NGLY1 (NM_018297) Human Untagged Clone

CAT#: SC320763

NGLY1 (untagged)-Human N-glycanase 1 (NGLY1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NGLY1
• Synonyms: CDDG; CDG1V; PNG1; PNGase
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_018297.2 GGAGAGCGAGGGGCGGCGGCGGCGCCCGCTGGCGCTCAAGCATGGCGGCGGCGGCATTGG
  GCAGCTCCTCAGGCTCGGCGTCCCCGGCCGTGGCTGAGCTCTGCCAGAACACCCCGGAGA
  CCTTTTTGGAGGCCTCCAAGCTGCTGCTCACCTATGCTGACAACATCCTCAGAAACCCTA
  ATGATGAAAAATATAGATCCATCCGGATTGGAAACACAGCCTTTTCTACTAGACTCTTGC
  CTGTCAGAGGAGCTGTTGAATGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACACATC
  TCATCTTTCCTAAAAAAGCTTCAGTGGAGCAGCTGCAAAAAATTCGTGACCTGATTGCCA
  TAGAGAGAAGTAGCAGACTGGATGGCTCAAATAAGAGCCACAAAGTAAAGTCATCTCAGC
  AACCTGCAGCCAGTACCCAGCTTCCTACAACACCATCTTCAAATCCCAGTGGGTTAAACC
  AGCACACAAGGAACCGTCAAGGGCAGTCATCAGATCCACCATCTGCTTCAACGGTTGCTG
  CTGACTCAGCCATTCTAGAAGTTCTTCAGTCCAACATTCAGCATGTGCTGGTCTATGAAA
  ATCCTGCTCTTCAGGAGAAAGCGTTGGCTTGTATTCCGGTCCAAGAACTAAAAAGGAAAT
  CACAAGAAAAGTTATCGAGAGCTAGAAAATTGGATAAAGGTATCAATATAAGTGATGAGG
  ATTTTCTTTTGCTGGAGCTTTTGCACTGGTTTAAGGAAGAATTTTTTCACTGGGTGAATA
  ACGTTTTGTGCAGCAAATGTGGTGGACAGACTAGGTCTAGAGATAGATCATTACTGCCCA
  GTGATGATGAGCTGAAGTGGGGTGCAAAGGAAGTGGAAGATCATTACTGTGATGCCTGCC
  AGTTCAGCAATCGATTCCCAAGATATAATAACCCTGAGAAACTTTTGGAAACAAGATGTG
  GACGGTGTGGCGAGTGGGCCAATTGTTTTACACTGTGCTGCCGAGCTGTAGGGTTTGAAG
  CTCGCTATGTTTGGGATTACACAGACCATGTCTGGACAGAAGTCTATTCTCCTTCTCAGC
  AGCGGTGGCTGCACTGTGATGCATGTGAAGATGTCTGTGACAAGCCACTCCTTTATGAAA
  TAGGATGGGGCAAGAAGCTTTCCTATGTCATAGCATTTTCAAAAGATGAGGTAGTTGATG
  TCACTTGGCGATATTCCTGCAAACATGAAGAGGTGATTGCCAGAAGAACTAAGGTTAAAG
  AAGCATTACTTCGAGACACTATTAATGGGCTTAATAAGCAGAGGCAACTGTTTTTGTCAG
  AAAACAGAAGGAAAGAACTTCTCCAGAGGATAATTGTGGAGCTTGTTGAATTTATATCTC
  CCAAAACCCCTAAACCTGGAGAACTTGGGGGAAGAATATCTGGGTCAGTGGCTTGGAGAG
  TAGCCCGAGGTGAAATGGGTCTACAGAGAAAAGAAACCTTGTTTATTCCCTGTGAAAATG
  AGAAGATTTCTAAACAGCTCCACCTTTGTTACAATATTGTGAAAGATCGTTATGTTCGAG
  TTTCAAATAACAATCAAACCATTTCTGGATGGGAGAATGGCGTGTGGAAAATGGAATCTA
  TATTCAGAAAAGTTGAAACAGACTGGCACATGGTATATTTGGCCCGAAAGGAAGGATCAT
  CTTTTGCTTATATTTCCTGGAAGTTTGAGTGTGGGTCAGTTGGCCTAAAAGTAGATAGCA
  TTTCTATTAGAACAAGTAGTCAAACTTTTCAGACTGGAACAGTAGAATGGAAATTGCGAT
  CTGATACAGCACAAGTAGAACTGACAGGCGATAACAGTCTTCACTCCTATGCTGATTTTT
  CTGGTGCCACTGAAGTTATTTTGGAAGCAGAATTAAGCAGAGGAGATGGTGATGTCGCTT
  GGCAACACACCCAGCTGTTTAGACAAAGCTTAAATGACCATGAAGAAAATTGTTTGGAGA
  TAATTATAAAATTCAGTGACCTTTGAGAACCTGAACATTATAGAAAAGCTGGCAATAATC
  AAGGACTTACTGAAGTAGTCTGTTGGTTCAGTGCATGCTTAGTTGGCAGTTACCACCCTG
  TGCTAGCATATTTCTTTTGCTAGCTATCCATCATGTAACCCTCATGAAAATTATCTTTAT
  ACGTGGACTATAATAAAATATTGAATTAAAACCTTAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018297.2 unedited
  GCGTTGTATACGACTCCTATAGGGCGGAGGGCAATCGGCAGAGGGGAGAGCGAGGGGCGG
  GGGAGGCGCCCGCTGGCGCTCAAGCATGGCGGCGGCGGCATTGGGCAGCTCCTCAGGCTC
  GGCGTCCCCGGCCGTGGCTGAGCTCTGCCAGAACACCCCGGAGACCTTTTTGGAGGCCTC
  CAAGCTGCTGCTCACCTATGCTGACAACATCCTCAGAAACCCTAATGATGAAAAATATAG
  ATCCATCCGGATTGGAAACACAGCCTTTTCTACTAGACTCTTGCCTGTCAGAGGAGCTGT
  TGAATGTTTATTTGAAATGGGCTTTGAAGAGGGAGAAACACATCTCATCTTTCCTAAAAA
  AGCTTCAGTGGAGCAGCTGCAAAAAATTCGTGACCTGATTGCCATAGAGAGAAGTAGCAG
  ACTGGATGGCTCAAATAAGAGCCACAAAGTAAAGTCATCTCAGCAACCTGCAGCCAGTAC
  CCAGCTTCCTACAACACCATCTTCAAATCCCAGTGGGTTAAACCAGCACACAAGGAACCG
  TCAAGGGCAGTCATCAGATCCACCATCTGCTTCAACGGTTGCTGCTGACTCAGCCATTCT
  AGAAGTTCTTCAGTCCAACATTCAGCATGTGCTGGTCTATGAAAATCCTGCTCTTCAGGA
  GAAAGCGTTGGCTTGTATTCCGGTCCAAGACTAAAAGGAAATCACAGAAAAGTTATCGAG
  AGCTAGAAATTGGATAAAGGTATCAATATAAGTGATGAGGATTTTCCTTTTGCTGGAGCT
  TTTGCACTGGTTTTAAGGAAGGATTTTTTCACTGGGGTGAATAACGTTTTGTGCAGCAAT
  GTGGTGGAAAGACTAGGTCTAGGAGATGATCCATTACTGCCATGATGATGACCTGAAGTG
  GGGTGCAAGGAAGTGGGAGAATCATAACTGAGATGACCTGGCCCGTTCACCATTCGCTTT
  CGA
• Restriction Sites: Please inquire
• ACCN: NM_018297
• ORF Size: 1965 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_018297.2, NP_060767.2
• RefSeq Size: 2182
• RefSeq ORF: 1965
• Locus ID: 55768
• Domains: TGc, PUG, PAW
• Gene Summary: This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.