FGF1 (NM_000800) Human Untagged Clone

CAT#: SC111631

FGF1 (untagged)-Human fibroblast growth factor 1 (acidic) (FGF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FGF1
• Synonyms: AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-1; FGF-alpha; FGFA; GLIO703; HBGF-1; HBGF1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000800 edited
  CCGCCACATTATCTCCATTCCTCCAGTGCCCGCCTGACACTGGCCCTGAATCAGGGCTGG
  AGGGGGCAGGCATTTCTCATTTACTAAAGTGCTGGATGCAGCCCTTGAGGTTCGGCAGAA
  GCAGAAAGCTGCGTCTTGAAAGCGCCACAAGCAGCAGCTGCTGAGCCATGGCTGAAGGGG
  AAATCACCACCTTCACAGCCCTGACCGAGAAGTTTAATCTGCCTCCAGGGAATTACAAGA
  AGCCCAAACTCCTCTACTGTAGCAACGGGGGCCACTTCCTGAGGATCCTTCCGGATGGCA
  CAGTGGATGGGACAAGGGACAGGAGCGACCAGCACATTCAGCTGCAGCTCAGTGCGGAAA
  GCGTGGGGGAGGTGTATATAAAGAGTACCGAGACTGGCCAGTACTTGGCCATGGACACCG
  ACGGGCTTTTATACGGCTCACAGACACCAAATGAGGAATGTTTGTTCCTGGAAAGGCTGG
  AGGAGAACCATTACAACACCTATATATCCAAGAAGCATGCAGAGAAGAATTGGTTTGTTG
  GCCTCAAGAAGAATGGGAGCTGCAAACGCGGTCCTCGGACTCACTATGGCCAGAAAGCAA
  TCTTGTTTCTCCCCCTGCCAGTCTCTTCTGATTAAAGAGATCTGTTCTGGGTGTTGACCA
  CTCCAGAGAAGTTTCGAGGGGTCCTCACCTGGTTGACCCAAAAATGTTCCCTTGACCATT
  GGCTGCGCTAACCCCCAGCCCACAGAGCCTGAATTTGTAAGCAACTTGCTTCTAAATGCC
  CAGTTCACTTCTTTGCAGAGCCTTTTACCCCTGCACAGTTTAGAACAGAGGGACCAAATT
  GCTTCTAGGAGTCAACTGGCTGGCCAGTCTGGGTCTGGGTTTGGATCTCCAATTGCCTCT
  TGCAGGCTGAGTCCCTCCATGCAAAAGTGGGGCTAAATGAAGTGTGTTAAGGGGTCGGCT
  AAGTGGGACATTAGTAACTGCACACTATTTCCCTCTACTGAGTAAACCCTATCTGTGATT
  CCCCCAAACATCTGGCATGGCTCCCTTTTGTCCTTCCTGTGCCCTGCAAATATTAGCAAA
  GAAGCTTCATGCCAGGTTAGGAAGGCAGCATTCCATGACCAGAAACAGGGACAAAGAAAT
  CCCCCCTTCAGAACAGAGGCATTTAAAATGGAAAAGAGAGATTGGATTTTGGTGGGTAAC
  TTAGAAGGATGGCATCTCCATGTAGAATAAATGAAGAAAGGGAAGGCCCAGCCGCCAGGA
  AGGCAGAATAAATCCTTGGGAGTCATTACCACGCCTTGACCTTCCCAAGGTTACTCAGCA
  GCAGAGAGCCCTGGGTGACTTCAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000800 unedited
  TGTATACGACTCACTATAGGCGGCACGCGATTCGGCACGAGGGCCGCCACATTATCTCCA
  TTCCTCCAGTGCCCGCCTGACACTGGCCCTGATNAGGGCTGGAGGGGGCAGGCATTTCTC
  ATTTACTAAAGTGCTGGATGCAGCCCTTGAGGTTCGGCAGAAGCAGAAAGCTGCGTCTTG
  AAAGCGCCACAAGCAGCAGCTGCTGAGCCATGGCTGAAGGGGAAATCACCACCTTCACAG
  CCCTGACCGAGAAGTTTAATCTGCCTCCAGGGAATTACAAGAAGCCCAAACTCCTCTACT
  GTAGCAACGGGGGCCACTTCCTGAGGATCCTTCCGGATGGCACAGTGGATGGGACAAGGG
  ACAGGAGCGACCAGCACATTCAGCTGCAGCTCAGTGCGGAAAGCGTGGGGGAGGTGTATA
  TAAAGAGTACCGAGACTGGCCAGTACTTGGCCATGGACACCGACGGGCTTTTATACGGCT
  CACAGACACCAAATGAGGAATGTTTGTTCCTGGAAAGGCTGGAGGAGAACCATTACAACA
  CCTATATATCCAAGAAGCATGCAGAGAAGAATTGGTTTGNTTGGCCTCAAGAAGAATGGG
  AGCTGCAAACGCGGTCCTCGGACTCACTATGGCCAGAAAGCAATCTTGTTTTCTNCCCCT
  GCCAGTCTCTTCTGATTAAAGAGATCTGNTCTGGGGTGTTGACCACTCCAGAGAAAGTTC
  GAGGGGTCCTCACCTGGNTTGACCAAAAATGTTCCCTTGACCATTGGCTGGNCTAACCCC
  CAGCCNACAGAGCCTGATTNTGTAGCACTTGCTTCTAATGCCCAGTCACTTCTTGCAGAG
  CTTTTACCCTGCCAGTTAAACAGAGGACAAATGCTCTAGATCACTGCTGGCACCCGGNCT
  GGTTAGACCTCAATGCTCTGCGGTGATCCTCTGCAATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000800
• Insert Size: 4690 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000800.2, NP_000791.1
• RefSeq Size: 2357 bp
• RefSeq ORF: 468 bp
• Locus ID: 2246
• Cytogenetics: 5q31.3
• Domains: FGF
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton
• Gene Summary: 'The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]'
  Transcript Variant: This variant (1) encodes the full-length isoform (1). Variants 1, 4-7, 9-12, and 15-20 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.