PDGF beta (PDGFB) (NM_002608) Human Untagged Clone

CAT#: SC111665

PDGFB (untagged)-Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1


  "NM_002608" in other vectors (7)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PDGFB
Synonyms c-sis; IBGC5; PDGF-2; PDGF2; SIS; SSV
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC111665 sequence for NM_002608 edited (data generated by NextGen Sequencing)
ATGAATCGCTGCTGGGCGCTCTTCCTGTCTCTCTGCTGCTACCTGCGTCTGGTCAGCGCC
GAGGGGGACCCCATTCCCGAGGAGCTTTATGAGATGCTGAGTGACCACTCGATCCGCTCC
TTTGATGATCTCCAACGCCTGCTGCACGGAGACCCCGGAGAGGAAGATGGGGCCGAGTTG
GACCTGAACATGACCCGCTCCCACTCTGGAGGCGAGCTGGAGAGCTTGGCTCGTGGAAGA
AGGAGCCTGGGTTCCCTGACCATTGCTGAGCCGGCCATGATCGCCGAGTGCAAGACGCGC
ACCGAGGTGTTCGAGATCTCCCGGCGCCTCATAGACCGCACCAACGCCAACTTCCTGGTG
TGGCCGCCCTGTGTGGAGGTGCAGCGCTGCTCCGGCTGCTGCAACAACCGCAACGTGCAG
TGCCGCCCCACCCAGGTGCAGCTGCGACCTGTCCAGGTGAGAAAGATCGAGATTGTGCGG
AAGAAGCCAATCTTTAAGAAGGCCACGGTGACGCTGGAAGACCACCTGGCATGCAAGTGT
GAGACAGTGGCAGCTGCACGGCCTGTGACCCGAAGCCCGGGGGGTTCCCAGGAGCAGCGA
GCCAAAACGCCCCAAACTCGGGTGACCATTCGGACGGTGCGAGTCCGCCGGCCCCCCAAG
GGCAAGCACCGGAAATTCAAGCACACGCATGACAAGACGGCACTGAAGGAGACCCTTGGA
GCCTAG

Clone variation with respect to NM_002608.2
>OriGene 5' read for NM_002608 unedited
NGGGTAATACCCCCGCCCGTTGNCGCATAGGGCGGTAGGCGTGTACGGTGGGAGGTCTAT
ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG
GCCGCGAATTCGGCACGAGGGCGGACCCGAGCCGAGCCCACCCCCCTCCCCAGCCCCCCA
CCCTGGCCGCGGGGGCGGCGCGCTCGATCTACGCGTCCGGGGCCCCGCGGGGCCGGGCCC
GGAGTCGGCATGAATCGCTGCTGGGCGCTCTTCCTGTCTCTCTGCTGCTACCTGCGTCTG
GTCAGCGCCGAGGGGGACCCCATTCCCGAGGAGCTTTATGAGATGCTGAGTGACCACTCG
ATCCGCTCCTTTGATGATCTCCAACGCCTGCTGCACGGAGACCCCGGAGAGGAAGATGGG
GCCGAGTTGGACCTGAACATGACCCGCTCCCACTCTGGAGGCGAGCTGGAGAGCTTGGCT
CGTGGAAGAAGGAGCCTGGGTTCCCTGACCATTGCTGAGCCGGCCATGATCGCCGAGTGC
AAGACGCGCACCGAGGTGTTCGAGATCTCCCGGCGCCTCATAGACCGCACCAACGCCAAC
TTCCTGGTGTGGCCGCCCTGTGTGGAGGTGCAGCGCTGCTCCGGCTGCTGCAACAACCGC
AACGTGCAGTGCCGCCCCACCCAGGTGCAGCTGCGACCTGTCCAGGTGAGAAAGATCGAG
ATTGTGCGGAAGAAGCCAATCTTTAAGAAGGCCACGGTGACGCTGGAAGACCACCTGGNC
ATGCAGTGTGAGACAGTGGCAGCTGCACGGCCTGTGACCCGAGCCCGGNGGGGTCCCAGG
ACAGCGAGCCAAACGCCCCCAACTCGGTGACCATTCGACGGTGCGAGTCCGCCGGCCC
>OriGene 3' read for NM_002608 unedited
NGGGAATACTATGTACCCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTCCGGCGGATTCATTTGGTTTTGTTTTGGGGTTATTTTTTTTT
ACAACTTTAAATACGGAATATAAATAAATTTTACATTTAAAAAATAAAAGGAAAGCCCCC
AAAAATATAATCACCGACTTTACAAACTGAAGGAAGCAGGTTTTTTAAAGCGGGAAGGGG
GAAAGTGCAGTAGGTGGGAAGGGAGGTGGGAGCTCAAATCCCACCCCAGGGGGTCTTGGG
GATCTTTTCCCTTTTTTCCCCCATTCCCAGCCCACGAGCTGGTTTCCTAGGAGGAGCCAC
CAGAGGTGGAGCTCAAAAGGATCAAAGATGGGGAAGTGTGCAAGCAGGGAGGGGTGCGGG
CAAAAAGGCTGCACCCTCCCTTGGAGACCTTCTCCCAGGTGTCCCACACCCACCTGGAAG
GGCAGTTGCTGGAGCAAAGGACTTTGGGAAATGGAGGTCATGTGGACAGCAGTGTTGCCT
CCCTGCCCCTACCACAGTCTCCCTCCTATTTGGCCACTCCAAGCCACAGGCTGGATCAGG
GGCACAGCCCGTCCACAAGGAGTCTTTGTGTGAATTAAGAAAAGGATCCTTTTCCTCTTT
ACCTACATCTGCCAGAAAATGGCATAATAAATATACAAATCAGCTTTATTTATGATGTGG
ACAGTGCTCCCTGGATACAAAGTGCTTGGGGAGTACACACCCTGGACAAACTTACATGGG
AATCGTGGGTGGGTTGGAATATAAAGAGGAAGTCCCTTGCTGGCCTGGCCTCTTATTTTT
TGCCCTAAAAAGGAAGGCTGGGATCCATTGGGGGCCTTAAAAAGGGGTCAAG
Restriction Sites NotI-NotI     
ACCN NM_002608
Insert Size 2800 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002608.1, NP_002599.1
RefSeq Size 3373 bp
RefSeq ORF 726 bp
Locus ID 5155
Cytogenetics 22q13.1
Domains PDGF, PDGF_N
Protein Families Druggable Genome
Protein Pathways Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma
Gene Summary 'This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1), which contains a signal peptide.

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