PKC gamma (PRKCG) (NM_002739) Human Untagged Clone

CAT#: SC112717

PRKCG (untagged)-Human protein kinase C, gamma (PRKCG)


  "NM_002739" in other vectors (7)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PRKCG
Synonyms PKC-gamma; PKCC; PKCG; PKCgamma; PKCI(3); SCA14
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_002739 edited
ATGGCTGGTCTGGGCCCCGGCGTAGGCGATTCAGAGGGGGGACCCCGGCCCCTGTTTTGC
AGAAAGGGGGCTCTGAGGCAGAAGGTGGTCCACGAAGTCAAGAGCCACAAGTTCACCGCT
CGCTTCTTCAAGCAGCCCACCTTCTGCAGCCACTGCACCGACTTCATCTGGGGTATCGGA
AAGCAGGGCCTGCAATGTCAAGTCTGCAGCTTTGTGGTTCATCGACGATGCCACGAATTT
GTGACCTTCGAGTGTCCAGGCGCTGGGAAGGGCCCCCAGACGGACGACCCCCGGAACAAA
CACAAGTTCCGCCTGCATAGCTACAGCAGCCCCACCTTCTGCGACCACTGTGGCTCCCTC
CTCTACGGGCTTGTGCACCAGGGCATGAAATGCTCCTGCTGCGAGATGAACGTGCACCGG
CGCTGTGTGCGTAGCGTGCCCTCCCTGTGCGGTGTGGACCACACCGAGCGCCGCGGGCGC
CTGCAGCTGGAGATCCGGGCTCCCACAGCAGATGAGATCCACGTAACTGTTGGCGAGGCC
CGTAACCTAATTCCTATGGACCCCAACGGTCTCTCTGATCCCTATGTGAAACTGAAGCTC
ATCCCAGACCCTCGGAACCTGACGAAACAGAAGACCCGAACGGTGAAAGCCACGCTAAAC
CCTGTGTGGAATGAGACCTTTGTGTTCAACCTGAAGCCAGGGGATGTGGAGCGCCGGCTC
AGCGTGGAGGTGTGGGACTGGGACCGGACCTCCCGCAACGACTTCATGGGGGCCATGTCC
TTTGGCGTCTCGGAGCTGCTCAAGGCGCCCGTGGATGGCTGGTACAAGTTACTGAACCAG
GAGGAGGGCGAGTATTACAATGTGCCGGTGGCCGATGCTGACAACTGCAGCCTCCTCCAG
AAGTTTGAGGCTTGTAACTACCCCCTGGAATTGTATGAGCGGGTGCGGATGGGCCCCTCT
TCCTCTCCCATCCCCTCCCCTTCCCCTAGTCCCACCGACCCCAAGCGCTGCTTCTTCGGG
GCGAGTCCAGGACGCCTGCACATCTCCGACTTCAGCTTCCTCATGGTTCTAGGAAAAGGC
AGTTTTGGGAAGGTGATGCTGGCCGAGCGCAGGGGCTCTGATGAGCTCTACGCCATCAAG
ATCTTGAAAAAGGACGTGATCGTCCAGGACGACGATGTGGACTGCACGCTGGTGGAGAAA
CGTGTGCTGGCGCTGGGGGGCCGGGGTCCTGGCGGCCGGCCCCACTTCCTCACCCAGCTC
CACTCCACCTTCCAGACCCCGGACCGCCTGTATTTCGTGATGGAGTACGTCACCGGGGGA
GACTTGATGTACCACATTCAACAGCTGGGCAAGTTTAAGGAGCCCCATGCAGCGTTCTAC
GCGGCAGAAATCGCTATCGGCCTCTTCTTCCTTCACAATCAGGGCATCATCTACAGGGAC
CTGAAGCTGGACAATGTGATGCTGGATGCTGAGGGACACATCAAGATCACTGACTTTGGC
ATGTGTAAGGAGAACGTCTTCCCCGGGACGACAACCCGCACCTTCTGCGGGACCCCGGAC
TACATAGCCCCGGAGATCATTGCCTACCAGCCCTATGGGAAGTCTGTCGATTGGTGGTCC
TTTGGAGTTCTGCTGTATGAGATGTTGGCAGGACAGCCTCCCTTCGATGGGGAGGACGAG
GAGGAGCTGTTTCAGGCCATCATGGAACAAACTGTCACCTACCCCAAGTCGCTTTCCCGG
GAAGCCGTGGCCATCTGCAAGGGGTTCCTGACCAAGCACCCAGGGAAGCGCCTGGGCTCA
GGGCCTGATGGGGAACCTACCATCCGTGCACATGGCTTTTTCCGCTGGATTGACTGGGAG
CGGCTGGAACGATTGGAGATCCCGCCTCCTTTCAGACCCCGCCCGTGTGGCCGCAGCGGC
GAGAACTTTGACAAGTTCTTCACGCGGGCGGCGCCAGCGCTGACCCCTCCAGACCGCCTA
GTCCTGGCCAGCATCGACCAGGCCGATTTCCAGGGCTTCACCTACGTGAACCCCGACTTC
GTGCACCCGGATGCCCGCAGCCCCACCAGCCCAGTGCCTGTGCCCGTCATGTAA
>OriGene 5' read for NM_002739 unedited
CCCCCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTATATAAGCAG
AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA
TTCGGCACGAGGCCCTGGCGGAGCCGGCGCGCCCGGGGTGCCGCTCCCTGCCTGGCGCGC
TCCGCACCTGGAGGTGCCTTGCCCCTCTCCTGCCCACCTCGGAATTTCCCTGTGGCTCCT
TTGATCCTTCGAGTCTCCAGCTCCTCTCCCTTCCACCTGTTTCCCCCAAGAAAGGCAGGA
TCCTGGTCCCTGCTACGTTTCTGGGGCCATGGCTGGTCTGGGCCCCGGCGTAGGCGATTC
AGAGGGGGGACCCCGGCCCCTGTTTTGCAGAAAGGGGGCTCTGAGGCAGAAGGTGGTCCA
CGAAGTCAAGAGCCACAAGTTCACCGCTCGCTTCTTCAAGCAGCCCACCTTCTGCAGCCA
CTGCACCGACTTCATCTGGGGTATCGGAAAGCAGGGCCTGCAATGTCAAGTCTGCAGCTT
TGTGGTTCATCGACGATGCCACGAATTTGTGACCTTCGAGTGTCCAGGCGCTGGGAAGGG
CCCCCAGACGGACGACCCCCGGAACAAACACAAGTTCCGCCTGCATAGCTACAGCAGCCC
CACCTTCTGCGACCACTGTGGCTCCCTCCTCTACGGGCTTGTGCACCAGGGCATGAAATG
CTCCTGCTGCGAGATGAACGTGCACCGGCGCTGTGTGCGTAGCGTGCCCTCCTGTGCGGT
GTGGACACACCGAGCGCCCGGCGCCTGCACTGGAGATCCCGGGCTCCACACAAAGAGATC
CACGTACTGTTGGCGAGGCCCGTACCTATTNCTATGACCCCACGTCTCTCTGTCCTATGT
GAACTGAGCTCANCCAGACCTCGAACTGACGACAGAGACCGACGTGAAGCAGCTAACCTG
TGTGAAGAAAN
>OriGene 3' read for NM_002739 unedited
NNNAAATTACTTGNACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTCAGC
ATCAGCATCCCAAATTTATTCTCCAGCAGCTGTGGCCGGGTGGGAGAGGCTAAGGGAAGA
GGAGGGGAGGAGGAGAGTCCCAGAGAGTGGCAGGCACTGGGGAGAGTTGGGGTGGGTCCG
AGCCAGACAGGAGCCATGCACGGCAGGCGGGCTGCGGGGAGAAGTGATGGCTTCGGGATG
GGGAAGTCTAGAACAAAAGCTGATTGGGAGCCTCTGGGGAAAGAATCCTCCATATATCCC
AGAAATCCCCAGAGCACAGCAGCGGGACGTACTGGAGGAGGGGGGCACGCCTCCCACTCA
TCTAGAACTAGAGTGGAGGCGGGGAGCCTCGGAGGCCGAGGGCTGTTCTGGAACCTGAGT
CTGGAACAGCCAACCCCGTGAGGCTATTTCCCCAGGGGCGGGGTCTGGGGGGAGGCACAG
AACTACCAAGATGGAGTCTCGAACCCCATGGAGTTGGGGATGGTTAGTGGTGTGGTCTCT
GGACCCGATTGGGGCAGAGTCCAGAACGCTAAGGTCAGGGAGAATATCGGGCTCCGCTCA
AGTCTTGAACGCGGGACGGCTGTAGAGGCTGTATGGAGTTCAGAAGGCCAGGAACGCTTG
GGAGGGGCGTCTAGAACCCGCGGGGGCAGAGCTGGAATGCTGGGGTGCAGGATCTAGAAT
GGGACAGGGGGTGGTGAAGTTGGGGTGGAAGTGGGGGCCTGGCCGCCNNGCACGCNGAGG
GGACGTTGGGGACACCTAGTGGCGGCGGGTGAGATACATGACGGGCACAGGCACTGGGCT
GGTGGGGCTGCCGGCATCCGGGTGCACGAAGTCGGGGTTCAACGTAGTGAAGCCCTGGAA
ATCGCCTGTTCGATGCTGGCCAGNAT
Restriction Sites NotI-NotI     
ACCN NM_002739
Insert Size 3500 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002739.3, NP_002730.1
RefSeq Size 3143 bp
RefSeq ORF 2094 bp
Locus ID 5582
Cytogenetics 19q13.42
Domains C2, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Calcium signaling pathway, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, Leukocyte transendothelial migration, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, Tight junction, Vascular smooth muscle contraction, VEGF signaling pathway, Vibrio cholerae infection, Wnt signaling pathway
Gene Summary 'Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]'
Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.

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