MRPS18A (NM_018135) Human Untagged Clone

CAT#: SC113696

MRPS18A (untagged)-Human mitochondrial ribosomal protein S18A (MRPS18A), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPS18A
• Synonyms: HumanS18b; MRP-S18-3; MRP-S18-a; MRPS18-3; S18bmt; S18mt-a
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113696 sequence for NM_018135 edited (data generated by NextGen Sequencing)
  ATGGCGGCCCTCAAGGCTCTGGTGTCCGGCTGTGGGCGGCTTCTCCGTGGGCTACTAGCG
  GGCCCGGCAGCGACCAGCTGGTCTCGGCTTCCAGCTCGCGGGTTCAGGGAAGTGGTGGAG
  ACCCAAGAAGGGAAGACAACTATAATTGAAGGCCGTATCACAGCGACTCCCAAGGAGAGT
  CCAAATCCTCCTAACCCCTCTGGCCAGTGCCCCATCTGCCGTTGGAACCTGAAGCACAAG
  TATAACTATGACGATGTTCTGCTGCTTAGCCAGTTCATCCGGCCTCATGGAGGCATGCTG
  CCCCGAAAGATCACAGGCCTATGCCAGGAAGAACACCGCAAGATCGAGGAGTGTGTGAAG
  ATGGCCCACCGAGCAGGTCTATTACCAAATCACAGGCCTCGGCTTCCTGAAGGAGTTGTT
  CCGAAGAGCAAACCCCAACTCAACCGGTACCTGACGCGCTGGGCTCCTGGCTCCGTCAAG
  CCCATCTACAAAAAAGGCCCCCGCTGGAACAGGGTGCGCATGCCCGTGGGGTCACCCCTT
  CTGAGGGACAATGTCTGCTACTCAAGAACACCTTGGAAGCTGTATCACTGA
  
  Clone variation with respect to NM_018135.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018135 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGAAGAGGCGGCCCTCAAGGCTC
  TGGTGTCCGGCTGTGGGCGGCTTCTCCGTGGGCTACTAGCGGGCCCGGCAGCGACCAGCT
  GGTCTCGGCTTCCAGCTCGCGGGTTCAGGGAAGTGGTGGAGACCCAAGAAGGGAAGACAA
  CTATAATTGAAGGCCGTATCACAGCGACTCCCAAGGAGAGTCCAAATCCTCCTAACCCCT
  CTGGCCAGTGCCCCATCTGCCGTTGGAACCTGAAGCACAAGTATAACTATGACGATGTTC
  TGCTGCTTAGCCAGTTCATCCGGCCTCATGGAGGCATGCTGCCCCGAAAGATCACAGGCC
  TATGCCAGGAAGAACACCGCAAGATCGAGGAGTGTGTGAAGATGGCCCACCGAGCAGGTC
  TATTACCAAATCACAGGCCTCGGCTTCCTGAAGGAGTTGTTCCGAAGAGCAAACCCCAAC
  TCAACCGGTACCTGACGCGCTGGGCTCCTGGCTCCGTCAAGCCCATCTACAAAAAAGGCC
  CCCGCTGGAACAGGGTGCGCATGCCCGTGGGGTCACCCCTTCTGAGGGACANTGTCTGCT
  ACTCAAGAACACCTTGGAAGCTGTATCACTGACAGAGAGCAGTGCTTNCAGAGTTCCTTC
  TGCACCTGTGCTGGGGAGTANGAGGCCCACTCANCAGCCCTTTGGCCACACTATACTCCT
  GTCCCACCCCACACGATGGCCTGGTCCCTCCACATGCATGGACAGGNGACAGTGGGACTA
  ACTTCAGTACCCTTGGCCTGCACAGTANNCATGCTGGGAGCTANAGGCANGCAGGNCANN
  TGGNGTCCCTTNGCCAGCTGCTATGGGGCTTANGCCATGCTCAGTGCTGGGGACAAGGAN
  TTTTGCCCNACGCAGTGTCATAAACTGGGGTCATGGGNCTACCCATNGGNTGTGCGCTAC
  TGCTNGGAAGTGCGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018135 unedited
  GGTTGAAAGGCGCCGTANATGATTATCGAGTAGCCNNNNNNNNNNANANNAAATTTTTTT
  TTATATTTTTTTTTTTTTTATCTGTTTTTGTTTTTTTTTTCTTTTTTTGAAAAGATGGGA
  CCTGTTTGGCCCATGAATTCAATTGACTCATTGGCCCCATCAAAAAAAAACATGACTCAA
  TGGTTAACACCCAACTGGGAATTTTTCCAAAACCCCCTGGACTTTCCAAGGGGGGAGGGC
  CCCCCCAATGGGTAAACCCCTAAACCCCGTTTTTTACCCTGGGGTGGGGAAAAATCCTTT
  CCCCAGCACTGAAAATGGGCTAAACCCCAAAAAAGCTGGGAAGGGAACCAAATGGCCTGG
  CCGGCTTTAACTCCCAACATTGTTTCTTGGCCGGCCAAGGGTCCTGAAATTAATTCCACT
  GTCCCCTGTTCATGCCTGTTTGAGGGACCACACCCTTTTGGTGGGGGGGGACAGGAGTAT
  ATTTTTGACCCAAGGCTTTGTAGTGGGCCCCCCTCTCCCCACAAAGGTGCCGGAAAAACC
  CTTGAAACCCTGCTTTTTGTTCGAAATACAAATTCCAAGGGTTTTTTAGAAGAGAAATTT
  TTCCTTAAAAAGGGGGAACTCACGGGATTTCCCCCCCTTTTCTAAGGGGGGCCTTTTTTT
  TAAAGTGGTTTTATGGAACCCAAAACCCATAGCACAAGAAACTGGTGGGGTGTGGTTTAT
  TCTTACAACCACCCTTTCAAAAACCCCGGTCCTGATTTGTGAAAATAAAACTGGATGGGG
  GGCAATTTCAACACACCCGTGATATTTATGGGTCCTCTCGTGTAAGCCTGAGATCTTTTT
  GGAAGAAACTTCATGTAGCACGGTAAACGGTTTACACTCCAACACGCCTTATGTAAATTG
  CGCTCGGGCACCAGTGAGAAGGGCGCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018135
• ORF Size: 591 bp
• Insert Size: 1080
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_018135.2, NP_060605.1
• RefSeq Size: 1060
• RefSeq ORF: 591
• Locus ID: 55168
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. A pseudogene corresponding to this gene is found on chromosome 3p. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.