Ubiquitin (UBB) (NM_018955) Human Untagged Clone

CAT#: SC113349

UBB (untagged)-Human ubiquitin B (UBB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBB
• Synonyms: HEL-S-50
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113349 sequence for NM_018955 edited (data generated by NextGen Sequencing)
  ATGCAGATCTTCGTGAAAACCCTTACCGGCAAGACCATCACCCTTGAGGTGGAGCCCAGT
  GACACCATCGAAAATGTGAAGGCCAAGATCCAGGATAAGGAAGGCATTCCCCCCGACCAG
  CAGAGGCTCATCTTTGCAGGCAAGCAGCTGGAAGATGGCCGTACTCTTTCTGACTACAAC
  ATCCAGAAGGAGTCGACCCTGCACCTGGTCCTGCGTCTGAGAGGTGGTATGCAGATCTTC
  GTGAAGACCCTGACCGGCAAGACCATCACCCTGGAAGTGGAGCCCAGTGACACCATCGAA
  AATGTGAAGGCCAAGATCCAGGATAAAGAAGGCATCCCTCCCGACCAGCAGAGGCTCATC
  TTTGCAGGCAAGCAGCTGGAAGATGGCCGCACTCTTTCTGACTACAACATCCAGAAGGAG
  TCGACCCTGCACCTGGTCCTGCGTCTGAGAGGTGGTATGCAGATCTTCGTGAAGACCCTG
  ACCGGCAAGACCATCACTCTGGAGGTGGAGCCCAGTGACACCATCGAAAATGTGAAGGCC
  AAGATCCAAGATAAAGAAGGCATCCCCCCCGACCAGCAGAGGCTCATCTTTGCAGGCAAG
  CAGCTGGAAGATGGCCGCACTCTTTCTGACTACAACATCCAGAAAGAGTCGACCCTGCAC
  CTGGTCCTGCGCCTGAGGGGTGGCTGTTAA
  
  Clone variation with respect to NM_018955.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018955 unedited
  TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCCCTGTGGGTGGAC
  GTGGTTGGTGATTGGCAGGATCCTGGTATCCGCTAACAGGTCAAAATGCAGATCTTCGTG
  AAAACCCTTACCGGCAAGACCATCACCCTTGAGGTGGAGCCCAGTGACACCATCGAAAAT
  GTGAAGGCCAAGATCCAGGATAAGGAAGGCATTCCCCCCGACCAGCAGAGGCTCATCTTT
  GCAGGCAAGCAGCTGGAAGATGGCCGTACTCTTTCTGACTACAACATCCAGAAGGAGTCG
  ACCCTGCACCTGGTCCTGCGTCTGAGAGGTGGTATGCAGATCTTCGTGAAGACCCTGACC
  GGCAAGACCATCACCCTGGAAGTGGAGCCCAGTGACACCATCGAAAATGTGAAGGCCAAG
  ATCCAGGATAAAGAAGGCATCCCTCCCGACCAGCAGAGGCTCATCTTTGCAGGCAAGCAG
  CTGGAAGATGGCCGCACTCTTTCTGACTACAACATCCAGAAGGAGTCGACCCTGCACCTG
  GTCCTGCGTCTGAGAGGTGGTATGCAGATCTTCGTGAAGACCCTGACCGGCAAGACCATC
  ACTCTGGAGGTGGAGCCCAGTGACACCATCGAAAATGTGAAGGCCAAGATCCAAGATAAA
  GAAGGCATCCCCCCCGACCAGCAGAGGCTCATCTTTGCAGGCAAGCAGCTGGAAGATGGC
  CGCACTCTTTCTGACTACAACATCCAGAAAGAGTCGACCCTGCACCTGGTCCTGCGCCTG
  AGGGGTGGCTGNTAATTCTTCAGTCATGGCATTCGCAGTGCCCAGTGATGGCATTACTCT
  GCACTATAGCCATTTGCCCCAACTAAAAGTTAGAATACAGNTNCAGTATAGCTGAACCTG
  TCAAATGTATAAAGGTTCGTGCATGTAGCAAAAAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018955 unedited
  NAATACTGTGNACCGCGGCCGCATTCTANNGATCGGTTTTTTCTTTTTTTTTTTGCTACC
  ATGCAACGAAACCTTTATTAACATTTTGAACAGGTTCAGCTATTACTGAAACTTGTAATT
  TCTAAACTTAAGTTGGGGCAAATGGCTATAGTGCAGAGTAATGCCATCACTGGGCACTGC
  GAATGCCATGACTGAAGAATTAACAGCCACCCCTTATGCGCAGGACCAGGTGCAGGGTCG
  ACTCTTTCTGGATGTTGTAGTCAGAAAGAGTGCGGCCATCTTCCAGCTGCTTGCCTGCAA
  AGATGAGCCTCTGCTGGTCGGGGGGGATGCCTTCTTTATCTTGGATCTTGGCCTTCACAT
  TTTCGATGGTGTCACTGGGCTCCACCTCCAGAGTGATGGTCTTGCCGGTCAGGGTCTTCA
  CGAAGATCTGCATACCACCTCTCAGACGCAGGACCAGGTGCAGGGTCGACTCCTTCTGGA
  TGTTGTAGTCAGAAAGAGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATGAGCCTCT
  GCTGGTCGGGAGGGATGCCTTCTTTATCCTGGATCTTGGCCTTCACATTTTCGATGGTGT
  CACTGGGCTCCACTTCCAGGGTGATGGTCTTGCCGGTCAGGGTCTTCACGAAGATCTGCA
  TACCACCTCTCAGACGCANGACCAGGTGCAGGGTCGACTCCTTCTGGATGTTGTAGTCAG
  AAAAGTACGGCCATCTTCCAGCTGCTTGCCTGCAAAGATGAGCCTCTGCTGGTCGGGGGG
  GATGCCTTNCCTATCCTGGATCTGGNCCTTCACATTTTCGAGGTGTCACTGGGCTTCAAC
  TCCAAGGTGATGGTCTTGCCGTTAGGGTTTTCACGAAAATCTGCATTTGACCTGTTACCG
  AAACCAGGATACTGGCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018955
• Insert Size: 1000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018955.2, NP_061828.1
• RefSeq Size: 971 bp
• RefSeq ORF: 690 bp
• Locus ID: 7314
• Cytogenetics: 17p11.2
• Domains: UBQ
• Protein Families: Druggable Genome
• Protein Pathways: Parkinson's disease
• Gene Summary: 'This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]'
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, 3, 4, 5, and 6 encode the same protein.