCCTTGC
  TGGCTGACCTGACGCGATCTCTGTCTGACAACATCAACCTGCCTCAGGGAGTGCGTTACA
  TTTACACCATTGATGGATCCAGGAAGATCGGAACATGGATGAACTGGAGAAGGGAAAGCT
  TTGTCTGTTCCTCAGACAACTTCTTTAAAGGGTGGAGTACACCAAGAATGTAATCCCACT
  GGGTCTGTCAACGTAAAAACATCTGCCATATGAAAGCCCCCCAGTCCTTGGCTAGCAGCA
  ACAGTGCACAGGCCAGGGAGACNAGGNACTTTGTGCGCCCAAGCTGGTTACATATNCGAA
  NGGGGNGTGAGCCTCGAAAGTTTGNGCGTGTGCTTCTGACAAGAAACAGCCACTCTTTGG
  AGCAGTNCTACTGATTCACAGAGCATAACTGGACAGCGGGGGGGGTGCAAAAACTTACAT
  TCTGATGGAAACAGGTAACTGCTCTGATTTTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000555 unedited
  CTATGGACCGGGCGGCCGCATTCTAGNAGTCGAGTTTTTTTTTTTTTTTTTTGGTAAATT
  TTCTTCATGTGTTTTTTAGACAAAAGAAAAACACCAGTTTATAAAAAAAGGAGCCTGAAG
  AGTTTGCTCCTGAAATACAGACATGAATTTTTATAAAAAGGATATTGATTTCTTGCTATA
  AAAAGAGACTGAAAGGTGTCAGACCTGAAAACAAATTCTCACAGTGTTTTCAGTGTTGGC
  TGTTAACAAGGCATTCAATAGAACAGAGATAGATGGATACCATACAGTTCATGTAATCAT
  GTGGTATGCTAGACAGCCTTCTAGAGGAATCAGGTATATGAAGCCCCTTACTCCAAACTA
  TTATCAATTGCTCATTGATTCCAAATCGCCTCAGTGGAGAGATCGACCAGCACTTCACTG
  CGCTCTCCAAACTCTTCCCACCGTCCGCAGCCTTTCCCAATTCTGCCCCACTCCCTTATT
  TAACCCACCCCCCTTATCCCCCCCCTCTTGCCCTCCCCTCACTACTTCCCCCCCCCCCCT
  CCTCCCCTTCCCCCCCAAACCCGTTCTCGCCTCCCTCCCCCCTTCCCCCCCCCCCCCCCA
  CCCCCCCCCCCCCCCTTTCCCTCTTTCTCTTTCACGTTTTTCCCCCCTCCCTCTCCCCCC
  CCCTCTCTCTCCCCTCCCTCCCTCCCCTTTTCCCCCCCCCCACCCCGCCCCCCTCCCCCC
  CCCCCCCCTTGCCTCCCCCCCCCCCCCCCCCCTGTCCCGCCCCCCCCCCCTTTCTTCCCC
  TCCCTTCCCTCTTCTCCCCTCTCTTTCCCCCCCCCTTCCCCCGCTTGTGGCGCCCCCCCC
  CCNNNNNCCCCCCCCCNCCNNNCCNNCCCCCNNNNCCCCCCCCCCCCCCCCCNCCNCNCC
  CCCCCCCCNCCCCCCCNNCCCCCCCCCCNCCCCCNCNNCCCCCCNCCNCCNCNNNNNNNN
  CCCCCCCCCCCCNGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000555
• Insert Size: 2150 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000555.1, NP_000546.1
• RefSeq Size: 9433 bp
• RefSeq ORF: 1083 bp
• Locus ID: 1641
• Cytogenetics: Xq23
• Domains: DCX
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]'
  Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.