NMT1 (NM_021079) Human Untagged Clone

CAT#: SC113026

NMT1 (untagged)-Human N-myristoyltransferase 1 (NMT1)


  "NM_021079" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NMT1
Synonyms NMT
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC113026 sequence for NM_021079 edited (data generated by NextGen Sequencing)
ATGGCGGACGAGAGTGAGACAGCAGTGAAGCCGCCGGCACCTCCGCTGCCGCAGATGATG
GAAGGGAACGGGAACGGCCATGAGCACTGCAGCGATTGCGAGAATGAGGAGGACAACAGC
TACAACCGGGGTGGTTTGAGTCCAGCCAATGACACTGGAGCCAAAAAGAAGAAAAAGAAA
CAAAAAAAGAAGAAAGAAAAAGGCAGTGAGACAGATTCAGCCCAGGATCAGCCTGTGAAG
ATGAACTCTTTGCCAGCAGAGAGGATCCAGGAAATACAGAAGGCCATTGAGCTGTTCTCA
GTGGGTCAGGGACCTGCCAAAACCATGGAGGAGGCTAGCAAGCGAAGCTACCAGTTCTGG
GATACGCAGCCCGTCCCCAAGCTGGGCGAAGTGGTGAACACCCATGGCCCCGTGGAGCCT
GACAAGGACAATATCCGCCAGGAGCCCTACACCCTGCCCCAGGGCTTCACCTGGGATGCT
TTGGACCTGGGCGATCGTGGTGTGCTAAAAGAACTGTACACCCTCCTGAATGAGAACTAT
GTGGAAGATGATGACAACATGTTCCGATTTGATTATTCCCCGGAGTTTCTTTTGTGGGCT
CTCCGGCCACCCGGCTGGCTCCCCCAGTGGCACTGTGGGGTTCGAGTGGTCTCAAGTCGG
AAATTGGTTGGGTTCATTAGCGCCATCCCAGCAAACATCCATATCTATGACACAGAGAAG
AAGATGGTAGAGATCAACTTCCTGTGTGTCCACAAGAAGCTGCGTTCCAAGAGGGTTGCT
CCAGTTCTGATCCGAGAGATCACCAGGCGGGTTCACCTGGAGGGCATCTTCCAAGCAGTT
TACACTGCCGGGGTGGTACTACCAAAGCCCGTTGGCACCTGCAGGTATTGGCATCGGTCC
CTAAACCCACGGAAGCTGATTGAAGTGAAGTTCTCCCACCTGAGCAGAAATATGACCATG
CAGCGCACCATGAAGCTCTACCGACTGCCAGAGACTCCCAAGACAGCTGGGCTGCGACCA
ATGGAAACAAAGGACATTCCAGTAGTGCACCAGCTCCTCACCAGGTACTTGAAGCAATTT
CACCTTACGCCCGTCATGAGCCAGGAGGAGGTGGAGCACTGGTTCTACCCCCAGGAGAAT
ATCATCGACACTTTCGTGGTGGAGAACGCAAACGGAGAGGTGACAGATTTCCTGAGCTTT
TATACGCTGCCCTCCACCATCATGAACCATCCAACCCACAAGAGTCTCAAAGCTGCTTAT
TCTTTCTACAACGTTCACACCCAGACCCCTCTTCTAGACCTCATGAGCGACGCCCTTGTC
CTCGCCAAAATGAAAGGGTTTGATGTGTTCAATGCACTGGATCTCATGGAGAACAAAACC
TTCCTGGAGAAGCTCAAGTTTGGCATAGGGGACGGCAACCTGCAGTATTACCTTTACAAT
TGGAAATGCCCCAGCATGGGGGCAGAGAAGGTTGGACTGGTGCTACAATAA

Clone variation with respect to NM_021079.3
487 t=>c
>OriGene 5' read for NM_021079 unedited
NAAAGGTTCAAATTTAGTATACGACTCATTATAGGCGGCCGCGCCTCGTGCCGAATTCGG
CACGAGGGAGCCCTGCTCTCGCACTCAAGAGGCGGACGAGAGTGAGACAGCAGTGAAGCC
GCCGGCACCTCCGCTGCCGCAGATGATGGAAGGGAACGGGAACGGCCATGAGCACTGCAG
CGATTGCGAGAATGAGGAGGACAACAGCTACAACCGGGGTGGTTTGAGTCCAGCCAATGA
CACTGGAGCCAAAAAGAAGAAAAAGAAACAAAAAAAGAAGAAAGAAAAAGGCAGTGAGAC
AGATTCAGCCCAGGATCAGCCTGTGAAGATGAACTCTTTGCCAGCAGAGAGGATCCAGGA
AATACAGAAGGCCATTGAGCTGTTCTCAGTGGGTCAGGGACCTGCCAAAACCATGGAGGA
GGCTAGCAAGCGAAGCTACCAGTTCTGGGATACGCAGCCCGTCCCCAAGCTGGGCGAAGT
GGTGAACACCCATGGCCCCGTGGAGCCTGACAAGGACAATATCCGCCAGGAGCCCTACAC
CCTGCCCCAGGGCTTCACCTGNGATGCTTTGGACCTGNGCGATCGTGGTGTGCTAAAAGA
ACTGTACACCCTCCTGAATGAGAACTATGTGGAAGATGATGACAACATGTTCCGATTTGA
TTATTCCCCGGAGTTTCTTTTGTGGGCTCTCCGGCCACCCGGCTGGCTCCCCCAGGGACT
GTGTGGGGGTCGAGTGGTCTCAAGTCGGAAATTGTNTGGGNTCATTAGCGCCATCCCAGC
AAACATCCATATCTATGACACAGAGAAGAAGAGGTNGAGATCAACTTCTGTGTGTNCACA
AGAGCTGCNGTCCAGAAGGGTGCTCCAGTTCTGATCCGAGAGATCACCAGCGGGTCACCT
GGAAGGCATCT
>OriGene 3' read for NM_021079 unedited
GGCACGCAATCTAGTATCGAGTTTTTTTTTTTTTTTTTTAATATATATATATACTTTTAT
TATTCACCCGTTAACTCCATTATATGCCAATCACGAGCTAGTTTTCAGCAGTTACATTCC
CTTGATCACGGCTGCATGAGGATGTAATTAACTGGAAAACACGGAGACCAGAGACACGAC
CGGAGGTGACGTCACGCAGCCCACGCCATCGCAATACAATTGTCAAGTTCGCTGGCGGTT
TGGTAAAGCCGGTTCAGTTCTGCTCCCTTTGCCAGGGATTCTCACGTGTGTGAAAATTGG
ACCAACAGTGGTGGGGTTCCATTTCCTGGTTCGAATTTTCAGTGGCTTTATCCAGAATCG
CACTGGTGACTGGTTATTGTAGCACCAGTCCAACCTTCTCTGCCCCCATGCTGGGGCATT
TCCAATTGTAAAGGTAATACTGCAGGTTGCCGTCCCCTATGCCAAACTTGAGCTTCTCCA
GGAAGGTTTTGTTCTCCATGAGATCCAGTGCATTGAACACATCAAACCCTTTCATTTTGG
CGAGGACAAGGGCGTCGCTCATGAAGTCTATAAGAGGGGTCTGGGTGTGAACGTTGTANA
AAGAATAAGCAGCTTTGAGACTCTTGTGGGTTGGATGGTTCATGATGGTGGAGGGCAGCG
TATAAAAGCTCAGGATATCTGTCACCTCTCCGTTTGCGTTCTCCACCACGAAAGTGTCGA
TGATATTCTCCTGAGGGTAGAACCAGAGCTCCACCTCCTCCTGGCTCATGACGGNCGTAA
AGTGAAATTGCCTCAAGTACCTGGTGAGGAGCTGGTGCACTACTGGAATGTNCTTTGNTT
NCATTGGTCGCAGNCCANCTGTCTTGGGAGTCTCTGGGCAGTCGTAAACCTTATGGAGCG
CTGCATGGNCATATTCTGCTCAGNAGGGAGAACTCACTTCATCAGCTCCGGGGGGTTAGG
GACGATGCCATACCTGCAGTGCCNACGGNCTTN
Restriction Sites NotI-NotI     
ACCN NM_021079
Insert Size 1870 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_021079.3, NP_066565.1
RefSeq Size 4903 bp
RefSeq ORF 1491 bp
Locus ID 4836
Cytogenetics 17q21.31
Domains NMT
Protein Families Druggable Genome
Gene Summary 'Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM, Nov 2008]'

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