NGLY1 (NM_018297) Human Untagged Clone

CAT#: SC113603

NGLY1 (untagged)-Human N-glycanase 1 (NGLY1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NGLY1
• Synonyms: CDDG; CDG1V; PNG1; PNGase
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018297, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGCGGCATTGGGCAGCTCCTCAGGCTCGGCGTCCCCGGCCGTGGCTGAGCTCTGCCAGAACA
  CCCCGGAGACCTTTTTGGAGGCCTCCAAGCTGCTGCTCACCTATGCTGACAACATCCTCAGAAACCCTAA
  TGATGAAAAATATAGATCCATCCGGATTGGAAACACAGCCTTTTCTACTAGACTCTTGCCTGTCAGAGGA
  GCTGTTGAATGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACACATCTCATCTTTCCTAAAAAAGCTT
  CAGTGGAGCAGCTGCAAAAAATTCGTGACCTGATTGCCATAGAGAGAAGTAGCAGACTGGATGGCTCAAA
  TAAGAGCCACAAAGTAAAGTCATCTCAGCAACCTGCAGCCAGTACCCAGCTTCCTACAACACCATCTTCA
  AATCCCAGTGGGTTAAACCAGCACACAAGGAACCGTCAAGGGCAGTCATCAGATCCACCATCTGCTTCAA
  CGGTTGCTGCTGACTCAGCCATTCTAGAAGTTCTTCAGTCCAACATTCAGCATGTGCTGGTCTATGAAAA
  TCCTGCTCTTCAGGAGAAAGCGTTGGCTTGTATTCCGGTCCAAGAACTAAAAAGGAAATCACAAGAAAAG
  TTATCGAGAGCTAGAAAATTGGATAAAGGTATCAATATAAGTGATGAGGATTTTCTTTTGCTGGAGCTTT
  TGCACTGGTTTAAGGAAGAATTTTTTCACTGGGTGAATAACGTTTTGTGCAGCAAATGTGGTGGACAGAC
  TAGGTCTAGAGATAGATCATTACTGCCCAGTGATGATGAGCTGAAGTGGGGTGCAAAGGAAGTGGAAGAT
  CATTACTGTGATGCCTGCCAGTTCAGCAATCGATTCCCAAGATATAATAACCCTGAGAAACTTTTGGAAA
  CAAGATGTGGACGGTGTGGCGAGTGGGCCAATTGTTTTACACTGTGCTGCCGAGCTGTAGGGTTTGAAGC
  TCGCTATGTTTGGGATTACACAGACCATGTCTGGACAGAAGTCTATTCTCCTTCTCAGCAGCGGTGGCTG
  CACTGTGATGCATGTGAAGATGTCTGTGACAAGCCACTCCTTTATGAAATAGGATGGGGCAAGAAGCTTT
  CCTATGTCATAGCATTTTCAAAAGATGAGGTAGTTGATGTCACTTGGCGATATTCCTGCAAACATGAAGA
  GGTGATTGCCAGAAGAACTAAGGTTAAAGAAGCATTACTTCGAGACACTATTAATGGGCTTAATAAGCAG
  AGGCAACTGTTTTTGTCAGAAAACAGAAGGAAAGAACTTCTCCAGAGGATAATTGTGGAGCTTGTTGAAT
  TTATATCTCCCAAAACCCCTAAACCTGGAGAACTTGGGGGAAGAATATCTGGGTCAGTGGCTTGGAGAGT
  AGCCCGAGGTGAAATGGGTCTACAGAGAAAAGAAACCTTGTTTATTCCCTGTGAAAATGAGAAGATTTCT
  AAACAGCTCCACCTTTGTTACAATATTGTGAAAGATCGTTATGTTCGAGTTTCAAATAACAATCAAACCA
  TTTCTGGATGGGAGAATGGCGTGTGGAAAATGGAATCTATATTCAGAAAAGTTGAAACAGACTGGCACAT
  GGTATATTTGGCCCGAAAGGAAGGATCATCTTTTGCTTATATTTCCTGGAAGTTTGAGTGTGGGTCAGTT
  GGCCTAAAAGTAGATAGCATTTCTATTAGAACAAGTAGTCAAACTTTTCAGACTGGAACAGTAGAATGGA
  AATTGCGATCTGATACAGCACAAGTAGAACTGACAGGCGATAACAGTCTTCACTCCTATGCTGATTTTTC
  TGGTGCCACTGAAGTTATTTTGGAAGCAGAATTAAGCAGAGGAGATGGTGATGTCGCTTGGCAACACACC
  CAGCTGTTTAGACAAAGCTTAAATGACCATGAAGAAAATTGTTTGGAGATAATTATAAAATTCAGTGACC
  TTTGA
  
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018297
• ORF Size: 1965 bp
• Insert Size: 2440
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_018297.2, NP_060767.2
• RefSeq Size: 2182
• RefSeq ORF: 1965
• Locus ID: 55768
• Domains: TGc, PUG, PAW
• Gene Summary: This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.