VIP (NM_003381) Human Untagged Clone

CAT#: SC111704

VIP (untagged)-Human vasoactive intestinal peptide (VIP), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VIP
• Synonyms: PHM27
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003381, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACACCAGAAATAAGGCCCAGCTCCTTGTGCTCCTGACTCTTCTCAGTGTGCTCTTCTCACAGACTT
  CGGCATGGCCTCTTTACAGGGCACCTTCTGCTCTCAGGTTGGGTGACAGAATACCCTTTGAGGGAGCAAA
  TGAACCTGATCAAGTTTCATTAAAAGAAGACATTGACATGTTGCAAAATGCATTAGCTGAAAATGACACA
  CCCTATTATGATGTATCCAGAAATGCCAGGCATGCTGATGGAGTTTTCACCAGTGACTTCAGTAAACTCT
  TGGGTCAACTTTCTGCCAAAAAGTACCTTGAGTCTCTTATGGGAAAACGTGTTAGCAGTAACATCTCAGA
  AGACCCTGTACCAGTCAAACGTCACTCAGATGCAGTCTTCACTGACAACTATACCCGCCTTAGAAAACAA
  ATGGCTGTAAAGAAATATTTGAACTCAATTCTGAATGGAAAGAGGAGCAGTGAGGGAGAATCTCCCGACT
  TTCCAGAAGAGTTAGAAAAATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003381 unedited
  CGGTCGCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGCAGGCAGT
  AACAGCCAACCCTTAGCCATTGCTAAGGGCAGAGAACTGGTGGAGCCTTTCTCTTACTCC
  CAGGACTTCAGCACCTAAGACAGCTCCAAAACAAACCAGAACAGTCAGCTCCGGGGGAGC
  ACGACTGGGCGAGAGGCACAGAAATGGACACCAGAAATAAGGCCCAGCTCCTTGTGCTCC
  TGACTCTTCTCAGTGTGCTCTTCTCACAGACTTCGGCATGGCCTCTTTACAGGGCACCTT
  CTGCTCTCAGGTTGGGTGACAGAATACCCTTTGAGGGAGCAAATGAACCTGATCAAGTTT
  CATTAAAAGAAGACATTGACATGTTGCAAAATGCATTAGCTGAAAATGACACACCCTATT
  ATGATGTATCCAGAAATGCCAGGCATGCTGATGGAGTTTTCACCAGTGACTTCAGTAAAC
  TCTTGGGTCAACTTTCTGCCAAAAAGTACCTTGAGTCTCTTATGGGAAAACGTGTTAGCA
  GTAACATCTCAAAAGACCCTGTACCAGTCAAACGTCACTCAGATGCAGTCTTCACTGACA
  ACTATACCCGCCTTAGAAAACAAATGGCTGTAAAGAAATATTTGAACTCAATTCTGAATG
  GAAAGAGGAGCAGTGAGGGAGAATCTCCCGACTTTCCAGAAGAGTTAGAAAAATGATGAA
  NAAGACCTTTGGAGCANAGCTGATGACCACTTCCCAGTGAATTCTTGAAGGAAACGATAC
  GCAACATAATTAAATTNTGAGTTCTACATAAGTAATTCAAGAAAACAACTTNCATATCCA
  ACCAATAAAAATATTGNNGTGNGAAATGGTGNGATGTATTCTAGCTAATGTANTAACTGG
  TGAAGTTCACATTGNNAATAGTATTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003381
• Insert Size: 1730 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003381.2, NP_003372.1
• RefSeq Size: 1618 bp
• RefSeq ORF: 513 bp
• Locus ID: 7432
• Cytogenetics: 6q25.2
• Domains: GLUCA
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: 'The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Nov 2014]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).