SNAP25 (NM_003081) Human Untagged Clone

CAT#: SC322262

SNAP25 (untagged)-Human synaptosomal-associated protein, 25kDa (SNAP25), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SNAP25
• Synonyms: bA416N4.2; CMS18; dJ1068F16.2; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SUP
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322262 TGCTCGGCGGCTCCACCACAGTTGCAACCTGCAGAGGCCCGGAGAACACAACCCTCCCGA
  GAAGCCCAGGTCCAGAGCCAAACCCGTCACTGACCCCCCAGCCCAGGCGCCCAGCCACTC
  CCCACCGCTACCATGGCCGAAGACGCAGACATGCGCAATGAGCTGGAGGAGATGCAGCGA
  AGGGCTGACCAGTTGGCTGATGAGTCGCTGGAAAGCACCCGTCGTATGCTGCAACTGGTT
  GAAGAGAGTAAAGATGCTGGTATCAGGACTTTGGTTATGTTGGATGAACAAGGAGAACAA
  CTCGATCGTGTCGAAGAAGGCATGAACCATATCAACCAAGACATGAAGGAGGCTGAGAAA
  AATTTAAAAGATTTAGGGAAATGCTGTGGCCTTTTCATATGTCCTTGTAACAAGCTTAAA
  TCAAGTGATGCTTACAAAAAAGCCTGGGGCAATAATCAGGACGGAGTGGTGGCCAGCCAG
  CCTGCTCGTGTAGTGGACGAACGGGAGCAGATGGCCATCAGTGGCGGCTTCATCCGCAGG
  GTAACAAATGATGCCCGAGAAAATGAAATGGATGAAAACCTAGAGCAGGTGAGCGGCATC
  ATCGGGAACCTCCGTCACATGGCCCTGGATATGGGCAATGAGATCGATACACAGAATCGC
  CAGATCGACAGGATCATGGAGAAGGCTGATTCCAACAAAACCAGAATTGATGAGGCCAAC
  CAACGTGCAACAAAGATGCTGGGAAGTGGTTAAGTGTGCCCACCCGTGTTCTCCTCCAAA
  TGCTGTCGGGCAAGATAGCTCCTTCATGCTTTTCTCATGGTATTATCTAGTAGGTCTGCA
  CACATAACACACATCAGTCCACCCCCATTGTGAATGTTGTCCTGTGTCATCTGTCAGCTT
  CCCAACAATACTTTGTGTCTTTTGTTCTCTCTTGGTCTCTTTCTTTCCAAAGGTTGTACA
  TAGTGGTCATTTGGTGGCTCTAACTCCTTGATGTCCTGAGTTTCATTTTTCATTTTCTCT
  CCTCGGTGGCATTTGCTGAATAACAACAATTTAGGAATGCTCAATGTGCTGTTGATTCTT
  TCAATCCACAGTATTGTTCTTGTAAAACTGTGACATTCCACAGAGTTACTGCCACGGTCC
  TTTGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTTCCTCATGGCTGTTA
  TCTGTCTTTATGATTTCATGATTAGACAATGTGGAATTACATAACAGGCATTGCACTAAA
  AGTGATGTGATTTATGCATTTATGCATGAGAACTAAATAGATTTTTAGATTCCTACTTAA
  ACAAAAACTTTCCATGACAGTAGCATACTGATGAGACAACACACACACACACAAAACAAC
  AGCAACAACAACAGAACAACAACAAAGCATGCTCAGTATTGAGACACTGTCAAGATTAAG
  TTATACCAGCAAAAGTGCAGTAGTGTCACTTTTTTCCTGTCAATATATAGAGACTTCTAA
  ATCATAATCATCCTTTTTTAAAAAAAAGAATTTTAAAAAAGATGGATTTGACACACTCAC
  CATTTAATCATTTCCAGCAAAATATATGTTTGGCTGAAATTATGTCAAATGGATGTAATA
  TAGGGTTTGTTTGCTGCTTTTGATGGCTACGTTTTGGAGAGAGCAATCTTGCTGTGAAAC
  AGTGTGGATGTAAATTTTATAAGGCTGACTCTTACTAACCACCATTTCCCCTGTGGTTTG
  TTATCAGTACAATTCTTTGTTGCTTAATCTAGAGCTATGCACACCAAATTGCTGAGATGT
  TTAGTAGCTGATAAAGAAACCTTTTAAAAAAATAATATAAATGAATGAAATATAAACTGT
  GAGATAAATATCATTATAGCATGTAATATTAAATTCCTCCTGTCTCCTCTGTCAGTTTGT
  GAAGTGATTGACATTTTGTAGCTAGTTTAAAATTATTAAAAATTATAGACTCCAAAAAAA
  AAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003081
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003081.2, NP_003072.2
• RefSeq Size: 2053 bp
• RefSeq ORF: 621 bp
• Locus ID: 6616
• Cytogenetics: 20p12.2
• Domains: t_SNARE, SNAP-25
• Protein Families: Druggable Genome
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: 'Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) contains 8 exons and encodes isoform SNAP25A.