MRPS18A (NM_018135) Human Untagged Clone

CAT#: SC321075

MRPS18A (untagged)-Human mitochondrial ribosomal protein S18A (MRPS18A), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPS18A
• Synonyms: HumanS18b; MRP-S18-3; MRP-S18-a; MRPS18-3; S18bmt; S18mt-a
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_018135.2 GGGGGAAGATGGCGGCCCTCAAGGCTCTGGTGTCCGGCTGTGGGCGGCTTCTCCGTGGGC
  TACTAGCGGGCCCGGCAGCGACCAGCTGGTCTCGGCTTCCAGCTCGCGGGTTCAGGGAAG
  TGGTGGAGACCCAAGAAGGGAAGACAACTATAATTGAAGGCCGTATCACAGCGACTCCCA
  AGGAGAGTCCAAATCCTCCTAACCCCTCTGGCCAGTGCCCCATCTGCCGTTGGAACCTGA
  AGCACAAGTATAACTATGACGATGTTCTGCTGCTTAGCCAGTTCATCCGGCCTCATGGAG
  GCATGCTGCCCCGAAAGATCACAGGCCTATGCCAGGAAGAACACCGCAAGATCGAGGAGT
  GTGTGAAGATGGCCCACCGAGCAGGTCTATTACCAAATCACAGGCCTCGGCTTCCTGAAG
  GAGTTGTTCCGAAGAGCAAACCCCAACTCAACCGGTACCTGACGCGCTGGGCTCCTGGCT
  CCGTCAAGCCCATCTACAAAAAAGGCCCCCGCTGGAACAGGGTGCGCATGCCCGTGGGGT
  CACCCCTTCTGAGGGACAATGTCTGCTACTCAAGAACACCTTGGAAGCTGTATCACTGAC
  AGAGAGCAGTGCTTCCAGAGTTCCTCCTGCACCTGTGCTGGGGAGTAGGAGGCCCACTCA
  CAAGCCCTTGGCCACAACTATACTCCTGTCCCACCCCACCACGATGGCCTGGTCCCTCCA
  ACATGCATGGACAGGGGACAGTGGGACTAACTTCAGTACCCTTGGCCTGCACAGTAGCAA
  TGCTGGGAGCTAGAGGCAGGCAGGGCAGTTGGGTCCCTTGCCAGCTGCTATGGGGCTTAG
  GCCATGCTCAGTGCTGGGGACAGGAGTTTTGCCCAACGCAGTGTCATAAACTGGGTTCAT
  GGGCTTACCCATTGGGTGTGCGCTCACTGCTTGGGAAGTGCAGGGGGTCCTGGGCACATT
  GCCAGCTGGGTGCTGAGCATTGAGTCACTGATCTCTTGTGATGGGGCCAATGAGTCAATT
  GAATTCATGGGCCAAACAGGTCCCATCCTCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018135.2 unedited
  TGCCACTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGGCCATTACGGCCGGGGGAA
  GATGGCGGCCCTCAAGGCTCTGGTGTCCGGCTGTGGGCGGCTTCTCCGTGGGCTACTAGC
  GGGCCCGGCAGCGACCAGCTGGTCTCGGCTTCCAGCTCGCGGGTTCAGGGAAGTGGTGGA
  GACCCAAGAAGGGAAGACAACTATAATTGAAGGCCGTATCACAGCGACTCCCAAGGAGAG
  TCCAAATCCTCCTAACCCCTCTGGCCAGTGCCCCATCTGCCGTTGGAACCTGAAGCACAA
  GTATAACTATGACGATGTTCTGCTGCTTAGCCAGTTCATCCGGCCTCATGGAGGCATGCT
  GCCCCGAAAGATCACAGGCCTATGCCAGGAAGAACACCGCAAGATCGAGGAGTGTGTGAA
  GATGGCCCACCGAGCAGGTCTATTACCAAATCACAGGCCTCGGCTTCCTGAAGGAGTTGT
  TCCGAAGAGCAAACCCCAACTCAACCGGTACCTGACGCGCTGGGCTCCTGGCTCCGTCAA
  GCCCATCTACAAAAAAGGCCCCCGCTGGAACAGGGTGCGCATGCCCGTGGGGTCACCCCT
  TCTGAGGGACAATGTCTGCTACTCAAGAACACCTTGGAAGCTGTATCACTGACAGAGAGC
  AGTGCTTCCAGAGTTCCTCCTGCACCTGTGCTGGGGAGTAGGAGGCCCACTCACAAGCCC
  TTGGCCACAACTATACTCCTGTCCCACCCCCACCACGATGGCCTGGTCCCTCCAACATGC
  ATGGGACAGGGGACAGTGGACTAACTTCAGTACCCTTGCCCTGCACAGTAGCATGCTGGA
  GCTAGAGCAGCAGGCAGTTGGGTCCCTTGCCAGCTGCTATGGGCTAGCCATGCTCATGCT
  GGGACGGAGTTTGCCCACGCATGTCATAACTGGGTCATGGGCTACCAATTGGGTGGTGCG
  CTCACTGCTTGGGAGTGCCAGGT
• Restriction Sites: Please inquire
• ACCN: NM_018135
• ORF Size: 591 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_018135.2, NP_060605.1
• RefSeq Size: 1060
• RefSeq ORF: 591
• Locus ID: 55168
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. A pseudogene corresponding to this gene is found on chromosome 3p. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.