SNAP25 (NM_003081) Human Untagged Clone

CAT#: SC111689

SNAP25 (untagged)-Human synaptosomal-associated protein, 25kDa (SNAP25), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SNAP25
• Synonyms: bA416N4.2; CMS18; dJ1068F16.2; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SUP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003081 edited
  GAATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGGGGAGGTCAGGCGCTGTCTTTC
  CTTCCCTCCCTGCTCGGCGGCTCCACCACAGTTGCAACCTGCAGAGGCCCGGAGAACACA
  ACCCTCCCGAGAAGCCCAGGTCCAGAGCCAAACCCGTCACTGACCCCCCAGCCCAGGCGC
  CCAGCCACTCCCCACCGCTACCATGGCCGAAGACGCAGACATGCGCAATGAGCTGGAGGA
  GATGCAGCGAAGGGCTGACCAGTTGGCTGATGAGTCGCTGGAAAGCACCCGTCGTATGCT
  GCAACTGGTTGAAGAGAGTAAAGATGCTGGTATCAGGACTTTGGTTATGTTGGATGAACA
  AGGAGAACAACTGGAACGCATTGAGGAAGGGATGGACCAAATCAATAAGGACATGAAAGA
  AGCAGAAAAGAATTTGACGGACCTAGGAAAATTCTGCGGGCTTTGTGTGTGTCCCTGTAA
  CAAGCTTAAATCAAGTGATGCTTACAAAAAAGCCTGGGGCAATAATCAGGACGGAGTGGT
  GGCCAGCCAGCCTGCTCGTGTAGTGGACGAACGGGAGCAGATGGCCATCAGTGGCGGCTT
  CATCCGCAGGGTAACAAATGATGCCCGAGAAAATGAAATGGATGAAAACCTAGAGCAGGT
  GAGCGGCATCATCGGGAACCTCCGTCACATGGCCCTGGATATGGGCAATGAGATCGATAC
  ACAGAATCGCCAGATCGACAGGATCATGGAGAAGGCTGATTCCAACAAAACCAGAATTGA
  TGAGGCCAACCAACGTGCAACAAAGATGCTGGGAAGTGGTTAAGTGTGCCCACCCGTGTT
  CTCCTCCAAATGCTGTCGGGCAAGATAGCTCCTTCATGCTTTTCTCATGGTATTATCTAG
  TAGGTCTGCACACATAACACACATCAGTCCACCCCCATTGTGAATGTTGTCCTGTGTCAT
  CTGTCAGCTTCCCAACAATACTTTGTGTCTTTTGTTCTCTCTTGGTCTCTTTCTTTCCAA
  AGGTTGTACATAGTGGTCATTTGGTGGCTCTAACTCCTTGATGTCCTGAGTTTCATTTTT
  CATTTTCTCTCCTCGGTGGCATTTGCTGAATAACAACAATTTAGGAATGCTCAATGTGCT
  GTTGATTCTTTCAATCCACAGTATTGTTCTTGTAAAACTGTGACATTCCACAGAGTTACT
  GCCACGGTCCTTTGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTTCCTC
  ATGGCTGTTATCTGTCTTTATGATTTCATGATTAGACAATGTGGAATTATATAACAGGCA
  TTGCACTAAAAGTGATGTGATTTATGCATTTATGCATGAGAACTAAATAGATTTTTAGAT
  TCCTACTTAAACAAAAACTTTCCATGACAGTAGCATACTGATGAGACAACACACACACAC
  ACAAAACAACAGCAACAACAACAGAACAACAACAAAGCATGCTCAGTATTGAGACACTGT
  CAAGATTAAGTTATACCAGCAAAAGTGCAGTAGTGTCACTTTTTTTCCTGTCAATATATA
  GAGACTTCTAAATCATAATCATCCTTTTTTAAAAAAAAGAATTTTAAAAAAGATGGATTT
  GACACACTCACCATTTAATCATTTCCAGCAAAATATATGTTTGGCTGAAATTATGTCAAA
  TGGATGTAATATAGGGTTTGTTTGCTGCTTTTGATGGCTACGTTTTGGAGAGAGCAATCT
  TGCTGTGAAACAGTGTGGATGTAAATTTTATAAGGCTGACTCTTACTAACCACCATTTCC
  CCTGTGGTTTGTTATCAGTACAATTCTTTGTTGCTTAATCTAGAGCTATGCACACCAAAT
  TGCTGAGATGTTTAGTAGCTGATAAAGAAACCTTTTAAAAAAATAATATAAATGAATGAA
  ATATAAACTGTGAGATAAATATCATTATAGCATGTAATATTAAATTCCTCCTGTCTCCTC
  TGTCAGTTTGTGAAGTGATTGACATTTTGTAGCTAGTTTAAAATTATTAAAAATTATAGA
  CTCCAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003081 unedited
  GGTTAATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCCTCGTGCCG
  AATTCGGCACGAGGGGAGGTCAGGCGCTGTCTTTCCTTCCCTCCCTGCTCGGCGGCTCCA
  CCACAGTTGCAACCTGCAGAGGCCCGGAGAACACAACCCTCCCGAGAAGCCCAGGTCCAG
  AGCCAAACCCGTCACTGACCCCCCAGCCCAGGCGCCCAGCCACTCCCCACCGCTACCATG
  GCCGAAGACGCAGACATGCGCAATGAGCTGGAGGAGATGCAGCGAAGGGCTGACCAGTTG
  GCTGATGAGTCGCTGGAAAGCACCCGTCGTATGCTGCAACTGGTTGAAGAGAGTAAAGAT
  GCTGGTATCAGGACTTTGGTTATGTTGGATGAACAAGGAGAACAACTGGAACGCATTGAG
  GAAGGGATGGACCAAATCAATAAGGACATGAAAGAAGCAGAAAAGAATTTGACGGACCTA
  GGAAAATTCTGCGGGCTTNTGTGTGTGTCCCTGTAACAAGCTTAAATCAAGTGATGCTTA
  CAAAAAAGCCTGGGGCAATAATCAGGAACGGAGTGGTGGCCAGCCAGCCTGGCTCGTGTA
  GTGGACGAACGGGAGCAGATGGCCATCAGTGGCGGCTTCATCCGCAGGGTAACAAATGAT
  GCCCGAGAAATGAAATGGATGAAACCTANAGCANGTGAGCGGCATATCGGGAACCCTNCG
  TACATGGCCCTGGATATGGGCATGGANATCGATCACAGAATCGCCAGATGAACAGGATCA
  TGGGGAAGCTTGATTCAACAAACCGAATGATGAGGCAACCCACGGCACAAATGCTGGGAT
  GGGTTAATGTGGCCCCCCCGGTTTTCTCCAAGAGGTGCGGCAGAAAACTCTTCTGCTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003081 unedited
  TGGCAACTTCCAGGGCCGGNAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATC
  TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT
  TTGGAGTCTATAATTTTTAATAATTTTAAACTAGCTACAAAATGTCAATCACTTCACAAA
  CTGACAGAGGAGACAGGAGGAATTTAATATTACATGCTATAATGATATTTATCTCACAGT
  TTATATTTCATTCATTTATATTATTTTTTTAAAAGGTTTCTTTATCAGCTACTAAACATC
  TCAGCAATTTGGTGTGCATAGCTCTAGATTAAGCAACAAAGAATTGTACTGATAACAAAC
  CACAGGGGAAATGGTGGTTAGTAAGAGTCAGCCTTATAAAATTTACATCCACACTGTTTC
  ACAGCAAGATTGCTCTCTCCAAAACGTAGCCATCAAAAGCAGCAAACAAACCCTATATTA
  CATCCATTTGACATAATTTCAGCCAAACATATATTTTGCTGGAAATGATTAAATGGTGAN
  GTGTGTCAAATCCATCTTTTTTAAAATTCTTTTTTTTAAAAAAGGATGATTATNGATTTA
  AAAGTCTCTATATATTGACAGGAAAAAAAGTGACACTACTGCACTTTTGCTGGTATAACT
  TAATCTTGACAGTGTCTCATACTGAGCTGCTTTGNNNTGTGTCTGNNNTGTGTGCCTGTG
  GTTTTGGGGGGGNGNGGGTTGCTCATCAGATGCTACTGCATGGAAAAGTTTTGGTTAAGT
  AGGATCTAAAATCTATTTAGTTCTCATGCTAATGCATATCACATCCTTTTAGTGCAAGGC
  TGTATATAATTCCCATGNCTAATCTGAATCTAAGACGATAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003081
• Insert Size: 2040 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003081.2, NP_003072.2
• RefSeq Size: 2053 bp
• RefSeq ORF: 621 bp
• Locus ID: 6616
• Cytogenetics: 20p12.2
• Domains: t_SNARE, SNAP-25
• Protein Families: Druggable Genome
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: 'Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) contains 8 exons and encodes isoform SNAP25A.