PDGF beta (PDGFB) (NM_002608) Human Untagged Clone

CAT#: SC111665

PDGFB (untagged)-Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDGFB
• Synonyms: c-sis; IBGC5; PDGF-2; PDGF2; SIS; SSV
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111665 sequence for NM_002608 edited (data generated by NextGen Sequencing)
  ATGAATCGCTGCTGGGCGCTCTTCCTGTCTCTCTGCTGCTACCTGCGTCTGGTCAGCGCC
  GAGGGGGACCCCATTCCCGAGGAGCTTTATGAGATGCTGAGTGACCACTCGATCCGCTCC
  TTTGATGATCTCCAACGCCTGCTGCACGGAGACCCCGGAGAGGAAGATGGGGCCGAGTTG
  GACCTGAACATGACCCGCTCCCACTCTGGAGGCGAGCTGGAGAGCTTGGCTCGTGGAAGA
  AGGAGCCTGGGTTCCCTGACCATTGCTGAGCCGGCCATGATCGCCGAGTGCAAGACGCGC
  ACCGAGGTGTTCGAGATCTCCCGGCGCCTCATAGACCGCACCAACGCCAACTTCCTGGTG
  TGGCCGCCCTGTGTGGAGGTGCAGCGCTGCTCCGGCTGCTGCAACAACCGCAACGTGCAG
  TGCCGCCCCACCCAGGTGCAGCTGCGACCTGTCCAGGTGAGAAAGATCGAGATTGTGCGG
  AAGAAGCCAATCTTTAAGAAGGCCACGGTGACGCTGGAAGACCACCTGGCATGCAAGTGT
  GAGACAGTGGCAGCTGCACGGCCTGTGACCCGAAGCCCGGGGGGTTCCCAGGAGCAGCGA
  GCCAAAACGCCCCAAACTCGGGTGACCATTCGGACGGTGCGAGTCCGCCGGCCCCCCAAG
  GGCAAGCACCGGAAATTCAAGCACACGCATGACAAGACGGCACTGAAGGAGACCCTTGGA
  GCCTAG
  
  Clone variation with respect to NM_002608.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002608 unedited
  NGGGTAATACCCCCGCCCGTTGNCGCATAGGGCGGTAGGCGTGTACGGTGGGAGGTCTAT
  ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG
  GCCGCGAATTCGGCACGAGGGCGGACCCGAGCCGAGCCCACCCCCCTCCCCAGCCCCCCA
  CCCTGGCCGCGGGGGCGGCGCGCTCGATCTACGCGTCCGGGGCCCCGCGGGGCCGGGCCC
  GGAGTCGGCATGAATCGCTGCTGGGCGCTCTTCCTGTCTCTCTGCTGCTACCTGCGTCTG
  GTCAGCGCCGAGGGGGACCCCATTCCCGAGGAGCTTTATGAGATGCTGAGTGACCACTCG
  ATCCGCTCCTTTGATGATCTCCAACGCCTGCTGCACGGAGACCCCGGAGAGGAAGATGGG
  GCCGAGTTGGACCTGAACATGACCCGCTCCCACTCTGGAGGCGAGCTGGAGAGCTTGGCT
  CGTGGAAGAAGGAGCCTGGGTTCCCTGACCATTGCTGAGCCGGCCATGATCGCCGAGTGC
  AAGACGCGCACCGAGGTGTTCGAGATCTCCCGGCGCCTCATAGACCGCACCAACGCCAAC
  TTCCTGGTGTGGCCGCCCTGTGTGGAGGTGCAGCGCTGCTCCGGCTGCTGCAACAACCGC
  AACGTGCAGTGCCGCCCCACCCAGGTGCAGCTGCGACCTGTCCAGGTGAGAAAGATCGAG
  ATTGTGCGGAAGAAGCCAATCTTTAAGAAGGCCACGGTGACGCTGGAAGACCACCTGGNC
  ATGCAGTGTGAGACAGTGGCAGCTGCACGGCCTGTGACCCGAGCCCGGNGGGGTCCCAGG
  ACAGCGAGCCAAACGCCCCCAACTCGGTGACCATTCGACGGTGCGAGTCCGCCGGCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002608 unedited
  NGGGAATACTATGTACCCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTCCGGCGGATTCATTTGGTTTTGTTTTGGGGTTATTTTTTTTT
  ACAACTTTAAATACGGAATATAAATAAATTTTACATTTAAAAAATAAAAGGAAAGCCCCC
  AAAAATATAATCACCGACTTTACAAACTGAAGGAAGCAGGTTTTTTAAAGCGGGAAGGGG
  GAAAGTGCAGTAGGTGGGAAGGGAGGTGGGAGCTCAAATCCCACCCCAGGGGGTCTTGGG
  GATCTTTTCCCTTTTTTCCCCCATTCCCAGCCCACGAGCTGGTTTCCTAGGAGGAGCCAC
  CAGAGGTGGAGCTCAAAAGGATCAAAGATGGGGAAGTGTGCAAGCAGGGAGGGGTGCGGG
  CAAAAAGGCTGCACCCTCCCTTGGAGACCTTCTCCCAGGTGTCCCACACCCACCTGGAAG
  GGCAGTTGCTGGAGCAAAGGACTTTGGGAAATGGAGGTCATGTGGACAGCAGTGTTGCCT
  CCCTGCCCCTACCACAGTCTCCCTCCTATTTGGCCACTCCAAGCCACAGGCTGGATCAGG
  GGCACAGCCCGTCCACAAGGAGTCTTTGTGTGAATTAAGAAAAGGATCCTTTTCCTCTTT
  ACCTACATCTGCCAGAAAATGGCATAATAAATATACAAATCAGCTTTATTTATGATGTGG
  ACAGTGCTCCCTGGATACAAAGTGCTTGGGGAGTACACACCCTGGACAAACTTACATGGG
  AATCGTGGGTGGGTTGGAATATAAAGAGGAAGTCCCTTGCTGGCCTGGCCTCTTATTTTT
  TGCCCTAAAAAGGAAGGCTGGGATCCATTGGGGGCCTTAAAAAGGGGTCAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002608
• Insert Size: 2800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002608.1, NP_002599.1
• RefSeq Size: 3373 bp
• RefSeq ORF: 726 bp
• Locus ID: 5155
• Cytogenetics: 22q13.1
• Domains: PDGF, PDGF_N
• Protein Families: Druggable Genome
• Protein Pathways: Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma
• Gene Summary: 'This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1), which contains a signal peptide.