PKC gamma (PRKCG) (NM_002739) Human Untagged Clone

CAT#: SC112717

PRKCG (untagged)-Human protein kinase C, gamma (PRKCG)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKCG
• Synonyms: PKC-gamma; PKCC; PKCG; PKCgamma; PKCI(3); SCA14
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002739 edited
  ATGGCTGGTCTGGGCCCCGGCGTAGGCGATTCAGAGGGGGGACCCCGGCCCCTGTTTTGC
  AGAAAGGGGGCTCTGAGGCAGAAGGTGGTCCACGAAGTCAAGAGCCACAAGTTCACCGCT
  CGCTTCTTCAAGCAGCCCACCTTCTGCAGCCACTGCACCGACTTCATCTGGGGTATCGGA
  AAGCAGGGCCTGCAATGTCAAGTCTGCAGCTTTGTGGTTCATCGACGATGCCACGAATTT
  GTGACCTTCGAGTGTCCAGGCGCTGGGAAGGGCCCCCAGACGGACGACCCCCGGAACAAA
  CACAAGTTCCGCCTGCATAGCTACAGCAGCCCCACCTTCTGCGACCACTGTGGCTCCCTC
  CTCTACGGGCTTGTGCACCAGGGCATGAAATGCTCCTGCTGCGAGATGAACGTGCACCGG
  CGCTGTGTGCGTAGCGTGCCCTCCCTGTGCGGTGTGGACCACACCGAGCGCCGCGGGCGC
  CTGCAGCTGGAGATCCGGGCTCCCACAGCAGATGAGATCCACGTAACTGTTGGCGAGGCC
  CGTAACCTAATTCCTATGGACCCCAACGGTCTCTCTGATCCCTATGTGAAACTGAAGCTC
  ATCCCAGACCCTCGGAACCTGACGAAACAGAAGACCCGAACGGTGAAAGCCACGCTAAAC
  CCTGTGTGGAATGAGACCTTTGTGTTCAACCTGAAGCCAGGGGATGTGGAGCGCCGGCTC
  AGCGTGGAGGTGTGGGACTGGGACCGGACCTCCCGCAACGACTTCATGGGGGCCATGTCC
  TTTGGCGTCTCGGAGCTGCTCAAGGCGCCCGTGGATGGCTGGTACAAGTTACTGAACCAG
  GAGGAGGGCGAGTATTACAATGTGCCGGTGGCCGATGCTGACAACTGCAGCCTCCTCCAG
  AAGTTTGAGGCTTGTAACTACCCCCTGGAATTGTATGAGCGGGTGCGGATGGGCCCCTCT
  TCCTCTCCCATCCCCTCCCCTTCCCCTAGTCCCACCGACCCCAAGCGCTGCTTCTTCGGG
  GCGAGTCCAGGACGCCTGCACATCTCCGACTTCAGCTTCCTCATGGTTCTAGGAAAAGGC
  AGTTTTGGGAAGGTGATGCTGGCCGAGCGCAGGGGCTCTGATGAGCTCTACGCCATCAAG
  ATCTTGAAAAAGGACGTGATCGTCCAGGACGACGATGTGGACTGCACGCTGGTGGAGAAA
  CGTGTGCTGGCGCTGGGGGGCCGGGGTCCTGGCGGCCGGCCCCACTTCCTCACCCAGCTC
  CACTCCACCTTCCAGACCCCGGACCGCCTGTATTTCGTGATGGAGTACGTCACCGGGGGA
  GACTTGATGTACCACATTCAACAGCTGGGCAAGTTTAAGGAGCCCCATGCAGCGTTCTAC
  GCGGCAGAAATCGCTATCGGCCTCTTCTTCCTTCACAATCAGGGCATCATCTACAGGGAC
  CTGAAGCTGGACAATGTGATGCTGGATGCTGAGGGACACATCAAGATCACTGACTTTGGC
  ATGTGTAAGGAGAACGTCTTCCCCGGGACGACAACCCGCACCTTCTGCGGGACCCCGGAC
  TACATAGCCCCGGAGATCATTGCCTACCAGCCCTATGGGAAGTCTGTCGATTGGTGGTCC
  TTTGGAGTTCTGCTGTATGAGATGTTGGCAGGACAGCCTCCCTTCGATGGGGAGGACGAG
  GAGGAGCTGTTTCAGGCCATCATGGAACAAACTGTCACCTACCCCAAGTCGCTTTCCCGG
  GAAGCCGTGGCCATCTGCAAGGGGTTCCTGACCAAGCACCCAGGGAAGCGCCTGGGCTCA
  GGGCCTGATGGGGAACCTACCATCCGTGCACATGGCTTTTTCCGCTGGATTGACTGGGAG
  CGGCTGGAACGATTGGAGATCCCGCCTCCTTTCAGACCCCGCCCGTGTGGCCGCAGCGGC
  GAGAACTTTGACAAGTTCTTCACGCGGGCGGCGCCAGCGCTGACCCCTCCAGACCGCCTA
  GTCCTGGCCAGCATCGACCAGGCCGATTTCCAGGGCTTCACCTACGTGAACCCCGACTTC
  GTGCACCCGGATGCCCGCAGCCCCACCAGCCCAGTGCCTGTGCCCGTCATGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002739 unedited
  CCCCCGCCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTATATAAGCAG
  AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA
  TTCGGCACGAGGCCCTGGCGGAGCCGGCGCGCCCGGGGTGCCGCTCCCTGCCTGGCGCGC
  TCCGCACCTGGAGGTGCCTTGCCCCTCTCCTGCCCACCTCGGAATTTCCCTGTGGCTCCT
  TTGATCCTTCGAGTCTCCAGCTCCTCTCCCTTCCACCTGTTTCCCCCAAGAAAGGCAGGA
  TCCTGGTCCCTGCTACGTTTCTGGGGCCATGGCTGGTCTGGGCCCCGGCGTAGGCGATTC
  AGAGGGGGGACCCCGGCCCCTGTTTTGCAGAAAGGGGGCTCTGAGGCAGAAGGTGGTCCA
  CGAAGTCAAGAGCCACAAGTTCACCGCTCGCTTCTTCAAGCAGCCCACCTTCTGCAGCCA
  CTGCACCGACTTCATCTGGGGTATCGGAAAGCAGGGCCTGCAATGTCAAGTCTGCAGCTT
  TGTGGTTCATCGACGATGCCACGAATTTGTGACCTTCGAGTGTCCAGGCGCTGGGAAGGG
  CCCCCAGACGGACGACCCCCGGAACAAACACAAGTTCCGCCTGCATAGCTACAGCAGCCC
  CACCTTCTGCGACCACTGTGGCTCCCTCCTCTACGGGCTTGTGCACCAGGGCATGAAATG
  CTCCTGCTGCGAGATGAACGTGCACCGGCGCTGTGTGCGTAGCGTGCCCTCCTGTGCGGT
  GTGGACACACCGAGCGCCCGGCGCCTGCACTGGAGATCCCGGGCTCCACACAAAGAGATC
  CACGTACTGTTGGCGAGGCCCGTACCTATTNCTATGACCCCACGTCTCTCTGTCCTATGT
  GAACTGAGCTCANCCAGACCTCGAACTGACGACAGAGACCGACGTGAAGCAGCTAACCTG
  TGTGAAGAAAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002739 unedited
  NNNAAATTACTTGNACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTCAGC
  ATCAGCATCCCAAATTTATTCTCCAGCAGCTGTGGCCGGGTGGGAGAGGCTAAGGGAAGA
  GGAGGGGAGGAGGAGAGTCCCAGAGAGTGGCAGGCACTGGGGAGAGTTGGGGTGGGTCCG
  AGCCAGACAGGAGCCATGCACGGCAGGCGGGCTGCGGGGAGAAGTGATGGCTTCGGGATG
  GGGAAGTCTAGAACAAAAGCTGATTGGGAGCCTCTGGGGAAAGAATCCTCCATATATCCC
  AGAAATCCCCAGAGCACAGCAGCGGGACGTACTGGAGGAGGGGGGCACGCCTCCCACTCA
  TCTAGAACTAGAGTGGAGGCGGGGAGCCTCGGAGGCCGAGGGCTGTTCTGGAACCTGAGT
  CTGGAACAGCCAACCCCGTGAGGCTATTTCCCCAGGGGCGGGGTCTGGGGGGAGGCACAG
  AACTACCAAGATGGAGTCTCGAACCCCATGGAGTTGGGGATGGTTAGTGGTGTGGTCTCT
  GGACCCGATTGGGGCAGAGTCCAGAACGCTAAGGTCAGGGAGAATATCGGGCTCCGCTCA
  AGTCTTGAACGCGGGACGGCTGTAGAGGCTGTATGGAGTTCAGAAGGCCAGGAACGCTTG
  GGAGGGGCGTCTAGAACCCGCGGGGGCAGAGCTGGAATGCTGGGGTGCAGGATCTAGAAT
  GGGACAGGGGGTGGTGAAGTTGGGGTGGAAGTGGGGGCCTGGCCGCCNNGCACGCNGAGG
  GGACGTTGGGGACACCTAGTGGCGGCGGGTGAGATACATGACGGGCACAGGCACTGGGCT
  GGTGGGGCTGCCGGCATCCGGGTGCACGAAGTCGGGGTTCAACGTAGTGAAGCCCTGGAA
  ATCGCCTGTTCGATGCTGGCCAGNAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002739
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002739.3, NP_002730.1
• RefSeq Size: 3143 bp
• RefSeq ORF: 2094 bp
• Locus ID: 5582
• Cytogenetics: 19q13.42
• Domains: C2, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Calcium signaling pathway, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, Leukocyte transendothelial migration, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Natural killer cell mediated cytotoxicity, Non-small cell lung cancer, Pathways in cancer, Phosphatidylinositol signaling system, Tight junction, Vascular smooth muscle contraction, VEGF signaling pathway, Vibrio cholerae infection, Wnt signaling pathway
• Gene Summary: 'Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]'
  Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1.