PDGF beta (PDGFB) (NM_002608) Human Untagged Clone

CAT#: SC324287

PDGFB (untagged)-Human platelet-derived growth factor beta polypeptide (PDGFB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDGFB
• Synonyms: c-sis; IBGC5; PDGF-2; PDGF2; SIS; SSV
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002608.1 GACGCCTCGGCTGGTTCGCGACGCAGCCCCCCGGCCGTGGATGCTCACTCGGGCTCGGGA
  TCCGCCCAGGTAGCGGCCTCGGACCCAGGTCCTGCGCCCAGGTCCTCCCCTGCCCCCCAG
  CGACGGAGCCGGGGCCGGGGGCGGCGGCGCCCGGGGGCCATGCGGGTGAGCCGCGGCTGC
  AGAGGCCTGAGCGCCTGATCGCCGCGGACCCGAGCCGAGCCCACCCCCCTCCCCAGCCCC
  CCACCCTGGCCGCGGGGGCGGCGCGCTCGATCTACGCGTCCGGGGCCCCGCGGGGCCGGG
  CCCGGAGTCGGCATGAATCGCTGCTGGGCGCTCTTCCTGTCTCTCTGCTGCTACCTGCGT
  CTGGTCAGCGCCGAGGGGGACCCCATTCCCGAGGAGCTTTATGAGATGCTGAGTGACCAC
  TCGATCCGCTCCTTTGATGATCTCCAACGCCTGCTGCACGGAGACCCCGGAGAGGAAGAT
  GGGGCCGAGTTGGACCTGAACATGACCCGCTCCCACTCTGGAGGCGAGCTGGAGAGCTTG
  GCTCGTGGAAGAAGGAGCCTGGGTTCCCTGACCATTGCTGAGCCGGCCATGATCGCCGAG
  TGCAAGACGCGCACCGAGGTGTTCGAGATCTCCCGGCGCCTCATAGACCGCACCAACGCC
  AACTTCCTGGTGTGGCCGCCCTGTGTGGAGGTGCAGCGCTGCTCCGGCTGCTGCAACAAC
  CGCAACGTGCAGTGCCGCCCCACCCAGGTGCAGCTGCGACCTGTCCAGGTGAGAAAGATC
  GAGATTGTGCGGAAGAAGCCAATCTTTAAGAAGGCCACGGTGACGCTGGAAGACCACCTG
  GCATGCAAGTGTGAGACAGTGGCAGCTGCACGGCCTGTGACCCGAAGCCCGGGGGGTTCC
  CAGGAGCAGCGAGCCAAAACGCCCCAAACTCGGGTGACCATTCGGACGGTGCGAGTCCGC
  CGGCCCCCCAAGGGCAAGCACCGGAAATTCAAGCACACGCATGACAAGACGGCACTGAAG
  GAGACCCTTGGAGCCTAGGGGCATCGGCAGGAGAGTGTGTGGGCAGGGTTATTTAATATG
  GTATTTGCTGTATTGCCCCCATGGGGTCCTTGGAGTGATAATATTGTTTCCCTCGTCCGT
  CTGTCTCGATGCCTGATTCGGACGGCCAATGGTGCTTCCCCCACCCCTCCACGTGTCCGT
  CCACCCTTCCATCAGCGGGTCTCCTCCCAGCGGCCTCCGGCGTCTTGCCCAGCAGCTCAA
  GAAGAAAAAGAAGGACTGAACTCCATCGCCATCTTCTTCCCTTAACTCCAAGAACTTGGG
  ATAAGAGTGTGAGAGAGACTGATGGGGTCGCTCTTTGGGGGAAACGGGCTCCTTCCCCTG
  CACCTGGCCTGGGCCACACCTGAGCGCTGTGGACTGTCCTGAGGAGCCCTGAGGACCTCT
  CAGCATAGCCTGCCTGATCCCTGAACCCCTGGCCAGCTCTGAGGGGAGGCACCTCCAGGC
  AGGCCAGGCTGCCTCGGACTCCATGGCTAAGACCACAGACGGGCACACAGACTGGAGAAA
  ACCCCTCCCACGGTGCCCAAACACCAGTCACCTCGTCTCCCTGGTGCCTCTGTGCACAGT
  GGCTTCTTTTCGTTTTCGTTTTGAAGACGTGGACTCCTCTTGGTGGGTGTGGCCAGCACA
  CCAAGTGGCTGGGTGCCCTCTCAGGTGGGTTAGAGATGGAGTTTGCTGTTGAGGTGGCTG
  TAGATGGTGACCTGGGTATCCCCTGCCTCCTGCCACCCCTTCCTCCCCACACTCCACTCT
  GATTCACCTCTTCCTCTGGTTCCTTTCATCTCTCTACCTCCACCCTGCATTTTCCTCTTG
  TCCTGGCCCTTCAGTCTGCTCCACCAAGGGGCTCTTGAACCCCTTATTAAGGCCCCAGAT
  GATCCCAGTCACTCCTCTCTAGGGCAGAAGACTAGAGGCCAGGGCAGCAAGGGACCTGCT
  CATCATATTCCAACCCAGCCACGACTGCCATGTAAGGTTGTGCAGGGTGTGTACTGCACA
  AGGACATTGTATGCAGGGAGCACTGTTCACATCATAGATAAAGCTGATTTGTATATTTAT
  TATGACAATTTCTGGCAGATGTAGGTAAAGAGGAAAAGGATCCTTTTCCTAATTCACACA
  AAGACTCCTTGTGGACTGGCTGTGCCCCTGATGCAGCCTGTGGCTTGGAGTGGCCAAATA
  GGAGGGAGACTGTGGTAGGGGCAGGGAGGCAACACTGCTGTCCACATGACCTCCATTTCC
  CAAAGTCCTCTGCTCCAGCAACTGCCCTTCCAGGTGGGTGTGGGACACCTGGGAGAAGGT
  CTCCAAGGGAGGGTGCAGCCCTCTTGCCCGCACCCCTCCCTGCTTGCACACTTCCCCATC
  TTTGATCCTTCTGAGCTCCACCTCTGGTGGCTCCTCCTAGGAAACCAGCTCGTGGGCTGG
  GAATGGGGGAGAGAAGGGAAAAGATCCCCAAGACCCCCTGGGGTGGGATCTGAGCTCCCA
  CCTCCCTTCCCACCTACTGCACTTTCCCCCTTCCCGCCTTCCAAAACCTGCTTCCTTCAG
  TTTGTAAAGTCGGTGATTATATTTTTGGGGGCTTTCCTTTTATTTTTTAAATGTAAAATT
  TATTTATATTCCGTATTTAAAGTTGTAAAAAAAAATAACCACAAAACAAAACCAAAAAAA
  AAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002608
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002608.1, NP_002599.1
• RefSeq Size: 3373 bp
• RefSeq ORF: 726 bp
• Locus ID: 5155
• Cytogenetics: 22q13.1
• Domains: PDGF, PDGF_N
• Protein Families: Druggable Genome
• Protein Pathways: Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma
• Gene Summary: 'This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1), which contains a signal peptide.