Glycogen synthase 2 (GYS2) (NM_021957) Human Untagged Clone

CAT#: SC112826

GYS2 (untagged)-Human glycogen synthase 2 (liver) (GYS2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GYS2
• Synonyms: glycogen synthase 2 (liver)
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112826 sequence for NM_021957 edited (data generated by NextGen Sequencing)
  ATGCTTCGAGGCCGATCCCTCTCTGTAACATCCCTGGGTGGGCTTCCCCAGTGGGAAGTC
  GAAGAACTTCCTGTGGAGGAGTTACTGCTCTTTGAAGTTGCTTGGGAAGTGACCAATAAA
  GTTGGAGGCATCTATACTGTGATTCAGACAAAGGCCAAAACAACAGCAGATGAATGGGGA
  GAGAACTATTTTCTGATAGGTCCATATTTTGAGCATAATATGAAGACTCAGGTGGAACAG
  TGTGAACCTGTAAATGATGCTGTCAGAAGAGCAGTGGACGCAATGAATAAGCATGGCTGC
  CAGGTGCATTTTGGAAGATGGCTGATAGAAGGAAGTCCTTATGTGGTACTTTTTGACATA
  GGCTATTCAGCTTGGAATCTGGACAGGTGGAAGGGTGACCTCTGGGAAGCATGCAGTGTC
  GGCATTCCTTATCATGACCGAGAAGCCAATGATATGCTGATATTTGGATCTTTAACTGCC
  TGGTTCTTAAAAGAGGTGACAGATCATGCAGATGGTAAATATGTCGTTGCCCAATTCCAT
  GAATGGCAGGCTGGAATTGGACTGATCCTTTCTCGAGCCAGGAAACTTCCTATTGCCACA
  ATATTTACAACCCACGCTACACTACTTGGGAGGTATCTCTGTGCAGCAAATATTGATTTC
  TACAACCATCTTGATAAGTTTAACATTGACAAAGAGGCTGGGGAAAGGCAGATTTACCAC
  CGGTACTGCATGGAGCGAGCTTCCGTTCATTGCGCTCACGTGTTCACCACGGTTTCTGAA
  ATAACAGCAATAGAAGCTGAACATATGCTGAAGAGAAAGCCTGATGTAGTTACTCCAAAC
  GGCTTGAATGTTAAGAAATTTTCAGCAGTGCATGAGTTTCAAAATCTACATGCCATGTAC
  AAGGCCAGAATCCAAGATTTTGTTCGAGGTCATTTCTATGGTCATCTCGACTTTGATCTT
  GAAAAGACTTTGTTCCTTTTCATTGCTGGGAGGTATGAGTTTTCAAACAAAGGAGCTGAC
  ATCTTCCTAGAATCCTTATCCAGGCTAAATTTCCTGCTGAGGATGCATAAAAGTGACATC
  ACAGTGGTGGTGTTTTTCATTATGCCTGCCAAGACAAATAATTTCAACGTGGAAACCCTG
  AAAGGACAAGCAGTGCGAAAACAGCTGTGGGATGTTGCACATTCTGTGAAGGAAAAGTTT
  GGAAAAAAACTCTATGATGCATTATTAAGAGGAGAAATTCCTGACCTGAACGATATTTTA
  GATCGAGATGATCTAACAATTATGAAAAGAGCCATCTTTTCAACTCAGCGACAGTCATTG
  CCCCCAGTGACCACGCACAACATGATTGATGACTCCACCGACCCCATCCTCAGCACCATT
  AGACGGATCGGACTTTTCAACAACCGCACAGATAGAGTCAAGGTGATTTTGCACCCAGAG
  TTTCTATCCTCCACCAGTCCCTTACTACCCATGGACTATGAAGAGTTTGTTAGAGGTTGT
  CATCTTGGAGTATTTCCATCATACTATGAACCCTGGGGTTATACTCCAGCTGAATGCACT
  GTGATGGGTATCCCCAGTGTGACCACGAATCTCTCCGGGTTTGGCTGTTTCATGCAGGAG
  CACGTGGCTGATCCTACTGCTTACGGTATTTACATCGTTGACAGGCGGTTCCGTTCTCCA
  GATGATTCTTGCAATCAGCTGACTAAGTTTCTCTATGGATTTTGCAAACAGTCACGCCGC
  CAAAGGATTATCCAGAGGAACAGAACTGAGAGGCTCTCAGATCTTCTGGATTGGAGATAC
  TTAGGCAGATATTACCAGCATGCCAGACACCTGACATTAAGCAGAGCTTTTCCAGATAAA
  TTCCATGTGGAACTAACATCACCACCAACGACAGAAGGATTTAAATATCCCAGGCCTTCC
  TCAGTACCACCTTCTCCTTCAGGGTCTCAGGCCTCCAGTCCTCAGAGCAGTGATGTGGAA
  GATGAAGTGGAGGATGAGAGATACGATGAGGAAGAGGAGGCTGAAAGGGATCGGTTAAAT
  ATCAAGTCACCATTTTCACTGAGCCACGTTCCTCATGGGAAGAAAAAGCTGCATGGTGAA
  TATAAGAACTGA
  
  Clone variation with respect to NM_021957.3
  1087 a=>g;1389 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021957 unedited
  ATAACGTCGCCAATTGTTAACCATCATATAGGCGGCCCGAATCGCACCGGAAAGAGAGAA
  AAAGGAGGAAGATTCCTCCTTCCACCAGGAATTCTGTGGGAAGCACATAAGATTTCATGC
  TACTAGTTTATTCCCAAGAGAAGCTACCAAAGCCTGGTAACTCTACCAACTCTAACTTTT
  GTGCCTGTAAGTTCTCTTCTCCTGGGATTACAACTAATTGAAACAGGAATTCAAGGAGTC
  TCGGAGGACTGTAAGAAGAATGCTTCGAGGCCGATCCCTCTCTGTAACATCCCTGGGTGG
  GCTTCCCCAGTGGGAAGTCGAAGAACTTCCTGTGGAGGAGTTACTGCTCTTTGAAGTTGC
  TTGGGAAGTGACCAATAAAGTTGGAGGCATCTATACTGTGATTCAGACAAAGGCCAAAAC
  AACAGCAGATGAATGGGGAGAGAACTATTTTCTGATAGGTCCATATTTTGAGCATAATAT
  GAAGACTCAGGTGGAACAGTGTGAACCTGTAAATGATGCTGTCAGAAGAGCAGTGGACGC
  AATGAATAAGCATGGCTGCCAGGTGCATTTTGGAAGATGGCTGATAGAAGGAAGTCCTTA
  TGTGGTACTTTTTGACATAGGCTATTCAGCTTGGAATCTGGNACAGGTGGAAAGGTGACC
  TCTGGGAAGCATGCAGTGTCNGCATTCCTTATCATGACCGAGNAAGCAATGATATGCTGA
  TATTTGGATCTTTTACTGCCTGGTTNCTAAAAGAAGTGACAGATCATGCAGATGGTAAAA
  TATGTCGTTGCCCATTCCATGAATGGCAGGCTGGAATTGNACTGATTCTTTCTCGAGCCA
  GGAACCTTCTATTGCCCCATTATTTACACCCACGCTACAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021957 unedited
  NTTTTACTCTGNNACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTAA
  GTTATTAAATATTAATGTGTTTATTTACTTGGATTTTACAAGTTATCATCTATGAAGATT
  GACCAAATTTTAAAAAGGCTTTCTATTTAAACAATAAAAAAATGTTACAGTAAGATGGTG
  GTACCATGTACATGGTAATATCTTGCATTAATTACAACATCAATTCTAGATTTCAAAAGT
  GACATAAATGTCCATTAATATGCATCAAAATATGGACTTAGGATAGCTATTCACTCATGG
  ATTTTTGTCTCTGCAAAACTACATCTAAATCATGGTTCTGATGCATGTGAAAAATGAAAA
  TAATCAGTAAAAACCTAGCTATTTAAAAAATGTAGGTAGTGCACATTCATGGGTAAGATC
  AAGGTTTTCGGGGGGGAAATGGGAAATTTGTGTGGTGGGGTACACAAGCTGCATAAATAG
  TAAGCAAAGGATTATGATGATCATTTAAAAATAAACAGAGTAAGAGAAAATCCTTACCAC
  TTAAATTCACCATTTTAAAAACACTTTTCCGTCTTTCTGGCCTTTAATGAGTGCTCCTCC
  TCATGCCTAACTTTATGGGGGAAACAAGAGTTGGGGAAAATAACTTGGATCTTATCCTAA
  ATTACAAAATTATCATTCACTCAATTTCTTCCACTTTTTAGGCAGAGAATAAACTCCATT
  GTAATACTTAGAAGGAGAAAATGANATTTGTGGCATTTTTATTTTAAATAATTAGTCTTA
  CTTTGCTTTTTTAAATTAGCTCTTCATGCAGCACATGTAGAAATCAGNTCTTATATTCAC
  CATGCAGCTTTTTCTTCCATGANGAACGTGNCTAGTGAAAATGTGACTTGATATTAACCG
  ATCCCTTNAGNCTGCTCTTCTCACGNATCTCTATCCTCCACTTATCTTCACACACTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021957
• Insert Size: 2930 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021957.2, NP_068776.1
• RefSeq Size: 2912 bp
• RefSeq ORF: 2112 bp
• Locus ID: 2998
• Cytogenetics: 12p12.1
• Protein Pathways: Insulin signaling pathway, Starch and sucrose metabolism
• Gene Summary: 'The protein encoded by this gene, liver glycogen synthase, catalyzes the rate-limiting step in the synthesis of glycogen - the transfer of a glucose molecule from UDP-glucose to a terminal branch of the glycogen molecule. Mutations in this gene cause glycogen storage disease type 0 (GSD-0) - a rare type of early childhood fasting hypoglycemia with decreased liver glycogen content. [provided by RefSeq, Dec 2009]'